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The map label for this gene is argH

Identifier: 28377632

GI number: 28377632

Start: 712177

End: 713580

Strand: Direct

Name: argH

Synonym: lp_0776

Alternate gene names: 28377632

Gene position: 712177-713580 (Clockwise)

Preceding gene: 28377631

Following gene: 28377633

Centisome position: 21.53

GC content: 47.29

Gene sequence:

>1404_bases
ATGAGTACCCAGAAGCTATGGGGTGGCCGGTTTACCGAAACGGGTGCTAAGTATGTTGATGACTTTGGGGCATCAATTAG
CTTTGACCAGTTGCTTGCCGCAGAAGATATTGCTGGTTCACTGGCACACGTTAAAATGTTGAAGAAAACTGGTATTTTGC
CCGCCGCTGATGTTGATCAAATTGTTGCTGGTTTGGAAACCTTGGCTGAGCGTCAAAAACAAGGTGAGTTGGAATTTTCA
ACGGTCAACGAAGACATTCATATGAATATTGAGTCATTATTGACCGAAGAGATTGGGCCGGTCGCTGGGAAGTTGCACAC
GGCCCGGTCGCGGAATGACCAAGTTGCCACGGATTTCCACTTGTACTTGAAGCATCAATTACCACTAATATTGGACCGGC
TACATGAACTGGAAACAGTATTGGTCGATAAAGCGAGTGAAAACGTGGAGACGGTCATGCCGGGGTACACGCATTTGCAA
CACGCCCAACCCATCTCGTACGCGCACTACCTGTTAGCTTATTACCAAATGTTCAAGCGGGACATGGAACGCTTCGAGTT
TAACCTGAAGCATACCGATATTTCGCCATTGGGAGCCGCTGCACTTGCTGGAACGACTTTTCCAATCGACCGGGAATATA
GTGCGAAACTATTAGGATTCAGTGAAGTCTATCATAATAGTTTGGATGCCGTTTCTGACCGGGACTTCGTGCTTGAGTTT
CTCAGCAATGCTTCGATCCTGATGATGCATCTCTCACGGTTTTGTGAAGAAATTGTCAGCTGGAGCAGTTATGAATTCAA
GTACATCAGTTTGAGCGATCAGTTTTCAACGGGTAGTTCGATTATGCCGCAAAAGAAGAACCCTGACATGGCCGAATTGA
TTCGGGGCAAGTCCGGCCGGGTTTACGGCAACTTGATGGGATTGCTAACTGTCATGAAAGGGATTCCGCTCGCTTACAAT
AAGGATCTGCAAGAAGACAAAGAAGGGGCGTTTGATACGGTCACGACCGTGTTAACGAGTCTCCACGTCTTCACCGGCAT
GTTGGCAACGCTGACCGTTAATGAAGATCGGATGGCTGATGCCACGACTAACGATTTCTCAAATGCGACCGAACTAGCCG
ATTACCTTGCAAACAAAGGAATCCCATTTCGGGAGGCCCATGCCATTGTGGGGAAGCTCGTCTTGGATGGCTTGAAAAAA
CAGCGGCCGTTGCAGGATATTCCACTCAAAGAATATCAGTCAATCTCACCGCTGATCCAAGAAGATGTCTACCATGATTT
GAACGCAAAAGTTGCGGTGGAACGGCGGCATTCGCTAGGTGGTACTGGTTTTGATCAGATTAGACAAGAATTGCAACGGG
CACAAACGCAATTGTCAGCCTATCAATCTGAAACCACTGATTAA

Upstream 100 bases:

>100_bases
CAGTTGGTTTCATTAAGCTATGGGGCCTACCAACGCAAGTTAATGCCCAAGTCCAAGCGAAGGCTCAGGCCGAAGCTAAG
ACTGATAAGGCACACGCCTA

Downstream 100 bases:

>100_bases
TTAATGCTTATCAATTTGACCATTGATATTATTTTTAAAGATGACCTCTAAGTGGCGGAGCCTCGGCATTTAGAGGTCGT
TTTTAGTGCTATCGACATTT

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSTQKLWGGRFTETGAKYVDDFGASISFDQLLAAEDIAGSLAHVKMLKKTGILPAADVDQIVAGLETLAERQKQGELEFS
TVNEDIHMNIESLLTEEIGPVAGKLHTARSRNDQVATDFHLYLKHQLPLILDRLHELETVLVDKASENVETVMPGYTHLQ
HAQPISYAHYLLAYYQMFKRDMERFEFNLKHTDISPLGAAALAGTTFPIDREYSAKLLGFSEVYHNSLDAVSDRDFVLEF
LSNASILMMHLSRFCEEIVSWSSYEFKYISLSDQFSTGSSIMPQKKNPDMAELIRGKSGRVYGNLMGLLTVMKGIPLAYN
KDLQEDKEGAFDTVTTVLTSLHVFTGMLATLTVNEDRMADATTNDFSNATELADYLANKGIPFREAHAIVGKLVLDGLKK
QRPLQDIPLKEYQSISPLIQEDVYHDLNAKVAVERRHSLGGTGFDQIRQELQRAQTQLSAYQSETTD

Sequences:

>Translated_467_residues
MSTQKLWGGRFTETGAKYVDDFGASISFDQLLAAEDIAGSLAHVKMLKKTGILPAADVDQIVAGLETLAERQKQGELEFS
TVNEDIHMNIESLLTEEIGPVAGKLHTARSRNDQVATDFHLYLKHQLPLILDRLHELETVLVDKASENVETVMPGYTHLQ
HAQPISYAHYLLAYYQMFKRDMERFEFNLKHTDISPLGAAALAGTTFPIDREYSAKLLGFSEVYHNSLDAVSDRDFVLEF
LSNASILMMHLSRFCEEIVSWSSYEFKYISLSDQFSTGSSIMPQKKNPDMAELIRGKSGRVYGNLMGLLTVMKGIPLAYN
KDLQEDKEGAFDTVTTVLTSLHVFTGMLATLTVNEDRMADATTNDFSNATELADYLANKGIPFREAHAIVGKLVLDGLKK
QRPLQDIPLKEYQSISPLIQEDVYHDLNAKVAVERRHSLGGTGFDQIRQELQRAQTQLSAYQSETTD
>Mature_466_residues
STQKLWGGRFTETGAKYVDDFGASISFDQLLAAEDIAGSLAHVKMLKKTGILPAADVDQIVAGLETLAERQKQGELEFST
VNEDIHMNIESLLTEEIGPVAGKLHTARSRNDQVATDFHLYLKHQLPLILDRLHELETVLVDKASENVETVMPGYTHLQH
AQPISYAHYLLAYYQMFKRDMERFEFNLKHTDISPLGAAALAGTTFPIDREYSAKLLGFSEVYHNSLDAVSDRDFVLEFL
SNASILMMHLSRFCEEIVSWSSYEFKYISLSDQFSTGSSIMPQKKNPDMAELIRGKSGRVYGNLMGLLTVMKGIPLAYNK
DLQEDKEGAFDTVTTVLTSLHVFTGMLATLTVNEDRMADATTNDFSNATELADYLANKGIPFREAHAIVGKLVLDGLKKQ
RPLQDIPLKEYQSISPLIQEDVYHDLNAKVAVERRHSLGGTGFDQIRQELQRAQTQLSAYQSETTD

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=459, Percent_Identity=43.7908496732026, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI68303542, Length=459, Percent_Identity=43.7908496732026, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI68303549, Length=459, Percent_Identity=42.0479302832244, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI68303547, Length=459, Percent_Identity=41.1764705882353, Blast_Score=351, Evalue=1e-96,
Organism=Escherichia coli, GI1790398, Length=455, Percent_Identity=47.4725274725275, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321806, Length=467, Percent_Identity=43.0406852248394, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221473854, Length=451, Percent_Identity=39.0243902439024, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI78706858, Length=451, Percent_Identity=39.0243902439024, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_LACPL (P59617)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784524.1
- ProteinModelPortal:   P59617
- SMR:   P59617
- GeneID:   1063764
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0776
- NMPDR:   fig|220668.1.peg.658
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- ProtClustDB:   PRK00855
- BioCyc:   LPLA220668:LP_0776-MONOMER
- BRENDA:   4.3.2.1
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 52271; Mature: 52140

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQKLWGGRFTETGAKYVDDFGASISFDQLLAAEDIAGSLAHVKMLKKTGILPAADVDQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IVAGLETLAERQKQGELEFSTVNEDIHMNIESLLTEEIGPVAGKLHTARSRNDQVATDFH
HHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LYLKHQLPLILDRLHELETVLVDKASENVETVMPGYTHLQHAQPISYAHYLLAYYQMFKR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHH
DMERFEFNLKHTDISPLGAAALAGTTFPIDREYSAKLLGFSEVYHNSLDAVSDRDFVLEF
HHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LSNASILMMHLSRFCEEIVSWSSYEFKYISLSDQFSTGSSIMPQKKNPDMAELIRGKSGR
HCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCC
VYGNLMGLLTVMKGIPLAYNKDLQEDKEGAFDTVTTVLTSLHVFTGMLATLTVNEDRMAD
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
ATTNDFSNATELADYLANKGIPFREAHAIVGKLVLDGLKKQRPLQDIPLKEYQSISPLIQ
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
EDVYHDLNAKVAVERRHSLGGTGFDQIRQELQRAQTQLSAYQSETTD
HHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STQKLWGGRFTETGAKYVDDFGASISFDQLLAAEDIAGSLAHVKMLKKTGILPAADVDQ
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IVAGLETLAERQKQGELEFSTVNEDIHMNIESLLTEEIGPVAGKLHTARSRNDQVATDFH
HHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LYLKHQLPLILDRLHELETVLVDKASENVETVMPGYTHLQHAQPISYAHYLLAYYQMFKR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHH
DMERFEFNLKHTDISPLGAAALAGTTFPIDREYSAKLLGFSEVYHNSLDAVSDRDFVLEF
HHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LSNASILMMHLSRFCEEIVSWSSYEFKYISLSDQFSTGSSIMPQKKNPDMAELIRGKSGR
HCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCC
VYGNLMGLLTVMKGIPLAYNKDLQEDKEGAFDTVTTVLTSLHVFTGMLATLTVNEDRMAD
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
ATTNDFSNATELADYLANKGIPFREAHAIVGKLVLDGLKKQRPLQDIPLKEYQSISPLIQ
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
EDVYHDLNAKVAVERRHSLGGTGFDQIRQELQRAQTQLSAYQSETTD
HHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566