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The map label for this gene is oppA [H]

Identifier: 28377637

GI number: 28377637

Start: 717767

End: 719419

Strand: Direct

Name: oppA [H]

Synonym: lp_0783

Alternate gene names: 28377637

Gene position: 717767-719419 (Clockwise)

Preceding gene: 28377636

Following gene: 28377640

Centisome position: 21.7

GC content: 40.71

Gene sequence:

>1653_bases
TTGAAGAAGCGATCAGTGTTAGCAGTGGTAATGAGCCTAGTATTAGTGACGGTGCTGGCTGCGTGTGGGAAGCACAATAG
CCAGTCTTCTGGTAATGGCAAGTATGCAAGTAGTCAGGTTTTAAACTTGTCGTACCCTAGTTCATTGGATTCAATCGATA
TTTCCAATATGTCGGGATATGGCAGTACGGGTAATATTTTTGAAAGCTTATACCGGTTAGGAAAGAATGGTAGCATTACA
CCGGGATTAGCTAAGTCCACTAAGGTTTCAAAGGATGGCAAGACGTATACCTTTACGATTCGCAATGCGAAGTGGAGCGA
TGGTTCGAAGATAACCGCGCAAGACTTTGTCTATTCTTGGAAACGGACCGTCACCCCAGCAACTAAGTCACAGTATGCCT
ACCTCTTCTCAGGGGTGAAGAATGCGGATGAAATTGTCGCTGGGAAAAAGTCACCAAGTACATTGGGCGTCAAGGCGCAA
GGCGAACATACTTTTATTGTGACTCTTGATAAGCCAATTACTTATTTTAAGAAGTTAATGACCTACCCGTTGTTTGGACC
AATTAGTGAGAAAGCGGTCAAAAAGTGGGGTAACAAATATGCCACTAAGGCTCAGTACATGCTCTACAGTGGGCCGTTTA
AGTTGACTGGCTGGACTGGAACTAATAATAGCTGGCAGTTTGTAAAAAATAATCAGTACTGGGATAAGAAAGCTGTCCAC
CTACAAAAGATCAACTACACGGTCAATGAGAGTACTACGACAACATTGAACTTGTTCCAAGAAAAGAAACTTGATCTAAC
GCAATTAGCGAGTGAACAAGTTAAGAATATGAAGAGCAGTTCTGACTATACCACGTACCCTTATTCTATCACCGCGTTTC
TGGTTTATAATTTCCAAGACAGTAATGCGACAATCAAAAAGGCGCTCAATAACGCTAAGATTCGTCAAGCAATTTCATTG
TCGATCAATCGCAAGACGTTAGTAAAGAATGTGATTGGTGACGCATCAACGGTCTCAAAGACGTTTGTTCCTCAGGATTT
AGTTAAAGACGCTAAAACTGGTAAGGATTTTGCAGATGAATCAACAGTTAAGAATTCAACGTCATATAACAAAGCGCTGG
CACAAAAGTTATGGAAACAAGGCCTTAAGGAAACGGGAATTAAGAAATTGTCCATTCAGTTATTAGCTTCTAATGACGAA
CCTAATAAGCCGATTTCACAATACTTGAAATCAGCATTAGAAAAGAATCTCGATGGTTTGACGGTCAACTTATCCAACAT
TCCATCAAAGGTAGCTTCAAGTCGTGCTCAAAGTGGTGATTTTGACCTTTATCTCAGTGGTTGGGGTGCTGACTTCAACG
ACCCAATCTCGCACTTGCAAATTATGACTAATAATAGTGGTTATAATTACGGAAAATATAACAGCAGCACGTACAATGCG
TTGGTTAACAAGGCCCAAAACCAGGATGCCAATGATACTAGTGCTCGTTGGCAAGATATGATCAACGCTGAGAAGACGAT
CATGAAGGACCAAGGAATTACGCCATTGTATCAGACGGTATACTCTTATTTACAAAATCCAAAGGTAAAGGGTATTATTC
ATAATACTGCTGGGACACAGTGGAATTATAAGTATGCTTATATTGCCAAATAA

Upstream 100 bases:

>100_bases
GCTTGGAATCTGACGAGATTCTGAACAGGCGTTTTTTGTTACAACCGATGGGAATAAGTAGATCAACACATCAAAATGGG
AATGTAAAGGGGCGAGAAGT

Downstream 100 bases:

>100_bases
TTAACTGATTGAGGGACGTTCATGGTAACTGGTCGTTTAGCTGTTACCTTAGTAGGAGCGCCAGGTCCATACGTTGCCTT
GATCAAAACAAAATAGCGGT

Product: lipoprotein precursor, peptide binding protein OppA-like protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKKRSVLAVVMSLVLVTVLAACGKHNSQSSGNGKYASSQVLNLSYPSSLDSIDISNMSGYGSTGNIFESLYRLGKNGSIT
PGLAKSTKVSKDGKTYTFTIRNAKWSDGSKITAQDFVYSWKRTVTPATKSQYAYLFSGVKNADEIVAGKKSPSTLGVKAQ
GEHTFIVTLDKPITYFKKLMTYPLFGPISEKAVKKWGNKYATKAQYMLYSGPFKLTGWTGTNNSWQFVKNNQYWDKKAVH
LQKINYTVNESTTTTLNLFQEKKLDLTQLASEQVKNMKSSSDYTTYPYSITAFLVYNFQDSNATIKKALNNAKIRQAISL
SINRKTLVKNVIGDASTVSKTFVPQDLVKDAKTGKDFADESTVKNSTSYNKALAQKLWKQGLKETGIKKLSIQLLASNDE
PNKPISQYLKSALEKNLDGLTVNLSNIPSKVASSRAQSGDFDLYLSGWGADFNDPISHLQIMTNNSGYNYGKYNSSTYNA
LVNKAQNQDANDTSARWQDMINAEKTIMKDQGITPLYQTVYSYLQNPKVKGIIHNTAGTQWNYKYAYIAK

Sequences:

>Translated_550_residues
MKKRSVLAVVMSLVLVTVLAACGKHNSQSSGNGKYASSQVLNLSYPSSLDSIDISNMSGYGSTGNIFESLYRLGKNGSIT
PGLAKSTKVSKDGKTYTFTIRNAKWSDGSKITAQDFVYSWKRTVTPATKSQYAYLFSGVKNADEIVAGKKSPSTLGVKAQ
GEHTFIVTLDKPITYFKKLMTYPLFGPISEKAVKKWGNKYATKAQYMLYSGPFKLTGWTGTNNSWQFVKNNQYWDKKAVH
LQKINYTVNESTTTTLNLFQEKKLDLTQLASEQVKNMKSSSDYTTYPYSITAFLVYNFQDSNATIKKALNNAKIRQAISL
SINRKTLVKNVIGDASTVSKTFVPQDLVKDAKTGKDFADESTVKNSTSYNKALAQKLWKQGLKETGIKKLSIQLLASNDE
PNKPISQYLKSALEKNLDGLTVNLSNIPSKVASSRAQSGDFDLYLSGWGADFNDPISHLQIMTNNSGYNYGKYNSSTYNA
LVNKAQNQDANDTSARWQDMINAEKTIMKDQGITPLYQTVYSYLQNPKVKGIIHNTAGTQWNYKYAYIAK
>Mature_550_residues
MKKRSVLAVVMSLVLVTVLAACGKHNSQSSGNGKYASSQVLNLSYPSSLDSIDISNMSGYGSTGNIFESLYRLGKNGSIT
PGLAKSTKVSKDGKTYTFTIRNAKWSDGSKITAQDFVYSWKRTVTPATKSQYAYLFSGVKNADEIVAGKKSPSTLGVKAQ
GEHTFIVTLDKPITYFKKLMTYPLFGPISEKAVKKWGNKYATKAQYMLYSGPFKLTGWTGTNNSWQFVKNNQYWDKKAVH
LQKINYTVNESTTTTLNLFQEKKLDLTQLASEQVKNMKSSSDYTTYPYSITAFLVYNFQDSNATIKKALNNAKIRQAISL
SINRKTLVKNVIGDASTVSKTFVPQDLVKDAKTGKDFADESTVKNSTSYNKALAQKLWKQGLKETGIKKLSIQLLASNDE
PNKPISQYLKSALEKNLDGLTVNLSNIPSKVASSRAQSGDFDLYLSGWGADFNDPISHLQIMTNNSGYNYGKYNSSTYNA
LVNKAQNQDANDTSARWQDMINAEKTIMKDQGITPLYQTVYSYLQNPKVKGIIHNTAGTQWNYKYAYIAK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=30.3370786516854, Blast_Score=208, Evalue=9e-55,
Organism=Escherichia coli, GI1789397, Length=533, Percent_Identity=28.3302063789869, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081878, Length=496, Percent_Identity=30.6451612903226, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1787762, Length=480, Percent_Identity=24.7916666666667, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789966, Length=562, Percent_Identity=22.5978647686833, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787052, Length=367, Percent_Identity=22.8882833787466, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787551, Length=491, Percent_Identity=20.5702647657841, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61087; Mature: 61087

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRSVLAVVMSLVLVTVLAACGKHNSQSSGNGKYASSQVLNLSYPSSLDSIDISNMSGY
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCCC
GSTGNIFESLYRLGKNGSITPGLAKSTKVSKDGKTYTFTIRNAKWSDGSKITAQDFVYSW
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHH
KRTVTPATKSQYAYLFSGVKNADEIVAGKKSPSTLGVKAQGEHTFIVTLDKPITYFKKLM
HHHCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHH
TYPLFGPISEKAVKKWGNKYATKAQYMLYSGPFKLTGWTGTNNSWQFVKNNQYWDKKAVH
HCCCCCCCHHHHHHHHCHHHHCCEEEEEEECCEEEEEECCCCCCEEEEECCCCCCCCCEE
LQKINYTVNESTTTTLNLFQEKKLDLTQLASEQVKNMKSSSDYTTYPYSITAFLVYNFQD
EEEEEEEECCCCCEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECCEEEEEEEEEEEEC
SNATIKKALNNAKIRQAISLSINRKTLVKNVIGDASTVSKTFVPQDLVKDAKTGKDFADE
CCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCCCH
STVKNSTSYNKALAQKLWKQGLKETGIKKLSIQLLASNDEPNKPISQYLKSALEKNLDGL
HHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCE
TVNLSNIPSKVASSRAQSGDFDLYLSGWGADFNDPISHLQIMTNNSGYNYGKYNSSTYNA
EEEHHHCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHH
LVNKAQNQDANDTSARWQDMINAEKTIMKDQGITPLYQTVYSYLQNPKVKGIIHNTAGTQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
WNYKYAYIAK
CCEEEEEEEC
>Mature Secondary Structure
MKKRSVLAVVMSLVLVTVLAACGKHNSQSSGNGKYASSQVLNLSYPSSLDSIDISNMSGY
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCCC
GSTGNIFESLYRLGKNGSITPGLAKSTKVSKDGKTYTFTIRNAKWSDGSKITAQDFVYSW
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHH
KRTVTPATKSQYAYLFSGVKNADEIVAGKKSPSTLGVKAQGEHTFIVTLDKPITYFKKLM
HHHCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHH
TYPLFGPISEKAVKKWGNKYATKAQYMLYSGPFKLTGWTGTNNSWQFVKNNQYWDKKAVH
HCCCCCCCHHHHHHHHCHHHHCCEEEEEEECCEEEEEECCCCCCEEEEECCCCCCCCCEE
LQKINYTVNESTTTTLNLFQEKKLDLTQLASEQVKNMKSSSDYTTYPYSITAFLVYNFQD
EEEEEEEECCCCCEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECCEEEEEEEEEEEEC
SNATIKKALNNAKIRQAISLSINRKTLVKNVIGDASTVSKTFVPQDLVKDAKTGKDFADE
CCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCCCH
STVKNSTSYNKALAQKLWKQGLKETGIKKLSIQLLASNDEPNKPISQYLKSALEKNLDGL
HHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCE
TVNLSNIPSKVASSRAQSGDFDLYLSGWGADFNDPISHLQIMTNNSGYNYGKYNSSTYNA
EEEHHHCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHH
LVNKAQNQDANDTSARWQDMINAEKTIMKDQGITPLYQTVYSYLQNPKVKGIIHNTAGTQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
WNYKYAYIAK
CCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]