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The map label for this gene is narQ [H]

Identifier: 29140906

GI number: 29140906

Start: 451146

End: 452846

Strand: Reverse

Name: narQ [H]

Synonym: t0379

Alternate gene names: 29140906

Gene position: 452846-451146 (Counterclockwise)

Preceding gene: 29140908

Following gene: 29140905

Centisome position: 9.45

GC content: 53.73

Gene sequence:

>1701_bases
GTGACGGTTAAACGCCCGGTCTCGGCCAGTCTGGCTAAGGCTTTTTTCTATATTGTGCTGTTGTCTATTCTGTCCACCGG
CAGCGCATTACTGACGCTGACCAGCAGTTTACGGGATGCCGAAGCGATCAATATCGCCGGTTCGCTACGAATGCAGAGCT
ATCGGCTGGGTTACGATCTGCAAAGCCGTAGCCCGCAGATTAATGCGCATCGTCAACTTTTCCAGCACGCGCTCAACTCG
CCGGTACTGCAAAATCTCAACGCCTGGTATGTGCCGCAGGCGGTGAAAACTCGCTACGCGCGCCTGCACGCCAACTGGCT
GGAGATGAATAGCCGCCTACAGGATGGCGACATTGTCTGGTATCAGTCCAATATCAACAATTACGTCGATCAAATTGATC
TCTTCGTTCTGGCGTTACAGCATTACGCCGAGCGTAAAGTCATGCTGGTCGTCGCCATATCGCTGGCGGGCGGTATCGGC
ATATTTACGCTGGTCTTTTTTACCCTACGGCGTATTCGCCAGCAGGTCGTTCGCCCGCTGAATCAACTGGTCACCGCCAG
TCAGCGAATTGAACATGGTCAGTTCGCCCCGCTGCCGCTGGATACCAGCTTACCCAATGAGCTGGGATTGTTGGCGAAAA
CGTTTAGTCAGATGTCGAGCGAGCTGCATAAACTTTATCGTTCGCTGGAAGCCTCGGTGGAAGAAAAAACGCACGATCTG
CACGAAGCGCACCGCCGCCTGGAGGTGTTATACCAGTGCTCCCAGGCGCTGAATACCAGCCAAATCGATGTCCACTGTTT
TCGCCATATTTTGCAAATTGTCCGTGAACATGACGCCGCCTGGTATCTGGAGTTAACCGTGGGCGATAACTGGCGGATTA
GCGAAGGCACGCAAAGCCCCGACCTGCCGATGCAAATGCTGCCAGTGACGATGCAGGACACGGTCTATGGCGAACTCCAC
TGGCAAAGCCCCAACGTTAATGCCTCTACGCCGTTGCTGAACAGCGTCTCCACGATGCTGGGGCGTGGTTTGTACTTTAA
TCAGGCGCAAAAACACTTCCAGCAACTTTTGTTAATGGAAGAGCGCGCGACGATTGCCCGTGAGTTACACGATTCGCTGG
CGCAGGTACTTTCTTATTTACGTATTCAATTAACGCTTTTGAAGCGCGCCATTCCAGAAGATAACGCCGGCGCGCAAAGC
ATCATGGCGGATTTTTCCAGAGCGCTGAACGACGCCTACCGTCAGCTTCGCGAGCTACTCACCACGTTCCGTCTGACGTT
ACAGCAGGCCGATTTACCCTCCGCATTGCATGAGATGCTGGAAGATTTACAGAGTCAGACTCCGGCTAAACTCAAGTTGG
ATTGCCGTTTACCCACGCTGGCGCTGGATGCGCAAATGCAGGTGCATTTATTGCAAATTGTGCGTGAAGCTGTCCTGAAC
GCCATTAAACACGCCAACGCGAGCGAAATCGCCGTGAGCTGCGTCACCGCGCCTGACGGCGATCATACCGTTTATATTCG
CGATAATGGCATTGGTATCGGCGAACCGCATGAGCCTGCCGGACACTATGGTCTGAATATAATGCGTGAGCGCGCGGAAC
GGCTTGGCGGCACGCTGAATTTTTCGCAACCCTCCGGCGGCGGCACGCTGGTCAGTATCAGTTTTCGCTCCTCCAACAAG
GAAGAGAGTCAGTTAACGTAA

Upstream 100 bases:

>100_bases
TCTGCCCAGGAAGCACAACTATCTGTCCAGAACCTGTGGCAGATCAATTTATTTCAAAGCAAATGAAATTGCGGTTCAGC
TAAATGCGGGGAGTACCAGT

Downstream 100 bases:

>100_bases
AGAACGGCAAATCACCGGAAGGCCAGATGTCAGGCTTTCCGGCGTTGGCACAAAAAGTATGCTGTAAAAGCGCATGATAA
TTTACATTATCTCCTTTATT

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTVKRPVSASLAKAFFYIVLLSILSTGSALLTLTSSLRDAEAINIAGSLRMQSYRLGYDLQSRSPQINAHRQLFQHALNS
PVLQNLNAWYVPQAVKTRYARLHANWLEMNSRLQDGDIVWYQSNINNYVDQIDLFVLALQHYAERKVMLVVAISLAGGIG
IFTLVFFTLRRIRQQVVRPLNQLVTASQRIEHGQFAPLPLDTSLPNELGLLAKTFSQMSSELHKLYRSLEASVEEKTHDL
HEAHRRLEVLYQCSQALNTSQIDVHCFRHILQIVREHDAAWYLELTVGDNWRISEGTQSPDLPMQMLPVTMQDTVYGELH
WQSPNVNASTPLLNSVSTMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRAIPEDNAGAQS
IMADFSRALNDAYRQLRELLTTFRLTLQQADLPSALHEMLEDLQSQTPAKLKLDCRLPTLALDAQMQVHLLQIVREAVLN
AIKHANASEIAVSCVTAPDGDHTVYIRDNGIGIGEPHEPAGHYGLNIMRERAERLGGTLNFSQPSGGGTLVSISFRSSNK
EESQLT

Sequences:

>Translated_566_residues
MTVKRPVSASLAKAFFYIVLLSILSTGSALLTLTSSLRDAEAINIAGSLRMQSYRLGYDLQSRSPQINAHRQLFQHALNS
PVLQNLNAWYVPQAVKTRYARLHANWLEMNSRLQDGDIVWYQSNINNYVDQIDLFVLALQHYAERKVMLVVAISLAGGIG
IFTLVFFTLRRIRQQVVRPLNQLVTASQRIEHGQFAPLPLDTSLPNELGLLAKTFSQMSSELHKLYRSLEASVEEKTHDL
HEAHRRLEVLYQCSQALNTSQIDVHCFRHILQIVREHDAAWYLELTVGDNWRISEGTQSPDLPMQMLPVTMQDTVYGELH
WQSPNVNASTPLLNSVSTMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRAIPEDNAGAQS
IMADFSRALNDAYRQLRELLTTFRLTLQQADLPSALHEMLEDLQSQTPAKLKLDCRLPTLALDAQMQVHLLQIVREAVLN
AIKHANASEIAVSCVTAPDGDHTVYIRDNGIGIGEPHEPAGHYGLNIMRERAERLGGTLNFSQPSGGGTLVSISFRSSNK
EESQLT
>Mature_565_residues
TVKRPVSASLAKAFFYIVLLSILSTGSALLTLTSSLRDAEAINIAGSLRMQSYRLGYDLQSRSPQINAHRQLFQHALNSP
VLQNLNAWYVPQAVKTRYARLHANWLEMNSRLQDGDIVWYQSNINNYVDQIDLFVLALQHYAERKVMLVVAISLAGGIGI
FTLVFFTLRRIRQQVVRPLNQLVTASQRIEHGQFAPLPLDTSLPNELGLLAKTFSQMSSELHKLYRSLEASVEEKTHDLH
EAHRRLEVLYQCSQALNTSQIDVHCFRHILQIVREHDAAWYLELTVGDNWRISEGTQSPDLPMQMLPVTMQDTVYGELHW
QSPNVNASTPLLNSVSTMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRAIPEDNAGAQSI
MADFSRALNDAYRQLRELLTTFRLTLQQADLPSALHEMLEDLQSQTPAKLKLDCRLPTLALDAQMQVHLLQIVREAVLNA
IKHANASEIAVSCVTAPDGDHTVYIRDNGIGIGEPHEPAGHYGLNIMRERAERLGGTLNFSQPSGGGTLVSISFRSSNKE
ESQLT

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=565, Percent_Identity=87.9646017699115, Blast_Score=1019, Evalue=0.0,
Organism=Escherichia coli, GI1787474, Length=602, Percent_Identity=29.734219269103, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87082311, Length=210, Percent_Identity=32.8571428571429, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63726; Mature: 63595

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKRPVSASLAKAFFYIVLLSILSTGSALLTLTSSLRDAEAINIAGSLRMQSYRLGYDL
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
QSRSPQINAHRQLFQHALNSPVLQNLNAWYVPQAVKTRYARLHANWLEMNSRLQDGDIVW
CCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YQSNINNYVDQIDLFVLALQHYAERKVMLVVAISLAGGIGIFTLVFFTLRRIRQQVVRPL
EECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
NQLVTASQRIEHGQFAPLPLDTSLPNELGLLAKTFSQMSSELHKLYRSLEASVEEKTHDL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEAHRRLEVLYQCSQALNTSQIDVHCFRHILQIVREHDAAWYLELTVGDNWRISEGTQSP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEECCCCCCC
DLPMQMLPVTMQDTVYGELHWQSPNVNASTPLLNSVSTMLGRGLYFNQAQKHFQQLLLME
CCCHHHCCCCHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERATIARELHDSLAQVLSYLRIQLTLLKRAIPEDNAGAQSIMADFSRALNDAYRQLRELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TTFRLTLQQADLPSALHEMLEDLQSQTPAKLKLDCRLPTLALDAQMQVHLLQIVREAVLN
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHH
AIKHANASEIAVSCVTAPDGDHTVYIRDNGIGIGEPHEPAGHYGLNIMRERAERLGGTLN
HHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
FSQPSGGGTLVSISFRSSNKEESQLT
CCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TVKRPVSASLAKAFFYIVLLSILSTGSALLTLTSSLRDAEAINIAGSLRMQSYRLGYDL
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
QSRSPQINAHRQLFQHALNSPVLQNLNAWYVPQAVKTRYARLHANWLEMNSRLQDGDIVW
CCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YQSNINNYVDQIDLFVLALQHYAERKVMLVVAISLAGGIGIFTLVFFTLRRIRQQVVRPL
EECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
NQLVTASQRIEHGQFAPLPLDTSLPNELGLLAKTFSQMSSELHKLYRSLEASVEEKTHDL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEAHRRLEVLYQCSQALNTSQIDVHCFRHILQIVREHDAAWYLELTVGDNWRISEGTQSP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEECCCCCCC
DLPMQMLPVTMQDTVYGELHWQSPNVNASTPLLNSVSTMLGRGLYFNQAQKHFQQLLLME
CCCHHHCCCCHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERATIARELHDSLAQVLSYLRIQLTLLKRAIPEDNAGAQSIMADFSRALNDAYRQLRELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TTFRLTLQQADLPSALHEMLEDLQSQTPAKLKLDCRLPTLALDAQMQVHLLQIVREAVLN
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHH
AIKHANASEIAVSCVTAPDGDHTVYIRDNGIGIGEPHEPAGHYGLNIMRERAERLGGTLN
HHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
FSQPSGGGTLVSISFRSSNKEESQLT
CCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]