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The map label for this gene is glnPH1 [H]

Identifier: 28377653

GI number: 28377653

Start: 743457

End: 744893

Strand: Direct

Name: glnPH1 [H]

Synonym: lp_0802

Alternate gene names: 28377653

Gene position: 743457-744893 (Clockwise)

Preceding gene: 28377652

Following gene: 28377654

Centisome position: 22.47

GC content: 44.26

Gene sequence:

>1437_bases
ATGAGAAAGTGGCAAGTTGCAGTTGTCATGTTGTTAGCAGCGTTGGGAAGCTGGTTTGCGATTGGTACCCAGGCCCAGGC
TAAGACGTATACGATTGCGACGGATACAACCTTTGCGCCGTTCGAATTTCAGGTCAAGGGTGGTAAGTATAAGGGAATTG
ATATCGATATTTTGAAGGCAATTGCCAAAAAAGAAAATTTCAATTATAAATTAAAAGCGCTTAGTTTCGGTGCCGCTGTT
CAGCAGCTTAGTGCGAACCAAGTTGATGGGGTAATTGCCGGAATGAATATTACGGCTGAACGGAAGCAGACGTTTGATTT
TTCAGACGCTTACTATACTTCCGGGGTGGTCATGGCTGTGGCTAAAGATAGCAAGGTCAAAACGTTTAAAGATTTGAAAG
GTAAGACCGTGGCCCTTAAAACGGGGACAGCTGGTGCAACCTATGCGAAGTCGATTCAGAGCAAGTACGGCTTCAAAATC
AAGTATTTCAATGACTCGAATAACATGTATAACGATGTTAAGGTCGGCAACTCGGCTGCTTGTTTTGAAGACTATCCCGT
GATGTCATACGGAATCAAGAATGGCATCGCGTTGAAGATTGTTTCCAAGCAACACGAGGCCGGCGACTATGGTTTCGCGG
TTAAAAAAGGTAAAAACGCGGAACTGTTAAAGAAATTTAACGCGGGTCTCAAAGCGATTAAAGCGGATGGTACTTACAAA
AAGATCATTAACAAGTACTTGAAGTCTAGTGAAGCATCCTTAACGGGTGAAGACGCAAGCAGTCGGACCTTTATTGGCTT
GTTCACCCAAAACTTGGATACGATTGGCTCAGGGTTATGGATGACCTTGGAATTAACGGTTATTTCAATTATTTTAGCGA
CGATCCTCGGACTGATTCTTGGGGTCTTAGGCGTTATGCCGGGTAAACTTGGTCCGGCCATCTCCAGTACGATTATTTAT
ATTTTCCGTGGGATGCCATTGATGGTGTTGGCCTTCTTTATTTACATTGGGTTCCCAGACCTGATTGGGCACGGCTTTAA
GATTCCCGCGTTCGTTGCTGGGATGATTACGTTGATGCTTAACGAAGGGGCCTATACCGGAGCCTTTGTTAAGGGGGGCT
TTGCCGCGGTCGACGATGGTCAAATGGAGGCGGCCCGTTCACTCGGGTTGCCATACTGGACGGCCATGCGCAAGGTTATT
ATGCCCCAAGGAATTCGAATCATGATTCCGTCGTTCATTAACCAATTTATTATCACGTTGAAGGATACGTCAATTCTATC
CGTTATTGGGATCGTTGAATTAACCCAAACTGGGACGTTGATTATTGCCCGGAACTTCGAAGGCTTTAAGATTTGGTTAA
TGATTGCGATTATTTATCTGATTATCATTACGTTATTAACATGGTTATCTAACTGGGTTCAAAGGAGGATTAACTAA

Upstream 100 bases:

>100_bases
AAATTAAAAAAGTATAAAAAATTGAATTGGTCGCGAGACATGAATTATGTTATATTTTATGACATACCGTTCCGGTAAAC
TATTTGAGGAGGTCTCGTCG

Downstream 100 bases:

>100_bases
GCATGGCGAAAGTAATTGTCAAAGATTTGCATAAAAGCTACGGCGATAACGAAGTCTTGAAAGGGTTAAACCTTGAAGTT
CAAGATAACGAAGTAGTCTG

Product: glutamine ABC transporter, substrate binding and permease protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRKWQVAVVMLLAALGSWFAIGTQAQAKTYTIATDTTFAPFEFQVKGGKYKGIDIDILKAIAKKENFNYKLKALSFGAAV
QQLSANQVDGVIAGMNITAERKQTFDFSDAYYTSGVVMAVAKDSKVKTFKDLKGKTVALKTGTAGATYAKSIQSKYGFKI
KYFNDSNNMYNDVKVGNSAACFEDYPVMSYGIKNGIALKIVSKQHEAGDYGFAVKKGKNAELLKKFNAGLKAIKADGTYK
KIINKYLKSSEASLTGEDASSRTFIGLFTQNLDTIGSGLWMTLELTVISIILATILGLILGVLGVMPGKLGPAISSTIIY
IFRGMPLMVLAFFIYIGFPDLIGHGFKIPAFVAGMITLMLNEGAYTGAFVKGGFAAVDDGQMEAARSLGLPYWTAMRKVI
MPQGIRIMIPSFINQFIITLKDTSILSVIGIVELTQTGTLIIARNFEGFKIWLMIAIIYLIIITLLTWLSNWVQRRIN

Sequences:

>Translated_478_residues
MRKWQVAVVMLLAALGSWFAIGTQAQAKTYTIATDTTFAPFEFQVKGGKYKGIDIDILKAIAKKENFNYKLKALSFGAAV
QQLSANQVDGVIAGMNITAERKQTFDFSDAYYTSGVVMAVAKDSKVKTFKDLKGKTVALKTGTAGATYAKSIQSKYGFKI
KYFNDSNNMYNDVKVGNSAACFEDYPVMSYGIKNGIALKIVSKQHEAGDYGFAVKKGKNAELLKKFNAGLKAIKADGTYK
KIINKYLKSSEASLTGEDASSRTFIGLFTQNLDTIGSGLWMTLELTVISIILATILGLILGVLGVMPGKLGPAISSTIIY
IFRGMPLMVLAFFIYIGFPDLIGHGFKIPAFVAGMITLMLNEGAYTGAFVKGGFAAVDDGQMEAARSLGLPYWTAMRKVI
MPQGIRIMIPSFINQFIITLKDTSILSVIGIVELTQTGTLIIARNFEGFKIWLMIAIIYLIIITLLTWLSNWVQRRIN
>Mature_478_residues
MRKWQVAVVMLLAALGSWFAIGTQAQAKTYTIATDTTFAPFEFQVKGGKYKGIDIDILKAIAKKENFNYKLKALSFGAAV
QQLSANQVDGVIAGMNITAERKQTFDFSDAYYTSGVVMAVAKDSKVKTFKDLKGKTVALKTGTAGATYAKSIQSKYGFKI
KYFNDSNNMYNDVKVGNSAACFEDYPVMSYGIKNGIALKIVSKQHEAGDYGFAVKKGKNAELLKKFNAGLKAIKADGTYK
KIINKYLKSSEASLTGEDASSRTFIGLFTQNLDTIGSGLWMTLELTVISIILATILGLILGVLGVMPGKLGPAISSTIIY
IFRGMPLMVLAFFIYIGFPDLIGHGFKIPAFVAGMITLMLNEGAYTGAFVKGGFAAVDDGQMEAARSLGLPYWTAMRKVI
MPQGIRIMIPSFINQFIITLKDTSILSVIGIVELTQTGTLIIARNFEGFKIWLMIAIIYLIIITLLTWLSNWVQRRIN

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=241, Percent_Identity=41.49377593361, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1787030, Length=198, Percent_Identity=35.8585858585859, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=33.6448598130841, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=33.1967213114754, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=30, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=31.0204081632653, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=29.4573643410853, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788228, Length=221, Percent_Identity=28.5067873303167, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786873, Length=213, Percent_Identity=23.943661971831, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082239, Length=171, Percent_Identity=30.9941520467836, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787087, Length=219, Percent_Identity=30.1369863013699, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788646, Length=213, Percent_Identity=30.0469483568075, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786874, Length=223, Percent_Identity=27.8026905829596, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788645, Length=207, Percent_Identity=29.4685990338164, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI48994930, Length=164, Percent_Identity=31.7073170731707, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787086, Length=215, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 52271; Mature: 52271

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKWQVAVVMLLAALGSWFAIGTQAQAKTYTIATDTTFAPFEFQVKGGKYKGIDIDILKA
CCCHHHHHHHHHHHHCCHHEECCCCCCEEEEEEECCEECCEEEEECCCEECCCCHHHHHH
IAKKENFNYKLKALSFGAAVQQLSANQVDGVIAGMNITAERKQTFDFSDAYYTSGVVMAV
HHHCCCCCEEEEEEEHHHHHHHHCCCCCCCEEECCCEEECCCCCCCCCCHHHCCCEEEEE
AKDSKVKTFKDLKGKTVALKTGTAGATYAKSIQSKYGFKIKYFNDSNNMYNDVKVGNSAA
ECCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCCC
CFEDYPVMSYGIKNGIALKIVSKQHEAGDYGFAVKKGKNAELLKKFNAGLKAIKADGTYK
CCCCCCCHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHCCCCEEEECCCHHH
KIINKYLKSSEASLTGEDASSRTFIGLFTQNLDTIGSGLWMTLELTVISIILATILGLIL
HHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCHHHHCCCEEEEHHHHHHHHHHHHHHHHHH
GVLGVMPGKLGPAISSTIIYIFRGMPLMVLAFFIYIGFPDLIGHGFKIPAFVAGMITLML
HHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHC
NEGAYTGAFVKGGFAAVDDGQMEAARSLGLPYWTAMRKVIMPQGIRIMIPSFINQFIITL
CCCCCCCCEECCCEEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHEEEE
KDTSILSVIGIVELTQTGTLIIARNFEGFKIWLMIAIIYLIIITLLTWLSNWVQRRIN
CCCHHHHHHHHHEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKWQVAVVMLLAALGSWFAIGTQAQAKTYTIATDTTFAPFEFQVKGGKYKGIDIDILKA
CCCHHHHHHHHHHHHCCHHEECCCCCCEEEEEEECCEECCEEEEECCCEECCCCHHHHHH
IAKKENFNYKLKALSFGAAVQQLSANQVDGVIAGMNITAERKQTFDFSDAYYTSGVVMAV
HHHCCCCCEEEEEEEHHHHHHHHCCCCCCCEEECCCEEECCCCCCCCCCHHHCCCEEEEE
AKDSKVKTFKDLKGKTVALKTGTAGATYAKSIQSKYGFKIKYFNDSNNMYNDVKVGNSAA
ECCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCCC
CFEDYPVMSYGIKNGIALKIVSKQHEAGDYGFAVKKGKNAELLKKFNAGLKAIKADGTYK
CCCCCCCHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHCCCCEEEECCCHHH
KIINKYLKSSEASLTGEDASSRTFIGLFTQNLDTIGSGLWMTLELTVISIILATILGLIL
HHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCHHHHCCCEEEEHHHHHHHHHHHHHHHHHH
GVLGVMPGKLGPAISSTIIYIFRGMPLMVLAFFIYIGFPDLIGHGFKIPAFVAGMITLML
HHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHC
NEGAYTGAFVKGGFAAVDDGQMEAARSLGLPYWTAMRKVIMPQGIRIMIPSFINQFIITL
CCCCCCCCEECCCEEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHEEEE
KDTSILSVIGIVELTQTGTLIIARNFEGFKIWLMIAIIYLIIITLLTWLSNWVQRRIN
CCCHHHHHHHHHEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]