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The map label for this gene is speE [H]

Identifier: 29140712

GI number: 29140712

Start: 197195

End: 198055

Strand: Reverse

Name: speE [H]

Synonym: t0171

Alternate gene names: 29140712

Gene position: 198055-197195 (Counterclockwise)

Preceding gene: 29140713

Following gene: 29140711

Centisome position: 4.13

GC content: 53.66

Gene sequence:

>861_bases
ATGGCCGAAAATACGATGTGGCATGAAACGCTACACGACCAGTTTGGTCAGTACTTTGCCGTAGACAACGTTCTGTATCA
TGAAAAAACCGATCACCAGGATTTAATCATCTTTGAAAACGCCGCTTTTGGCCGCGTGATGGCGCTGGACGGCGTGGTAC
AGACCACCGAACGCGACGAGTTTATTTATCATGAAATGATGACCCATGTCCCGCTGCTGGCGCACGGTCATGCGAAACAC
GTTCTGATTATCGGCGGCGGCGACGGCGCAATGCTACGTGAAGTTACCCGGCATAAAAACGTCGAAACCATCACCATGGT
AGAGATAGACGCTGGCGTAGTCTCGTTTTGCCGCCAGTATTTACCCAACCACAATGCCGGCAGCTACGACGATCCGCGCT
TTACGCTGGTGATCGACGACGGCGTGAATTTCGTCAATCAGACCCATCAGACCTTTGATGTGATCATTTCCGACTGTACC
GATCCGATAGGCCCCGGCGAAAGCCTGTTTACCTCCGCCTTTTATGAAGGCTGTAAGCGTTGCCTGAATCCGGGCGGCAT
TTTTGTCGCCCAGAACGGCGTGTGCTTCCTGCAACAGGATGAAGCGCTCGACAGCCACCGCAAGCTGAGTCATTACTTCA
GCGATGTCGGCTTCTACCAGGCGGCGATTCCGACCTATTACGGCGGCATTATGACCTTCGCCTGGGCGACGGACAACGAC
GCTCTTCGCCACCTGTCGAGCGAAATTATTCAGGCGCGTTTTCATGCCGCCGGCCTCAAATGCCGCTATTACAACTCGGC
TATTCATGCCGCTGCATTTGCGCTACCGCAATATCTACATGACGCACTGTCCGCACAGTGA

Upstream 100 bases:

>100_bases
GCGATCCTATTGCTGAAATAATGACCGTGCATCCACGGTCATTGTTAGCTATGATGTTGCGCCCTTTTTTTACGGGTGTT
AACAATGGAGGTATCAGCCA

Downstream 100 bases:

>100_bases
CGTTAAGGAGGTGATAAAAATTGAAAAAGCTGAAACTGCATGGCTTTAACAATCTGACGAAAAGCCTGAGTTTTTGTATT
TACGATATCTGCTACGCCAA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAENTMWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKH
VLIIGGGDGAMLREVTRHKNVETITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFTLVIDDGVNFVNQTHQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQDEALDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
ALRHLSSEIIQARFHAAGLKCRYYNSAIHAAAFALPQYLHDALSAQ

Sequences:

>Translated_286_residues
MAENTMWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKH
VLIIGGGDGAMLREVTRHKNVETITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFTLVIDDGVNFVNQTHQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQDEALDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
ALRHLSSEIIQARFHAAGLKCRYYNSAIHAAAFALPQYLHDALSAQ
>Mature_285_residues
AENTMWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHV
LIIGGGDGAMLREVTRHKNVETITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFTLVIDDGVNFVNQTHQTFDVIISDCTD
PIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQDEALDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDNDA
LRHLSSEIIQARFHAAGLKCRYYNSAIHAAAFALPQYLHDALSAQ

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=266, Percent_Identity=36.8421052631579, Blast_Score=204, Evalue=5e-53,
Organism=Escherichia coli, GI1786312, Length=286, Percent_Identity=94.4055944055944, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI25154106, Length=281, Percent_Identity=41.2811387900356, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI212645616, Length=325, Percent_Identity=34.4615384615385, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=283, Percent_Identity=38.86925795053, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325326, Length=289, Percent_Identity=36.3321799307958, Blast_Score=189, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24645443, Length=281, Percent_Identity=37.3665480427046, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=36.2790697674419, Blast_Score=147, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32085; Mature: 31954

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENTMWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDE
CCCCHHHHHHHHHHHCCEEECCCEEECCCCCCCCEEEEECCCCCEEEECCCHHHCCCCHH
FIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVETITMVEIDAGVVSFCRQY
HHHHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGSYDDPRFTLVIDDGVNFVNQTHQTFDVIISDCTDPIGPGESLFTSAFYEGCKR
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CLNPGGIFVAQNGVCFLQQDEALDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
HCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ALRHLSSEIIQARFHAAGLKCRYYNSAIHAAAFALPQYLHDALSAQ
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AENTMWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDE
CCCHHHHHHHHHHHCCEEECCCEEECCCCCCCCEEEEECCCCCEEEECCCHHHCCCCHH
FIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVETITMVEIDAGVVSFCRQY
HHHHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGSYDDPRFTLVIDDGVNFVNQTHQTFDVIISDCTDPIGPGESLFTSAFYEGCKR
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CLNPGGIFVAQNGVCFLQQDEALDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
HCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ALRHLSSEIIQARFHAAGLKCRYYNSAIHAAAFALPQYLHDALSAQ
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA