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The map label for this gene is groEL

Identifier: 28377591

GI number: 28377591

Start: 665344

End: 666969

Strand: Direct

Name: groEL

Synonym: lp_0728

Alternate gene names: 28377591

Gene position: 665344-666969 (Clockwise)

Preceding gene: 28377590

Following gene: 28377592

Centisome position: 20.11

GC content: 43.6

Gene sequence:

>1626_bases
ATGGCTAAAGAATTAAAGTTCTCTGAAGATGCACGTTCAGCGATGCTAAAAGGTGTCGATCAATTAGCTGACACAGTTAA
GTCAACGTTAGGTCCTAAGGGTCGCAACGTTGTTTTGGAACAATCATATGGTTCACCAACAATTACTAATGATGGTGTAA
CGATTGCTAAGGCGATCGAATTAGACGATCATTTCGAAAACATGGGTGCTAAGTTAGTTTCTGAAGTTGCTTCAAAGACT
AATGACATCGCTGGTGATGGGACGACTACTGCAACGGTCTTAACACAATCAATCGTTAATGAAGGTATGAAGAACGTTAC
GGCCGGTGCTAACCCTGTTGGCATTCGTCGTGGGATTGAAGAAGCTACTAAGACGGCGGTTGACTCATTACACGCTATGG
CACACGAAGTTAAGACGCAAGAAGATATTGCGCAAATCGCTTCTGTATCTTCAGCAAGTGAAGAAACTGGTAAATTGATT
GCCGAAGCCATGGAAAAAGTTGGTCATGACGGTGTTATCACGATTGAAGAATCACGTGGTGTTGATACTAGCTTAGACGT
TGTTGAAGGGATGCAATTCGACCGCGGCTACTTATCACAATACATGGTTACTGATAATGATAAGATGGAAGCGGATCTTG
ACAATCCATATATCTTAATTACTGATAAGAAGATTTCAAACATTCAAGATATCTTACCACTATTACAATCCATCGTTGAA
CAAGGCAAGCCATTGTTGATCATTGCTGATGACATTTCTGGTGAAGCTTTACCAACCTTAGTCTTGAACAAGATGCGTGG
GACGTTTAACGTTGTCGCCGTTAAGGCACCCGGTTTTGGTGATCGGCGTAAGGAACAATTACAAGATATCGCTATCTTAA
CTGGCGGGACGGTTATCACTGACGACCTTGGCCTTGAATTGAAGGACACGACCATCGATCAATTAGGTCAAGCCAACAAA
GTTACGGTTACTAAGGATAACACCACCATTGTTGAAGGCGCTGGTTCCAAGGATGCTATCTCAGAACGGGTTGAATTTAT
CCGTAACCAAATCGGTGAAACAACTTCTGACTTTGACAAAGAAAAGTTACAAGAACGTTTAGCTAAATTAGCTGGTGGGG
TTGCCGTTGTTCGTGTCGGTGCCGCTACTGAAACTGAATTGAAGGAACGTAAATACCGGATTGAAGATGCTTTGAACGCA
ACTCGGGCCGCCGTTGAAGAAGGCTTTGTTGCTGGTGGTGGTACTGCTTTGATTAACGTTATCAAAGATGTTGCTGCATT
GAAGGAAACTGGTGACGTTCAAACTGGGATCAACATTGTTAAACGTGCTTTGGAAGAACCAGTTCGCCAAATCGCTGAAA
ATGCTGGTTTAGAAGGCTCTGTTATCGTTGAAAAGATGAAGGAACAAAAGCCAGGTGTTGGTTTCAACGCCGCAACTGAT
GAATGGGTTGACATGATCAAAGCTGGTATCGTGGACCCAACTAAGGTAACGCGTTCTGCTTTACAAAATGCCGCTTCTGT
TTCAGCCCTTCTCTTAACGACTGAAGCCGTTGTCGCTGAAAAACCTGAAGAAAATGCACCAGCTGCACCAGCCGCACCAA
ACCCAGGTATGGGCGGTATGATGTAA

Upstream 100 bases:

>100_bases
TATTTGGTATTGCACGAAAAAGATATCGTTGCAATCGAAGACTAATCCATTTAAATTGCGCAACTTAAAATAAATTAAAT
TCATGAGGTGAATAGAGATA

Downstream 100 bases:

>100_bases
ACAAATCGTAACATTAAGCACTTACTCGGCTGAGTAAGTGCTTTTTTAGTGCTGTTAGGGTGTGAATCGACTAGAATAAA
GGTATTGATTTATCCATATT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MAKELKFSEDARSAMLKGVDQLADTVKSTLGPKGRNVVLEQSYGSPTITNDGVTIAKAIELDDHFENMGAKLVSEVASKT
NDIAGDGTTTATVLTQSIVNEGMKNVTAGANPVGIRRGIEEATKTAVDSLHAMAHEVKTQEDIAQIASVSSASEETGKLI
AEAMEKVGHDGVITIEESRGVDTSLDVVEGMQFDRGYLSQYMVTDNDKMEADLDNPYILITDKKISNIQDILPLLQSIVE
QGKPLLIIADDISGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLQDIAILTGGTVITDDLGLELKDTTIDQLGQANK
VTVTKDNTTIVEGAGSKDAISERVEFIRNQIGETTSDFDKEKLQERLAKLAGGVAVVRVGAATETELKERKYRIEDALNA
TRAAVEEGFVAGGGTALINVIKDVAALKETGDVQTGINIVKRALEEPVRQIAENAGLEGSVIVEKMKEQKPGVGFNAATD
EWVDMIKAGIVDPTKVTRSALQNAASVSALLLTTEAVVAEKPEENAPAAPAAPNPGMGGMM

Sequences:

>Translated_541_residues
MAKELKFSEDARSAMLKGVDQLADTVKSTLGPKGRNVVLEQSYGSPTITNDGVTIAKAIELDDHFENMGAKLVSEVASKT
NDIAGDGTTTATVLTQSIVNEGMKNVTAGANPVGIRRGIEEATKTAVDSLHAMAHEVKTQEDIAQIASVSSASEETGKLI
AEAMEKVGHDGVITIEESRGVDTSLDVVEGMQFDRGYLSQYMVTDNDKMEADLDNPYILITDKKISNIQDILPLLQSIVE
QGKPLLIIADDISGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLQDIAILTGGTVITDDLGLELKDTTIDQLGQANK
VTVTKDNTTIVEGAGSKDAISERVEFIRNQIGETTSDFDKEKLQERLAKLAGGVAVVRVGAATETELKERKYRIEDALNA
TRAAVEEGFVAGGGTALINVIKDVAALKETGDVQTGINIVKRALEEPVRQIAENAGLEGSVIVEKMKEQKPGVGFNAATD
EWVDMIKAGIVDPTKVTRSALQNAASVSALLLTTEAVVAEKPEENAPAAPAAPNPGMGGMM
>Mature_540_residues
AKELKFSEDARSAMLKGVDQLADTVKSTLGPKGRNVVLEQSYGSPTITNDGVTIAKAIELDDHFENMGAKLVSEVASKTN
DIAGDGTTTATVLTQSIVNEGMKNVTAGANPVGIRRGIEEATKTAVDSLHAMAHEVKTQEDIAQIASVSSASEETGKLIA
EAMEKVGHDGVITIEESRGVDTSLDVVEGMQFDRGYLSQYMVTDNDKMEADLDNPYILITDKKISNIQDILPLLQSIVEQ
GKPLLIIADDISGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLQDIAILTGGTVITDDLGLELKDTTIDQLGQANKV
TVTKDNTTIVEGAGSKDAISERVEFIRNQIGETTSDFDKEKLQERLAKLAGGVAVVRVGAATETELKERKYRIEDALNAT
RAAVEEGFVAGGGTALINVIKDVAALKETGDVQTGINIVKRALEEPVRQIAENAGLEGSVIVEKMKEQKPGVGFNAATDE
WVDMIKAGIVDPTKVTRSALQNAASVSALLLTTEAVVAEKPEENAPAAPAAPNPGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=526, Percent_Identity=50.1901140684411, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI31542947, Length=526, Percent_Identity=50.1901140684411, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI5453607, Length=553, Percent_Identity=23.1464737793852, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI24307939, Length=521, Percent_Identity=24.1842610364683, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI5453603, Length=154, Percent_Identity=37.012987012987, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1790586, Length=527, Percent_Identity=58.8235294117647, Blast_Score=614, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17555558, Length=532, Percent_Identity=48.4962406015038, Blast_Score=500, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI193210679, Length=213, Percent_Identity=49.7652582159624, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25144674, Length=170, Percent_Identity=27.6470588235294, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25147750, Length=183, Percent_Identity=31.6939890710383, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=52.0912547528517, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322524, Length=566, Percent_Identity=21.3780918727915, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320058, Length=178, Percent_Identity=29.2134831460674, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322049, Length=157, Percent_Identity=34.3949044585987, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=50.6641366223909, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=50.6641366223909, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45550936, Length=526, Percent_Identity=48.2889733840304, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI45550132, Length=526, Percent_Identity=48.2889733840304, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI45550935, Length=526, Percent_Identity=48.2889733840304, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI17864606, Length=540, Percent_Identity=41.2962962962963, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24584129, Length=535, Percent_Identity=35.3271028037383, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI19921262, Length=535, Percent_Identity=35.3271028037383, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI17647245, Length=529, Percent_Identity=23.4404536862004, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652903, Length=518, Percent_Identity=23.5521235521236, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24645179, Length=514, Percent_Identity=23.3463035019455, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI18858175, Length=155, Percent_Identity=33.5483870967742, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571140, Length=155, Percent_Identity=33.5483870967742, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_LACPL (Q88YM5)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784483.1
- ProteinModelPortal:   Q88YM5
- SMR:   Q88YM5
- GeneID:   1063384
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0728
- NMPDR:   fig|220668.1.peg.617
- HOGENOM:   HBG625289
- OMA:   ATDKYED
- ProtClustDB:   PRK00013
- BioCyc:   LPLA220668:LP_0728-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57438; Mature: 57307

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKFSEDARSAMLKGVDQLADTVKSTLGPKGRNVVLEQSYGSPTITNDGVTIAKAIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEC
LDDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQSIVNEGMKNVTAGANPVGIRRGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EATKTAVDSLHAMAHEVKTQEDIAQIASVSSASEETGKLIAEAMEKVGHDGVITIEESRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
VDTSLDVVEGMQFDRGYLSQYMVTDNDKMEADLDNPYILITDKKISNIQDILPLLQSIVE
CCCHHHHHHCCCCCHHHHHHHEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
QGKPLLIIADDISGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLQDIAILTGGTVIT
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHEEECCEEEE
DDLGLELKDTTIDQLGQANKVTVTKDNTTIVEGAGSKDAISERVEFIRNQIGETTSDFDK
CCCCCEECCCCHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHH
EKLQERLAKLAGGVAVVRVGAATETELKERKYRIEDALNATRAAVEEGFVAGGGTALINV
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IKDVAALKETGDVQTGINIVKRALEEPVRQIAENAGLEGSVIVEKMKEQKPGVGFNAATD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHH
EWVDMIKAGIVDPTKVTRSALQNAASVSALLLTTEAVVAEKPEENAPAAPAAPNPGMGGM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
M
C
>Mature Secondary Structure 
AKELKFSEDARSAMLKGVDQLADTVKSTLGPKGRNVVLEQSYGSPTITNDGVTIAKAIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEC
LDDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQSIVNEGMKNVTAGANPVGIRRGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EATKTAVDSLHAMAHEVKTQEDIAQIASVSSASEETGKLIAEAMEKVGHDGVITIEESRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
VDTSLDVVEGMQFDRGYLSQYMVTDNDKMEADLDNPYILITDKKISNIQDILPLLQSIVE
CCCHHHHHHCCCCCHHHHHHHEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
QGKPLLIIADDISGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLQDIAILTGGTVIT
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHEEECCEEEE
DDLGLELKDTTIDQLGQANKVTVTKDNTTIVEGAGSKDAISERVEFIRNQIGETTSDFDK
CCCCCEECCCCHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHH
EKLQERLAKLAGGVAVVRVGAATETELKERKYRIEDALNATRAAVEEGFVAGGGTALINV
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IKDVAALKETGDVQTGINIVKRALEEPVRQIAENAGLEGSVIVEKMKEQKPGVGFNAATD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHH
EWVDMIKAGIVDPTKVTRSALQNAASVSALLLTTEAVVAEKPEENAPAAPAAPNPGMGGM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
M
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566