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The map label for this gene is btuF [H]

Identifier: 29140747

GI number: 29140747

Start: 238268

End: 239068

Strand: Reverse

Name: btuF [H]

Synonym: t0207

Alternate gene names: 29140747

Gene position: 239068-238268 (Counterclockwise)

Preceding gene: 29140748

Following gene: 29140746

Centisome position: 4.99

GC content: 55.56

Gene sequence:

>801_bases
ATGGCTAAGCAAATGTTCAGGGCGCTGGGCGCCCTGCTGCTTACGCTCCCGGTGTGGCTTTACGCTGCGCCGCGCGTAAT
TACGCTCTCTCCCGCCAATACGGAGCTTGCTTTTGCCGCCGAGATTACGCCCGTTGGCGTCAGCAGCTATTCCGACTACC
CGCCCGAAGCGCAAAAAATAGAACAGGTCTCTACCTGGCAGGGAATGAATCTGGAGCGCATTGTGGCGCTGAAGCCGGAT
CTGGTCGTCGCCTGGCGCGGCGGTAATGCGGAACGCCAGGTAAACCAACTGACGTCATTAGGTATTAAGGTCATGTGGGT
GGACGCGGTAACCATCGAACAGATCGCCGACGCGCTCCGCCAACTGGCCGCCTGGAGCCCGCAGCCGGAAAAGGCTCAGC
AGGCAGCGCAGACATTGCTAAAAGAGTACGCCGCGCTCAAAGTCGAGTATGCCGGTAAAGCTAAAAAGCGCGTCTTTCTT
CAGTTTGGCATGAATCCTCTGTTTACCAGTGGTAAAGGGTCTATTCAACATCAGGTTTTGACGACCTGCGGCGGAGAAAA
CGTCTTTGCTGACAGCCGCGTGCCGTGGCCGCAAGTCAGCCGTGAGCAGGTGCTGGCAAGGCATCCCCAGGCCATTATCG
TGGCCGGAAAAGCGGGCGAAATTCTCAAAATTGAACAATACTGGGGAAACCTGTTAAAAATTCCGGTTATTCCGCTTAAC
AGCGACTGGTTTGAACGCGCAAGCCCGCGTATTATCCTCGCCGCAAAACAACTCTGTAATGCGCTTTCACAGGTGAATTA
G

Upstream 100 bases:

>100_bases
GTGGCCGATCAGCAATCGCACCTCAGCTTTGACGAATTCCTCGCGGTGGCGGCGAAACAGTCGACTCTGATGGTCGAAAC
GCTGGTACAGAAACTGGCGC

Downstream 100 bases:

>100_bases
AGCCTGTCTTACCAGGCTCCTAGTGGGGATTCGACAATGCTCGTCTATTGGCTGGATATTGTAGGCACGGCTGTATTTGC
TATCTCTGGCGTGTTGCTGG

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MAKQMFRALGALLLTLPVWLYAAPRVITLSPANTELAFAAEITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPD
LVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADALRQLAAWSPQPEKAQQAAQTLLKEYAALKVEYAGKAKKRVFL
QFGMNPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
SDWFERASPRIILAAKQLCNALSQVN

Sequences:

>Translated_266_residues
MAKQMFRALGALLLTLPVWLYAAPRVITLSPANTELAFAAEITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPD
LVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADALRQLAAWSPQPEKAQQAAQTLLKEYAALKVEYAGKAKKRVFL
QFGMNPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
SDWFERASPRIILAAKQLCNALSQVN
>Mature_265_residues
AKQMFRALGALLLTLPVWLYAAPRVITLSPANTELAFAAEITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPDL
VVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADALRQLAAWSPQPEKAQQAAQTLLKEYAALKVEYAGKAKKRVFLQ
FGMNPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNS
DWFERASPRIILAAKQLCNALSQVN

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=266, Percent_Identity=80.8270676691729, Blast_Score=440, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29340; Mature: 29209

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQMFRALGALLLTLPVWLYAAPRVITLSPANTELAFAAEITPVGVSSYSDYPPEAQKI
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
EQVSTWQGMNLERIVALKPDLVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADALR
HHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHH
QLAAWSPQPEKAQQAAQTLLKEYAALKVEYAGKAKKRVFLQFGMNPLFTSGKGSIQHQVL
HHHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCHHEEEEEECCCEEEECCCCCHHHHHH
TTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
HHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEEEEHHHHCCEEEEEEEECC
SDWFERASPRIILAAKQLCNALSQVN
CHHHHHCCCEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKQMFRALGALLLTLPVWLYAAPRVITLSPANTELAFAAEITPVGVSSYSDYPPEAQKI
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
EQVSTWQGMNLERIVALKPDLVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADALR
HHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHH
QLAAWSPQPEKAQQAAQTLLKEYAALKVEYAGKAKKRVFLQFGMNPLFTSGKGSIQHQVL
HHHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCHHEEEEEECCCEEEECCCCCHHHHHH
TTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
HHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEEEEHHHHCCEEEEEEEECC
SDWFERASPRIILAAKQLCNALSQVN
CHHHHHCCCEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA