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The map label for this gene is cbiP [H]

Identifier: 29141348

GI number: 29141348

Start: 945995

End: 947515

Strand: Direct

Name: cbiP [H]

Synonym: t0855

Alternate gene names: 29141348

Gene position: 945995-947515 (Clockwise)

Preceding gene: 29141347

Following gene: 29141349

Centisome position: 19.74

GC content: 55.88

Gene sequence:

>1521_bases
ATGACGCAGGCAGTTATGTTGCAGGGGACGGCATCCGACGTCGGCAAAAGTGTGCTGGTGGCCGGTTTATGCCGCATTTT
TTATCAGGATGGTCTGCGTACCGCGCCGTTTAAATCGCAAAATATGGCGCTTAATTCTGGTATTACGCCGGATGGTAAAG
AGATGGGGCGGGCGCAGATTTTTCAGGCGGAAGCCGCGGGGATCACGCCAGATGTGCGTATGAATCCGGTGCTGCTCAAA
CCGACCAGCGATCGCCAGGCGCAGGTCGTGCTGATGGGGAAAGTGGCGACCAATATGGATGCGGTGAGTTACCATGACTA
TAAACCGCGTTTGCGTGAGCAAATCCTTGCGGTCTATAACAGCCTGGCGCAGGAATATGACGTTATTGTGCTGGAAGGCG
CCGGAAGCCCGGCGGAAATCAATCTGCGCGATCGCGATATCGTCAATATGGGAATGGCGGAAATGGCCCAGTGTCCGGTT
ATTCTGGTGGCGGATATCGACCGGGGCGGCGTATTCGCCGCTATTTATGGCACGCTGGCGCTACTGCACAAACAGGAGCG
TGACAGAGTAAAGGGCGTCATTATCAACAAATTCCGCGGCGACGTGGCGCTACTCTATTCCGGTATCGAACAAATCGAAT
CGCTTACCGGCGTCCCGGTTCTGGGAGTGATGCCATGGCTGGATGTGGATCTGGAAGATGAAGATGGCGTCGCGCTGCAA
AACGATAAATATCGCGGTAACGCCCCCCGTGATATCACTATTGCCATTGTGCAACTGCCACATATTTCTAACTTTACGGA
TTTTAACGCTCTGGCGGCGCAGCCGGATGTGCGCATACGCTATATTCGCCGCCCGGAAGCGTTGACTGACGCTGATCTGG
TGATCCTGCCGGGCAGTAAGAATACGCTGAGCGACCTCGCCTGGCTGCGCGAAAGCGGGATGGCGGATGCGGTATTGCAG
ACACATCGGCAGGGCGTGCCGGTGATGGGAATTTGCGGTGGCTACCAGATGCTGGGCGACACTATTGTGGATGAGGTGGA
GTCGGGGCTGGGTACGCAGCCGGGCCTGGGACTGCTTAATACGATCACTCGCTTTGCACAGGATAAAACCACGACGCAGG
TTAACGCGACAATGTCAGGCGAGCTGCCCGGCTGGCTGGCTGCCGCGGCGGGGCTACCGGTACGCGGTTATGAAATCCAC
ATGGGGGAAACGGTGCTGCAGGAAGGATGCTGTACGGCCATGACGTTACAGAAAAATGGATGCTCTGTGGCGGATGGCGC
GGTCACCGCCGACGGACTGGCGTTTGGCACTTACCTTCACGGTCTGTTTGACAGCGATGCCTTTACCCGTGCGGTGGTCA
ATGGGTTACGGGCGCGAAAAGGGCTTGCGCCGTGGGAAACGACTTTTTGTTATGCGGAGCATAAAGCGCGGCAATTTGAT
CTGCTGGCGGAAGCGATGCGGCAACATATTGATATTGATAAAATTTACACCATCATGCAACAGCATCAGGAGCCGGTATG
A

Upstream 100 bases:

>100_bases
CCTGGCTGGTCAAACTTCATACTCAACTGGGACTGCCGCTGTGTAAAACAGAAACGGAGTTTTTTCATCGAATGCAAAAG
TGCGCATTCAGGGAGGCGTC

Downstream 100 bases:

>100_bases
TGATTCTGGTGACGGGCGGGGCGCGTAGTGGTAAAAGCCGTCATGCTGAAGCCTTAATTAGCGATGCGCCGCAGGTACTG
TATATCGCCACCTCGCAGAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTQAVMLQGTASDVGKSVLVAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQIFQAEAAGITPDVRMNPVLLK
PTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYNSLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPV
ILVADIDRGGVFAAIYGTLALLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDADLVILPGSKNTLSDLAWLRESGMADAVLQ
THRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLNTITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIH
MGETVLQEGCCTAMTLQKNGCSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYAEHKARQFD
LLAEAMRQHIDIDKIYTIMQQHQEPV

Sequences:

>Translated_506_residues
MTQAVMLQGTASDVGKSVLVAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQIFQAEAAGITPDVRMNPVLLK
PTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYNSLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPV
ILVADIDRGGVFAAIYGTLALLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDADLVILPGSKNTLSDLAWLRESGMADAVLQ
THRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLNTITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIH
MGETVLQEGCCTAMTLQKNGCSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYAEHKARQFD
LLAEAMRQHIDIDKIYTIMQQHQEPV
>Mature_505_residues
TQAVMLQGTASDVGKSVLVAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQIFQAEAAGITPDVRMNPVLLKP
TSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYNSLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPVI
LVADIDRGGVFAAIYGTLALLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQN
DKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDADLVILPGSKNTLSDLAWLRESGMADAVLQT
HRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLNTITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIHM
GETVLQEGCCTAMTLQKNGCSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYAEHKARQFDL
LAEAMRQHIDIDKIYTIMQQHQEPV

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 54982; Mature: 54851

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAVMLQGTASDVGKSVLVAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQI
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHCHHHE
FQAEAAGITPDVRMNPVLLKPTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYN
EEHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEEHHCCCCCEEECCCCHHHHHHHHHHHH
SLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPVILVADIDRGGVFAAIYGTLA
HHHHHCCEEEEECCCCCCEEEECCCHHHHCCHHHHHCCCEEEEEECCCCCCHHHHHHHHH
LLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
HHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCEEE
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDADLVILPGSK
CCCCCCCCCCEEEEEEEEECCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCEEEECCCC
NTLSDLAWLRESGMADAVLQTHRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLN
CHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHH
TITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIHMGETVLQEGCCTAMTLQKNG
HHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEEECCC
CSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYAEHKARQFD
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHH
LLAEAMRQHIDIDKIYTIMQQHQEPV
HHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQAVMLQGTASDVGKSVLVAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQI
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHCHHHE
FQAEAAGITPDVRMNPVLLKPTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYN
EEHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEEHHCCCCCEEECCCCHHHHHHHHHHHH
SLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPVILVADIDRGGVFAAIYGTLA
HHHHHCCEEEEECCCCCCEEEECCCHHHHCCHHHHHCCCEEEEEECCCCCCHHHHHHHHH
LLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
HHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCEEE
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDADLVILPGSK
CCCCCCCCCCEEEEEEEEECCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCEEEECCCC
NTLSDLAWLRESGMADAVLQTHRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLN
CHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHH
TITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIHMGETVLQEGCCTAMTLQKNG
HHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEEECCC
CSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYAEHKARQFD
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHH
LLAEAMRQHIDIDKIYTIMQQHQEPV
HHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]