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The map label for this gene is pcnB [H]

Identifier: 29140733

GI number: 29140733

Start: 218273

End: 219691

Strand: Reverse

Name: pcnB [H]

Synonym: t0192

Alternate gene names: 29140733

Gene position: 219691-218273 (Counterclockwise)

Preceding gene: 29140734

Following gene: 29140732

Centisome position: 4.58

GC content: 56.24

Gene sequence:

>1419_bases
ATGACACTACCGAGGTGCGCTATTTTTACCCGAGTCGCTAATTTTTGCCGTAAGGTGTTAAGCCGCGAGGAGAGCGAGGC
AGAACAAGCCGTCGCCCGTCCACATATGACGATTATCCCGCGTGAACAGCACGCTATCTCCCGCAAAGATATCAGTGAAA
ATGCCCTCAAGGTACTGTACAGGCTGAACAAAGCGGGCTATGAAGCCTACCTGGTCGGCGGCGGCGTCCGCGATCTCCTG
CTCGGTAAAAAGCCGAAGGATTTCGATGTGACCACCAACGCAACACCGGATCAGGTACGGAAATTATTCCGCAATTGCCG
TCTGGTGGGGCGTCGTTTCCGCCTGGCTCACGTGATGTTTGGCCCGGAAATTATCGAAGTGGCAACGTTTCGTGGTCATA
ATGAAGGCAGTGAAAGCGACCGTACGACCTCCCAGCGTGGGCAAAACGGTATGCTGCTGCGCGACAACATCTTCGGCTCT
ATCGAAGAAGATGCCCAGCGCCGCGATTTCACCATCAACAGCCTTTACTACAGCGTGGCGGATTTTACCGTGCGCGATTA
CGTCGGCGGGATGCAGGATCTGCAAGAAGGCGTGATTCGCCTGATCGGCAATCCGGAAACGCGCTACCGCGAAGATCCGG
TTCGAATGCTGCGCGCCGTGCGTTTCGCTGCGAAGCTCAATATGCGTATCAGCCCTGAAACGGCTGAGCCAATCCCGCGT
CTGGCAACCTTGCTAAACGACATTCCTCCCGCGCGCCTGTTCGAAGAATCGCTGAAGCTGTTGCAGGCGGGGAACGGTTT
TGAAACCTATCAACAACTGCGGGAATACCACCTCTTCCAGCCGTTGTTTCCTACCATTACGCGTTATTTCACCGAAAACG
GCGACAGCGCAATGGAACGCATCATTGCGCAGGTGTTGAAGAATACGGATAACCGCATCCGTAACGAGATGCGCGTTAAC
CCGGCATTTTTGTTTGCCGCCATGTTCTGGTATCCGCTGCTGGAGATGGCGCAAAAAATCGCTCAGGAGAGCGGCCTTGC
CTATTACGATGCTTTCGCGCTGGCCATGAATGACGTGCTGGATGAAGCCTGCCGTTCACTGGCGATCCCGAAACGCCTTA
CCACGCTTACCCGTGATATTTGGCAGCTTCAGTTACGCATGTCGCGACGCCAGGGCAAACGCGCCTGGAAGCTGATGGAA
CATCCCAAATTCCGCGCCGCGTTTGATTTGCTGGAGCTGCGCGCCCAAGTGGAAAATAATACTGAACTGCAACGTCTGGC
GCAGTGGTGGGCCGAGTTTCAGGCTTCCGCGCCGCCGGAACAAAAAGGGATGCTCAACGAGCTGGACGACGATCCTGCTC
CACGCCGCCGTCGTTCACGTCCGCGCAAACGCGCGCCGCGCCGCGAGGGCACCGTATGA

Upstream 100 bases:

>100_bases
TGCTGCGGCGTTAATGATGCTTACAGCCCAGGTCGCGTTGCTCTCTTACGCCCGTCTGCCGTAATGCGAAAGACATAGAG
TAAGTTGATCTTATTAATGT

Downstream 100 bases:

>100_bases
CGATCGCGTATATCGCGCTTGGCAGTAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCCGCCCTGAAGGCTATCGCCGAC
ATTCCCGATAGCCGTATCGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLL
LGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPR
LATLLNDIPPARLFEESLKLLQAGNGFETYQQLREYHLFQPLFPTITRYFTENGDSAMERIIAQVLKNTDNRIRNEMRVN
PAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLME
HPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV

Sequences:

>Translated_472_residues
MTLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLL
LGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPR
LATLLNDIPPARLFEESLKLLQAGNGFETYQQLREYHLFQPLFPTITRYFTENGDSAMERIIAQVLKNTDNRIRNEMRVN
PAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLME
HPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
>Mature_471_residues
TLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLL
GKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGSI
EEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPRL
ATLLNDIPPARLFEESLKLLQAGNGFETYQQLREYHLFQPLFPTITRYFTENGDSAMERIIAQVLKNTDNRIRNEMRVNP
AFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEH
PKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=275, Percent_Identity=30.1818181818182, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI87081691, Length=444, Percent_Identity=90.990990990991, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=227, Percent_Identity=33.0396475770925, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=287, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=241, Percent_Identity=32.7800829875519, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357337, Length=241, Percent_Identity=32.7800829875519, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54657; Mature: 54526

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLY
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHH
LATLLNDIPPARLFEESLKLLQAGNGFETYQQLREYHLFQPLFPTITRYFTENGDSAMER
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IIAQVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVL
HHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
TELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
HHHHHHHHHHHHHCCCCCCHHCCCHHHCCCCCCCHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
TLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLY
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHH
LATLLNDIPPARLFEESLKLLQAGNGFETYQQLREYHLFQPLFPTITRYFTENGDSAMER
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IIAQVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVL
HHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
TELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
HHHHHHHHHHHHHCCCCCCHHCCCHHHCCCCCCCHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]