Click here to switch to the map view.

The map label for this gene is pts9AB [H]

Identifier: 28377456

GI number: 28377456

Start: 533143

End: 534117

Strand: Direct

Name: pts9AB [H]

Synonym: lp_0575

Alternate gene names: 28377456

Gene position: 533143-534117 (Clockwise)

Preceding gene: 28377455

Following gene: 28377457

Centisome position: 16.12

GC content: 43.08

Gene sequence:

>975_bases
ATGGTAAGCATTATCATTGCTAGTCACGGCGAATTCGCTAAAGGCATCTATCAATCTGGATCAATGATTTTCGGCGAACA
AGAAAACGTTCAAGCCGTTACGTTGATGCCTAGCGAAGGCCCTGACGACATCAAGGCTAAGTTGGAAAAAGCCATTGCTT
CATTCGACGATCAAGAGCAAGTCTTATTCTTAGTCGACCTTTGGGGCGGTACACCATTCAATCAAGTTAACGGCTTGTTT
GAAGCACACAAGGACAAATGGGCAATCGTTGCCGGTTTGAACTTGCCAATGTTAATTGAAGCGTATGCTTCACGACTTTC
AATGGATTCGGCACACGAAATTGCCACGCATATCATTGAAACTGCTAAGGATGGCGTAAAGGTTCGTCCTGAAGCATTAG
CACCTAAGGAAGAAAAGGCTGCTGCCGCTGCAACGACCAACAATAACGCTGGCCGGCCAGGGAAGTTGGAATATGGGTTA
GCACGGATTGACTCGCGACTCTTGCATGGACAAGTTGCTACTGCTTGGAGTAAGACAGTTAACCCAACTCGGATCATTGT
TGTTTCCGATAGTGTTGCTAAGGATGATTTACGTTCAAACATGATTAAGCAAGCTGCTCCAGCAGGCGTACATGCTCATG
TTATTCCAATTGATCAAATGATTAAGATTGCTAAAGACGACAAGCACTTTGGTGGTCAAAAGGCGTTGGTCTTATTCGAA
ACGCCACAAGATGCATTACGTGCAATCGAGGGTGGCGTGCCAATTAAGACCTTGAACATTGGTTCAATGGCGCACTCAGT
TGGTAAAGTTCAACCAAACAAAGTTTTGGCTTTTGATCAAGATGATATTGATACTTACAAGAAGTTGGAACAAGACGGCG
TCGAATTTGATGTTCGTAAAGTTCCTTCAGATAGTAAGGATAATATGGATAATATTCTTAAAAAGGCTCAAAACATGTTA
AATGAACAAAAGTAG

Upstream 100 bases:

>100_bases
AGATTGCGCTTACAGTATCAAAAACATAAATAGTTACAGGCAATTTAGATATTATTTATTCGTTTTATATGACTGTAACG
AAAAAATAGGAGGTACAAAG

Downstream 100 bases:

>100_bases
TATAGATAACTATCAGAAGAAAATGGAGGATTAAATCATGAATTTGAATGCAATTCAAATGATTTTAGTCGTACTCGTTG
CATTTTTAGCTGGTATGGAA

Product: mannose PTS, EIIAB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MVSIIIASHGEFAKGIYQSGSMIFGEQENVQAVTLMPSEGPDDIKAKLEKAIASFDDQEQVLFLVDLWGGTPFNQVNGLF
EAHKDKWAIVAGLNLPMLIEAYASRLSMDSAHEIATHIIETAKDGVKVRPEALAPKEEKAAAAATTNNNAGRPGKLEYGL
ARIDSRLLHGQVATAWSKTVNPTRIIVVSDSVAKDDLRSNMIKQAAPAGVHAHVIPIDQMIKIAKDDKHFGGQKALVLFE
TPQDALRAIEGGVPIKTLNIGSMAHSVGKVQPNKVLAFDQDDIDTYKKLEQDGVEFDVRKVPSDSKDNMDNILKKAQNML
NEQK

Sequences:

>Translated_324_residues
MVSIIIASHGEFAKGIYQSGSMIFGEQENVQAVTLMPSEGPDDIKAKLEKAIASFDDQEQVLFLVDLWGGTPFNQVNGLF
EAHKDKWAIVAGLNLPMLIEAYASRLSMDSAHEIATHIIETAKDGVKVRPEALAPKEEKAAAAATTNNNAGRPGKLEYGL
ARIDSRLLHGQVATAWSKTVNPTRIIVVSDSVAKDDLRSNMIKQAAPAGVHAHVIPIDQMIKIAKDDKHFGGQKALVLFE
TPQDALRAIEGGVPIKTLNIGSMAHSVGKVQPNKVLAFDQDDIDTYKKLEQDGVEFDVRKVPSDSKDNMDNILKKAQNML
NEQK
>Mature_324_residues
MVSIIIASHGEFAKGIYQSGSMIFGEQENVQAVTLMPSEGPDDIKAKLEKAIASFDDQEQVLFLVDLWGGTPFNQVNGLF
EAHKDKWAIVAGLNLPMLIEAYASRLSMDSAHEIATHIIETAKDGVKVRPEALAPKEEKAAAAATTNNNAGRPGKLEYGL
ARIDSRLLHGQVATAWSKTVNPTRIIVVSDSVAKDDLRSNMIKQAAPAGVHAHVIPIDQMIKIAKDDKHFGGQKALVLFE
TPQDALRAIEGGVPIKTLNIGSMAHSVGKVQPNKVLAFDQDDIDTYKKLEQDGVEFDVRKVPSDSKDNMDNILKKAQNML
NEQK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=320, Percent_Identity=39.6875, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI87082217, Length=156, Percent_Identity=32.0512820512821, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=33.5526315789474, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35284; Mature: 35284

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIIIASHGEFAKGIYQSGSMIFGEQENVQAVTLMPSEGPDDIKAKLEKAIASFDDQEQ
CEEEEEECCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
VLFLVDLWGGTPFNQVNGLFEAHKDKWAIVAGLNLPMLIEAYASRLSMDSAHEIATHIIE
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHH
TAKDGVKVRPEALAPKEEKAAAAATTNNNAGRPGKLEYGLARIDSRLLHGQVATAWSKTV
HHCCCCEECHHHCCCCHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NPTRIIVVSDSVAKDDLRSNMIKQAAPAGVHAHVIPIDQMIKIAKDDKHFGGQKALVLFE
CCEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCCEEEEEEE
TPQDALRAIEGGVPIKTLNIGSMAHSVGKVQPNKVLAFDQDDIDTYKKLEQDGVEFDVRK
CCHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEE
VPSDSKDNMDNILKKAQNMLNEQK
CCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSIIIASHGEFAKGIYQSGSMIFGEQENVQAVTLMPSEGPDDIKAKLEKAIASFDDQEQ
CEEEEEECCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
VLFLVDLWGGTPFNQVNGLFEAHKDKWAIVAGLNLPMLIEAYASRLSMDSAHEIATHIIE
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHH
TAKDGVKVRPEALAPKEEKAAAAATTNNNAGRPGKLEYGLARIDSRLLHGQVATAWSKTV
HHCCCCEECHHHCCCCHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NPTRIIVVSDSVAKDDLRSNMIKQAAPAGVHAHVIPIDQMIKIAKDDKHFGGQKALVLFE
CCEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCCEEEEEEE
TPQDALRAIEGGVPIKTLNIGSMAHSVGKVQPNKVLAFDQDDIDTYKKLEQDGVEFDVRK
CCHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEE
VPSDSKDNMDNILKKAQNMLNEQK
CCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA