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The map label for this gene is argG

Identifier: 28377631

GI number: 28377631

Start: 710942

End: 712177

Strand: Direct

Name: argG

Synonym: lp_0775

Alternate gene names: 28377631

Gene position: 710942-712177 (Clockwise)

Preceding gene: 28377630

Following gene: 28377632

Centisome position: 21.49

GC content: 45.63

Gene sequence:

>1236_bases
ATGGTCAAACAAAATGATAAAATTATTCTCGCTTATTCTGGTGGTTTAGATACGTCGGTCGCAATTAGTTGGTTGAAGGA
TAAGGGCTATGATGTTGTGGCCTGCGGAATTGATGTTGGTGAAGGCAAGGACATGGATGCAATCAAAGAAAAGGCCCTAA
AATTAGGGGCTGTTTCATCATATATGATTGATGCTAAGCAAGAATTTGCTGAAGAATATGCGTTAATTGCTTTACAAGGT
CATACCTTGTATGAAGGTGAATACCCGCTGGTCTCAGCACTTTCACGTCCTTTAATTGCTAAGAAGTTAGTCACATTAGC
CAAGCAAGAGCACGCTGTTGCAATTGCCCATGGTTGTACTGGGAAGGGTAATGATCAGGTTCGGTTTGAGGTTGCTATCC
ATGCTCTTGCACCGGATATCAAGATCGAAGCGCCTGTACGTGATTGGCACTGGTCACGGGAAGAAGAAATCGATTACGCT
AAGGAACACAATATTCCCGTACCAATCAATTTGGACAGCCCTTACTCGATTGACGAAAACTTATGGGGCCGGGCCAATGA
ATGTGGCATTCTAGAAGATCCATGGCAGGGCGCGCCTGCCGATGCGTTTGACCGGACGAAGGCCTTAGCTGATACGCCGG
ATACGCCAACGACTTTGGAAATCACTTTTGAAGCTGGGGTGCCAGTTGCTTTAGATGGTGAATCACTTAACCTAGCTGAT
TTAATCATCAAGTTGGATCAGATTGCTGGTGAACATGGAATTGGTCGGATCGACCATATTGAAAACCGGTTGGTCGGTAT
CAAGTCACGGGAAGTTTACGAAGCACCAGCTGCAACGGTATTGCTCAAAGCACATAAAGATTTGGAAGATTTGACGTTTG
AGCGTGAGTTAGCTCACTTCAAGCCAATCATTGAACAAAAGCTTGCGGATACGATCTACAACGGTCTTTGGTTCTCACCA
TTGATGGAAGCGATGGTTGCCTTCTTAAAGCAAACTCAGCAAGTCGTCAATGGGGTAGTTCGGGTACAACTCTTCAAGGG
TAACGTTATTACTGAGGGGCGGAAGTCACCCAACTCACTATATGATACGAACTTGGCAACCTATACATCAGCGGATTCAT
TTGACCAACAAGCGGCAGTTGGTTTCATTAAGCTATGGGGCCTACCAACGCAAGTTAATGCCCAAGTCCAAGCGAAGGCT
CAGGCCGAAGCTAAGACTGATAAGGCACACGCCTAA

Upstream 100 bases:

>100_bases
ATGATGAATATATTTAAAATATTATTGAATAAAGTTAAATTGGGTGGTACGATACTGTCATTGATAAAAGCAAAGATAAA
AATTTCGGAGGTACAAATCA

Downstream 100 bases:

>100_bases
TGAGTACCCAGAAGCTATGGGGTGGCCGGTTTACCGAAACGGGTGCTAAGTATGTTGATGACTTTGGGGCATCAATTAGC
TTTGACCAGTTGCTTGCCGC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MVKQNDKIILAYSGGLDTSVAISWLKDKGYDVVACGIDVGEGKDMDAIKEKALKLGAVSSYMIDAKQEFAEEYALIALQG
HTLYEGEYPLVSALSRPLIAKKLVTLAKQEHAVAIAHGCTGKGNDQVRFEVAIHALAPDIKIEAPVRDWHWSREEEIDYA
KEHNIPVPINLDSPYSIDENLWGRANECGILEDPWQGAPADAFDRTKALADTPDTPTTLEITFEAGVPVALDGESLNLAD
LIIKLDQIAGEHGIGRIDHIENRLVGIKSREVYEAPAATVLLKAHKDLEDLTFERELAHFKPIIEQKLADTIYNGLWFSP
LMEAMVAFLKQTQQVVNGVVRVQLFKGNVITEGRKSPNSLYDTNLATYTSADSFDQQAAVGFIKLWGLPTQVNAQVQAKA
QAEAKTDKAHA

Sequences:

>Translated_411_residues
MVKQNDKIILAYSGGLDTSVAISWLKDKGYDVVACGIDVGEGKDMDAIKEKALKLGAVSSYMIDAKQEFAEEYALIALQG
HTLYEGEYPLVSALSRPLIAKKLVTLAKQEHAVAIAHGCTGKGNDQVRFEVAIHALAPDIKIEAPVRDWHWSREEEIDYA
KEHNIPVPINLDSPYSIDENLWGRANECGILEDPWQGAPADAFDRTKALADTPDTPTTLEITFEAGVPVALDGESLNLAD
LIIKLDQIAGEHGIGRIDHIENRLVGIKSREVYEAPAATVLLKAHKDLEDLTFERELAHFKPIIEQKLADTIYNGLWFSP
LMEAMVAFLKQTQQVVNGVVRVQLFKGNVITEGRKSPNSLYDTNLATYTSADSFDQQAAVGFIKLWGLPTQVNAQVQAKA
QAEAKTDKAHA
>Mature_411_residues
MVKQNDKIILAYSGGLDTSVAISWLKDKGYDVVACGIDVGEGKDMDAIKEKALKLGAVSSYMIDAKQEFAEEYALIALQG
HTLYEGEYPLVSALSRPLIAKKLVTLAKQEHAVAIAHGCTGKGNDQVRFEVAIHALAPDIKIEAPVRDWHWSREEEIDYA
KEHNIPVPINLDSPYSIDENLWGRANECGILEDPWQGAPADAFDRTKALADTPDTPTTLEITFEAGVPVALDGESLNLAD
LIIKLDQIAGEHGIGRIDHIENRLVGIKSREVYEAPAATVLLKAHKDLEDLTFERELAHFKPIIEQKLADTIYNGLWFSP
LMEAMVAFLKQTQQVVNGVVRVQLFKGNVITEGRKSPNSLYDTNLATYTSADSFDQQAAVGFIKLWGLPTQVNAQVQAKA
QAEAKTDKAHA

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=406, Percent_Identity=41.871921182266, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI16950633, Length=406, Percent_Identity=41.871921182266, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI1789563, Length=371, Percent_Identity=27.7628032345013, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324514, Length=398, Percent_Identity=40.4522613065327, Blast_Score=285, Evalue=9e-78,
Organism=Drosophila melanogaster, GI21358151, Length=400, Percent_Identity=40.25, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_LACPL (P59603)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784523.1
- ProteinModelPortal:   P59603
- SMR:   P59603
- GeneID:   1063757
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0775
- NMPDR:   fig|220668.1.peg.657
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   LPLA220668:LP_0775-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45155; Mature: 45155

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 88-88 BINDING 118-118 BINDING 120-120 BINDING 124-124 BINDING 124-124 BINDING 125-125 BINDING 128-128 BINDING 176-176 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQNDKIILAYSGGLDTSVAISWLKDKGYDVVACGIDVGEGKDMDAIKEKALKLGAVSS
CCCCCCCEEEEECCCCCCHHEEEEHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCHHH
YMIDAKQEFAEEYALIALQGHTLYEGEYPLVSALSRPLIAKKLVTLAKQEHAVAIAHGCT
HHHHHHHHHHHCEEEEEEECCEEECCCCHHHHHHHCHHHHHHHHHHHHHCCEEEEEECCC
GKGNDQVRFEVAIHALAPDIKIEAPVRDWHWSREEEIDYAKEHNIPVPINLDSPYSIDEN
CCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCHHCCCHHHHCCCCEEEECCCCCCCCHH
LWGRANECGILEDPWQGAPADAFDRTKALADTPDTPTTLEITFEAGVPVALDGESLNLAD
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCCCCCHHH
LIIKLDQIAGEHGIGRIDHIENRLVGIKSREVYEAPAATVLLKAHKDLEDLTFERELAHF
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHCCCHHEEEEHHHCCHHHHHHHHHHHHH
KPIIEQKLADTIYNGLWFSPLMEAMVAFLKQTQQVVNGVVRVQLFKGNVITEGRKSPNSL
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCCCH
YDTNLATYTSADSFDQQAAVGFIKLWGLPTQVNAQVQAKAQAEAKTDKAHA
HCCCCEEECCCCCCCHHHHHCEEEEECCCCCCCCEEEEHHHHHHCCCCCCC
>Mature Secondary Structure
MVKQNDKIILAYSGGLDTSVAISWLKDKGYDVVACGIDVGEGKDMDAIKEKALKLGAVSS
CCCCCCCEEEEECCCCCCHHEEEEHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCHHH
YMIDAKQEFAEEYALIALQGHTLYEGEYPLVSALSRPLIAKKLVTLAKQEHAVAIAHGCT
HHHHHHHHHHHCEEEEEEECCEEECCCCHHHHHHHCHHHHHHHHHHHHHCCEEEEEECCC
GKGNDQVRFEVAIHALAPDIKIEAPVRDWHWSREEEIDYAKEHNIPVPINLDSPYSIDEN
CCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCHHCCCHHHHCCCCEEEECCCCCCCCHH
LWGRANECGILEDPWQGAPADAFDRTKALADTPDTPTTLEITFEAGVPVALDGESLNLAD
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCCCCCHHH
LIIKLDQIAGEHGIGRIDHIENRLVGIKSREVYEAPAATVLLKAHKDLEDLTFERELAHF
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHCCCHHEEEEHHHCCHHHHHHHHHHHHH
KPIIEQKLADTIYNGLWFSPLMEAMVAFLKQTQQVVNGVVRVQLFKGNVITEGRKSPNSL
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCCCH
YDTNLATYTSADSFDQQAAVGFIKLWGLPTQVNAQVQAKAQAEAKTDKAHA
HCCCCEEECCCCCCCHHHHHCEEEEECCCCCCCCEEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566