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The map label for this gene is maiA [H]

Identifier: 29141186

GI number: 29141186

Start: 764731

End: 765375

Strand: Direct

Name: maiA [H]

Synonym: t0679

Alternate gene names: 29141186

Gene position: 764731-765375 (Clockwise)

Preceding gene: 29141185

Following gene: 29141187

Centisome position: 15.96

GC content: 56.12

Gene sequence:

>645_bases
ATGAAGCTGTACAGTTTCTTTAATAGTTCGGCGTCTTATCGTGTACGTATTGCGCTGGCGTTAAAGGGGATTGATTACCA
GACAGTGGGCGTCAATATTCGTATCGGCCAGCAGAATGCGCTGGCCTACCGACGGATGAATCCGGTAGGCCTGGTGCCGA
CGCTAGTCACTGATGACGGCGAATCGTTGGGGCAATCGCTGGCCATCGTCGACTGGCTGGACCGACATTTTCCGCAAACG
CCGCTGCTGCCGACGAGCGACCCGGCGCGCAGCCAGGCGCTGGAAATTGTCTACGCTATCGCCTGCGATATACACCCGAT
CAATAACATGCGCGTGTTGCGCTACCTGACCGATGAGCTGAAGGTTAGCGAAGAGGATAAAAAACGCTGGTACGCCCACT
GGATACAGCAAGGGTTAAGCGCGGTAGAGCAGCTATTGCGTAAAAGTCAGTCCGGACGATTCTGCGTGGGCGACGCGCCA
GGCCTTGCGGACTGCTGCCTGATTCCTCAGTGGGCGAATGCGCTCAGGATGGGCTGCGATTTATCAGGCTATCCGCGCTG
TAAAGCCGTTTATGACGCCTGCGTACAGCTACCGGCGTTTATTGCCGCCGCGCCGGAAAATCAACAAGATAAAATCCCGG
CCTGA

Upstream 100 bases:

>100_bases
GTACGCCAGAGGGGGTGGGCGCAGTGGTGAAAGGGGATGTCATCACCGGGAACGTAGAAGGTCTGACGCCGATTGCTGTG
AAAATTGTCTGAGGTGAACG

Downstream 100 bases:

>100_bases
AAAGGAGAATGACCATGACTCACGTGACAAGCGCAATTATTGTAGGTGGCGGAATCGGCGGCGCGGCGACCGCGCTGTCG
CTGGCGCGCCAGGGAATAAA

Product: glutathione-S-transferase-family protein

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSFFNSSASYRVRIALALKGIDYQTVGVNIRIGQQNALAYRRMNPVGLVPTLVTDDGESLGQSLAIVDWLDRHFPQT
PLLPTSDPARSQALEIVYAIACDIHPINNMRVLRYLTDELKVSEEDKKRWYAHWIQQGLSAVEQLLRKSQSGRFCVGDAP
GLADCCLIPQWANALRMGCDLSGYPRCKAVYDACVQLPAFIAAAPENQQDKIPA

Sequences:

>Translated_214_residues
MKLYSFFNSSASYRVRIALALKGIDYQTVGVNIRIGQQNALAYRRMNPVGLVPTLVTDDGESLGQSLAIVDWLDRHFPQT
PLLPTSDPARSQALEIVYAIACDIHPINNMRVLRYLTDELKVSEEDKKRWYAHWIQQGLSAVEQLLRKSQSGRFCVGDAP
GLADCCLIPQWANALRMGCDLSGYPRCKAVYDACVQLPAFIAAAPENQQDKIPA
>Mature_214_residues
MKLYSFFNSSASYRVRIALALKGIDYQTVGVNIRIGQQNALAYRRMNPVGLVPTLVTDDGESLGQSLAIVDWLDRHFPQT
PLLPTSDPARSQALEIVYAIACDIHPINNMRVLRYLTDELKVSEEDKKRWYAHWIQQGLSAVEQLLRKSQSGRFCVGDAP
GLADCCLIPQWANALRMGCDLSGYPRCKAVYDACVQLPAFIAAAPENQQDKIPA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=41.4285714285714, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=34.7619047619048, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=37.1069182389937, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=37.9807692307692, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=35.4066985645933, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17556142, Length=209, Percent_Identity=36.3636363636364, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645375, Length=213, Percent_Identity=42.7230046948357, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553325, Length=213, Percent_Identity=42.7230046948357, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21355859, Length=213, Percent_Identity=42.7230046948357, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355857, Length=214, Percent_Identity=37.8504672897196, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23842; Mature: 23842

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSFFNSSASYRVRIALALKGIDYQTVGVNIRIGQQNALAYRRMNPVGLVPTLVTDDG
CCCEEECCCCCCEEEEEEEEEECCCEEEEEEEEEECCCCHHHHHCCCCCCCEEEEEECCC
ESLGQSLAIVDWLDRHFPQTPLLPTSDPARSQALEIVYAIACDIHPINNMRVLRYLTDEL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHH
KVSEEDKKRWYAHWIQQGLSAVEQLLRKSQSGRFCVGDAPGLADCCLIPQWANALRMGCD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCHHHHHHHHHCCC
LSGYPRCKAVYDACVQLPAFIAAAPENQQDKIPA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLYSFFNSSASYRVRIALALKGIDYQTVGVNIRIGQQNALAYRRMNPVGLVPTLVTDDG
CCCEEECCCCCCEEEEEEEEEECCCEEEEEEEEEECCCCHHHHHCCCCCCCEEEEEECCC
ESLGQSLAIVDWLDRHFPQTPLLPTSDPARSQALEIVYAIACDIHPINNMRVLRYLTDEL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHH
KVSEEDKKRWYAHWIQQGLSAVEQLLRKSQSGRFCVGDAPGLADCCLIPQWANALRMGCD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCHHHHHHHHHCCC
LSGYPRCKAVYDACVQLPAFIAAAPENQQDKIPA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]