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The map label for this gene is rpoN [H]

Identifier: 28377640

GI number: 28377640

Start: 722327

End: 723664

Strand: Direct

Name: rpoN [H]

Synonym: lp_0787

Alternate gene names: 28377640

Gene position: 722327-723664 (Clockwise)

Preceding gene: 28377637

Following gene: 28377641

Centisome position: 21.83

GC content: 44.02

Gene sequence:

>1338_bases
ATGGCACTGGGACCAGGATTTCGCCAGCAACAGCGGCAATCACAGAAGTTAGCGATGACCCAGCGACTACAACAATCCAT
TCAGATGTTGCAGTTTAATGTGGAAGAACTAAGAGATTTTCTAACGCAAAAAGCATTAGAAAACCCATTGATCGATGTGG
ATACAAATTGGAATAATAATCACGCCAGTTTGAACGCAGCCAAAAATGTAACCACTAAGGCGGATTTCATTGAGCGTGTG
TCGACCTCTAATCAGCATTCGTTATTTGAATACTTGTTGGATCAGATTCACCTAACGATGCGTGATACCCATTTGCGTCA
GATTGTCCTTTATTTAATTGAGTACGTTGACGTGAATGGTTATTTGCGGATTGATGAGAGGCAGGTTCGGCAAGAAACTA
AGGCAACACCAATTGAGTTGTTGGATGCAATTACCTTATTACAACAACTTGATCCGCCGGGTGTTGGGGCCCGGAGCTTG
CAAGAAGCGCTGATGTTGCAGACAGAAAATGATGACCATGCACCGAACTTAGCCTATATTGTCTTAGAAGAAAGTTTTGA
CGAGCTGGTTAATCGACAATGGGACTTGTTGGCTAAGAAGTATCAGGTGACGCTTGCGGACATTTCAAGTATTTATGATT
ATGTGCGAACACTAACACCCGTACCCGGTGCGGCAATCGGGCAGGAGACGACCGGGTACATCTTTCCCGATTTAGTGGTG
ACGAATCAGGGAGGCCAGCTGGCATTGCGAACCGCATCCATGGCTCAACCGGTGGTTAAATTCCGCAGTCGGTATTATCA
GCAGATGGGGCAGCATGATGATCAGGAAGTTACGGAATATTTGAAAGAAAAGAAAAGTGAGTATGACTGGATTGCTAACA
GTTTGCAGCAACGCGAGGCAACCATTTTTCGAGTGGGAACGGCCATTATCGAGCGTCAAGCAGACTTCTTCTTGGAAAAA
ACGACGGATTTACGGCCACTATTGTTACGAGATGTTGCCCAACAGTTAAAAGTGCATGAATCAACAATCAGTCGAAGTAT
TAATGGAAAGTACATTCAAACGGACTTCGGTATGTTTGAACTTAAACGTTTCTTTACCAAAGCGGTCAGTAAACGTCCCA
CTGGTGGCAAAATCGTTTCGGCCGACAGTGTTCAACACCGAATTGTGACCTTGATCGAACAGGAAAATAAAGAAAAACCG
CTTTCAGACCAAAAAATTGTTCAGATTCTTAATGCGGAAAATGTCGAACTTTCTCGGCGGACAGTGGCGAAATATCGCGA
AAATCTTAATATTCCGTCGTCATCAAGGCGAAAACAATACTTGAGAACCGAACGATAA

Upstream 100 bases:

>100_bases
CCATTACACCACGGGCGCCTAAACAACATATTATATTCTAGCGAATAATGGATAAAAACACAAGGGTTTTACATAAAAAT
TTATTCAGGGAGGTATTTTT

Downstream 100 bases:

>100_bases
TTTAACGCGCCATTTGGACCAGTCGGTTATAAGTTTAATTGGGGTTTCATTTGCAACCACCTTATGAACCTGTTATAATT
CATGTTGTGGTAAGAGAGGT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MALGPGFRQQQRQSQKLAMTQRLQQSIQMLQFNVEELRDFLTQKALENPLIDVDTNWNNNHASLNAAKNVTTKADFIERV
STSNQHSLFEYLLDQIHLTMRDTHLRQIVLYLIEYVDVNGYLRIDERQVRQETKATPIELLDAITLLQQLDPPGVGARSL
QEALMLQTENDDHAPNLAYIVLEESFDELVNRQWDLLAKKYQVTLADISSIYDYVRTLTPVPGAAIGQETTGYIFPDLVV
TNQGGQLALRTASMAQPVVKFRSRYYQQMGQHDDQEVTEYLKEKKSEYDWIANSLQQREATIFRVGTAIIERQADFFLEK
TTDLRPLLLRDVAQQLKVHESTISRSINGKYIQTDFGMFELKRFFTKAVSKRPTGGKIVSADSVQHRIVTLIEQENKEKP
LSDQKIVQILNAENVELSRRTVAKYRENLNIPSSSRRKQYLRTER

Sequences:

>Translated_445_residues
MALGPGFRQQQRQSQKLAMTQRLQQSIQMLQFNVEELRDFLTQKALENPLIDVDTNWNNNHASLNAAKNVTTKADFIERV
STSNQHSLFEYLLDQIHLTMRDTHLRQIVLYLIEYVDVNGYLRIDERQVRQETKATPIELLDAITLLQQLDPPGVGARSL
QEALMLQTENDDHAPNLAYIVLEESFDELVNRQWDLLAKKYQVTLADISSIYDYVRTLTPVPGAAIGQETTGYIFPDLVV
TNQGGQLALRTASMAQPVVKFRSRYYQQMGQHDDQEVTEYLKEKKSEYDWIANSLQQREATIFRVGTAIIERQADFFLEK
TTDLRPLLLRDVAQQLKVHESTISRSINGKYIQTDFGMFELKRFFTKAVSKRPTGGKIVSADSVQHRIVTLIEQENKEKP
LSDQKIVQILNAENVELSRRTVAKYRENLNIPSSSRRKQYLRTER
>Mature_444_residues
ALGPGFRQQQRQSQKLAMTQRLQQSIQMLQFNVEELRDFLTQKALENPLIDVDTNWNNNHASLNAAKNVTTKADFIERVS
TSNQHSLFEYLLDQIHLTMRDTHLRQIVLYLIEYVDVNGYLRIDERQVRQETKATPIELLDAITLLQQLDPPGVGARSLQ
EALMLQTENDDHAPNLAYIVLEESFDELVNRQWDLLAKKYQVTLADISSIYDYVRTLTPVPGAAIGQETTGYIFPDLVVT
NQGGQLALRTASMAQPVVKFRSRYYQQMGQHDDQEVTEYLKEKKSEYDWIANSLQQREATIFRVGTAIIERQADFFLEKT
TDLRPLLLRDVAQQLKVHESTISRSINGKYIQTDFGMFELKRFFTKAVSKRPTGGKIVSADSVQHRIVTLIEQENKEKPL
SDQKIVQILNAENVELSRRTVAKYRENLNIPSSSRRKQYLRTER

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=474, Percent_Identity=28.9029535864979, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51366; Mature: 51235

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGPGFRQQQRQSQKLAMTQRLQQSIQMLQFNVEELRDFLTQKALENPLIDVDTNWNNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCCCC
HASLNAAKNVTTKADFIERVSTSNQHSLFEYLLDQIHLTMRDTHLRQIVLYLIEYVDVNG
CCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YLRIDERQVRQETKATPIELLDAITLLQQLDPPGVGARSLQEALMLQTENDDHAPNLAYI
EEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEE
VLEESFDELVNRQWDLLAKKYQVTLADISSIYDYVRTLTPVPGAAIGQETTGYIFPDLVV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
TNQGGQLALRTASMAQPVVKFRSRYYQQMGQHDDQEVTEYLKEKKSEYDWIANSLQQREA
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIFRVGTAIIERQADFFLEKTTDLRPLLLRDVAQQLKVHESTISRSINGKYIQTDFGMFE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHH
LKRFFTKAVSKRPTGGKIVSADSVQHRIVTLIEQENKEKPLSDQKIVQILNAENVELSRR
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHH
TVAKYRENLNIPSSSRRKQYLRTER
HHHHHHHCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ALGPGFRQQQRQSQKLAMTQRLQQSIQMLQFNVEELRDFLTQKALENPLIDVDTNWNNN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCCCC
HASLNAAKNVTTKADFIERVSTSNQHSLFEYLLDQIHLTMRDTHLRQIVLYLIEYVDVNG
CCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YLRIDERQVRQETKATPIELLDAITLLQQLDPPGVGARSLQEALMLQTENDDHAPNLAYI
EEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEE
VLEESFDELVNRQWDLLAKKYQVTLADISSIYDYVRTLTPVPGAAIGQETTGYIFPDLVV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
TNQGGQLALRTASMAQPVVKFRSRYYQQMGQHDDQEVTEYLKEKKSEYDWIANSLQQREA
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIFRVGTAIIERQADFFLEKTTDLRPLLLRDVAQQLKVHESTISRSINGKYIQTDFGMFE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHH
LKRFFTKAVSKRPTGGKIVSADSVQHRIVTLIEQENKEKPLSDQKIVQILNAENVELSRR
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHH
TVAKYRENLNIPSSSRRKQYLRTER
HHHHHHHCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]