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The map label for this gene is pdhD [H]

Identifier: 62185502

GI number: 62185502

Start: 1056005

End: 1057393

Strand: Direct

Name: pdhD [H]

Synonym: CAB902

Alternate gene names: 62185502

Gene position: 1056005-1057393 (Clockwise)

Preceding gene: 62185500

Following gene: 62185503

Centisome position: 92.28

GC content: 43.41

Gene sequence:

>1389_bases
ATGAGTACAGATTTTGACTGTGTTGTTATTGGTGCTGGACCTGGTGGTTACGTTGCAGCAATTACCGCTGCGCAACAAGG
ACTGAAGACAGCACTTATTGAAGAACAACAAGCCGGCGGAACATGTTTAAATCGCGGCTGTATTCCATCTAAAGCCCTAT
TAGTAGGTGCAGGAATTGTTTCCCAAATTAAACATGCAAACCAATTTGGCATACATATTGATGGCTATTCCATAGACTAT
CCCGCCATGGTTCAAAGAAAAAATACGGTCATCAATGGAATACGCCAAGGATTAGAAGGTCTGATACGCAGTAATAAAAT
CACTGTATTGCATGGTCGAGGATCGCTCATCTCCTCTACAGAGGTCAGAGTCAAGGGTCAAGATACTTCAGTCACTAAAA
GTCAGTATATTATCATAGCTACAGGCTCAGAATCCCGCCCTTTCCCCGGTGTGCCATTTTCATCTCGCATTCTATGTTCT
ACCGGCATTTTAAATCTTACTGAGCTGCCTAAAAAACTCGCCATTATCGGCGGTGGTGTCATCGGTTGTGAATTTGCCTC
CCTATTTCATACTTTAGGAGTAGAAATTACGATCATAGAAGTCGCTGAGCAGATCCTTTCGGTAAATAACGCGGATATTT
CCAAAACCATGTTGGACAAATTCTCACGACAAGGAATCCGCATCATCACGAAAGCTTCGATTCATGCACTGGAAGATCTT
GGAGATCGTGTAAGAATCACTGTAAACGAACAAACAGAAGAATATGACTATGTTCTTGTCGCGATCGGACGTCAATTCAA
TACTACGGATATAGGTTTAGACAACGCAGGAGTCATTCGCGATAACCGTGGGATTATCCCTGTAGATGAGACGATGAGAA
CCAATGTCGCGAATATTTTCGCTATTGGAGATATCACAGGGAAATGGTTACTCGCTCATGTTGCCTCTCATCAGGGGATT
GTCGCAGGGAAAAATGCTGCCAGCCACAATGAAATTATGGATTATTCTGCAGTGCCTGCAGTCATTTTCACCCTCCCCGA
AGTTGCTATGGTGGGTCTTTCTTTGGAAGCTGCACAACAACAAGGTATCCCTGTGAAACTCACAAAGTTTCCTTTTAAAG
CTATTGGGAAAGCTGTCGCTATGGCAGAAGCTGACGGTTTTGCAGCGATCATCAGCCACGAGACTACACAACAAATTCTA
GGAGCCTATGTTGTCGGTCCACACGCAGCATCATTAATTGCAGAAATGACTCTAGCTGTACGTAATGAACTTACATTACC
TTGTATCTATGAAACTATACACGCTCATCCAACACTTGCTGAGGTTTGGGCTGAGAGTGCGTTATTAGCAACAAATCACC
CTCTTCATTTACCTCCTCCAAAACTATGA

Upstream 100 bases:

>100_bases
CACAAAGCTCCTCATAAAATCATACTAAGACCTAGCGTCTTAAAAAACATCTTCTTAAGGACTTTTAAATTTCGATACTT
ACGAGAATTGGGGACAAAAT

Downstream 100 bases:

>100_bases
ATGACACTCCTAATGACATACAAAAACCCAAACAAGGGAAACGTTTTTCAGAACGTCTCCCTCACTGGCTAAGGCAAGCT
TTACCCAAAGGCTCAGTCTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSTDFDCVVIGAGPGGYVAAITAAQQGLKTALIEEQQAGGTCLNRGCIPSKALLVGAGIVSQIKHANQFGIHIDGYSIDY
PAMVQRKNTVINGIRQGLEGLIRSNKITVLHGRGSLISSTEVRVKGQDTSVTKSQYIIIATGSESRPFPGVPFSSRILCS
TGILNLTELPKKLAIIGGGVIGCEFASLFHTLGVEITIIEVAEQILSVNNADISKTMLDKFSRQGIRIITKASIHALEDL
GDRVRITVNEQTEEYDYVLVAIGRQFNTTDIGLDNAGVIRDNRGIIPVDETMRTNVANIFAIGDITGKWLLAHVASHQGI
VAGKNAASHNEIMDYSAVPAVIFTLPEVAMVGLSLEAAQQQGIPVKLTKFPFKAIGKAVAMAEADGFAAIISHETTQQIL
GAYVVGPHAASLIAEMTLAVRNELTLPCIYETIHAHPTLAEVWAESALLATNHPLHLPPPKL

Sequences:

>Translated_462_residues
MSTDFDCVVIGAGPGGYVAAITAAQQGLKTALIEEQQAGGTCLNRGCIPSKALLVGAGIVSQIKHANQFGIHIDGYSIDY
PAMVQRKNTVINGIRQGLEGLIRSNKITVLHGRGSLISSTEVRVKGQDTSVTKSQYIIIATGSESRPFPGVPFSSRILCS
TGILNLTELPKKLAIIGGGVIGCEFASLFHTLGVEITIIEVAEQILSVNNADISKTMLDKFSRQGIRIITKASIHALEDL
GDRVRITVNEQTEEYDYVLVAIGRQFNTTDIGLDNAGVIRDNRGIIPVDETMRTNVANIFAIGDITGKWLLAHVASHQGI
VAGKNAASHNEIMDYSAVPAVIFTLPEVAMVGLSLEAAQQQGIPVKLTKFPFKAIGKAVAMAEADGFAAIISHETTQQIL
GAYVVGPHAASLIAEMTLAVRNELTLPCIYETIHAHPTLAEVWAESALLATNHPLHLPPPKL
>Mature_461_residues
STDFDCVVIGAGPGGYVAAITAAQQGLKTALIEEQQAGGTCLNRGCIPSKALLVGAGIVSQIKHANQFGIHIDGYSIDYP
AMVQRKNTVINGIRQGLEGLIRSNKITVLHGRGSLISSTEVRVKGQDTSVTKSQYIIIATGSESRPFPGVPFSSRILCST
GILNLTELPKKLAIIGGGVIGCEFASLFHTLGVEITIIEVAEQILSVNNADISKTMLDKFSRQGIRIITKASIHALEDLG
DRVRITVNEQTEEYDYVLVAIGRQFNTTDIGLDNAGVIRDNRGIIPVDETMRTNVANIFAIGDITGKWLLAHVASHQGIV
AGKNAASHNEIMDYSAVPAVIFTLPEVAMVGLSLEAAQQQGIPVKLTKFPFKAIGKAVAMAEADGFAAIISHETTQQILG
AYVVGPHAASLIAEMTLAVRNELTLPCIYETIHAHPTLAEVWAESALLATNHPLHLPPPKL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=36.9330453563715, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=26.5086206896552, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=27.3127753303965, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=26.8722466960352, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=26.8722466960352, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=26.8722466960352, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=26.8722466960352, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI291045266, Length=430, Percent_Identity=26.5116279069767, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=26.0593220338983, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=25, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1786307, Length=471, Percent_Identity=36.3057324840764, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=30.2222222222222, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=29.8507462686567, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=27.3781902552204, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=37.2294372294372, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=28.6624203821656, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71983429, Length=451, Percent_Identity=25.4988913525499, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=451, Percent_Identity=25.4988913525499, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=431, Percent_Identity=22.969837587007, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=246, Percent_Identity=28.4552845528455, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=36.0934182590234, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=28.6937901498929, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=25.7928118393235, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=39.2156862745098, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=29.8969072164948, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=29.8969072164948, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=29.8969072164948, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=28.3018867924528, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49217; Mature: 49086

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDFDCVVIGAGPGGYVAAITAAQQGLKTALIEEQQAGGTCLNRGCIPSKALLVGAGIV
CCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
SQIKHANQFGIHIDGYSIDYPAMVQRKNTVINGIRQGLEGLIRSNKITVLHGRGSLISST
HHHHCCCCCEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECC
EVRVKGQDTSVTKSQYIIIATGSESRPFPGVPFSSRILCSTGILNLTELPKKLAIIGGGV
EEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHCCHHHEEECCCH
IGCEFASLFHTLGVEITIIEVAEQILSVNNADISKTMLDKFSRQGIRIITKASIHALEDL
HHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCC
GDRVRITVNEQTEEYDYVLVAIGRQFNTTDIGLDNAGVIRDNRGIIPVDETMRTNVANIF
CCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEECCCHHHHCCCEEEE
AIGDITGKWLLAHVASHQGIVAGKNAASHNEIMDYSAVPAVIFTLPEVAMVGLSLEAAQQ
EEECCCHHHHHHHHHCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHCCCHHHHHH
QGIPVKLTKFPFKAIGKAVAMAEADGFAAIISHETTQQILGAYVVGPHAASLIAEMTLAV
CCCCEEEECCCHHHHHHHHHEECCCCEEEEECCHHHHHHHHEEEECCHHHHHHHHHHHHH
RNELTLPCIYETIHAHPTLAEVWAESALLATNHPLHLPPPKL
HCCCCCHHHHHHHHCCCCHHHHHHCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
STDFDCVVIGAGPGGYVAAITAAQQGLKTALIEEQQAGGTCLNRGCIPSKALLVGAGIV
CCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
SQIKHANQFGIHIDGYSIDYPAMVQRKNTVINGIRQGLEGLIRSNKITVLHGRGSLISST
HHHHCCCCCEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECC
EVRVKGQDTSVTKSQYIIIATGSESRPFPGVPFSSRILCSTGILNLTELPKKLAIIGGGV
EEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHCCHHHEEECCCH
IGCEFASLFHTLGVEITIIEVAEQILSVNNADISKTMLDKFSRQGIRIITKASIHALEDL
HHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCC
GDRVRITVNEQTEEYDYVLVAIGRQFNTTDIGLDNAGVIRDNRGIIPVDETMRTNVANIF
CCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEECCCHHHHCCCEEEE
AIGDITGKWLLAHVASHQGIVAGKNAASHNEIMDYSAVPAVIFTLPEVAMVGLSLEAAQQ
EEECCCHHHHHHHHHCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHCCCHHHHHH
QGIPVKLTKFPFKAIGKAVAMAEADGFAAIISHETTQQILGAYVVGPHAASLIAEMTLAV
CCCCEEEECCCHHHHHHHHHEECCCCEEEEECCHHHHHHHHEEEECCHHHHHHHHHHHHH
RNELTLPCIYETIHAHPTLAEVWAESALLATNHPLHLPPPKL
HCCCCCHHHHHHHHCCCCHHHHHHCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]