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The map label for this gene is yeiT

Identifier: 29141176

GI number: 29141176

Start: 755720

End: 756961

Strand: Reverse

Name: yeiT

Synonym: t0669

Alternate gene names: 29141176

Gene position: 756961-755720 (Counterclockwise)

Preceding gene: 29141177

Following gene: 29141170

Centisome position: 15.8

GC content: 50.72

Gene sequence:

>1242_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACGCCTGGTTTTACGCCATTACTGGCAATAAAAGAGGCTTCCCGTTGTTT
ATTATGTCACGATGCGCCCTGTAGTCAGGCTTGCCCGGCGCAAACCGATCCGGGGAAATTTATTCGTTCTATTTACTTTC
GCAATTTTAAAGGCGCAGCGGAAACTATCCGGGAAAATAATGCGCTTGGCGCCGTCTGCGCCAGAGTCTGTCCGACGGAA
AAATTATGCCAGCGCGGATGTACACGTTCCGGAATAGATAAACCTATTGATATTGCACGCCTGCAACGTTTTATTACCGA
TTTTGAACAGCAGACTGCCATGCAGATTTATCAGCCCGGGAGTAAAACACGCGGGAAAGTCGCGATTATTGGCGCGGGTC
CGGCAGGTTTGCAGGCAAGCGTGACGCTCACACATTTAGGCTATGACGTCACCATTTACGAAAAACAGCCGCAGCCCGGC
GGATGGCTGCGTCACGGCATCCCGGCATTTCGTCTGCCGCAAAGCGTACTCGATCAGGAAATTGCCCGCATTGTAGAAAT
GGGCGTCAATATTAAATGTAATTGTGACGTGGGCGGGTCGCTATCGCTTGCGCAACTCAAAGCCGAATATCGCGCCGTGC
TGATGACCGTCGGGATGTCCTGTGGCTCTGATTTACCGTTATTTGAGCAAGCCAGTCACGTAGAGATTGCCGTTGATTTT
TTACAGCGCGCCCGTCAGGCCGACGGCGATATTAGCGTTCCGCGTAGCGCATTAATTATTGGCGGCGGCGACGTAGCGAT
GGATGTCGCCAGTACGTTAAAAATTCTCGGCTGTCCTTCCGTCACCTGCGTGGCGCGGGAAGAATTAGCCGAATTCCCCG
CCAGCGAAAAAGAATTTACCAGCACGCAGGCATTAGGCGTATCGATTATTGATGGTTTTACGCCTGTCGCCGTCAGCGGA
AATAAAGTGACCTTCCACCATGTACGCCACTCAGGAGAACTGACGCTGGAAGCGGAAAATATTATTTTGGCCGTGGGGCA
GCACGCGCGACTGGATAACTTTGCGGAGATAAAAGCGCAGCATAATATTATCGACACGCACAATTATCAAACCGACGACC
CGGCGATCTTTGCCGCTGGCGATATTGTTAAAGGTGATAAGACCGTCGTTTATGCGGTAAAAACCGGAAAAGAAGCCGCT
CAGGCTATTCATCATTATTTAGAGGAGGCCTGCTCATGTTAA

Upstream 100 bases:

>100_bases
ATGCCCTTCTTTCAATGCTTAGGCTGGCGGTAGATTTTAGATCATTAAAACGCGTTATTTGCGTTTCGCCTTAATTTATT
CAAGCATTAAGGAAGATTAT

Downstream 100 bases:

>100_bases
CAAAAGATTTGTCTGTTACCTTTTGCGGCGTTAAGTTTCCCAATCCGTTTTGCCTTTCTTCTTCTCCGGTAGGCAATTGT
TATGAGATGTGCGCCAAAGC

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPGSKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPG
GWLRHGIPAFRLPQSVLDQEIARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTVGMSCGSDLPLFEQASHVEIAVDF
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFTSTQALGVSIIDGFTPVAVSG
NKVTFHHVRHSGELTLEAENIILAVGQHARLDNFAEIKAQHNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAA
QAIHHYLEEACSC

Sequences:

>Translated_413_residues
MPQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPGSKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPG
GWLRHGIPAFRLPQSVLDQEIARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTVGMSCGSDLPLFEQASHVEIAVDF
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFTSTQALGVSIIDGFTPVAVSG
NKVTFHHVRHSGELTLEAENIILAVGQHARLDNFAEIKAQHNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAA
QAIHHYLEEACSC
>Mature_412_residues
PQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPGSKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPGG
WLRHGIPAFRLPQSVLDQEIARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTVGMSCGSDLPLFEQASHVEIAVDFL
QRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFTSTQALGVSIIDGFTPVAVSGN
KVTFHHVRHSGELTLEAENIILAVGQHARLDNFAEIKAQHNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAAQ
AIHHYLEEACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=30.6636155606407, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=46.6666666666667, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1788468, Length=413, Percent_Identity=83.5351089588378, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=450, Percent_Identity=31.5555555555556, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789606, Length=445, Percent_Identity=31.0112359550562, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=30.4444444444444, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789243, Length=399, Percent_Identity=27.8195488721804, Blast_Score=128, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71984108, Length=443, Percent_Identity=31.3769751693002, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17570289, Length=446, Percent_Identity=27.1300448430493, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320030, Length=463, Percent_Identity=23.7580993520518, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24640763, Length=433, Percent_Identity=28.8683602771363, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI18858217, Length=433, Percent_Identity=28.8683602771363, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=28.1531531531532, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=28.1531531531532, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI28574881, Length=444, Percent_Identity=28.1531531531532, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24665539, Length=444, Percent_Identity=28.1531531531532, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEIT_SALTI (Q8Z5A6)

Other databases:

- EMBL:   AL627273
- EMBL:   AE014613
- RefSeq:   NP_456745.1
- RefSeq:   NP_804518.1
- ProteinModelPortal:   Q8Z5A6
- SMR:   Q8Z5A6
- GeneID:   1069835
- GeneID:   1248748
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0669
- KEGG:   sty:STY2416
- HOGENOM:   HBG715033
- OMA:   VIDYGRC
- ProtClustDB:   PRK11749
- BioCyc:   SENT209261:T0669-MONOMER
- BioCyc:   SENT220341:STY2416-MONOMER
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103
- Gene3D:   G3DSA:1.10.1060.10
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00368
- PRINTS:   PR00469

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; SSF46548 Helical_ferredxn

EC number: NA

Molecular weight: Translated: 44594; Mature: 44463

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPGGWLRHGIPAFRLPQSVLDQE
CCCCCEEEEEECCCCCCEEEEEEEEECEEEEEEECCCCCCCHHHHCCCHHCCCHHHHHHH
IARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTVGMSCGSDLPLFEQASHVEIAVDF
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHH
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFT
HHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHH
STQALGVSIIDGFTPVAVSGNKVTFHHVRHSGELTLEAENIILAVGQHARLDNFAEIKAQ
HHHHCCEEEECCCCCEEECCCEEEEEEEECCCCEEEEECCEEEEECCCCCCCCHHHHHHH
HNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAAQAIHHYLEEACSC
CCEEECCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPGGWLRHGIPAFRLPQSVLDQE
CCCCCEEEEEECCCCCCEEEEEEEEECEEEEEEECCCCCCCHHHHCCCHHCCCHHHHHHH
IARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTVGMSCGSDLPLFEQASHVEIAVDF
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHH
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFT
HHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHH
STQALGVSIIDGFTPVAVSGNKVTFHHVRHSGELTLEAENIILAVGQHARLDNFAEIKAQ
HHHHCCEEEECCCCCEEECCCEEEEEEEECCCCEEEEECCEEEEECCCCCCCCHHHHHHH
HNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAAQAIHHYLEEACSC
CCEEECCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504