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The map label for this gene is ipdC [H]

Identifier: 29140973

GI number: 29140973

Start: 522062

End: 523714

Strand: Direct

Name: ipdC [H]

Synonym: t0452

Alternate gene names: 29140973

Gene position: 522062-523714 (Clockwise)

Preceding gene: 29140970

Following gene: 29140975

Centisome position: 10.89

GC content: 59.23

Gene sequence:

>1653_bases
ATGCAAAACCCCTATACCGTGGCCGACTATTTGCTGGACAGACTGGCAGGATGCGGCATTGGCCATCTTTTTGGCGTACC
GGGCGATTATAACTTGCAGTTTCTTGACCATGTGATTGACCACCCGACCTTGCGTTGGGTGGGATGCGCCAATGAGCTGA
ACGCCGCTTATACCGCGGACGGTTATGCGCGCATGTCGGGCGCTGGAGCGCTACTCACCACCTTTGGCGTGGGAGAACTT
AGCGCTATTAACGGTATCGCGGGCAGTTACGCGGAATATGTCCCGGTCTTGCATATCGTCGGCGCGCCCTGTAGCGCTGC
GCAGCAGCGTGGCGAATTGATGCACCATACCCTCGGTGACGGCGATTTTCGTCATTTTTATCGCATGAGTCAGGCGATAT
CCGCTGCCAGCGCAATATTGGATGAACAGAACGCCTGTTTCGAGATTGACCGCGTATTGGGTGAAATGCTTGCCGCACGC
AGGCCAGGATACATCATGCTGCCCGCCGACGTGGCGAAAAAAACGGCTATCCCGCCTACGGAGGCGCTGGCGTTGCCCGT
GCATGAAGCGCAAAGCGGCGTGGAGACGGCTTTTCGTTACCACGCCCGTCAGTGCCTGATGAACAGTCGGCGCATTGCGC
TATTGGCCGACTTTCTTGCCGGGCGTTTTGGTTTACGACCACTGTTGCAGCGCTGGATGGCGGAAACGCCCATCGCTCAT
GCGACACTACTGATGGGGAAGGGGCTTTTTGATGAACAGCACCCGAACTTCGTTGGCACCTATAGTGCAGGCGCCAGCAG
CAAAGAAGTACGTCAGGCCATAGAGGACGCCGATAGGGTTATCTGCGTCGGCACCCGTTTTGTCGATACCCTTACGGCCG
GATTTACCCAACAATTGCCGGCGGAACGCACGCTGGAGATTCAGCCTTACGCGTCGCGCATCGGCGAAACCTGGTTCAAC
CTCCCGATGGCGCAGGCAGTGTCTACGCTGCGCGAACTGTGCCTTGAATGCGCTTTTGCGCCGCCGCCGACGCGTTCCGC
CGGACAGCCAGTGCGGATTGATAAGGGAGAACTGACCCAGGAAAGCTTCTGGCAAACCTTACAGCAGTGTCTCAAACCCG
GCGATATTATCCTTGTCGACCAGGGGACCGCCGCCTTTGGCGCTGCCGCGCTGTCGCTTCCTGACGGCGCGGAAGTTGTG
GTTCAGCCGCTGTGGGGGTCTATCGGCTATTCCTTGCCCGCCGCGTTTGGCGCGCAAACCGCCTGCCCCGATCGGCGGGT
AATTCTGATTATTGGCGATGGCGCGGCGCAGCTCACGATTCAGGAGATGGGCTCGATGTTACGCGACGGGCAGGCGCCGG
TCATCCTGCTGCTCAACAATGACGGCTATACCGTAGAGCGCGCCATTCACGGCGCGGCCCAGCGGTATAACGACATCGCG
AGCTGGAACTGGACGCAGATACCACCGGCGCTAAACGCGGCGCAACAGGCGGAGTGCTGGCGGGTGACGCAGGCTATCCA
ACTGGCAGAGGTCCTCGAACGGTTGGCGCGCCCACAACGTCTGTCATTTATTGAAGTGATGTTGCCAAAAGCCGATCTGC
CGGAATTACTGCGTACCGTGACCCGGGCGCTGGAAGCCCGCAACGGGGGATAA

Upstream 100 bases:

>100_bases
CTGTAAACATTGTTTTCCAGGCTTTCATCCCCGCCGTGCTGGACAGCCATCGGCATTCCTTAATACTCAACATAATGTCA
ACGTCAGAAGGAAAGCTGTC

Downstream 100 bases:

>100_bases
TGGCCCCCGCTGCGCCGGATTAGGGTTCGTGACGGTTGGCGGCCAGCAACGGTTTTCCCGCCAGCAATAGCCAGGCGGGG
AGCATCACAATGCAGAGCAG

Product: decarboxylase

Products: NA

Alternate protein names: Indolepyruvate decarboxylase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MQNPYTVADYLLDRLAGCGIGHLFGVPGDYNLQFLDHVIDHPTLRWVGCANELNAAYTADGYARMSGAGALLTTFGVGEL
SAINGIAGSYAEYVPVLHIVGAPCSAAQQRGELMHHTLGDGDFRHFYRMSQAISAASAILDEQNACFEIDRVLGEMLAAR
RPGYIMLPADVAKKTAIPPTEALALPVHEAQSGVETAFRYHARQCLMNSRRIALLADFLAGRFGLRPLLQRWMAETPIAH
ATLLMGKGLFDEQHPNFVGTYSAGASSKEVRQAIEDADRVICVGTRFVDTLTAGFTQQLPAERTLEIQPYASRIGETWFN
LPMAQAVSTLRELCLECAFAPPPTRSAGQPVRIDKGELTQESFWQTLQQCLKPGDIILVDQGTAAFGAAALSLPDGAEVV
VQPLWGSIGYSLPAAFGAQTACPDRRVILIIGDGAAQLTIQEMGSMLRDGQAPVILLLNNDGYTVERAIHGAAQRYNDIA
SWNWTQIPPALNAAQQAECWRVTQAIQLAEVLERLARPQRLSFIEVMLPKADLPELLRTVTRALEARNGG

Sequences:

>Translated_550_residues
MQNPYTVADYLLDRLAGCGIGHLFGVPGDYNLQFLDHVIDHPTLRWVGCANELNAAYTADGYARMSGAGALLTTFGVGEL
SAINGIAGSYAEYVPVLHIVGAPCSAAQQRGELMHHTLGDGDFRHFYRMSQAISAASAILDEQNACFEIDRVLGEMLAAR
RPGYIMLPADVAKKTAIPPTEALALPVHEAQSGVETAFRYHARQCLMNSRRIALLADFLAGRFGLRPLLQRWMAETPIAH
ATLLMGKGLFDEQHPNFVGTYSAGASSKEVRQAIEDADRVICVGTRFVDTLTAGFTQQLPAERTLEIQPYASRIGETWFN
LPMAQAVSTLRELCLECAFAPPPTRSAGQPVRIDKGELTQESFWQTLQQCLKPGDIILVDQGTAAFGAAALSLPDGAEVV
VQPLWGSIGYSLPAAFGAQTACPDRRVILIIGDGAAQLTIQEMGSMLRDGQAPVILLLNNDGYTVERAIHGAAQRYNDIA
SWNWTQIPPALNAAQQAECWRVTQAIQLAEVLERLARPQRLSFIEVMLPKADLPELLRTVTRALEARNGG
>Mature_550_residues
MQNPYTVADYLLDRLAGCGIGHLFGVPGDYNLQFLDHVIDHPTLRWVGCANELNAAYTADGYARMSGAGALLTTFGVGEL
SAINGIAGSYAEYVPVLHIVGAPCSAAQQRGELMHHTLGDGDFRHFYRMSQAISAASAILDEQNACFEIDRVLGEMLAAR
RPGYIMLPADVAKKTAIPPTEALALPVHEAQSGVETAFRYHARQCLMNSRRIALLADFLAGRFGLRPLLQRWMAETPIAH
ATLLMGKGLFDEQHPNFVGTYSAGASSKEVRQAIEDADRVICVGTRFVDTLTAGFTQQLPAERTLEIQPYASRIGETWFN
LPMAQAVSTLRELCLECAFAPPPTRSAGQPVRIDKGELTQESFWQTLQQCLKPGDIILVDQGTAAFGAAALSLPDGAEVV
VQPLWGSIGYSLPAAFGAQTACPDRRVILIIGDGAAQLTIQEMGSMLRDGQAPVILLLNNDGYTVERAIHGAAQRYNDIA
SWNWTQIPPALNAAQQAECWRVTQAIQLAEVLERLARPQRLSFIEVMLPKADLPELLRTVTRALEARNGG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG3961

COG function: function code GHR; Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=490, Percent_Identity=23.6734693877551, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=547, Percent_Identity=37.6599634369287, Blast_Score=346, Evalue=6e-96,
Organism=Saccharomyces cerevisiae, GI6323073, Length=563, Percent_Identity=37.1225577264654, Blast_Score=339, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6323163, Length=552, Percent_Identity=37.3188405797101, Blast_Score=337, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6320123, Length=558, Percent_Identity=31.8996415770609, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6320588, Length=604, Percent_Identity=30.2980132450331, Blast_Score=276, Evalue=9e-75,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017764
- InterPro:   IPR012110
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.74 [H]

Molecular weight: Translated: 59750; Mature: 59750

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00626 RCC1_2 ; PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPYTVADYLLDRLAGCGIGHLFGVPGDYNLQFLDHVIDHPTLRWVGCANELNAAYTAD
CCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHCCCCEEEEECHHHCCCCEECC
GYARMSGAGALLTTFGVGELSAINGIAGSYAEYVPVLHIVGAPCSAAQQRGELMHHTLGD
CHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
GDFRHFYRMSQAISAASAILDEQNACFEIDRVLGEMLAARRPGYIMLPADVAKKTAIPPT
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCC
EALALPVHEAQSGVETAFRYHARQCLMNSRRIALLADFLAGRFGLRPLLQRWMAETPIAH
HHEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHH
ATLLMGKGLFDEQHPNFVGTYSAGASSKEVRQAIEDADRVICVGTRFVDTLTAGFTQQLP
HHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCC
AERTLEIQPYASRIGETWFNLPMAQAVSTLRELCLECAFAPPPTRSAGQPVRIDKGELTQ
CCCEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCH
ESFWQTLQQCLKPGDIILVDQGTAAFGAAALSLPDGAEVVVQPLWGSIGYSLPAAFGAQT
HHHHHHHHHHCCCCCEEEECCCCHHHHHHEECCCCCHHHHHHHHHHHCCCCCHHHHCCCC
ACPDRRVILIIGDGAAQLTIQEMGSMLRDGQAPVILLLNNDGYTVERAIHGAAQRYNDIA
CCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
SWNWTQIPPALNAAQQAECWRVTQAIQLAEVLERLARPQRLSFIEVMLPKADLPELLRTV
CCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
TRALEARNGG
HHHHHCCCCC
>Mature Secondary Structure
MQNPYTVADYLLDRLAGCGIGHLFGVPGDYNLQFLDHVIDHPTLRWVGCANELNAAYTAD
CCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHCCCCEEEEECHHHCCCCEECC
GYARMSGAGALLTTFGVGELSAINGIAGSYAEYVPVLHIVGAPCSAAQQRGELMHHTLGD
CHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
GDFRHFYRMSQAISAASAILDEQNACFEIDRVLGEMLAARRPGYIMLPADVAKKTAIPPT
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCC
EALALPVHEAQSGVETAFRYHARQCLMNSRRIALLADFLAGRFGLRPLLQRWMAETPIAH
HHEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHH
ATLLMGKGLFDEQHPNFVGTYSAGASSKEVRQAIEDADRVICVGTRFVDTLTAGFTQQLP
HHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCC
AERTLEIQPYASRIGETWFNLPMAQAVSTLRELCLECAFAPPPTRSAGQPVRIDKGELTQ
CCCEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCH
ESFWQTLQQCLKPGDIILVDQGTAAFGAAALSLPDGAEVVVQPLWGSIGYSLPAAFGAQT
HHHHHHHHHHCCCCCEEEECCCCHHHHHHEECCCCCHHHHHHHHHHHCCCCCHHHHCCCC
ACPDRRVILIIGDGAAQLTIQEMGSMLRDGQAPVILLLNNDGYTVERAIHGAAQRYNDIA
CCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
SWNWTQIPPALNAAQQAECWRVTQAIQLAEVLERLARPQRLSFIEVMLPKADLPELLRTV
CCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
TRALEARNGG
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034209 [H]