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The map label for this gene is hmpA [H]

Identifier: 29140834

GI number: 29140834

Start: 344300

End: 345490

Strand: Reverse

Name: hmpA [H]

Synonym: t0300

Alternate gene names: 29140834

Gene position: 345490-344300 (Counterclockwise)

Preceding gene: 29140837

Following gene: 29140833

Centisome position: 7.21

GC content: 56.09

Gene sequence:

>1191_bases
ATGCTTGACGCACAAACCATCGCTACAGTAAAGGCCACCATTCCCCTGCTGGTTGAAACAGGACCGAAACTGACCGCCCA
CTTCTACGACCGTATGTTTACGCATAACCCGGAGCTCAAAGAAATCTTCAATATGAGCAACCAGCGTAACGGTGATCAGC
GCGAAGCCCTGTTTAATGCTATCGCGGCCTACGCCAGCAATATCGAAAATTTACCGGCGTTGCTGCCGGCGGTAGAAAAA
ATCGCGCAGAAGCACACCAGCTTCCAGATTAAGCCGGAGCAGTACAACATCGTCGGGACGCATCTGCTGGCGACGCTGGA
TGAAATGTTCAACCCGGGCCAGGAAGTGCTGGACGCATGGGGTAAAGCCTATGGCGTACTGGCTAACGTCTTTATTCATC
GGGAAGCCGAGATTTATCACGAGAACGCCAGCAAAGACGGCGGCTGGGAAGGCACGCGCCCCTTCCGCATCGTTGCGAAA
ACCCCGCGTAGCGCACTGATCACCAGCTTTGAGTTTGAACCGGTCGACGGCGGTACGGTGGCGGAATACCGTCCCGGGCA
GTATCTGGGCGTCTGGCTGAAGCCGGAAGGTTTTGCGCATCAGGAGATCCGCCAATATTCACTGACCCGTAAACCCGATG
GCAAAGGGTACCGTATTGCCGTGAAGCGTGAAGACGGCGGCCAGGTATCAAACTGGTTACACCATCACGCCAACGTAGGC
GATGTGGTGCATCTGGCTGCGCCGGCAGGTGACTTCTTTATGAATGTCGCCGCTGATACCCCCGTTTCGCTGATTTCCGC
TGGCGTCGGCCAGACGCCGATGTTGGCGATGCTCGATACGCTGGCGAAAGAACAGCATACCGCGCAGGTGAACTGGTTCC
ACGCGGCGGAGAACGGCGACGTCCATGCGTTTGCCGACGAAGTGAGCGAGCTGGGCCGTACACTGCCGCGTTTCACTGCC
CATACCTGGTACCGCGAGCCAACCGAGGCCGATCGCGCCCAACGCGTCTTTGACAGCGAAGGGCTGATGGATTTAAGCAA
ACTGGAAGCCGCGATCAGCGATCCGGCAATGCAGTTCTATCTTTGCGGCCCGGTAGGCTTTATGCAGTTTGCCGCAAAAC
AACTGGTTTCACTTGGCGTGAATAACGAAAACATTCATTACGAATGCTTCGGCCCGCATAAAGTGCTGTAA

Upstream 100 bases:

>100_bases
TCCATAACGTAAAGCAGAGAAGATCCATTTACAATGCAAGGGTATTTTTATAAGATGCATTTGATATACATTATTAGATT
TTCACATAAAGGAAGCACGT

Downstream 100 bases:

>100_bases
TTGCAAGAAATCCGCTGAAAAAACGGGCGTTATCGTTAAGGCGAACGCCCGTTTTCCTCCCCGTTCTCCTGCTTATTGCG
CTGCTTTTTATGCCTTTCTT

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEK
IAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAWGKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAK
TPRSALITSFEFEPVDGGTVAEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHANVG
DVVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGDVHAFADEVSELGRTLPRFTA
HTWYREPTEADRAQRVFDSEGLMDLSKLEAAISDPAMQFYLCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL

Sequences:

>Translated_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEK
IAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAWGKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAK
TPRSALITSFEFEPVDGGTVAEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHANVG
DVVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGDVHAFADEVSELGRTLPRFTA
HTWYREPTEADRAQRVFDSEGLMDLSKLEAAISDPAMQFYLCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL
>Mature_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEK
IAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAWGKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAK
TPRSALITSFEFEPVDGGTVAEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHANVG
DVVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGDVHAFADEVSELGRTLPRFTA
HTWYREPTEADRAQRVFDSEGLMDLSKLEAAISDPAMQFYLCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=91.6666666666667, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=215, Percent_Identity=26.9767441860465, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787098, Length=202, Percent_Identity=28.2178217821782, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=35.1219512195122, Blast_Score=210, Evalue=3e-55,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44031; Mature: 44031

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAW
HHHHHHCHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAKTPRSALITSFEFEPVDGGTV
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHEEEEEECCCCCCCC
AEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHANVG
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
DVVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGD
CEEEEECCCCHHHEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCC
VHAFADEVSELGRTLPRFTAHTWYREPTEADRAQRVFDSEGLMDLSKLEAAISDPAMQFY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEE
LCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAW
HHHHHHCHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAKTPRSALITSFEFEPVDGGTV
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHEEEEEECCCCCCCC
AEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHANVG
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
DVVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGD
CEEEEECCCCHHHEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCC
VHAFADEVSELGRTLPRFTAHTWYREPTEADRAQRVFDSEGLMDLSKLEAAISDPAMQFY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEE
LCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]