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The map label for this gene is uraA [H]

Identifier: 29140888

GI number: 29140888

Start: 429188

End: 430477

Strand: Direct

Name: uraA [H]

Synonym: t0360

Alternate gene names: 29140888

Gene position: 429188-430477 (Clockwise)

Preceding gene: 29140887

Following gene: 29140889

Centisome position: 8.96

GC content: 55.97

Gene sequence:

>1290_bases
ATGACGCGCCGTGCTATCGGGGTGAGTGAAAGACCGCCGCTTTTACAGACAATCCCGCTTAGTTTACAGCACCTTTTCGC
CATGTTTGGCGCGACCGTGCTGGTGCCAGTTCTGTTTCATATCAATCCCGCGACGGTGCTGCTGTTTAACGGTATCGGAA
CGTTGCTGTATCTCTTTATCTGCAAAGGTAAAATTCCTGCCTACCTCGGATCGAGCTTTGCCTTTATTTCCCCGGTATTA
CTGTTGTTGCCGCTGGGTTATGAAGTGGCGCTGGGCGGTTTTATTATGTGCGGCGTGTTGTTCTGTCTGGTCTCTTTCAT
CGTTAAAAAAGCGGGCACCGGCTGGCTGGATGTGATGTTCCCGCCTGCGGCAATGGGCGCAATCGTTGCCGTCATCGGTC
TGGAGCTGGCTGGCGTCGCGGCGGGGATGGCCGGATTGCTGCCTGCGCAAGGGCAGTCGCCGGACACGAAAACAATTATT
ATCTCCATGGTCACGCTGGCGGTGACGGTGTTCGGCTCCGTACTGTTTCGCGGTTTCCTGGCGATCATTCCGATTTTGAT
CGGCGTGCTGGCGGGCTATGCGCTGTCATTCGCGCTGGGGGTGGTCGATACCACGCCGATTGCCCAGGCGCACTGGTTTG
CGCTGCCGACCTTCTATACGCCGCGTTTTGAATGGTTTGCGATCCTGACGATTCTGCCCGCGGCGTTGGTCGTGATCGCC
GAGCATGTCGGTCATCTGGTGGTGACGGCGAATATCGTCAAAAAAGATTTAGTGCGCGATCCCGGTTTGCACCGCTCGAT
GTTCGCTAACGGACTGTCGACGATCATTTCCGGTTTCTTCGGCTCCACGCCGAATACCACCTATGGGGAAAATATTGGCG
TCATGGCGATCACCCGCGTTTACAGTACCTGGGTTATCGGCGGCGCGGCGATTTTCGCCATTCTGCTTTCCTGCGTCGGC
AAACTGGCGGCGGCGATTCAGATTATCCCGTTACCCGTGATGGGCGGCGTCTCGCTGCTGTTGTACGGCGTCATCGGCGC
GTCGGGGATTCGCGTCTTGATCGAATCGAAAGTCGACTACAACAAAGCGCAAAACCTGATCCTCACCTCGGTGATTTTGA
TCATCGGCGTTAGCGGCGCGAAAGTGCATATCGGCGCGGCAGAATTGAAAGGGATGGCGCTGGCGACCATCGTCGGGATT
GGCCTGAGCCTGATTTTTAAACTGATTAGCCTGTTGCGTCCGGAAGAAGTGGTACTGGAGGCAAATGATGCGGAGTCTCC
GCATCAGTAA

Upstream 100 bases:

>100_bases
TGGTACCAAATAAGTGAATAAATAATTAAAAGCCGACTTTAAGAGTCGGCTTTTTTTTGAATAAAACCACTAATAACAAA
CACACTTAGAGGAAAACACT

Downstream 100 bases:

>100_bases
CGGGTTGCCGGGCAGCGATGCTGCCCGGTTCTATCTCACGGGAATTATGTGGTAAACTCAGCGCGATTTTATGTCATCCT
GGGTTGAGGTATCTCTGAAC

Product: uracil transporter

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVL
LLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMFPPAAMGAIVAVIGLELAGVAAGMAGLLPAQGQSPDTKTII
ISMVTLAVTVFGSVLFRGFLAIIPILIGVLAGYALSFALGVVDTTPIAQAHWFALPTFYTPRFEWFAILTILPAALVVIA
EHVGHLVVTANIVKKDLVRDPGLHRSMFANGLSTIISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVG
KLAAAIQIIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGI
GLSLIFKLISLLRPEEVVLEANDAESPHQ

Sequences:

>Translated_429_residues
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVL
LLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMFPPAAMGAIVAVIGLELAGVAAGMAGLLPAQGQSPDTKTII
ISMVTLAVTVFGSVLFRGFLAIIPILIGVLAGYALSFALGVVDTTPIAQAHWFALPTFYTPRFEWFAILTILPAALVVIA
EHVGHLVVTANIVKKDLVRDPGLHRSMFANGLSTIISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVG
KLAAAIQIIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGI
GLSLIFKLISLLRPEEVVLEANDAESPHQ
>Mature_428_residues
TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLL
LLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMFPPAAMGAIVAVIGLELAGVAAGMAGLLPAQGQSPDTKTIII
SMVTLAVTVFGSVLFRGFLAIIPILIGVLAGYALSFALGVVDTTPIAQAHWFALPTFYTPRFEWFAILTILPAALVVIAE
HVGHLVVTANIVKKDLVRDPGLHRSMFANGLSTIISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGK
LAAAIQIIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGIG
LSLIFKLISLLRPEEVVLEANDAESPHQ

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI40316845, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI44680143, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI44680145, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788843, Length=425, Percent_Identity=95.0588235294118, Blast_Score=798, Evalue=0.0,
Organism=Escherichia coli, GI87081818, Length=425, Percent_Identity=36.2352941176471, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI87082181, Length=429, Percent_Identity=26.5734265734266, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790087, Length=444, Percent_Identity=25.9009009009009, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17541904, Length=233, Percent_Identity=26.1802575107296, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558856, Length=173, Percent_Identity=30.0578034682081, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17542262, Length=185, Percent_Identity=29.1891891891892, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356175, Length=435, Percent_Identity=23.9080459770115, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45098; Mature: 44967

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCC
>Mature Secondary Structure 
TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHH
CKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMF
HCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PPAAMGAIVAVIGLELAGVAAGMAGLLPAQGQSPDTKTIIISMVTLAVTVFGSVLFRGFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AIIPILIGVLAGYALSFALGVVDTTPIAQAHWFALPTFYTPRFEWFAILTILPAALVVIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHH
EHVGHLVVTANIVKKDLVRDPGLHRSMFANGLSTIISGFFGSTPNTTYGENIGVMAITRV
HHHCHHEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
YSTWVIGGAAIFAILLSCVGKLAAAIQIIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCH
NKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGIGLSLIFKLISLLRPEEVVLE
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ANDAESPHQ
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]