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The map label for this gene is hda [H]

Identifier: 29140889

GI number: 29140889

Start: 430548

End: 431273

Strand: Direct

Name: hda [H]

Synonym: t0361

Alternate gene names: 29140889

Gene position: 430548-431273 (Clockwise)

Preceding gene: 29140888

Following gene: 29140892

Centisome position: 8.98

GC content: 53.72

Gene sequence:

>726_bases
ATGTCATCCTGGGTTGAGGTATCTCTGAACACACCGGCACAGCTCTCTTTGCCACTTTATCTTCCTGACGACGAAACTTT
CGCAAGTTTCTGGCCGGGGGATAACGCCTCTCTACTGGCCGCGTTACAAAACGTGTTGCGCCAGGAACATAGTGGATATA
TCTACCTTTGGGCGCGTGAAGGCGCGGGCCGCAGCCATTTGCTGCACGCCGCCTGCGCTGAACTGTCGCAGCGTGGAGAT
GCGGTAGGCTACGTCCCGCTCGATAAACGCACCTGGTTCGTGCCGGAAGTGCTCGACGGTATGGAACACCTCTCGCTGGT
GTGTATTGATAACATTGAGTGCGTCGCTGGCGATGAACTGTGGGAGATGGCCATCTTTGATCTCTATAACCGCATTCTGG
AGTCCGGTAAGACGCGGTTATTGATCACCGGCGATCGTCCGCCAAGACAGTTAAATCTGGGCTTACCCGATCTCGCTTCC
CGTCTGGACTGGGGGCAAATCTATAAACTCCAGCCTCTTTCCGATGAAGACAAACTTCAGGCGCTACAGTTGCGTGCCCG
ATTACGGGGGTTTGAACTGCCGGAAGATGTCGGACGGTTTTTACTCAAACGTCTTGATCGCGAAATGCGCACGCTGTTTA
TGACGCTGGATCAGCTCGATCATGCGTCGATCACTGCCCAGCGAAAACTGACGATCCCGTTTGTCAAAGAGATCCTGAAA
CTGTAA

Upstream 100 bases:

>100_bases
GCAAATGATGCGGAGTCTCCGCATCAGTAACGGGTTGCCGGGCAGCGATGCTGCCCGGTTCTATCTCACGGGAATTATGT
GGTAAACTCAGCGCGATTTT

Downstream 100 bases:

>100_bases
TACGCCGCGACGGCGGCGTTTAACGGTTAACCGAGGATGTCCAGCACCTGCTCAGGCGGTCGGCCAATACGCGCCTGACC
ATTTGCCACTACAATGGGGC

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSSWVEVSLNTPAQLSLPLYLPDDETFASFWPGDNASLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGD
AVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECVAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLAS
RLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILK
L

Sequences:

>Translated_241_residues
MSSWVEVSLNTPAQLSLPLYLPDDETFASFWPGDNASLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGD
AVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECVAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLAS
RLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILK
L
>Mature_240_residues
SSWVEVSLNTPAQLSLPLYLPDDETFASFWPGDNASLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDA
VGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECVAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASR
LDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILKL

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=233, Percent_Identity=98.2832618025751, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI2367267, Length=255, Percent_Identity=27.0588235294118, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 27472; Mature: 27341

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSWVEVSLNTPAQLSLPLYLPDDETFASFWPGDNASLLAALQNVLRQEHSGYIYLWARE
CCCEEEEEECCCCEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
GAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECVAGDEL
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHEEEEEECCCEEECCHHH
WEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDEDKLQ
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCHHHHH
ALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHC
L
C
>Mature Secondary Structure 
SSWVEVSLNTPAQLSLPLYLPDDETFASFWPGDNASLLAALQNVLRQEHSGYIYLWARE
CCEEEEEECCCCEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
GAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECVAGDEL
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHEEEEEECCCEEECCHHH
WEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDEDKLQ
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCHHHHH
ALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA