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The map label for this gene is tmk

Identifier: 28377570

GI number: 28377570

Start: 646288

End: 646953

Strand: Direct

Name: tmk

Synonym: lp_0703

Alternate gene names: 28377570

Gene position: 646288-646953 (Clockwise)

Preceding gene: 28377569

Following gene: 28377571

Centisome position: 19.54

GC content: 48.5

Gene sequence:

>666_bases
ATGTTGACAGGAAAATTAGTTACTTTTGAAGGGCCAGACGGTGCCGGCAAAACGTCAGCTTTAAACGCGATTGTGGCCAA
ATTACAGCCACAATTAGGGGATCGCTTAGTTGTGACGCGTGAACCGGGCGGGAATCAAATCTCAGAAGCAATTCGAAAAA
TCATTTTAGACCGTCATAATACGGCGATGGACGACCGTACGGAAGCGTTACTCTATGCGGCAGCACGGCGACAACATATC
GTCCAAACCATTCAACCAGCATTGCAGAACGACCAACTTGTCTTGTGTGATCGCTACATCGATAGCTCGGTCGCTTATCA
GGGGGCCGGTCGTGGTATTGGGGAACAAGCGGTTTATGATATGAATCAGTTCGCGACCGCGGGGTTAACCGCAGATTTAA
CGTTGTATTTTGACGTAGATGCTGCCGTTGGATTAAACCGCATTCAACAACATCGCCAAAATGAAATTAATCGCTTGGAC
GTGGAAGCCTTAAGTTTTCATCACCGGGTGCAAGCAGCGTATTTGAGATTACTGGCTGATCATCCTGACCGTATCAAGCG
CGTCGATGCAAGTCAGCCATTGGACCAAGTTGTGACGCAGGCCTTAGAGATTATGGCGTCACAGTTACCGACTTATGTGG
CATCCAAGGGAGGGGAAGACCAATGA

Upstream 100 bases:

>100_bases
AAAATCGTGAAAAAGTACCCTCACTAGTGGTAACCGTATTAGACAGGTACTCAAAAATCAAGTACAATTGCTACATATAA
GAAAATAGATGGGATTGGGA

Downstream 100 bases:

>100_bases
AATTAATTATTGCAATCGTTCAAGATAAGGACAGTAACCGGTTGAGCAGCCAGTTCATTGAAGCCAATGTTCGGGCCACG
AAGCTCTCAACGACGGGGGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLTGKLVTFEGPDGAGKTSALNAIVAKLQPQLGDRLVVTREPGGNQISEAIRKIILDRHNTAMDDRTEALLYAAARRQHI
VQTIQPALQNDQLVLCDRYIDSSVAYQGAGRGIGEQAVYDMNQFATAGLTADLTLYFDVDAAVGLNRIQQHRQNEINRLD
VEALSFHHRVQAAYLRLLADHPDRIKRVDASQPLDQVVTQALEIMASQLPTYVASKGGEDQ

Sequences:

>Translated_221_residues
MLTGKLVTFEGPDGAGKTSALNAIVAKLQPQLGDRLVVTREPGGNQISEAIRKIILDRHNTAMDDRTEALLYAAARRQHI
VQTIQPALQNDQLVLCDRYIDSSVAYQGAGRGIGEQAVYDMNQFATAGLTADLTLYFDVDAAVGLNRIQQHRQNEINRLD
VEALSFHHRVQAAYLRLLADHPDRIKRVDASQPLDQVVTQALEIMASQLPTYVASKGGEDQ
>Mature_221_residues
MLTGKLVTFEGPDGAGKTSALNAIVAKLQPQLGDRLVVTREPGGNQISEAIRKIILDRHNTAMDDRTEALLYAAARRQHI
VQTIQPALQNDQLVLCDRYIDSSVAYQGAGRGIGEQAVYDMNQFATAGLTADLTLYFDVDAAVGLNRIQQHRQNEINRLD
VEALSFHHRVQAAYLRLLADHPDRIKRVDASQPLDQVVTQALEIMASQLPTYVASKGGEDQ

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=201, Percent_Identity=39.8009950248756, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_LACPL (Q88YP6)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784462.1
- ProteinModelPortal:   Q88YP6
- SMR:   Q88YP6
- GeneID:   1062598
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0703
- NMPDR:   fig|220668.1.peg.596
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK00698
- BioCyc:   LPLA220668:LP_0703-MONOMER
- BRENDA:   2.7.4.9
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 24315; Mature: 24315

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGKLVTFEGPDGAGKTSALNAIVAKLQPQLGDRLVVTREPGGNQISEAIRKIILDRHN
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
TAMDDRTEALLYAAARRQHIVQTIQPALQNDQLVLCDRYIDSSVAYQGAGRGIGEQAVYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCHHHHHCCCCCCCCHHHHHH
MNQFATAGLTADLTLYFDVDAAVGLNRIQQHRQNEINRLDVEALSFHHRVQAAYLRLLAD
HHHHHHCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HPDRIKRVDASQPLDQVVTQALEIMASQLPTYVASKGGEDQ
CHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure
MLTGKLVTFEGPDGAGKTSALNAIVAKLQPQLGDRLVVTREPGGNQISEAIRKIILDRHN
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
TAMDDRTEALLYAAARRQHIVQTIQPALQNDQLVLCDRYIDSSVAYQGAGRGIGEQAVYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCHHHHHCCCCCCCCHHHHHH
MNQFATAGLTADLTLYFDVDAAVGLNRIQQHRQNEINRLDVEALSFHHRVQAAYLRLLAD
HHHHHHCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HPDRIKRVDASQPLDQVVTQALEIMASQLPTYVASKGGEDQ
CHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566