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The map label for this gene is nfo [H]

Identifier: 29141162

GI number: 29141162

Start: 734840

End: 735697

Strand: Reverse

Name: nfo [H]

Synonym: t0652

Alternate gene names: 29141162

Gene position: 735697-734840 (Counterclockwise)

Preceding gene: 29141163

Following gene: 29141158

Centisome position: 15.35

GC content: 53.73

Gene sequence:

>858_bases
ATGAAATACATCGGAGCGCACGTCAGCGCTGCTGGCGGTCTGGCTAACGCCCCTGCCCGCGCGGCTGAAATTGGCGCAAC
GGCCTTTGCGCTTTTCACAAAAAACCAGCGTCAGTGGCGTGCCGCCCCCCTTACTCCCCAGGTCATTGATGACTTTAAAA
TCGCCTGTGAAAAGTATCATTTCTCGGCGGCGCAAATCCTTCCCCACGATAGTTACCTGATTAATCTGGGCCATCCGGTC
AGTGAAGCGCTGGAAAAATCACGCGATGCCTTTCTCGATGAAATGCAGCGCTGTGAACAACTCGGCTTAACCTTGCTTAA
TTTTCATCCCGGTAGCCATCTGATGCAGATTGCACAGGAGGATTGCCTGGCGCGGATCGCGGAATCTATCAATATTGCCC
TCGCGCAGACCGAGGGCGTTACGGCGGTTATCGAAAATACAGCCGGTCAGGGCAGTAATCTGGGGTTTGAGTTTGAACAG
TTAGCCGCCATCATCGACGGCGTGGAAGATAAGTCGCGCGTTGGCGTCTGTATCGATACCTGCCATGCCTTTGCCGCTGG
ATACGATCTGCGTACGCCAGAGGCGTGCGAAAAAACGTTCGCCGAATTCGGGAAAATTGTCGGATTTCAGTATTTGCGCG
GAATGCACCTTAACGACGCCAAAAGCGCCTTCGGTAGCCGCGTTGACCGCCATCACAGTCTGGGTGAAGGCAATATCGGC
CACGATGCGTTTCGTTGGATTATGCAGGATGCGCGTTTTGACGGTATTCCGCTGATACTGGAGACCATCAATCCTGATAT
CTGGGCGGAAGAGATTGCGTGGTTAAAAGCCCAGCAAATTGCCGAAGCGATGGCCTGA

Upstream 100 bases:

>100_bases
AATCCATAGCCTTATCGCATAAATTATTACGTTTCACGCCTGTTAACCCGTTATCATAGCGTTCGTAATGAACCGTTAGC
GGGTTTAACAGGAGTCTTTT

Downstream 100 bases:

>100_bases
CGCTCAATGTTCTTCAGGCAATAAAAAAGGCAGAGCGCGCTCTGCCTTTTTTAGCGTGTTTCTCTTATGCCGCTTTTGCC
GCGACTTCCGTCTCCGGACG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYHFSAAQILPHDSYLINLGHPV
SEALEKSRDAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQEDCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQ
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPEACEKTFAEFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAMA

Sequences:

>Translated_285_residues
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYHFSAAQILPHDSYLINLGHPV
SEALEKSRDAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQEDCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQ
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPEACEKTFAEFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAMA
>Mature_285_residues
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYHFSAAQILPHDSYLINLGHPV
SEALEKSRDAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQEDCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQ
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPEACEKTFAEFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAMA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=285, Percent_Identity=88.4210526315789, Blast_Score=534, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=50.1915708812261, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6322735, Length=287, Percent_Identity=42.1602787456446, Blast_Score=242, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31211; Mature: 31211

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYH
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
FSAAQILPHDSYLINLGHPVSEALEKSRDAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQE
CHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHH
DCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRTPEACEKTFAEFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAMA
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYH
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
FSAAQILPHDSYLINLGHPVSEALEKSRDAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQE
CHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHH
DCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRTPEACEKTFAEFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAMA
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA