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The map label for this gene is rnjA [H]

Identifier: 28378771

GI number: 28378771

Start: 1949950

End: 1951626

Strand: Direct

Name: rnjA [H]

Synonym: lp_2158

Alternate gene names: 28378771

Gene position: 1949950-1951626 (Clockwise)

Preceding gene: 28378770

Following gene: 28378772

Centisome position: 58.94

GC content: 45.44

Gene sequence:

>1677_bases
ATGAAACGGTTAAACGTCAAAAATAACGAAACCGCTGTTTTTGCAGTCGGCGGTTTGGGAGAAATTGGGAAAAATACCTA
TGGTATCCAATTTCAAGATGAGATTATTCTGATTGATGCCGGAATTAAATTCCCTGAAGATGAACTTCTCGGAATTGATT
ACGTTATTCCAGACTATTCTTATCTAGTTGCAAATCAGCAAAAAATAAAAGCTTTAGTGATTACGCACGGTCATGAAGAT
CATATCGGTGGGATTCCATTCCTATTAAAACAGATCAACGTGCCAATCTATGCCGGCCCCCTAGCCTTAGCCTTGATTAA
GGGTAAGTTAGAGGAACACGGGTTATTAAAAACAACTGAATTACACGAGATCAATGAAGATACGGTGCTCAAGTTCCGTA
AGACGCGTGTCTCGTTCTTCAGAACGACCCATTCAATTCCTGATACACTGGGAATCGCCGTTCAAACACCACCTGGCACG
ATTGTAGAGACCGGAGACTATAAGTTCGATCTGACACCAATTACTAACCAGCCGCCGAACCTTCAAAAGATGGCGCATCT
CGGTGAACGTGGTGTCTTGGCGCTAATGTCAGACAGTACTAACGCCGAACGGCCAATCTGGACGAAATCTGAACGGTGGG
TGGCGCAATCAATTCGCCACATTTTTGAACAAGTTGAAGGTCGAATCATCTTTGCTACCTTCGCTTCTAACATCTCGCGG
ATTCAAGAGGCAGCGCAGGTCGCCCTTGAACATCATCGTAAAATCGCGGTATTTGGGCGTAGTATGGAAGCAGCTATCGT
CAACGGACGTGAATTAGGCTATTTAAACATCCCTGACGAAGCCATCGTTGATGCTAACGATTTACGCTCCCTCCCCGCCA
ATAAAGTCATGATTCTATGTACTGGTTCCCAAGGTGAACCAATGGCGGCGCTCTCGCGAATTGCCAACGGTACTCATCGC
CAGATTTCTATTGAACCTGGCGACACCGTGATCTTTTCAAGTTCACCGATTCCTGGTAATACGTTAAGTGTTAACCACGT
AATCAACGAACTCGAAGAAGCCGGTGCGAATGTCATTCATGGTAAAGTCAATAACATTCATACATCTGGTCATGGTGGTC
AGGAAGAACAGAAACTGATGCTACGGCTGATGAAACCAAAGTTCTTTATGCCGATTCACGGTGAATATCGAATGTTGAAG
ATTCACACCGAACTGGCCGAACAATGTGGTGTGCCACTAGACCATAGTTTTATCCTTGAAAATGGTGATGTGCTTGCCTT
TACGAAGGATTCTGCCCGGGTTGCCGGTCACTTTGGTGCTGGCGATGTCTATGTTGATGGTTCTGGAATCGGCGATATTG
GCAACGTTGTGTTACGCGACCGCCAAGTGCTTTCGGAAGAAGGCTTGGTTGTTGTCGTTGCTACCATCAATTTGAACAAA
AAAGAGATCCAAGCTGGTCCAGATATTCTGTCACGTGGTTTTGTCTACATGCGCGAGTCTGGCGATCTAATTAACGAAGC
CCGGCGGCATGTCTTCCGTACGATCCGGCGCAAAATGAAGTCGGATAAAGCAACCGAAGCGACTATCCGCAATGCGATTA
TTGACGACCTGCAGAGTTTCTTATTTGATAAGACTGAACGGCACCCAATGATTCTGCCAATGTTGATTATGAACTAA

Upstream 100 bases:

>100_bases
ATAATACAGATCACAACATCGAATTTATCGAACCGTTAGAAGGTAACTTCTTGGATTACGAACAAAAAAATCCAGAGTAT
CACCTAACGGAGTTCAATAA

Downstream 100 bases:

>100_bases
CTACTTGCCGGAACGATCTCGTTCCGGTTTTTTATGGTGTAAGACTGATATGAGGTGTAATTTTGATTGCTGAATACTAC
ATTATTATTAATGAACTGGC

Product: metallo-beta-lactamase superfamily protein (putative)

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MKRLNVKNNETAVFAVGGLGEIGKNTYGIQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYLVANQQKIKALVITHGHED
HIGGIPFLLKQINVPIYAGPLALALIKGKLEEHGLLKTTELHEINEDTVLKFRKTRVSFFRTTHSIPDTLGIAVQTPPGT
IVETGDYKFDLTPITNQPPNLQKMAHLGERGVLALMSDSTNAERPIWTKSERWVAQSIRHIFEQVEGRIIFATFASNISR
IQEAAQVALEHHRKIAVFGRSMEAAIVNGRELGYLNIPDEAIVDANDLRSLPANKVMILCTGSQGEPMAALSRIANGTHR
QISIEPGDTVIFSSSPIPGNTLSVNHVINELEEAGANVIHGKVNNIHTSGHGGQEEQKLMLRLMKPKFFMPIHGEYRMLK
IHTELAEQCGVPLDHSFILENGDVLAFTKDSARVAGHFGAGDVYVDGSGIGDIGNVVLRDRQVLSEEGLVVVVATINLNK
KEIQAGPDILSRGFVYMRESGDLINEARRHVFRTIRRKMKSDKATEATIRNAIIDDLQSFLFDKTERHPMILPMLIMN

Sequences:

>Translated_558_residues
MKRLNVKNNETAVFAVGGLGEIGKNTYGIQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYLVANQQKIKALVITHGHED
HIGGIPFLLKQINVPIYAGPLALALIKGKLEEHGLLKTTELHEINEDTVLKFRKTRVSFFRTTHSIPDTLGIAVQTPPGT
IVETGDYKFDLTPITNQPPNLQKMAHLGERGVLALMSDSTNAERPIWTKSERWVAQSIRHIFEQVEGRIIFATFASNISR
IQEAAQVALEHHRKIAVFGRSMEAAIVNGRELGYLNIPDEAIVDANDLRSLPANKVMILCTGSQGEPMAALSRIANGTHR
QISIEPGDTVIFSSSPIPGNTLSVNHVINELEEAGANVIHGKVNNIHTSGHGGQEEQKLMLRLMKPKFFMPIHGEYRMLK
IHTELAEQCGVPLDHSFILENGDVLAFTKDSARVAGHFGAGDVYVDGSGIGDIGNVVLRDRQVLSEEGLVVVVATINLNK
KEIQAGPDILSRGFVYMRESGDLINEARRHVFRTIRRKMKSDKATEATIRNAIIDDLQSFLFDKTERHPMILPMLIMN
>Mature_558_residues
MKRLNVKNNETAVFAVGGLGEIGKNTYGIQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYLVANQQKIKALVITHGHED
HIGGIPFLLKQINVPIYAGPLALALIKGKLEEHGLLKTTELHEINEDTVLKFRKTRVSFFRTTHSIPDTLGIAVQTPPGT
IVETGDYKFDLTPITNQPPNLQKMAHLGERGVLALMSDSTNAERPIWTKSERWVAQSIRHIFEQVEGRIIFATFASNISR
IQEAAQVALEHHRKIAVFGRSMEAAIVNGRELGYLNIPDEAIVDANDLRSLPANKVMILCTGSQGEPMAALSRIANGTHR
QISIEPGDTVIFSSSPIPGNTLSVNHVINELEEAGANVIHGKVNNIHTSGHGGQEEQKLMLRLMKPKFFMPIHGEYRMLK
IHTELAEQCGVPLDHSFILENGDVLAFTKDSARVAGHFGAGDVYVDGSGIGDIGNVVLRDRQVLSEEGLVVVVATINLNK
KEIQAGPDILSRGFVYMRESGDLINEARRHVFRTIRRKMKSDKATEATIRNAIIDDLQSFLFDKTERHPMILPMLIMN

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61808; Mature: 61808

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLNVKNNETAVFAVGGLGEIGKNTYGIQFQDEIILIDAGIKFPEDELLGIDYVIPDYS
CCCCCCCCCCEEEEEECCCCCCCCCEECEEEECCEEEEECCCCCCCHHEEEEEEECCCCC
YLVANQQKIKALVITHGHEDHIGGIPFLLKQINVPIYAGPLALALIKGKLEEHGLLKTTE
EEECCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCEEEEH
LHEINEDTVLKFRKTRVSFFRTTHSIPDTLGIAVQTPPGTIVETGDYKFDLTPITNQPPN
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCEEEEEEECCCCCCC
LQKMAHLGERGVLALMSDSTNAERPIWTKSERWVAQSIRHIFEQVEGRIIFATFASNISR
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHH
IQEAAQVALEHHRKIAVFGRSMEAAIVNGRELGYLNIPDEAIVDANDLRSLPANKVMILC
HHHHHHHHHHHCCEEEEECCCEEEEEECCCEEEEEECCHHHHCCHHHHHCCCCCCEEEEE
TGSQGEPMAALSRIANGTHRQISIEPGDTVIFSSSPIPGNTLSVNHVINELEEAGANVIH
ECCCCCHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHCCCEEEE
GKVNNIHTSGHGGQEEQKLMLRLMKPKFFMPIHGEYRMLKIHTELAEQCGVPLDHSFILE
CCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEEHHHHHHCCCCCCCEEEEE
NGDVLAFTKDSARVAGHFGAGDVYVDGSGIGDIGNVVLRDRQVLSEEGLVVVVATINLNK
CCCEEEEECCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCH
KEIQAGPDILSRGFVYMRESGDLINEARRHVFRTIRRKMKSDKATEATIRNAIIDDLQSF
HHHHCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LFDKTERHPMILPMLIMN
HHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKRLNVKNNETAVFAVGGLGEIGKNTYGIQFQDEIILIDAGIKFPEDELLGIDYVIPDYS
CCCCCCCCCCEEEEEECCCCCCCCCEECEEEECCEEEEECCCCCCCHHEEEEEEECCCCC
YLVANQQKIKALVITHGHEDHIGGIPFLLKQINVPIYAGPLALALIKGKLEEHGLLKTTE
EEECCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCEEEEH
LHEINEDTVLKFRKTRVSFFRTTHSIPDTLGIAVQTPPGTIVETGDYKFDLTPITNQPPN
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCEEEEEEECCCCCCC
LQKMAHLGERGVLALMSDSTNAERPIWTKSERWVAQSIRHIFEQVEGRIIFATFASNISR
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHH
IQEAAQVALEHHRKIAVFGRSMEAAIVNGRELGYLNIPDEAIVDANDLRSLPANKVMILC
HHHHHHHHHHHCCEEEEECCCEEEEEECCCEEEEEECCHHHHCCHHHHHCCCCCCEEEEE
TGSQGEPMAALSRIANGTHRQISIEPGDTVIFSSSPIPGNTLSVNHVINELEEAGANVIH
ECCCCCHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHCCCEEEE
GKVNNIHTSGHGGQEEQKLMLRLMKPKFFMPIHGEYRMLKIHTELAEQCGVPLDHSFILE
CCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEEHHHHHHCCCCCCCEEEEE
NGDVLAFTKDSARVAGHFGAGDVYVDGSGIGDIGNVVLRDRQVLSEEGLVVVVATINLNK
CCCEEEEECCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCH
KEIQAGPDILSRGFVYMRESGDLINEARRHVFRTIRRKMKSDKATEATIRNAIIDDLQSF
HHHHCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LFDKTERHPMILPMLIMN
HHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]