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The map label for this gene is murF [H]

Identifier: 29140669

GI number: 29140669

Start: 146369

End: 147727

Strand: Direct

Name: murF [H]

Synonym: t0128

Alternate gene names: 29140669

Gene position: 146369-147727 (Clockwise)

Preceding gene: 29140668

Following gene: 29140670

Centisome position: 3.05

GC content: 56.51

Gene sequence:

>1359_bases
ATGATTAGCGTAACGCTCAGCAAAATTGCCGATGTCCTTGGCGCCGAACACCGCGGGGCGGATCTCACGCTCGATACGGT
CATTACCGACACACGGAAAGTGACGCCGGGATGCCTGTTCGTGGCGCTGAAAGGGGAACGCTTTGATGCGCATGATTTTG
CTGATAAAGCAAAAGCGAACGGCGCTGGCGCCCTGCTGGTCAGTCGTCCTCTGGATATCGATCTTCCGCAGGTGATTGTA
AAAGACACGCGTCAGGCATTTGGCCAACTGGCCGCCTGGGTACGTATGCAGGTACCGGCGCGGGTGGTGGCGTTAACCGG
TTCGTCCGGCAAAACCTCGGTCAAAGAGATGACCGCCGCGATTCTTAGCCAGTGCGGAAATACTTTGTATACCGCAAGCA
ATTTTAACAACGACATCGGCGTGCCGATAACGCTGCTGCGCTTAAACCATGATTATGACTATGCGGTGATCGAGTTAGGC
GCTAATCACCAGGGAGAAATCGCCTGGACCGTTAGCCTGACGCGCCCGGAAGCGGCGCTGGTCAATAACCTGGCGGCGGC
GCATCTGGAAGGTTTCGGCTCGCTGGCCGGCGTGGCGAAAGCGAAAGGCGAAATCTATACCGGTTTGCCTGAGAACGGTA
TCGCGATTATGAATGCCGATAATAATGACTGGCTGAACTGGCAAAGCATTATTGGCGATCGTCAGGTGTGGCGCTTTTCG
CCGAATGCGGCAAACAGCGACTTTACCGCTGCGAATATCCACGTGACGTCACACGGTACCGAATTTACCCTGCAAACGCC
GATGGGCAGCATTGATGTGCTGCTGCCGTTGCCGGGGCGGCACAATATTGCCAACGCGCTGGCGGCCGCTGCTTTATCAA
TGGCGGTGGGGGCGACGCTGGCGGCGGTTAAAGCAGGGCTGGCGGCGTTAAAAGCCGTACCCGGGCGTCTGTTCCCGATT
CAACTAAGTGAAAACCAACTGGTACTGGATGACGCTTATAACGCCAACGTCGGTTCGATGACCGCCGCAGTGCAGGTCCT
TTCTGAAATGCCCGGCTACCGCGTGCTGGTGGTAGGCGATATGGCCGAGCTGGGCGCAGAAAGCGAAGCGTGCCACCTCC
AGGTTGGCGAGGCCGCAAAAGCGGCGGGTATTGATCGCGTGTTAAGCACCGGAAAACTCAGTCAGGTTATCAGCCACGCC
AGCGGCGTCGGCGAACATTTTGCCGATAAAGCCGCGCTCATTGCGCGTTTACACGCTCTGCTTCAGGAGCAACCGATGAT
GACTATTTTAGTGAAAGGATCACGCAGCGCCGCGATGGAAGATGTGGTTCATGCGTTACAGGAGAAAGGTTCATGTTAG

Upstream 100 bases:

>100_bases
TGATTGCAGGTAAAGGGCACGAGGATTACCAGATTGTCGGCACGCAGCGTCTTGATTATTCAGACCGCGTGACCGCAGCG
CGTTTGCTGGGGGTGATCGC

Downstream 100 bases:

>100_bases
TTTGGCTGGCCGAGCATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCTTATCTGACGTTTCGCGCCATCGTCAGC
CTGTTGACCGCGCTGTTCAT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MISVTLSKIADVLGAEHRGADLTLDTVITDTRKVTPGCLFVALKGERFDAHDFADKAKANGAGALLVSRPLDIDLPQVIV
KDTRQAFGQLAAWVRMQVPARVVALTGSSGKTSVKEMTAAILSQCGNTLYTASNFNNDIGVPITLLRLNHDYDYAVIELG
ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIYTGLPENGIAIMNADNNDWLNWQSIIGDRQVWRFS
PNAANSDFTAANIHVTSHGTEFTLQTPMGSIDVLLPLPGRHNIANALAAAALSMAVGATLAAVKAGLAALKAVPGRLFPI
QLSENQLVLDDAYNANVGSMTAAVQVLSEMPGYRVLVVGDMAELGAESEACHLQVGEAAKAAGIDRVLSTGKLSQVISHA
SGVGEHFADKAALIARLHALLQEQPMMTILVKGSRSAAMEDVVHALQEKGSC

Sequences:

>Translated_452_residues
MISVTLSKIADVLGAEHRGADLTLDTVITDTRKVTPGCLFVALKGERFDAHDFADKAKANGAGALLVSRPLDIDLPQVIV
KDTRQAFGQLAAWVRMQVPARVVALTGSSGKTSVKEMTAAILSQCGNTLYTASNFNNDIGVPITLLRLNHDYDYAVIELG
ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIYTGLPENGIAIMNADNNDWLNWQSIIGDRQVWRFS
PNAANSDFTAANIHVTSHGTEFTLQTPMGSIDVLLPLPGRHNIANALAAAALSMAVGATLAAVKAGLAALKAVPGRLFPI
QLSENQLVLDDAYNANVGSMTAAVQVLSEMPGYRVLVVGDMAELGAESEACHLQVGEAAKAAGIDRVLSTGKLSQVISHA
SGVGEHFADKAALIARLHALLQEQPMMTILVKGSRSAAMEDVVHALQEKGSC
>Mature_452_residues
MISVTLSKIADVLGAEHRGADLTLDTVITDTRKVTPGCLFVALKGERFDAHDFADKAKANGAGALLVSRPLDIDLPQVIV
KDTRQAFGQLAAWVRMQVPARVVALTGSSGKTSVKEMTAAILSQCGNTLYTASNFNNDIGVPITLLRLNHDYDYAVIELG
ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIYTGLPENGIAIMNADNNDWLNWQSIIGDRQVWRFS
PNAANSDFTAANIHVTSHGTEFTLQTPMGSIDVLLPLPGRHNIANALAAAALSMAVGATLAAVKAGLAALKAVPGRLFPI
QLSENQLVLDDAYNANVGSMTAAVQVLSEMPGYRVLVVGDMAELGAESEACHLQVGEAAKAAGIDRVLSTGKLSQVISHA
SGVGEHFADKAALIARLHALLQEQPMMTILVKGSRSAAMEDVVHALQEKGSC

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=86.9469026548673, Blast_Score=773, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47563; Mature: 47563

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVTLSKIADVLGAEHRGADLTLDTVITDTRKVTPGCLFVALKGERFDAHDFADKAKAN
CEEEEHHHHHHHHCCCCCCCCEEEHHEECCCCCCCCCEEEEEEECCCCCCHHHHHHHCCC
GAGALLVSRPLDIDLPQVIVKDTRQAFGQLAAWVRMQVPARVVALTGSSGKTSVKEMTAA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCHHHHHHHHH
ILSQCGNTLYTASNFNNDIGVPITLLRLNHDYDYAVIELGANHQGEIAWTVSLTRPEAAL
HHHHCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCHHHH
VNNLAAAHLEGFGSLAGVAKAKGEIYTGLPENGIAIMNADNNDWLNWQSIIGDRQVWRFS
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCCHHHHHCCCEEEEEC
PNAANSDFTAANIHVTSHGTEFTLQTPMGSIDVLLPLPGRHNIANALAAAALSMAVGATL
CCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AAVKAGLAALKAVPGRLFPIQLSENQLVLDDAYNANVGSMTAAVQVLSEMPGYRVLVVGD
HHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
MAELGAESEACHLQVGEAAKAAGIDRVLSTGKLSQVISHASGVGEHFADKAALIARLHAL
HHHHCCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LQEQPMMTILVKGSRSAAMEDVVHALQEKGSC
HHCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISVTLSKIADVLGAEHRGADLTLDTVITDTRKVTPGCLFVALKGERFDAHDFADKAKAN
CEEEEHHHHHHHHCCCCCCCCEEEHHEECCCCCCCCCEEEEEEECCCCCCHHHHHHHCCC
GAGALLVSRPLDIDLPQVIVKDTRQAFGQLAAWVRMQVPARVVALTGSSGKTSVKEMTAA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCHHHHHHHHH
ILSQCGNTLYTASNFNNDIGVPITLLRLNHDYDYAVIELGANHQGEIAWTVSLTRPEAAL
HHHHCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCHHHH
VNNLAAAHLEGFGSLAGVAKAKGEIYTGLPENGIAIMNADNNDWLNWQSIIGDRQVWRFS
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCCHHHHHCCCEEEEEC
PNAANSDFTAANIHVTSHGTEFTLQTPMGSIDVLLPLPGRHNIANALAAAALSMAVGATL
CCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AAVKAGLAALKAVPGRLFPIQLSENQLVLDDAYNANVGSMTAAVQVLSEMPGYRVLVVGD
HHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
MAELGAESEACHLQVGEAAKAAGIDRVLSTGKLSQVISHASGVGEHFADKAALIARLHAL
HHHHCCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LQEQPMMTILVKGSRSAAMEDVVHALQEKGSC
HHCCCEEEEEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]