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The map label for this gene is yeiO [H]

Identifier: 29141158

GI number: 29141158

Start: 729451

End: 730632

Strand: Reverse

Name: yeiO [H]

Synonym: t0648

Alternate gene names: 29141158

Gene position: 730632-729451 (Counterclockwise)

Preceding gene: 29141162

Following gene: 29141155

Centisome position: 15.25

GC content: 54.15

Gene sequence:

>1182_bases
ATGCACAACAGTCCCGCCGCCGCTGCGCCCAAATCGTTTGACCTGACGTCTACCGCCTTTTTGATCGTCGCTTTTCTCAC
CGGTATTGCTGGTGCGTTACAGACGCCGACACTCAGTATTTTTCTGACGGATGAGGTTCACGCCCGTCCCGGCATGGTGG
GTTTCTTTTTTACCGGCAGCGCGGTTATCGGCATTATCGTGAGCCAGTTTCTGGCGGGTCGCTCAGATAAAAAAGGCGAT
CGCAAAAAACTGATCGTTTTCTGTTGCGTGCTGGGCATGTTGGCCTGCGTGTTGTTTGCCTGGAACCGCAACTACTTTAT
TTTGCTGTTCATCGGCGTCTTTCTCAGTAGCTTCGGCTCTACCGCCAACCCGCAGATGTTTGCGCTGGCCCGAGAACATG
CCGATCGTACAGGTCGTGAAGCGGTGATGTTCAGCTCAATCCTCCGCGCCCAGGTCTCGTTGGCCTGGGTCATTGGGCCG
CCGCTGGCCTATGCGCTGGCGATGGGTTTTAGCTTTACGGTGATGTATTTGAGCGCGGCGGTAGCTTTTATCGTCTGCGG
CGTCATGGTGTGGTTCTTTTTACCGTCAATGCGCAAAGATGCGCCGCTGGCAACCGGCACGCTGGAAGCACCGCGTCGTA
ATCGCCGCGATACGCTGTTGCTGTTTGTCATCTGTACGTTAATGTGGGGGACTAACAGCCTGTATATCATTAATATGCCG
CTGTTTATCATCAATGAGCTGCATCTTCCGGAAAAACTTGCAGGCGTGATGATGGGCACCGCCGCCGGGCTGGAGATTCC
CACAATGCTGATTGCAGGTTACTTCGCCAAACGGTTGGGAAAACGCCTGTTAATGTGCATCGCCGTTGTCGCCGGACTGT
GCTTTTATGCGGGGATGTTGCTGGCGCACGCACCGGCAACGTTGCTCGGCTTGCAGTTGCTGAACGCGATTTACATCGGC
ATTCTTGGCGGGATTGGCATGCTCTATTTTCAGGATCTCATGCCAGGCCAGGCGGGTTCAGCCACCACGCTGTATACCAA
TACCATTCGCGTCGGCTGGATCATTGCCGGTTCGTTGGCGGGAATCGCCGCAGAAATCTGGAATTATCATGCGGTCTTCT
GGTTTGCGTTAGTTATGATTGTCGCCACCATGTTCTGCCTGGCGCGTATTAAAGATGTTTAA

Upstream 100 bases:

>100_bases
TGCTATTTTGATTTTTCGTGTCGATTTTGATTCAGACGCCTGTTTATTTTCTGACGGGCAGCGTAAACTCCGCCTCTCTT
CACACCACTGATATAGAAAC

Downstream 100 bases:

>100_bases
CTTGCAAGCCTGATACTTTTACTCTTCGACACATTTCAGCGCAGAATTTATTTCTGCGCGATGTTCATGCGCGGTCATTC
ATTGTCTTTTTCTCAATACG

Product: sugar efflux transporter

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MHNSPAAAAPKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTDEVHARPGMVGFFFTGSAVIGIIVSQFLAGRSDKKGD
RKKLIVFCCVLGMLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWFFLPSMRKDAPLATGTLEAPRRNRRDTLLLFVICTLMWGTNSLYIINMP
LFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMCIAVVAGLCFYAGMLLAHAPATLLGLQLLNAIYIG
ILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLAGIAAEIWNYHAVFWFALVMIVATMFCLARIKDV

Sequences:

>Translated_393_residues
MHNSPAAAAPKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTDEVHARPGMVGFFFTGSAVIGIIVSQFLAGRSDKKGD
RKKLIVFCCVLGMLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWFFLPSMRKDAPLATGTLEAPRRNRRDTLLLFVICTLMWGTNSLYIINMP
LFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMCIAVVAGLCFYAGMLLAHAPATLLGLQLLNAIYIG
ILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLAGIAAEIWNYHAVFWFALVMIVATMFCLARIKDV
>Mature_393_residues
MHNSPAAAAPKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTDEVHARPGMVGFFFTGSAVIGIIVSQFLAGRSDKKGD
RKKLIVFCCVLGMLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWFFLPSMRKDAPLATGTLEAPRRNRRDTLLLFVICTLMWGTNSLYIINMP
LFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMCIAVVAGLCFYAGMLLAHAPATLLGLQLLNAIYIG
ILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLAGIAAEIWNYHAVFWFALVMIVATMFCLARIKDV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport lactose and glucose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI1788495, Length=393, Percent_Identity=90.3307888040712, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1790091, Length=393, Percent_Identity=69.9745547073791, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI48994875, Length=376, Percent_Identity=53.4574468085106, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42623; Mature: 42623

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNSPAAAAPKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTDEVHARPGMVGFFFTGS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEHHH
AVIGIIVSQFLAGRSDKKGDRKKLIVFCCVLGMLACVLFAWNRNYFILLFIGVFLSSFGS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCC
TANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPLAYALAMGFSFTVMYLSAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHH
VAFIVCGVMVWFFLPSMRKDAPLATGTLEAPRRNRRDTLLLFVICTLMWGTNSLYIINMP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECH
LFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMCIAVVAGLCFYAGML
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHAPATLLGLQLLNAIYIGILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEHEEHHHHH
GIAAEIWNYHAVFWFALVMIVATMFCLARIKDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHNSPAAAAPKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTDEVHARPGMVGFFFTGS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEHHH
AVIGIIVSQFLAGRSDKKGDRKKLIVFCCVLGMLACVLFAWNRNYFILLFIGVFLSSFGS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCC
TANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPLAYALAMGFSFTVMYLSAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHH
VAFIVCGVMVWFFLPSMRKDAPLATGTLEAPRRNRRDTLLLFVICTLMWGTNSLYIINMP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECH
LFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMCIAVVAGLCFYAGML
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHAPATLLGLQLLNAIYIGILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEHEEHHHHH
GIAAEIWNYHAVFWFALVMIVATMFCLARIKDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10209755; 10438463 [H]