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The map label for this gene is recA [H]

Identifier: 62185564

GI number: 62185564

Start: 1127088

End: 1128137

Strand: Direct

Name: recA [H]

Synonym: CAB965

Alternate gene names: 62185564

Gene position: 1127088-1128137 (Clockwise)

Preceding gene: 62185563

Following gene: 62185571

Centisome position: 98.49

GC content: 41.14

Gene sequence:

>1050_bases
ATGAATGTACCTGATCGTAAAAAAGCACTAGAAGCAGCAATTGCCTATATTGAAAAACAATTTGGCTCTGGATCTATCAT
GAGTCTAGGGAAACACTCCGCATCTCATGAGATCTCTACTATAAAAACAGGGGCCTTATCTTTAGATTTAGCATTAGGCA
TCGGAGGTGTTCCAAAAGGCAGAATCGTTGAGATCTTTGGCCCTGAATCTTCAGGGAAAACGACGCTCGCCACTCACATC
GTGGCCAATGCGCAAAAAATGGGGGGTGTGGCTGCTTATATCGATGCAGAACACGCTTTAGATCCCAGTTACGCTTCCCT
GATAGGAGCGAATATCCATGATCTTATGATCTCCCAACCCGATTGTGGTGAGGATGCCTTAAGCATAGCGGAATTACTCG
CAAGATCAGGAGCTGTTGATGTTATCGTCATAGACTCTGTAGCCGCTTTGGTTCCTAAAAGTGAACTCGAAGGCGATATT
GGTGATGTACATGTAGGCTTACAAGCCCGTATGATGTCTCAAGCATTACGTAAGCTGACAGCAACATTAGCACGTAGTCA
AACCTGTGCGATATTCATTAACCAAATCCGAGAGAAAATAGGTGTAAGTTTTGGTAATCCCGAAACAACAACAGGCGGAC
GCGCTTTAAAATTCTACTCATCAATACGTATAGATATTCGTCGTATAGGAGCAATCAAAGGTAATGAAAGCTTCGATCTT
GGGAACCGGATAAAAGTCAAAGTGGCTAAAAATAAACTCGCCCCTCCATTCAAAACAGCAGAATTTGATATTCTCTTTAA
CGAAGGTATTTCTTCCGCAGGATGCATTTTAGATCTGGCTGTAGAACATAATATCGTAGAGAAAAAGGGATCTTGGTTTA
ACTATCAAGATCGTAAATTAGGACAAGGAAGAGAAGCTGTTCGTGAAGAACTCAAGAAAAATAAGAAATTACTCGAAGAA
TTAGAAAAACGTATTCTTGAAGTGACTGCTTCACCAAAAACTATTGTTGAAGAGAAGAAAGAAGAGCTTGCTATGCAACC
TGTAGCTTAA

Upstream 100 bases:

>100_bases
CTTAGCTGAGTTACGAGAAGAAGCTTACCCATTCTTCTCTCAAAGTCCAAAATGCAGGACTATTGCATAGAAAGTGTGGA
AAAAAGGAGCACCTATATAA

Downstream 100 bases:

>100_bases
ATTGTTTAATCTCCTTCTCCTGATTGAATCAAGAGAAGGAGAAACAAGCTCATTAATGACCGCAATTACAACGACATGAA
GAATGTTGGGAATCCGAAGT

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNVPDRKKALEAAIAYIEKQFGSGSIMSLGKHSASHEISTIKTGALSLDLALGIGGVPKGRIVEIFGPESSGKTTLATHI
VANAQKMGGVAAYIDAEHALDPSYASLIGANIHDLMISQPDCGEDALSIAELLARSGAVDVIVIDSVAALVPKSELEGDI
GDVHVGLQARMMSQALRKLTATLARSQTCAIFINQIREKIGVSFGNPETTTGGRALKFYSSIRIDIRRIGAIKGNESFDL
GNRIKVKVAKNKLAPPFKTAEFDILFNEGISSAGCILDLAVEHNIVEKKGSWFNYQDRKLGQGREAVREELKKNKKLLEE
LEKRILEVTASPKTIVEEKKEELAMQPVA

Sequences:

>Translated_349_residues
MNVPDRKKALEAAIAYIEKQFGSGSIMSLGKHSASHEISTIKTGALSLDLALGIGGVPKGRIVEIFGPESSGKTTLATHI
VANAQKMGGVAAYIDAEHALDPSYASLIGANIHDLMISQPDCGEDALSIAELLARSGAVDVIVIDSVAALVPKSELEGDI
GDVHVGLQARMMSQALRKLTATLARSQTCAIFINQIREKIGVSFGNPETTTGGRALKFYSSIRIDIRRIGAIKGNESFDL
GNRIKVKVAKNKLAPPFKTAEFDILFNEGISSAGCILDLAVEHNIVEKKGSWFNYQDRKLGQGREAVREELKKNKKLLEE
LEKRILEVTASPKTIVEEKKEELAMQPVA
>Mature_349_residues
MNVPDRKKALEAAIAYIEKQFGSGSIMSLGKHSASHEISTIKTGALSLDLALGIGGVPKGRIVEIFGPESSGKTTLATHI
VANAQKMGGVAAYIDAEHALDPSYASLIGANIHDLMISQPDCGEDALSIAELLARSGAVDVIVIDSVAALVPKSELEGDI
GDVHVGLQARMMSQALRKLTATLARSQTCAIFINQIREKIGVSFGNPETTTGGRALKFYSSIRIDIRRIGAIKGNESFDL
GNRIKVKVAKNKLAPPFKTAEFDILFNEGISSAGCILDLAVEHNIVEKKGSWFNYQDRKLGQGREAVREELKKNKKLLEE
LEKRILEVTASPKTIVEEKKEELAMQPVA

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=327, Percent_Identity=59.3272171253823, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 37601; Mature: 37601

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPDRKKALEAAIAYIEKQFGSGSIMSLGKHSASHEISTIKTGALSLDLALGIGGVPKG
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHEECCCEEEEEEECCCCCCCC
RIVEIFGPESSGKTTLATHIVANAQKMGGVAAYIDAEHALDPSYASLIGANIHDLMISQP
CEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECHHHCCCCHHHHHHCCCHHHHEECCC
DCGEDALSIAELLARSGAVDVIVIDSVAALVPKSELEGDIGDVHVGLQARMMSQALRKLT
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHCCCCCCEEEHHHHHHHHHHHHHHHH
ATLARSQTCAIFINQIREKIGVSFGNPETTTGGRALKFYSSIRIDIRRIGAIKGNESFDL
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCC
GNRIKVKVAKNKLAPPFKTAEFDILFNEGISSAGCILDLAVEHNIVEKKGSWFNYQDRKL
CCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCHHHC
GQGREAVREELKKNKKLLEELEKRILEVTASPKTIVEEKKEELAMQPVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNVPDRKKALEAAIAYIEKQFGSGSIMSLGKHSASHEISTIKTGALSLDLALGIGGVPKG
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHEECCCEEEEEEECCCCCCCC
RIVEIFGPESSGKTTLATHIVANAQKMGGVAAYIDAEHALDPSYASLIGANIHDLMISQP
CEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECHHHCCCCHHHHHHCCCHHHHEECCC
DCGEDALSIAELLARSGAVDVIVIDSVAALVPKSELEGDIGDVHVGLQARMMSQALRKLT
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHCCCCCCEEEHHHHHHHHHHHHHHHH
ATLARSQTCAIFINQIREKIGVSFGNPETTTGGRALKFYSSIRIDIRRIGAIKGNESFDL
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCC
GNRIKVKVAKNKLAPPFKTAEFDILFNEGISSAGCILDLAVEHNIVEKKGSWFNYQDRKL
CCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCHHHC
GQGREAVREELKKNKKLLEELEKRILEVTASPKTIVEEKKEELAMQPVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]