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The map label for this gene is pts9D [H]

Identifier: 28377458

GI number: 28377458

Start: 534979

End: 535896

Strand: Direct

Name: pts9D [H]

Synonym: lp_0577

Alternate gene names: 28377458

Gene position: 534979-535896 (Clockwise)

Preceding gene: 28377457

Following gene: 28377459

Centisome position: 16.17

GC content: 40.2

Gene sequence:

>918_bases
ATGGCTGATAATAAAGTACAAATTACTAAAAGTGATCGTATTAAGGTTTGGATGCGTTCTAACTTCCTTCAAGGTTCATG
GAACTACGAACGTATGCAAAATGGTGGCTGGGCATTTACTTTAATCCCAGTACTTAAAAAGTTATATACAACTAAAGAAG
ACCGTGCCGCAGCTTTAAAGCGTCATTTGGAATTCTTTAACACTCATCCATACGTTGCCTCACCAATCATTGGTGTCACG
ATGGCGTTGGAAGAAGAACGTGCCAATGGTGCTCCAATTGATGATGTTACGATTCAAGGGGTTAAAGTTGGTATGATGGG
TCCTTTAGCTGGTGTTGGTGATCCAGTGTTCTGGTTCACGTTGAAGCCAATTATTGGTGCCTTAGCTGCTTCTCTTGCTT
TGGGCGGTAGTATCATGGGTCCAATCCTATACTTCGTTGTATGGAACGTACTTCGTTTAGGATTCATGTGGTACACACAG
GAATTCGGGTACAAAGCTGGTTCTAAGATTACTGAAGATTTATCTGGTGGTATTTTACAAGACATCACTAAAGGTGCATC
AATTCTAGGTATGTTCATCTTACCTGCTTTGATTGAACGGTGGGTTGTTGTTGATTTCAGTCCAGTTAAAGTTTCACGAA
TCAAGCAAAGTGCCGGTGCTTATATTGATTGGGATAAATTACCAAAGGGCGCTGCTGGTATCAAGGAAGCCTTAACGCAA
CAAGCTGCTGGTCGTTCGCTCGATAAGTATAAAGTTACGACTTTACAAGATAACTTAAATCAATTAATTCCAGGATTAGC
TGCCTTATTAATCACCTTCTTGTCAATGTGGTTACTAAAGAAGAAAGTTTCACCAATTATTATCATCTTCGGCCTCTTCG
CCTTTGGTGTTGTTATGCATGTTCTTGGTGTAATGTAA

Upstream 100 bases:

>100_bases
ACCTTTCTAAGATGGGCGGTTCTGGTAATGGCGGTGGCTCTAACACTGGTGACCCTGTCGGCGATATTATCGATAAATAC
TAACGAAGGAGGGAAAGAAA

Downstream 100 bases:

>100_bases
AAAGCTATTGTTAACATAATATTGGGGACTTCAATCTGAACTTTTAATTTATAGTGAAGCTCGTTGATAGATTTAGATGG
ACTCATTCGTGAGGCCATCT

Product: mannose PTS, EIID

Products: protein histidine; sugar phosphate

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MADNKVQITKSDRIKVWMRSNFLQGSWNYERMQNGGWAFTLIPVLKKLYTTKEDRAAALKRHLEFFNTHPYVASPIIGVT
MALEEERANGAPIDDVTIQGVKVGMMGPLAGVGDPVFWFTLKPIIGALAASLALGGSIMGPILYFVVWNVLRLGFMWYTQ
EFGYKAGSKITEDLSGGILQDITKGASILGMFILPALIERWVVVDFSPVKVSRIKQSAGAYIDWDKLPKGAAGIKEALTQ
QAAGRSLDKYKVTTLQDNLNQLIPGLAALLITFLSMWLLKKKVSPIIIIFGLFAFGVVMHVLGVM

Sequences:

>Translated_305_residues
MADNKVQITKSDRIKVWMRSNFLQGSWNYERMQNGGWAFTLIPVLKKLYTTKEDRAAALKRHLEFFNTHPYVASPIIGVT
MALEEERANGAPIDDVTIQGVKVGMMGPLAGVGDPVFWFTLKPIIGALAASLALGGSIMGPILYFVVWNVLRLGFMWYTQ
EFGYKAGSKITEDLSGGILQDITKGASILGMFILPALIERWVVVDFSPVKVSRIKQSAGAYIDWDKLPKGAAGIKEALTQ
QAAGRSLDKYKVTTLQDNLNQLIPGLAALLITFLSMWLLKKKVSPIIIIFGLFAFGVVMHVLGVM
>Mature_304_residues
ADNKVQITKSDRIKVWMRSNFLQGSWNYERMQNGGWAFTLIPVLKKLYTTKEDRAAALKRHLEFFNTHPYVASPIIGVTM
ALEEERANGAPIDDVTIQGVKVGMMGPLAGVGDPVFWFTLKPIIGALAASLALGGSIMGPILYFVVWNVLRLGFMWYTQE
FGYKAGSKITEDLSGGILQDITKGASILGMFILPALIERWVVVDFSPVKVSRIKQSAGAYIDWDKLPKGAAGIKEALTQQ
AAGRSLDKYKVTTLQDNLNQLIPGLAALLITFLSMWLLKKKVSPIIIIFGLFAFGVVMHVLGVM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=300, Percent_Identity=47, Blast_Score=290, Evalue=5e-80,
Organism=Escherichia coli, GI1789529, Length=306, Percent_Identity=32.0261437908497, Blast_Score=154, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 33626; Mature: 33495

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNKVQITKSDRIKVWMRSNFLQGSWNYERMQNGGWAFTLIPVLKKLYTTKEDRAAALK
CCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHH
RHLEFFNTHPYVASPIIGVTMALEEERANGAPIDDVTIQGVKVGMMGPLAGVGDPVFWFT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHH
LKPIIGALAASLALGGSIMGPILYFVVWNVLRLGFMWYTQEFGYKAGSKITEDLSGGILQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHH
DITKGASILGMFILPALIERWVVVDFSPVKVSRIKQSAGAYIDWDKLPKGAAGIKEALTQ
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCEEEHHHCCCCHHHHHHHHHH
QAAGRSLDKYKVTTLQDNLNQLIPGLAALLITFLSMWLLKKKVSPIIIIFGLFAFGVVMH
HHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VLGVM
HHHCC
>Mature Secondary Structure 
ADNKVQITKSDRIKVWMRSNFLQGSWNYERMQNGGWAFTLIPVLKKLYTTKEDRAAALK
CCCEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHH
RHLEFFNTHPYVASPIIGVTMALEEERANGAPIDDVTIQGVKVGMMGPLAGVGDPVFWFT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHH
LKPIIGALAASLALGGSIMGPILYFVVWNVLRLGFMWYTQEFGYKAGSKITEDLSGGILQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHH
DITKGASILGMFILPALIERWVVVDFSPVKVSRIKQSAGAYIDWDKLPKGAAGIKEALTQ
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCEEEHHHCCCCHHHHHHHHHH
QAAGRSLDKYKVTTLQDNLNQLIPGLAALLITFLSMWLLKKKVSPIIIIFGLFAFGVVMH
HHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VLGVM
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]