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The map label for this gene is pduW [H]

Identifier: 29141315

GI number: 29141315

Start: 914500

End: 915714

Strand: Reverse

Name: pduW [H]

Synonym: t0817

Alternate gene names: 29141315

Gene position: 915714-914500 (Counterclockwise)

Preceding gene: 29141316

Following gene: 29141314

Centisome position: 19.11

GC content: 52.67

Gene sequence:

>1215_bases
ATGTCTTACAAAATAATGGCCATTAACGCCGGCAGCTCATCGTTAAAGTTCCAGCTGCTGGAGATGCCGCAGGGCGACAT
GCTCTGCCAGGGGCTGATTGAGCGTATCGGTATGGCGGATGCGCAGGTGACAATTAAGACGCACAGCCAAAAATGGCAGG
AAACCGTCCCCGTTGCCGATCATCGCGATGCGGTAACGCTGCTTCTGGAAAAGCTGCTGGGTTATCAAATCATTAACAGT
CTGCGGGACATTGACGGGGTGGGCCATCGTGTGGCGCACGGCGGTGAGTTTTTTAAAGACTCGACGCTGGTCACGGATGA
AACGTTAGCGCAAATCGAACGTCTTGCTGAACTGGCCCCGCTTCACAACCCGGTAAACGCGTTGGGTATTCACGTTTTTC
GTCAACTGTTGCCTGATGCCCCTTCTGTAGCCGTGTTTGATACGGCATTTCACCAGACGCTGGATGAACCCGCCTATATT
TACCCGCTTCCCTGGCATTATTACGCTGAACTGGGGATTCGCCGTTATGGCTTTCATGGCACCAGCCATAAGTATGTCAG
CGGCGTTCTGGCTGAAAAGCTGGGCGTACCACTCAGCGCATTACGGGTGATTTGTTGTCATCTTGGAAACGGTAGCAGCG
TCTGCGCCATCAAGAACGGACGTTCGGTCAATACGTCGATGGGCTTTACGCCGCAGTCAGGCGTGATGATGGGCACCCGT
AGCGGAGATATTGATCCCTCTATTCTGCCGTGGATAGCCCAGCGCGAATGCAAAACGCCGCAACAGTTGAATCAGTTATT
AAATAATGAGTCAGGCTTGCTGGGCGTTTCTGGCGTGTCGTCTGATTACCGCGATGTCGAACAGGCAGCCAATACCGGTA
ACCGGCAGGCTAAACTCGCTCTCACGCTGTTTGCCGAACGCATTCGCGCCACCATTGGTGGCTATATTATGCAAATGGGA
GGACTGGATGCTCTGGTATTCACTGGGGGAATTGGCGAGAACTCAGCGCGCGCCAGATCGGCGGTTTGCCATAATTTGCA
ATTTTTAGGCTTAGCCGTTGATGAAGAGAAAAATCAACGTAATGCAACATTTATCCAGACGGAGAACGCCCTGGTGAAAG
TCGCCGTCATCAACACCAACGAAGAGTTAATGATCGCACAAGACGTTATGCGCATTGCGCTACCGGCCACGGAAGGGCTT
TGTGTACCAGCCTGA

Upstream 100 bases:

>100_bases
GGCTTACGCAGGCTGGCGCCCGGCAGATTTTTATTACCAGCGCGTTGAATAATTCGGGTCTCGACGCCGTGCTGGATTTT
CTGAATTCGAAGGAACCTTT

Downstream 100 bases:

>100_bases
GAGGGGTATACGACGCATTGCGGCAAGTCATGGTAATAACGCTTTTACCATGACACTATGCGCGCACACTATTCGTACCT
GAAAGGTGATAACGTGGCTG

Product: propionate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSYKIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHSQKWQETVPVADHRDAVTLLLEKLLGYQIINS
LRDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAPLHNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYI
YPLPWHYYAELGIRRYGFHGTSHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTR
SGDIDPSILPWIAQRECKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAANTGNRQAKLALTLFAERIRATIGGYIMQMG
GLDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQRNATFIQTENALVKVAVINTNEELMIAQDVMRIALPATEGL
CVPA

Sequences:

>Translated_404_residues
MSYKIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHSQKWQETVPVADHRDAVTLLLEKLLGYQIINS
LRDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAPLHNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYI
YPLPWHYYAELGIRRYGFHGTSHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTR
SGDIDPSILPWIAQRECKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAANTGNRQAKLALTLFAERIRATIGGYIMQMG
GLDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQRNATFIQTENALVKVAVINTNEELMIAQDVMRIALPATEGL
CVPA
>Mature_403_residues
SYKIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHSQKWQETVPVADHRDAVTLLLEKLLGYQIINSL
RDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAPLHNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYIY
PLPWHYYAELGIRRYGFHGTSHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTRS
GDIDPSILPWIAQRECKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAANTGNRQAKLALTLFAERIRATIGGYIMQMGG
LDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQRNATFIQTENALVKVAVINTNEELMIAQDVMRIALPATEGLC
VPA

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family. PduW subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=43.9698492462312, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788633, Length=401, Percent_Identity=42.643391521197, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.15 [H]

Molecular weight: Translated: 43968; Mature: 43837

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHSQKWQETVPVAD
CCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCC
HRDAVTLLLEKLLGYQIINSLRDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHCCCCEECHHHHHHHHHHHHHHC
LHNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYIYPLPWHYYAELGIRRYGFHG
CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEEEECCHHHHHHHCHHHCCCCC
TSHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTR
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCCCCCCEEEECC
SGDIDPSILPWIAQRECKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAANTGNRQAKLA
CCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHH
LTLFAERIRATIGGYIMQMGGLDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQR
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCC
NATFIQTENALVKVAVINTNEELMIAQDVMRIALPATEGLCVPA
CCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SYKIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHSQKWQETVPVAD
CEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCC
HRDAVTLLLEKLLGYQIINSLRDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHCCCCEECHHHHHHHHHHHHHHC
LHNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYIYPLPWHYYAELGIRRYGFHG
CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEEEECCHHHHHHHCHHHCCCCC
TSHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTR
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCCCCCCEEEECC
SGDIDPSILPWIAQRECKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAANTGNRQAKLA
CCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHH
LTLFAERIRATIGGYIMQMGGLDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQR
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCC
NATFIQTENALVKVAVINTNEELMIAQDVMRIALPATEGLCVPA
CCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA