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The map label for this gene is leuA [H]

Identifier: 29140658

GI number: 29140658

Start: 133572

End: 135143

Strand: Reverse

Name: leuA [H]

Synonym: t0117

Alternate gene names: 29140658

Gene position: 135143-133572 (Counterclockwise)

Preceding gene: 29140659

Following gene: 29140657

Centisome position: 2.82

GC content: 55.28

Gene sequence:

>1572_bases
ATGAGCCAGCAAGTCATTATTTTTGATACGACCTTACGCGACGGCGAACAAGCGTTACAGGCCAGCCTGAGCGCGAAAGA
GAAGCTGCAGATTGCCCTGGCCCTTGAGCGCATGGGTGTCGATGTCATGGAAGTGGGCTTCCCGGTCTCTTCTCCGGGTG
ATTTTGAATCCGTCCAAACCATCGCCCGCACCATCAAAAACAGCCGCGTTTGCGCCCTGGCGCGTTGTGTAGAAAAAGAC
ATCGACGTTGCGGCACAGGCGCTGAAGGTCGCCGACGCGTTTCGTATCCATACTTTTATCGCCACCTCGCCAATGCACAT
CGCCACCAAGCTGCGCAGCACGCTGGATGAGGTCATCGAACGCGCGGTATACATGGTTAAGCGGGCGCGTAATTACACTG
ATGACGTGGAGTTCTCCTGCGAGGATGCTGGCCGGACGCCTGTTGACGATCTGGCGCGCGTGGTGGAAGCCGCTATAAAC
GCCGGCGCGAGAACCATCAATATTCCCGATACCGTGGGCTATACCATGCCGTTTGAGTTCGCGGGAATCATTTCCGGTCT
GTATGAACGTGTCCCCAATATCGACAAAGCTATCATCTCCGTTCATACCCACGACGATTTAGGGATAGCGGTAGGCAACT
CGCTGGCGGCAGTACATGCGGGCGCGCGTCAAGTTGAAGGCGCGATGAACGGTATCGGAGAACGTGCCGGTAACTGCGCG
CTGGAAGAGGTGATCATGGCGATTAAAGTGCGCAAAGACATCATAAACGTACACACCAACATCAATCACCACGAAATCTG
GCGCACCAGCCAGACCGTCAGCCAGATCTGTAACATGCCGATCCCGGCCAACAAAGCGATTGTTGGCAGCGGCGCTTTTG
CCCACTCTTCCGGCATCCACCAGGATGGCGTCCTCAAGAACCGCGAAAACTATGAAATCATGACGCCAGAGTCCATCGGT
CTGAACCAGATACAGCTGAACCTGACCTCCCGCTCTGGCCGTGCCGCCGTGAAACATCGCATGGAAGAGATGGGTTACAA
GGACACAGACTACAACATGGGCCACCTGTACGACGCGTTCCTGAAGCTGGCGGACAAAAAAGGCCAGGTGTTCGACTATG
ACCTGGAAGCGCTGGCGTTTATTAATAAACAGCAAGAAGAACCAGAGCATTTCCGTCTGGATTACTTCAGCGTGCAGTCC
GGCTCCAGCGATATCGCCACCGCTTCCGTGAAGCTGGCCTGTGGTGAAGAGATCAAGGCCGAGGCGGCGAACGGCAACGG
CCCGGTCGACGCCATCTATCAGGCGATTAACCGCATCACTGGCTACGACGTTGAGTTGGTTAAATACGACCTGAACGCCA
AAGGCCAGGGCAAAGACGCGCTGGGTCAGGTCGATATCGTCGTGAACCATCATGGTCGCCGCTTCCACGGCGTCGGGCTG
GCGACGGATATCGTCGAATCCTCCGCCAAAGCGATGGTACACGTACTGAACAATATCTGGCGCGCCGCCGAAGTTGAAAA
AGAATTGCAACGCAAAGCTCAAAATAAAGAGAACAACAAGGAAACCGTGTAA

Upstream 100 bases:

>100_bases
CATTCAACATTAAGTCAGCTCGAAGTCAAACAAAACCCGCGCCGTTGTGCGGGTTTTTTTATGCCTGACGCAAGGCGCCC
CTGGAGACAAGGACCACATC

Downstream 100 bases:

>100_bases
TGTCGAAGAACTATCATATTGCCGTACTGCCGGGCGACGGTATCGGCCCGGAAGTCATGGCGCAAGCCCTGAAAGTTATG
GATGCGGTACGCAGCCGTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARTIKNSRVCALARCVEKD
IDVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIERAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAIN
AGARTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCA
LEEVIMAIKVRKDIINVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIG
LNQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMGHLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQS
GSSDIATASVKLACGEEIKAEAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV

Sequences:

>Translated_523_residues
MSQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARTIKNSRVCALARCVEKD
IDVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIERAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAIN
AGARTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCA
LEEVIMAIKVRKDIINVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIG
LNQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMGHLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQS
GSSDIATASVKLACGEEIKAEAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
>Mature_522_residues
SQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARTIKNSRVCALARCVEKDI
DVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIERAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAINA
GARTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCAL
EEVIMAIKVRKDIINVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGL
NQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMGHLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQSG
SSDIATASVKLACGEEIKAEAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGLA
TDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=523, Percent_Identity=92.7342256214149, Blast_Score=1013, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=27.5766016713092, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=27.8551532033426, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=542, Percent_Identity=24.9077490774908, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=543, Percent_Identity=24.8618784530387, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57356; Mature: 57224

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IARTIKNSRVCALARCVEKDIDVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAINAGARTINIPDTVGYTMPFEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
AGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCA
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHH
LEEVIMAIKVRKDIINVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMGHLYDAF
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQSGSSDIATASVKLACGEEIKA
HHHHCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCHHHC
EAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEECCCH
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IARTIKNSRVCALARCVEKDIDVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAINAGARTINIPDTVGYTMPFEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
AGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCA
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHH
LEEVIMAIKVRKDIINVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMGHLYDAF
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQSGSSDIATASVKLACGEEIKA
HHHHCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCHHHC
EAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEECCCH
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA