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The map label for this gene is lepA [H]

Identifier: 29140808

GI number: 29140808

Start: 312191

End: 313990

Strand: Direct

Name: lepA [H]

Synonym: t0274

Alternate gene names: 29140808

Gene position: 312191-313990 (Clockwise)

Preceding gene: 29140807

Following gene: 29140809

Centisome position: 6.51

GC content: 52.89

Gene sequence:

>1800_bases
ATGAAGAACATACGTAACTTTTCGATCATTGCTCACATTGACCACGGTAAATCGACGCTGTCTGACCGTATTATCCAAAT
CTGCGGTGGCCTGTCTGACCGTGAAATGGAAGCTCAGGTACTTGATTCGATGGATCTTGAGCGTGAGCGCGGTATTACTA
TTAAAGCCCAGAGTGTGACGCTGGATTTTAAAGCGTCTGATGGTGAAACTTATCAACTGAACTTTATCGACACGCCGGGA
CACGTTGACTTTTCCTATGAAGTTTCCCGTTCGTTAGCCGCCTGCGAGGGCGCGCTGCTGGTGGTGGATGCCGGCCAGGG
CGTAGAAGCGCAAACGTTGGCGAACTGCTACACCGCGATGGAAATGGATCTTGAAGTGGTGCCGGTGCTTAACAAGATTG
ACCTGCCGGCCGCCGATCCGGAGCGTGTGGCGGAAGAAATCGAAGACATTGTCGGCATCGATGCGACGGACGCGGTACGC
TGCTCCGCCAAAACGGGTGTCGGCGTGACGGACGTTCTGGAACGCCTGGTGCGCGATATCCCGCCGCCGCAAGGCGATCC
GGACGGCCCGCTGCAGGCGCTGATTATTGACTCCTGGTTCGATAACTACCTGGGCGTGGTATCCCTGGTGCGTATTAAAA
ACGGCACCATGCGTAAAGGCGACAAAATTAAAGTGATGAGCACCGGGCAGACCTACAACGCTGACCGCCTGGGGATCTTC
ACGCCGAAACAGGTTGATCGTACCGAGCTGAAGTGCGGCGAAGTAGGCTGGCTGGTCTGCGCCATTAAAGATATCCTCGG
CGCGCCGGTTGGCGATACCTTAACCTCAGCGCGTAACCCAGCTGAAAAAGCGTTGCCGGGCTTTAAGAAGGTGAAACCGC
AGGTCTATGCAGGTCTGTTCCCGGTCAGCTCCGACGATTATGAAAGTTTCCGCGACGCGCTCGGCAAGCTGAGCCTGAAC
GATGCCTCACTGTTTTATGAACCGGAAAGCTCCTCGGCGCTGGGCTTTGGTTTCCGCTGCGGCTTCCTCGGCCTGTTGCA
CATGGAGATCATTCAGGAGCGTCTGGAACGCGAATACGATCTGGATCTGATCACCACTGCGCCGACCGTGGTTTATGAAG
TAGAAACAACGGCGAAAGAGACTATCTATGTTGATAGCCCCTCCAAGCTGCCGCCGTTGAATAACATTTATGAACTGCGC
GAGCCTATCGCCGAATGTCATATGCTGTTACCACAAGCCTATTTAGGTAACGTTATTACGCTGTGTATTGAGAAACGCGG
CGTACAAACTAACATGGTGTATCACGGTAACCAGGTTGCGTTGACCTATGAAATCCCGATGGCGGAAGTGGTGCTCGACT
TCTTTGACCGTCTGAAATCAACGTCGCGCGGCTATGCGTCTCTGGATTATAACTTCAAGCGCTTCCAGGCTTCCGATATG
GTGCGTGTTGATGTGTTAATCAACAACGAGCGTGTCGATGCGCTGGCGCTGATCACGCACCGTGATAACTCGCAAAGCCG
TGGTCGCGAGCTGGTGGAGAAAATGAAAGATTTGATTCCACGCCAGCAGTTTGATATCGCGATTCAGGCGGCGATTGGTA
CGCATATTATTGCCCGTTCGACGGTAAAACAGTTACGTAAAAACGTGCTGGCGAAGTGCTACGGCGGCGATATCAGTCGT
AAGAAAAAACTGCTGCAGAAACAGAAAGAAGGTAAGAAACGCATGAAGCAGATCGGTAACGTCGAGCTGCCTCAGGAGGC
GTTCCTCGCCATTCTGCATGTCGGTAAAGACAATAAATAA

Upstream 100 bases:

>100_bases
TAGTGTAGAATGCGGCGTTTCAGTTAAAACAGACGTTAAGCTCAGAACAGCGACCTCTAAAGTCTGGTCAACCAGGCGTA
AGGCATAATAATTTATCTCT

Downstream 100 bases:

>100_bases
TCCCTAAGGAGTTGGCATGGCGAACATGTTTGCCCTGATTCTGGTGATAGCCACACTGGTGACGGGCATTTTATGGTGCG
TTGATAAGTTTGTTTTCGCG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDFKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
CSAKTGVGVTDVLERLVRDIPPPQGDPDGPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTSARNPAEKALPGFKKVKPQVYAGLFPVSSDDYESFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTAKETIYVDSPSKLPPLNNIYELR
EPIAECHMLLPQAYLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINNERVDALALITHRDNSQSRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK

Sequences:

>Translated_599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDFKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
CSAKTGVGVTDVLERLVRDIPPPQGDPDGPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTSARNPAEKALPGFKKVKPQVYAGLFPVSSDDYESFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTAKETIYVDSPSKLPPLNNIYELR
EPIAECHMLLPQAYLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINNERVDALALITHRDNSQSRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
>Mature_599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDFKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
CSAKTGVGVTDVLERLVRDIPPPQGDPDGPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTSARNPAEKALPGFKKVKPQVYAGLFPVSSDDYESFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTAKETIYVDSPSKLPPLNNIYELR
EPIAECHMLLPQAYLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINNERVDALALITHRDNSQSRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=47.5873544093178, Blast_Score=599, Evalue=1e-171,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.1127819548872, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=44.2953020134228, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=43.421052631579, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=43.421052631579, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI18390331, Length=152, Percent_Identity=36.1842105263158, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI53729339, Length=237, Percent_Identity=29.535864978903, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI53729337, Length=237, Percent_Identity=29.535864978903, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=32.3353293413174, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=32.3353293413174, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=96.9949916527546, Blast_Score=1160, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=506, Percent_Identity=29.0513833992095, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=35.4838709677419, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=29.2576419213974, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790412, Length=327, Percent_Identity=24.7706422018349, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789737, Length=327, Percent_Identity=24.7706422018349, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=41.2724306688418, Blast_Score=487, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=457, Percent_Identity=24.72647702407, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=37.6712328767123, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=228, Percent_Identity=31.140350877193, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=301, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=27.9166666666667, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566303, Length=246, Percent_Identity=27.6422764227642, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=45.5906821963394, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=43.75, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=43.75, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.8131868131868, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=40.2777777777778, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=283, Percent_Identity=24.7349823321555, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=283, Percent_Identity=24.7349823321555, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=25.2032520325203, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=42.2628951747088, Blast_Score=527, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.7536231884058, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=35.3383458646617, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28572034, Length=245, Percent_Identity=29.7959183673469, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363316, Length=281, Percent_Identity=25.6227758007117, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=281, Percent_Identity=25.6227758007117, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66539; Mature: 66539

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDFKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVTDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPQGDPDGPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQTYNADRLGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTSARNPAEKALPGFKKVKPQVYAGLF
CCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHCCCHHHCCCHHHEEEC
PVSSDDYESFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTAKETIYVDSPSKLPPLNNIYELREPIAECHMLLPQAYLGNVIT
CEEEECCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINNERVDALALITHRDNSQSRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARS
EEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHCEEEEHHHHHHHHHHH
TVKQLRKNVLAKCYGGDISRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDFKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVTDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPQGDPDGPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQTYNADRLGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTSARNPAEKALPGFKKVKPQVYAGLF
CCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHCCCHHHCCCHHHEEEC
PVSSDDYESFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTAKETIYVDSPSKLPPLNNIYELREPIAECHMLLPQAYLGNVIT
CEEEECCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINNERVDALALITHRDNSQSRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARS
EEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHCEEEEHHHHHHHHHHH
TVKQLRKNVLAKCYGGDISRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA