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The map label for this gene is pgk [H]

Identifier: 28199716

GI number: 28199716

Start: 2165051

End: 2166226

Strand: Reverse

Name: pgk [H]

Synonym: PD1847

Alternate gene names: 28199716

Gene position: 2166226-2165051 (Counterclockwise)

Preceding gene: 77747714

Following gene: 28199715

Centisome position: 85.97

GC content: 55.44

Gene sequence:

>1176_bases
ATGTCCATCGTCCGCATGACTGATCTCGCTTTATCCGGTAAACGCGTACTGATTCGTCAGGACTTGAATGTACCGATTGA
TCAGGGTCGTATTACTTCCGAGCAGCGCATTATTGCTTCGCTTCCGACGATCCGTGTAGCGCTGGAGCGTGGTGCGGCGG
TCATGGTGACTTCGCATCTGGGCCGTCCGAAGGAGGGCGTGTGGAGTGAGGAGGATTCATTGGCACCCGTGGCGAAGCGT
TTGTCTCAATTGTTGGGGATTGAGGTTCCGTTGCGTCGCGATTGGGTGGATGGCGTGCAGGTTGCGCCAGGGCAGTTGGT
GTTATTGGAAAACTGCCGAATGAATGTCGGCGAAGCTGAGAATGACGAGGCGTTGGCAAGGAAGTATGCGGCGTTATGTG
ATGTGTTCGTGATGGATGCGTTTGGCACTGCGCACCGTGCCCAGGCATCAACGCATGGGGTGATCTGTTGTGCTGCGATT
GCTGCCGGCGGTCCGTTGTTGATGGCTGAGTTGGATGCTTTGAGCCGGGGATTGAAGCATCCGGTGAAGCCGCTGCTGGC
GATTGTGGGGGGCAGTAAGGTATCGACGAAATTGGAGTTGCTTTCCAATTTGGTGAATAACGTTGAGCAATTGATTACGG
GGGGAGGCATTGCCAATACGTTCCTTGCCGCAGCCGGTTACCCCATCGGTAAGTCACTGTATGAGGCGGATTTGATCGAG
ACTGCTCGGGAGATTATTACTGTGGCGAAAGCGCGTGGCGCTGAGATTCCGTTACCCACGGATGTGGTTGTTGCCAAGCA
GTTTTTGCCTGGAGTCACGGCAACGGTGAAGACGGTGGATGCAATCGTTGCCGATGATCTTATTTTGGACATTGGTCCGC
AGACGGCACGGCACTATGCTGCCTTGATTGAAACGGCGGCTACGGTGGTCTGGAATGGTCCAGTGGGTGTGTTTGAGTTC
GATGCCTTCAGCAAGGGGACCGAGGTGTTAGCGCGGGCCGTTGCTGCCTCGTCTGCGTTTTCGATTGCCGGTGGCGGCGA
TACCTTGGCTGCTATTGATAAGTATGGTGTTGCGGACCAGATCAGTTACATCTCCACAGGCGGCGGTGCTTTTCTGGAGT
TTTTGGAAGGTAAGACGTTGCCGGCGGTGGCTGCGTTGCAGGCGCGCAGCGGATAA

Upstream 100 bases:

>100_bases
TGCTGTCATCGGAGCTAGGTTGGTGGGATGGACGCTTGGTGTTTACCTACTGCCAGATGAACCAGGACAATGGCGTTTTG
TTTCTATAAAGGAAATCGAG

Downstream 100 bases:

>100_bases
GCCGTGTGTTGAGTCCAATGGTGTGTTGATCCATTCAAGTGGATATCGTTACGGGGTTACTGCCTCGTTTTATCGTGTTT
GGTGATGGGGTGCCGGAAGC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSIVRMTDLALSGKRVLIRQDLNVPIDQGRITSEQRIIASLPTIRVALERGAAVMVTSHLGRPKEGVWSEEDSLAPVAKR
LSQLLGIEVPLRRDWVDGVQVAPGQLVLLENCRMNVGEAENDEALARKYAALCDVFVMDAFGTAHRAQASTHGVICCAAI
AAGGPLLMAELDALSRGLKHPVKPLLAIVGGSKVSTKLELLSNLVNNVEQLITGGGIANTFLAAAGYPIGKSLYEADLIE
TAREIITVAKARGAEIPLPTDVVVAKQFLPGVTATVKTVDAIVADDLILDIGPQTARHYAALIETAATVVWNGPVGVFEF
DAFSKGTEVLARAVAASSAFSIAGGGDTLAAIDKYGVADQISYISTGGGAFLEFLEGKTLPAVAALQARSG

Sequences:

>Translated_391_residues
MSIVRMTDLALSGKRVLIRQDLNVPIDQGRITSEQRIIASLPTIRVALERGAAVMVTSHLGRPKEGVWSEEDSLAPVAKR
LSQLLGIEVPLRRDWVDGVQVAPGQLVLLENCRMNVGEAENDEALARKYAALCDVFVMDAFGTAHRAQASTHGVICCAAI
AAGGPLLMAELDALSRGLKHPVKPLLAIVGGSKVSTKLELLSNLVNNVEQLITGGGIANTFLAAAGYPIGKSLYEADLIE
TAREIITVAKARGAEIPLPTDVVVAKQFLPGVTATVKTVDAIVADDLILDIGPQTARHYAALIETAATVVWNGPVGVFEF
DAFSKGTEVLARAVAASSAFSIAGGGDTLAAIDKYGVADQISYISTGGGAFLEFLEGKTLPAVAALQARSG
>Mature_390_residues
SIVRMTDLALSGKRVLIRQDLNVPIDQGRITSEQRIIASLPTIRVALERGAAVMVTSHLGRPKEGVWSEEDSLAPVAKRL
SQLLGIEVPLRRDWVDGVQVAPGQLVLLENCRMNVGEAENDEALARKYAALCDVFVMDAFGTAHRAQASTHGVICCAAIA
AGGPLLMAELDALSRGLKHPVKPLLAIVGGSKVSTKLELLSNLVNNVEQLITGGGIANTFLAAAGYPIGKSLYEADLIET
AREIITVAKARGAEIPLPTDVVVAKQFLPGVTATVKTVDAIVADDLILDIGPQTARHYAALIETAATVVWNGPVGVFEFD
AFSKGTEVLARAVAASSAFSIAGGGDTLAAIDKYGVADQISYISTGGGAFLEFLEGKTLPAVAALQARSG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=40.5867970660147, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=39.8533007334963, Blast_Score=271, Evalue=6e-73,
Organism=Escherichia coli, GI1789294, Length=390, Percent_Identity=61.2820512820513, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17508823, Length=406, Percent_Identity=44.8275862068966, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=42.8915662650602, Blast_Score=288, Evalue=9e-79,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=41.8886198547215, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24581203, Length=409, Percent_Identity=40.0977995110024, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40954; Mature: 40823

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVRMTDLALSGKRVLIRQDLNVPIDQGRITSEQRIIASLPTIRVALERGAAVMVTSHL
CCEEEEEHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCEEEEEHHC
GRPKEGVWSEEDSLAPVAKRLSQLLGIEVPLRRDWVDGVQVAPGQLVLLENCRMNVGEAE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCEECCCCEEEEECCCCCCCCCC
NDEALARKYAALCDVFVMDAFGTAHRAQASTHGVICCAAIAAGGPLLMAELDALSRGLKH
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHEEHHHHCCCCHHHHHHHHHHHHHCC
PVKPLLAIVGGSKVSTKLELLSNLVNNVEQLITGGGIANTFLAAAGYPIGKSLYEADLIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHH
TAREIITVAKARGAEIPLPTDVVVAKQFLPGVTATVKTVDAIVADDLILDIGPQTARHYA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHEECCCHHHHHHH
ALIETAATVVWNGPVGVFEFDAFSKGTEVLARAVAASSAFSIAGGGDTLAAIDKYGVADQ
HHHHHHHHEEECCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCHHH
ISYISTGGGAFLEFLEGKTLPAVAALQARSG
HHHEECCCHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SIVRMTDLALSGKRVLIRQDLNVPIDQGRITSEQRIIASLPTIRVALERGAAVMVTSHL
CEEEEEHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCEEEEEHHC
GRPKEGVWSEEDSLAPVAKRLSQLLGIEVPLRRDWVDGVQVAPGQLVLLENCRMNVGEAE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCEECCCCEEEEECCCCCCCCCC
NDEALARKYAALCDVFVMDAFGTAHRAQASTHGVICCAAIAAGGPLLMAELDALSRGLKH
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHEEHHHHCCCCHHHHHHHHHHHHHCC
PVKPLLAIVGGSKVSTKLELLSNLVNNVEQLITGGGIANTFLAAAGYPIGKSLYEADLIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHH
TAREIITVAKARGAEIPLPTDVVVAKQFLPGVTATVKTVDAIVADDLILDIGPQTARHYA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHEECCCHHHHHHH
ALIETAATVVWNGPVGVFEFDAFSKGTEVLARAVAASSAFSIAGGGDTLAAIDKYGVADQ
HHHHHHHHEEECCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCHHH
ISYISTGGGAFLEFLEGKTLPAVAALQARSG
HHHEECCCHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]