Click here to switch to the map view.

The map label for this gene is pduQ [H]

Identifier: 29141320

GI number: 29141320

Start: 918415

End: 919527

Strand: Reverse

Name: pduQ [H]

Synonym: t0822

Alternate gene names: 29141320

Gene position: 919527-918415 (Counterclockwise)

Preceding gene: 29141321

Following gene: 29141319

Centisome position: 19.19

GC content: 59.93

Gene sequence:

>1113_bases
ATGAACACCTTCTCACTACAAACGCGGTTGTACAGCGGTCAGGGCAGCCTGGCGGTGCTCAAGCGCTTTACCAATAAGCA
CATCTGGATAATCTGCGATGGCTTTCTGGCACGCTCGCCGCTGCTGGATACCCTGTGTAACGCGCTGCCCGCAGATAACC
GCATCAGCGTCTTTAGCGAGATAACGCCGGACCCCACCATCCATACCGTGGTGCAGGGCATTGCGCAAATGCAGGCCCTG
CAGCCGCAGGTGGTGATTGGTTTTGGCGGCGGCTCGGCAATGGACGCGGCGAAAGCGATTGTCTGGTTTAGCCAACAAAG
CGGCATCAATATCGAAACCTGCGTGGCGATCCCGACCACCAGCGGCACTGGTTCGGAAGTCACCAGCGCCTGCGTAATTA
GCGATCCGGATAAAGGCATCAAGTATCCGCTGTTCAACAATGCGCTGTATCCGGATATGGCGATCCTCGACCCGGAGCTG
GTGGTCAGCGTACCGCCGCAGATTACCGCCAACACCGGCATGGACGTGCTGACCCACGCCCTGGAGGCCTGGGTGTCACC
GCACGCCAGCGACTTTACCGACGCGCTGGCGGAAAAAGCCGCCAAACTGGTGTTCCAGTATCTGCCGACGGCGGTGGAAA
AAGGCGACTGCGTGGCGACGCGAGGAAAAATGCATAACGCCTCAACGCTCGCCGGGATGGCCTTCAGCCAGGCGGGGCTG
GGGCTTAACCACGCGATAGCCCACCAGCTTGGCGGACAGTTTCATCTTCCGCATGGCCTGGCCAATGCGCTGCTGCTCAC
GACGGTGATCCGTTTTAACGCCGGAGTTCCGCGCGCAGCCAAACGCTACGCGCGGCTGGCCAAAGCCTGCGGTTTTTGCC
CGGCAGAAGCCAACGATGTCACGGCGATCAATGCGCTGATTCAGCAAATCGAACTGCTTAAGCAACGCTGTGCCCTTCCC
TCACTGGCCGTTGCGCTAAAAGAAGGAAGATCCGACTTTTCCGCACGTATTCCAGCGATGGTGCAGGCCGCGCTGGCGGA
TGTCACGTTGCGCACCAACCCGCGCCCGGCCAGCGCCGAGGAAATTCGTGAACTGCTGGAGGAACTGCTATGA

Upstream 100 bases:

>100_bases
CCACGCCAACCGGTGAAGGAACAACGTCAGCGCGTACTTTTGCCCGCTCCCGGCGCTGCGTGCTGACCAACGGCTTTTCT
ATTCGCTAACCGAGGTCGCG

Downstream 100 bases:

>100_bases
GCGCCGCCATCAACAGCGTTTAAATGTCCCTTAGCGCCGATGAAATTCGTGAGCGCGTTCGCGCAGCGGGCGTTGTCGGT
GCGGGCGGCGCAGGTTTTCC

Product: propanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLCNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDVTAINALIQQIELLKQRCALP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPASAEEIRELLEELL

Sequences:

>Translated_370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLCNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDVTAINALIQQIELLKQRCALP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPASAEEIRELLEELL
>Mature_370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLCNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDVTAINALIQQIELLKQRCALP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPASAEEIRELLEELL

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=405, Percent_Identity=25.9259259259259, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787493, Length=406, Percent_Identity=35.9605911330049, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082107, Length=382, Percent_Identity=39.0052356020942, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI48994951, Length=348, Percent_Identity=37.0689655172414, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789163, Length=383, Percent_Identity=32.3759791122715, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789386, Length=344, Percent_Identity=26.1627906976744, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=366, Percent_Identity=24.8633879781421, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=329, Percent_Identity=33.434650455927, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24657991, Length=407, Percent_Identity=24.8157248157248, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 39436; Mature: 39436

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLCNALPADNRISVFSE
CCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHC
ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEEEEECC
SGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELVVSVPPQITANTGMDVLTHA
CCCCHHHHCEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHHHHH
LEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDV
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCH
TAINALIQQIELLKQRCALPSLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPASAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHH
EIRELLEELL
HHHHHHHHHC
>Mature Secondary Structure
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLCNALPADNRISVFSE
CCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHC
ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEEEEECC
SGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELVVSVPPQITANTGMDVLTHA
CCCCHHHHCEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHHHHH
LEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDV
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCH
TAINALIQQIELLKQRCALPSLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPASAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHH
EIRELLEELL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]