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The map label for this gene is rlmN

Identifier: 28199501

GI number: 28199501

Start: 1879937

End: 1881157

Strand: Reverse

Name: rlmN

Synonym: PD1624

Alternate gene names: 28199501

Gene position: 1881157-1879937 (Counterclockwise)

Preceding gene: 28199502

Following gene: 28199500

Centisome position: 74.65

GC content: 49.88

Gene sequence:

>1221_bases
ATGAATGGGTTTGCGGTGATTCCCAGTGTGACAACAACGACAACTAGTGGTGAGCCCATCGCTAGCGATGTTGCTCGTAA
ACAGAATCTGCTGGAACTTGACCGTGAGGGGTTGGAGCGCTTTTTCGAAGATGTTCTTGGTGAGAAACGCTATCGTGCCC
ATCAGGTCATGAAATGGATCCACCACCGTTATGTCGCTGATTTCGAGCAAATGACCGATGTTGGCAAAGCGTTGCGTACT
AGGTTGCAGGCTTGCGCTGAGGTCAGGGTCCCCCGTGTTGTCTTCGACAAGCACTCAGCCGATGGCACACACAAGTGGTT
GCTGGCGATGGGCACTGACAGAAAAAATGCGATTGAGACTGTTTATATCCCGGACAAAGGGCGTGGTACGTTGTGTGTGT
CTTCGCAAATAGGGTGTGGATTGAACTGTACGTTTTGTTCGACAGCCACCCAAGGTTTTAACCGCAATCTGACCACGGCC
GAGATTATCGGCCAGGTTTGGGTTGCTGCCCGTCACCTTGGTAATGTGCCGCACCAGCAGCGTCGTCTTACGAATGTGGT
AATGATGGGTATGGGTGAGCCCTTAATGAATTTCGATAACGTTGTGCGTGCGATGAGCGTGATGCGTGATGATTTGGGCT
ACGGGTTGTCCAACAAGCGTGTGACTTTATCGACTTCCGGTTTAGTTCCGATGATTGATCGCCTCTCGACTGAGAGTGAT
GTGTCTTTGGCTGTATCACTGCATGCGCCGAATGACAAACTGCGCGAACAACTTGTTCCGTTGAATAAGAAATATCCAAT
TGTTGAGCTGATGGCATCCTGCGAGCGTTATTTGTCTGTTAATCGTAAACGTGATTCTGTCACTTTTGAATACACTTTGA
TGAAGGGTGTTAATGACAAGCAAGAACATGCTCATGAGTTGGCCAAACTAATGCGGCAATTTGACTGTGCGATGCAGGTG
AAGGGTGCAGCGAAGGTTAACCTGATTCCATTCAACCCGTTTCCGGGCACCTGTTATGAACGTTCTACGGAAGTTGATAT
CCGTGCTTTCCAGAAGATCCTGTTGGACGCACAAATTTTGGCCATGGTGCGTCGTACTCGTGGAGACGACATTGATGCAG
CGTGCGGGCAATTGAAAGGGCAAGTCGTGGATCGTACTCGCCGTCAAGCTGAGTTCCGCCGCACTATTGAAGATCGGGTT
GGTCGAGATGTTGCAGCGTGA

Upstream 100 bases:

>100_bases
GCCAATGCTGCACATGGTTCGGATTCGGTCGAGAATGCTGCGATTGAAGTGGCTTACTTTTTCGCTGCTACCGAAATTAT
TTTGCGATGAGATTGTAGGC

Downstream 100 bases:

>100_bases
TATATCCCTGGTTTTAGCGTTTTCTTCTTTACTGATTTTATCTGGCTGCTTAGTCATGTCTGATAAGAGGGAGACACGTA
TTAAGTCAGTTCAGAATCTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNGFAVIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYVADFEQMTDVGKALRT
RLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTA
EIIGQVWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESD
VSLAVSLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAHELAKLMRQFDCAMQV
KGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRV
GRDVAA

Sequences:

>Translated_406_residues
MNGFAVIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYVADFEQMTDVGKALRT
RLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTA
EIIGQVWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESD
VSLAVSLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAHELAKLMRQFDCAMQV
KGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRV
GRDVAA
>Mature_406_residues
MNGFAVIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYVADFEQMTDVGKALRT
RLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTA
EIIGQVWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESD
VSLAVSLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAHELAKLMRQFDCAMQV
KGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRV
GRDVAA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=364, Percent_Identity=54.9450549450549, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_XYLF2 (B2I7V5)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830394.1
- ProteinModelPortal:   B2I7V5
- GeneID:   6202886
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1716
- HOGENOM:   HBG335345
- ProtClustDB:   CLSK446020
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 45706; Mature: 45706

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFAVIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWI
CCCCEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
HHRYVADFEQMTDVGKALRTRLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCEEE
VYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQVWVAARHLGNVPHQQ
EEECCCCCCEEEEECCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
RRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESD
HHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCC
VSLAVSLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDK
CEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
QEHAHELAKLMRQFDCAMQVKGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQIL
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNGFAVIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWI
CCCCEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
HHRYVADFEQMTDVGKALRTRLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCEEE
VYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQVWVAARHLGNVPHQQ
EEECCCCCCEEEEECCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
RRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESD
HHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCC
VSLAVSLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDK
CEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
QEHAHELAKLMRQFDCAMQVKGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQIL
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA