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The map label for this gene is yadB [H]

Identifier: 29140734

GI number: 29140734

Start: 219832

End: 220728

Strand: Reverse

Name: yadB [H]

Synonym: t0193

Alternate gene names: 29140734

Gene position: 220728-219832 (Counterclockwise)

Preceding gene: 29140735

Following gene: 29140733

Centisome position: 4.61

GC content: 57.86

Gene sequence:

>897_bases
ATGACTGACTCACACTATATTGGCCGTTTCGCGCCGTCGCCATCCGGCGAACTGCATTTCGGCTCGTTAATCGCCGCCCT
CGGCAGCTATCTACAAGCCCGTGCGCAACGCGGAGTTTGGCGCGTTCGCATAGAGGATATCGACCCCCCACGTGAAGTTC
CCGGTGCCGCAGCGACCATTCTGCGCCAGCTGGAACATTACGGCCTGCACTGGGATGGCGAGGTCTTATGGCAATCACAG
CGTCATGAGGCTTATCGCGAGGCGCTGGCCTGGCTTCACGAGCAGGGACTGAGCTACTACTGCACTTGCCCCCGCTCGCG
CATTCAACGTCTGGGCGGTATTTACGATGGCCATTGCCGGACGTTATGCCACGGGCCGGAGAATGCCGCCGTCAGAATTA
AACAGCAGCATCCAGTGATGCATTTCCATGATGCCTTGCGCGGCGACATCCAGGCTGACCCACAGCTGGCTAGCGAAGAT
TTTATTATCCACCGCCGCGACGGACTGTTTGCTTATAACCTGGCCGTGGTGGTCGACGATCATTTTCAGGGGGTGACGGA
GATCGTCCGCGGCGCAGATTTAATCGAACCTACTGTCCGACAACTTTCGCTGTATAAACAGTTTGGCTGGCGTGCGCCAG
GTTACGTGCACCTTCCGCTGGCGCTCAACGAACAAGGCGCTAAACTTTCCAAGCAAAATCATGCCCCTGCGCTTGCGACA
GGCGATCCGCGCCCGGTACTGGTCCAGGCATTGCGCTTTTTAGGCCAACGCGACGTCGTAGCGTGGCAGGAGATGTCTGT
TGAGGAGTTGCTGCGCTTTGCGGTGGCGCACTGGAGGCTTACAGCCGTACCCACATCGGCTAATGTAAATCCGGCATTCT
CAAATGCGTCTCGCTGA

Upstream 100 bases:

>100_bases
CTGAAATTCGCGAAAAACAGATGGCGGGTTAATCCCGTTCGTTTCTACCCTATTTATGACAGGCGGGAGTTTTCCCGCCT
TATTCTTTATTGCCCGCAGC

Downstream 100 bases:

>100_bases
GCTATGATTAGCCGCTATTTTTGCTCATCGTCTTTCGCATTGCTGCGGCGTTAATGATGCTTACAGCCCAGGTCGCGTTG
CTCTCTTACGCCCGTCTGCC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTDSHYIGRFAPSPSGELHFGSLIAALGSYLQARAQRGVWRVRIEDIDPPREVPGAAATILRQLEHYGLHWDGEVLWQSQ
RHEAYREALAWLHEQGLSYYCTCPRSRIQRLGGIYDGHCRTLCHGPENAAVRIKQQHPVMHFHDALRGDIQADPQLASED
FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQLSLYKQFGWRAPGYVHLPLALNEQGAKLSKQNHAPALAT
GDPRPVLVQALRFLGQRDVVAWQEMSVEELLRFAVAHWRLTAVPTSANVNPAFSNASR

Sequences:

>Translated_298_residues
MTDSHYIGRFAPSPSGELHFGSLIAALGSYLQARAQRGVWRVRIEDIDPPREVPGAAATILRQLEHYGLHWDGEVLWQSQ
RHEAYREALAWLHEQGLSYYCTCPRSRIQRLGGIYDGHCRTLCHGPENAAVRIKQQHPVMHFHDALRGDIQADPQLASED
FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQLSLYKQFGWRAPGYVHLPLALNEQGAKLSKQNHAPALAT
GDPRPVLVQALRFLGQRDVVAWQEMSVEELLRFAVAHWRLTAVPTSANVNPAFSNASR
>Mature_297_residues
TDSHYIGRFAPSPSGELHFGSLIAALGSYLQARAQRGVWRVRIEDIDPPREVPGAAATILRQLEHYGLHWDGEVLWQSQR
HEAYREALAWLHEQGLSYYCTCPRSRIQRLGGIYDGHCRTLCHGPENAAVRIKQQHPVMHFHDALRGDIQADPQLASEDF
IIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQLSLYKQFGWRAPGYVHLPLALNEQGAKLSKQNHAPALATG
DPRPVLVQALRFLGQRDVVAWQEMSVEELLRFAVAHWRLTAVPTSANVNPAFSNASR

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=35.3413654618474, Blast_Score=138, Evalue=6e-33,
Organism=Escherichia coli, GI226510925, Length=297, Percent_Identity=81.1447811447811, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1788743, Length=301, Percent_Identity=31.5614617940199, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=33.8645418326693, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324540, Length=269, Percent_Identity=33.0855018587361, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24665321, Length=249, Percent_Identity=34.5381526104418, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 33553; Mature: 33422

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSHYIGRFAPSPSGELHFGSLIAALGSYLQARAQRGVWRVRIEDIDPPREVPGAAATI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHH
LRQLEHYGLHWDGEVLWQSQRHEAYREALAWLHEQGLSYYCTCPRSRIQRLGGIYDGHCR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCHH
TLCHGPENAAVRIKQQHPVMHFHDALRGDIQADPQLASEDFIIHRRDGLFAYNLAVVVDD
HEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEEEEEECC
HFQGVTEIVRGADLIEPTVRQLSLYKQFGWRAPGYVHLPLALNEQGAKLSKQNHAPALAT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHCCCCCEEEC
GDPRPVLVQALRFLGQRDVVAWQEMSVEELLRFAVAHWRLTAVPTSANVNPAFSNASR
CCCCHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDSHYIGRFAPSPSGELHFGSLIAALGSYLQARAQRGVWRVRIEDIDPPREVPGAAATI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHH
LRQLEHYGLHWDGEVLWQSQRHEAYREALAWLHEQGLSYYCTCPRSRIQRLGGIYDGHCR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCHH
TLCHGPENAAVRIKQQHPVMHFHDALRGDIQADPQLASEDFIIHRRDGLFAYNLAVVVDD
HEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEEEEEECC
HFQGVTEIVRGADLIEPTVRQLSLYKQFGWRAPGYVHLPLALNEQGAKLSKQNHAPALAT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHCCCCCEEEC
GDPRPVLVQALRFLGQRDVVAWQEMSVEELLRFAVAHWRLTAVPTSANVNPAFSNASR
CCCCHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA