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The map label for this gene is yfhD [H]

Identifier: 29140824

GI number: 29140824

Start: 326938

End: 328365

Strand: Reverse

Name: yfhD [H]

Synonym: t0290

Alternate gene names: 29140824

Gene position: 328365-326938 (Counterclockwise)

Preceding gene: 29140830

Following gene: 29140819

Centisome position: 6.85

GC content: 52.31

Gene sequence:

>1428_bases
TTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATATTGACGCTGCTGCTGGCAGCAGCCCTTTGGCCCTCAATCCC
CTGGTTCGGTAAAACCGAAAACCATATCGCCGCGATTCAAGCCCGGGGGGTACTGCGCGTCAGTACGATCGACTCGCCAT
TAACCTATTCCGTTATCAACGGTAAAAAATATGGTCTGGACTATGAACTGGCGCAGCAGTTCGCCAACTATCTCGGCGTG
AAGCTGAAAGTGACGGTGCGCCAGAATATCAGCCAGTTGTTTGACGATCTGGATAACGGCAATGCCGACCTGCTCGCGGC
AGGGCTGGTCTACGACAGCGCGCGCGTTAAAAACTATCAGCCCGGCCCCATGTATTATTCGGTTTCGCAACAGCTCGTTT
ACCGGGTCGGACAATATCGCCCACGTTCGCTGGCGACCGTTAACGAGAATCAGCTTACCATTGCGCCAGGGCACGTCGTG
GTCAATGATTTACAGCGTTTAAAAGAGACAAAGTTTCCCGACCTGAGCTGGAAGGTGGATGATAAAAAAGGCTCGACGAC
GCTGCTGGAAGAGGTCATCAGCGGCAAACTGGATTACACCATTGCCGATTCAGTGGCGATCAGTCTGTTTCAGCGCGTCC
ATCCCGAACTGGCCGTGGCGCTGGATGTCACGGATGAACAGCCCGTGACCTGGTTTAGCCGACTGGACGACGATAATACG
CTCTCCGCCGCGCTGCTCGATTTTTTCAATTCGATCAATGAAGACGGGTCGTTGGCGCGGATCGAAGAGAAATATCTGGG
GCATGGCGATGATTTCGACTACGTTGATACCCGCTCTTTCCTGCGAGCGGTGGATAACGTCCTGCCGGAGCTGGAACCGT
TATTTAAAAAATATGCCAAAGAGATAGACTGGCGCCTGCTGGCGGCGATTTCTTATCAGGAATCACACTGGGACCCGCTG
GCGACCTCGCCAACCGGCGTGCGCGGCCTGATGATGCTGACTAAAAATACCGCCCAAAGTCTGGGGCTGACCGACCGTAC
CGATGCGGAGCAAAGCATCAGCGGCGGTGCTCGCTATCTTGAAGATATGATGGCTAAAGTTCCCGAAACCGTTCCGGAAG
ACGAGCGTATCTGGTTTGCCTTAGCCGCCTACAATATGGGTTATGCGCACATGCTCGATGCCAGATCGCTGACGGTAAAA
ACCAAAGGCAATCCGGATAGCTGGACCGATGTGAAGCAGCGTTTGCCTCTGTTAAGCCAAAAACCGTATTACAGTAAGCT
AACCTACGGTTACGCGCGTGGACATGAGGCTTATGCTTATGTGGAAAATATCCGTAAATACCAGATAAGTTTGGTGGGGT
ATTTGCAGGAAAAAGAAAAGCAGGAAGCGGAAGCTATGAAACTGGCGCAGGATTACCCGGCGGTGTAG

Upstream 100 bases:

>100_bases
GGCGCATTGTTACGCAAAATGCCGCTCATTAGTGACTTATCTATTGCAGCAAAACATCTTTTTCAGCCCGAGGCAACGCA
CGATCCAGAGAATTAACTAA

Downstream 100 bases:

>100_bases
CCGGAGGAGCTGAATAAAGCCCCCTTCCCCTTCCTGTCGTTTTTATCGCAGTCATCAGGCTACCTGACGCATTCGCCGTC
TTTACTGTTTACGCCGCAGA

Product: transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKKLKINYLFIGILTLLLAAALWPSIPWFGKTENHIAAIQARGVLRVSTIDSPLTYSVINGKKYGLDYELAQQFANYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYDSARVKNYQPGPMYYSVSQQLVYRVGQYRPRSLATVNENQLTIAPGHVV
VNDLQRLKETKFPDLSWKVDDKKGSTTLLEEVISGKLDYTIADSVAISLFQRVHPELAVALDVTDEQPVTWFSRLDDDNT
LSAALLDFFNSINEDGSLARIEEKYLGHGDDFDYVDTRSFLRAVDNVLPELEPLFKKYAKEIDWRLLAAISYQESHWDPL
ATSPTGVRGLMMLTKNTAQSLGLTDRTDAEQSISGGARYLEDMMAKVPETVPEDERIWFALAAYNMGYAHMLDARSLTVK
TKGNPDSWTDVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQEAEAMKLAQDYPAV

Sequences:

>Translated_475_residues
MKKLKINYLFIGILTLLLAAALWPSIPWFGKTENHIAAIQARGVLRVSTIDSPLTYSVINGKKYGLDYELAQQFANYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYDSARVKNYQPGPMYYSVSQQLVYRVGQYRPRSLATVNENQLTIAPGHVV
VNDLQRLKETKFPDLSWKVDDKKGSTTLLEEVISGKLDYTIADSVAISLFQRVHPELAVALDVTDEQPVTWFSRLDDDNT
LSAALLDFFNSINEDGSLARIEEKYLGHGDDFDYVDTRSFLRAVDNVLPELEPLFKKYAKEIDWRLLAAISYQESHWDPL
ATSPTGVRGLMMLTKNTAQSLGLTDRTDAEQSISGGARYLEDMMAKVPETVPEDERIWFALAAYNMGYAHMLDARSLTVK
TKGNPDSWTDVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQEAEAMKLAQDYPAV
>Mature_475_residues
MKKLKINYLFIGILTLLLAAALWPSIPWFGKTENHIAAIQARGVLRVSTIDSPLTYSVINGKKYGLDYELAQQFANYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYDSARVKNYQPGPMYYSVSQQLVYRVGQYRPRSLATVNENQLTIAPGHVV
VNDLQRLKETKFPDLSWKVDDKKGSTTLLEEVISGKLDYTIADSVAISLFQRVHPELAVALDVTDEQPVTWFSRLDDDNT
LSAALLDFFNSINEDGSLARIEEKYLGHGDDFDYVDTRSFLRAVDNVLPELEPLFKKYAKEIDWRLLAAISYQESHWDPL
ATSPTGVRGLMMLTKNTAQSLGLTDRTDAEQSISGGARYLEDMMAKVPETVPEDERIWFALAAYNMGYAHMLDARSLTVK
TKGNPDSWTDVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQEAEAMKLAQDYPAV

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=476, Percent_Identity=86.9747899159664, Blast_Score=848, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 53648; Mature: 53648

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKINYLFIGILTLLLAAALWPSIPWFGKTENHIAAIQARGVLRVSTIDSPLTYSVIN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEEEEC
GKKYGLDYELAQQFANYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYDSARVKNYQ
CCCCCCCHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCHHEEEEHHHCCCHHCCCCC
PGPMYYSVSQQLVYRVGQYRPRSLATVNENQLTIAPGHVVVNDLQRLKETKFPDLSWKVD
CCCCEEHHHHHHHHHHHCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEC
DKKGSTTLLEEVISGKLDYTIADSVAISLFQRVHPELAVALDVTDEQPVTWFSRLDDDNT
CCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCH
LSAALLDFFNSINEDGSLARIEEKYLGHGDDFDYVDTRSFLRAVDNVLPELEPLFKKYAK
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAISYQESHWDPLATSPTGVRGLMMLTKNTAQSLGLTDRTDAEQSISGGARYL
HHCHHHHHEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCHHHHCCHHHHH
EDMMAKVPETVPEDERIWFALAAYNMGYAHMLDARSLTVKTKGNPDSWTDVKQRLPLLSQ
HHHHHHCCCCCCCCCCEEEEEEECCCCHHHEECCCEEEEEECCCCCHHHHHHHHCCCCCC
KPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQEAEAMKLAQDYPAV
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKLKINYLFIGILTLLLAAALWPSIPWFGKTENHIAAIQARGVLRVSTIDSPLTYSVIN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEEEEC
GKKYGLDYELAQQFANYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYDSARVKNYQ
CCCCCCCHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCHHEEEEHHHCCCHHCCCCC
PGPMYYSVSQQLVYRVGQYRPRSLATVNENQLTIAPGHVVVNDLQRLKETKFPDLSWKVD
CCCCEEHHHHHHHHHHHCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEC
DKKGSTTLLEEVISGKLDYTIADSVAISLFQRVHPELAVALDVTDEQPVTWFSRLDDDNT
CCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCH
LSAALLDFFNSINEDGSLARIEEKYLGHGDDFDYVDTRSFLRAVDNVLPELEPLFKKYAK
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAISYQESHWDPLATSPTGVRGLMMLTKNTAQSLGLTDRTDAEQSISGGARYL
HHCHHHHHEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCHHHHCCHHHHH
EDMMAKVPETVPEDERIWFALAAYNMGYAHMLDARSLTVKTKGNPDSWTDVKQRLPLLSQ
HHHHHHCCCCCCCCCCEEEEEEECCCCHHHEECCCEEEEEECCCCCHHHHHHHHCCCCCC
KPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQEAEAMKLAQDYPAV
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA