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The map label for this gene is nqrF [H]

Identifier: 62185454

GI number: 62185454

Start: 988228

End: 989523

Strand: Direct

Name: nqrF [H]

Synonym: CAB851

Alternate gene names: 62185454

Gene position: 988228-989523 (Clockwise)

Preceding gene: 62185453

Following gene: 62185457

Centisome position: 86.36

GC content: 39.35

Gene sequence:

>1296_bases
ATGACTTGGCTTTCAGGCCTATATTTTATCAGCATTGCTAGTTTAGTATTTTGTGTTATAGGTTTGATACTTTCTGGAAT
CATTCTTATTGCTCATAAATTTCTAGTTAAAATTCATCCTTGCAAGCTAAAAATTAACGATGACGACACTCTTACAAAAA
CTGTAGATAGTGGTCGTACCTTATTGTCTTCTCTATTAGATTCGGGTATTCCGATCCCTTCTCCATGTGGAGGCAAGGCT
ACGTGTAAACAATGTAAGGTAAAAATTGTTAAAGACGCTGATCCACCACTAGAAACAGACCGCGCAACTTTTTCCAAACA
ACAGTTAGAACACGGCTGGCGTCTTTCCTGCCAAACCAAGGTCCAACACGACATGTGTTTGGAAATCGAGGAGCGTTATT
TACATGCTTCTTCTTGGGAAGGTACGGTCGTTTCTAACGATAACGTCGCTACCTTTATAAAAGAGCTTGTTGTTTCTATT
GATCCTGCGCATCCTATTCCCTTTAAGCCTGGGGGGTATCTACAAATTCGTGTTCCTGCATATAAAACGAATACTTCCGA
TTGGAAGCAAACTATGGCTCCCGAATATCATAGTGATTGGGAACGGTTTAATTTATTTGGCCGGATTATAGATAACAGCC
TTTTGGAGCCAGATTCTGCTAATAAAGCCTATTCTCTCGCATCTTATCCTGCAGAATTGCCGATTATTAAATTCAATGTT
CGTATTGCGACACCTCCTTTTATTAACAATGCGCCTAATCCGGGTATCCCCTGGGGGGTATGCTCATCCTATATCTTCTC
GCTACAACCTGGGGATAAGATTACTGTATCTGGTCCTTATGGCGAATCCTTCATGAAAGAAAACAACCGCCCGTTAATTT
TTCTAATTGGTGGTGCGGGATCTTCATTTGGTAGAAGTCATATTTTAGATCTTTTGTTGGATAAACATTCTACAAGAGAG
ATCACTCTGTGGTACGGAGCTCGCTCTCTAAAAGAAAATATCTATCAAGAAGAATATGAAAAATTAGATAAGGATTTTCC
TAACTTCCATTACCATTTAGTGTTATCGGAGCCTCTTCCTGAAGACATTGCCGCAGGTTGGGATAAGGATGATCCTGAGA
AAACGAATTTCTTATTCCAAGCTTTTGAGCTTGGTCAACTCAGCAAACTTAGCAATCCTGAAGATTATCTATATTATGTA
TGTGGTCCACCACTACACAACAGTAGTATCCTGAAATTACTTGAAAATTATGGAGTTGAAAGATCTTCCATAATTCTCGA
TGATTTTGGCAGCTAG

Upstream 100 bases:

>100_bases
TTACTGGCTACGATATTAGAGAAATCTACATTGATTTTTTATAAGCCAAGTTATAACTTGACACCATAAGCTATTCACGA
AGTCGAGGATTTTTAGGATC

Downstream 100 bases:

>100_bases
TTACTCAAGAGATGTTTTCTCGCTATAGATATTCTCAAGATTTTATCGATTACGAGTTCTCAGCATTCGGACTAAAGACG
CAATGTATATTATCGTAGCG

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTWLSGLYFISIASLVFCVIGLILSGIILIAHKFLVKIHPCKLKINDDDTLTKTVDSGRTLLSSLLDSGIPIPSPCGGKA
TCKQCKVKIVKDADPPLETDRATFSKQQLEHGWRLSCQTKVQHDMCLEIEERYLHASSWEGTVVSNDNVATFIKELVVSI
DPAHPIPFKPGGYLQIRVPAYKTNTSDWKQTMAPEYHSDWERFNLFGRIIDNSLLEPDSANKAYSLASYPAELPIIKFNV
RIATPPFINNAPNPGIPWGVCSSYIFSLQPGDKITVSGPYGESFMKENNRPLIFLIGGAGSSFGRSHILDLLLDKHSTRE
ITLWYGARSLKENIYQEEYEKLDKDFPNFHYHLVLSEPLPEDIAAGWDKDDPEKTNFLFQAFELGQLSKLSNPEDYLYYV
CGPPLHNSSILKLLENYGVERSSIILDDFGS

Sequences:

>Translated_431_residues
MTWLSGLYFISIASLVFCVIGLILSGIILIAHKFLVKIHPCKLKINDDDTLTKTVDSGRTLLSSLLDSGIPIPSPCGGKA
TCKQCKVKIVKDADPPLETDRATFSKQQLEHGWRLSCQTKVQHDMCLEIEERYLHASSWEGTVVSNDNVATFIKELVVSI
DPAHPIPFKPGGYLQIRVPAYKTNTSDWKQTMAPEYHSDWERFNLFGRIIDNSLLEPDSANKAYSLASYPAELPIIKFNV
RIATPPFINNAPNPGIPWGVCSSYIFSLQPGDKITVSGPYGESFMKENNRPLIFLIGGAGSSFGRSHILDLLLDKHSTRE
ITLWYGARSLKENIYQEEYEKLDKDFPNFHYHLVLSEPLPEDIAAGWDKDDPEKTNFLFQAFELGQLSKLSNPEDYLYYV
CGPPLHNSSILKLLENYGVERSSIILDDFGS
>Mature_430_residues
TWLSGLYFISIASLVFCVIGLILSGIILIAHKFLVKIHPCKLKINDDDTLTKTVDSGRTLLSSLLDSGIPIPSPCGGKAT
CKQCKVKIVKDADPPLETDRATFSKQQLEHGWRLSCQTKVQHDMCLEIEERYLHASSWEGTVVSNDNVATFIKELVVSID
PAHPIPFKPGGYLQIRVPAYKTNTSDWKQTMAPEYHSDWERFNLFGRIIDNSLLEPDSANKAYSLASYPAELPIIKFNVR
IATPPFINNAPNPGIPWGVCSSYIFSLQPGDKITVSGPYGESFMKENNRPLIFLIGGAGSSFGRSHILDLLLDKHSTREI
TLWYGARSLKENIYQEEYEKLDKDFPNFHYHLVLSEPLPEDIAAGWDKDDPEKTNFLFQAFELGQLSKLSNPEDYLYYVC
GPPLHNSSILKLLENYGVERSSIILDDFGS

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 48396; Mature: 48264

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLSGLYFISIASLVFCVIGLILSGIILIAHKFLVKIHPCKLKINDDDTLTKTVDSGRT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEECCCCHHHHHHHHHHH
LLSSLLDSGIPIPSPCGGKATCKQCKVKIVKDADPPLETDRATFSKQQLEHGWRLSCQTK
HHHHHHHCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECC
VQHDMCLEIEERYLHASSWEGTVVSNDNVATFIKELVVSIDPAHPIPFKPGGYLQIRVPA
CCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
YKTNTSDWKQTMAPEYHSDWERFNLFGRIIDNSLLEPDSANKAYSLASYPAELPIIKFNV
EECCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEE
RIATPPFINNAPNPGIPWGVCSSYIFSLQPGDKITVSGPYGESFMKENNRPLIFLIGGAG
EEECCCCCCCCCCCCCCHHHHHHHEEEECCCCEEEEECCCCHHHHHCCCCCEEEEEECCC
SSFGRSHILDLLLDKHSTREITLWYGARSLKENIYQEEYEKLDKDFPNFHYHLVLSEPLP
CCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
EDIAAGWDKDDPEKTNFLFQAFELGQLSKLSNPEDYLYYVCGPPLHNSSILKLLENYGVE
HHHHCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
RSSIILDDFGS
CCEEEECCCCC
>Mature Secondary Structure 
TWLSGLYFISIASLVFCVIGLILSGIILIAHKFLVKIHPCKLKINDDDTLTKTVDSGRT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEECCCCHHHHHHHHHHH
LLSSLLDSGIPIPSPCGGKATCKQCKVKIVKDADPPLETDRATFSKQQLEHGWRLSCQTK
HHHHHHHCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECC
VQHDMCLEIEERYLHASSWEGTVVSNDNVATFIKELVVSIDPAHPIPFKPGGYLQIRVPA
CCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
YKTNTSDWKQTMAPEYHSDWERFNLFGRIIDNSLLEPDSANKAYSLASYPAELPIIKFNV
EECCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEE
RIATPPFINNAPNPGIPWGVCSSYIFSLQPGDKITVSGPYGESFMKENNRPLIFLIGGAG
EEECCCCCCCCCCCCCCHHHHHHHEEEECCCCEEEEECCCCHHHHHCCCCCEEEEEECCC
SSFGRSHILDLLLDKHSTREITLWYGARSLKENIYQEEYEKLDKDFPNFHYHLVLSEPLP
CCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
EDIAAGWDKDDPEKTNFLFQAFELGQLSKLSNPEDYLYYVCGPPLHNSSILKLLENYGVE
HHHHCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
RSSIILDDFGS
CCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]