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The map label for this gene is aroA

Identifier: 62185303

GI number: 62185303

Start: 800409

End: 801746

Strand: Direct

Name: aroA

Synonym: CAB690

Alternate gene names: 62185303

Gene position: 800409-801746 (Clockwise)

Preceding gene: 62185302

Following gene: 62185304

Centisome position: 69.94

GC content: 43.5

Gene sequence:

>1338_bases
ATGCTTGCTTATAAGATTTCTCCCTCCTCTATATCTGGACGGGTTTCCGTCCCTCCGTCGAAGTCTCATACTCTACGAGC
TATCTTTTGGGCTTCAGTAGCACAAGGTACGTCCATAATACATCATGCTCTTGCCTCTCCAGACTCAGAAGCTATGATCC
AAGCCTGCAAGCAGTTGGGCGCAAAAATTCTTAAAAAATCTACACATCTTGAAATTACGGGAACACCCCACCTAATACTT
CCCAAAAACACTACCATCAATGCAGGCAGTTCTGGCATCGTTTTTCGTTTCTTTACAGCACTGGCAGCAATCTTTTCCGA
GAAGATCACAATTACAGAATCTTCACAATTACAACGGCGTCCCATAGCTCCCTTAATCCAAGCTCTAGAAAATTTCGGGG
CTACCTTTTCCTATGAAGGAGAGCCCTATTCTCTGCCCTTCTCTGTTTTAGGGCCCATGTCTTCCGGGTATACAGAAGTT
ATAGGCGAGGATTCCCAATACGCCTCTGCTTTGGCTATAGCCTGTTCTTTAGCCGAAGGACCTTTTTCATTTACTATCCT
CAATCCCAAAGAACGCCCTTGGTTTGCTCTTACATTGTGGTGGCTGGATTTCCTCGGTATTCCCTATGCACAATCTGAGG
ATACGTACTCGTTTGAAGGGAAAACTCGCCCCCAAGCATTTTCCTATACTGTCGGGGGTGATTTTAGCAGCGCTGCGTTC
CTTGCAGCTGCTGCCTTACTTTCTCAATCTCCTCATCCTACCTACTTAGAAAATCTCAATATCGACGATATCCAAGGCGA
TAAGGAACTCTTTTTCCTTCTTAAAAAACTTGGAGCAAATATTACTTTTGAAAACAACACCATCATTATTTTCCCTTCCA
CATTTTCAGGAGGAAATATTGATATGGATCCTTTTATTGACGCTCTTCCTATTCTCGCTGTTCTCTGCTGTTTTGCTACT
TCCTCCTCGTATCTATATAACGCTCGAAGTGCTAAAAATAAAGAAAGCGACCGCATCATTGCTATCACTCAAGAACTACA
GAAAATGGGAGCTTGTATCCAACCGTGTCACGACGGTTTGCTCATCAATCCCAGCCCATTATATGGAGCTTCTATGCATT
CTCATAAAGATCATAGAATTGCCATGGCCCTGTCTATAGCAGCGATGCATGCTTCTGGGGATAGCATTATCTATGATACC
GATTGCGTAAAAAAAACTTTCCCGAATTTCATCCAGATTCTAAATTCCTTGCATACGAATATCCAGGAACATTATGAACA
TATTTCTCTGCGGCCTGCCGACAGTAGGCAAGACGTTGTTTGGCAAGGCTCTCGCTAA

Upstream 100 bases:

>100_bases
AAACATGTAAGATTTAGTCTTCAAAAATAGAGTATATTTTCTTGGACGATAAATTAGTGTTCAGGCAAGCTTTTTGGAAA
ACAAAAGTAAGTTAGCCAAA

Downstream 100 bases:

>100_bases
ATATCTTTCGGTGCCTTTCTTTGATGTCGATGATTTAATCGTAAGTAACTACGGCAATAAGCTATATCCCTCGGCCTGCG
AAATATTCCAGGCTATTGGA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MLAYKISPSSISGRVSVPPSKSHTLRAIFWASVAQGTSIIHHALASPDSEAMIQACKQLGAKILKKSTHLEITGTPHLIL
PKNTTINAGSSGIVFRFFTALAAIFSEKITITESSQLQRRPIAPLIQALENFGATFSYEGEPYSLPFSVLGPMSSGYTEV
IGEDSQYASALAIACSLAEGPFSFTILNPKERPWFALTLWWLDFLGIPYAQSEDTYSFEGKTRPQAFSYTVGGDFSSAAF
LAAAALLSQSPHPTYLENLNIDDIQGDKELFFLLKKLGANITFENNTIIIFPSTFSGGNIDMDPFIDALPILAVLCCFAT
SSSYLYNARSAKNKESDRIIAITQELQKMGACIQPCHDGLLINPSPLYGASMHSHKDHRIAMALSIAAMHASGDSIIYDT
DCVKKTFPNFIQILNSLHTNIQEHYEHISLRPADSRQDVVWQGSR

Sequences:

>Translated_445_residues
MLAYKISPSSISGRVSVPPSKSHTLRAIFWASVAQGTSIIHHALASPDSEAMIQACKQLGAKILKKSTHLEITGTPHLIL
PKNTTINAGSSGIVFRFFTALAAIFSEKITITESSQLQRRPIAPLIQALENFGATFSYEGEPYSLPFSVLGPMSSGYTEV
IGEDSQYASALAIACSLAEGPFSFTILNPKERPWFALTLWWLDFLGIPYAQSEDTYSFEGKTRPQAFSYTVGGDFSSAAF
LAAAALLSQSPHPTYLENLNIDDIQGDKELFFLLKKLGANITFENNTIIIFPSTFSGGNIDMDPFIDALPILAVLCCFAT
SSSYLYNARSAKNKESDRIIAITQELQKMGACIQPCHDGLLINPSPLYGASMHSHKDHRIAMALSIAAMHASGDSIIYDT
DCVKKTFPNFIQILNSLHTNIQEHYEHISLRPADSRQDVVWQGSR
>Mature_445_residues
MLAYKISPSSISGRVSVPPSKSHTLRAIFWASVAQGTSIIHHALASPDSEAMIQACKQLGAKILKKSTHLEITGTPHLIL
PKNTTINAGSSGIVFRFFTALAAIFSEKITITESSQLQRRPIAPLIQALENFGATFSYEGEPYSLPFSVLGPMSSGYTEV
IGEDSQYASALAIACSLAEGPFSFTILNPKERPWFALTLWWLDFLGIPYAQSEDTYSFEGKTRPQAFSYTVGGDFSSAAF
LAAAALLSQSPHPTYLENLNIDDIQGDKELFFLLKKLGANITFENNTIIIFPSTFSGGNIDMDPFIDALPILAVLCCFAT
SSSYLYNARSAKNKESDRIIAITQELQKMGACIQPCHDGLLINPSPLYGASMHSHKDHRIAMALSIAAMHASGDSIIYDT
DCVKKTFPNFIQILNSLHTNIQEHYEHISLRPADSRQDVVWQGSR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=419, Percent_Identity=29.3556085918854, Blast_Score=141, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320332, Length=463, Percent_Identity=26.133909287257, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_CHLAB (Q5L5F4)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220088.1
- HSSP:   P22487
- GeneID:   3337259
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB690
- HOGENOM:   HBG646626
- OMA:   SSQFITS
- ProtClustDB:   PRK02427
- BioCyc:   CABO218497:CAB690-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 48700; Mature: 48700

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAYKISPSSISGRVSVPPSKSHTLRAIFWASVAQGTSIIHHALASPDSEAMIQACKQLG
CEEEEECCCCCCCEEECCCCCCCEEEHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHH
AKILKKSTHLEITGTPHLILPKNTTINAGSSGIVFRFFTALAAIFSEKITITESSQLQRR
HHHHCCCCEEEEECCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHCC
PIAPLIQALENFGATFSYEGEPYSLPFSVLGPMSSGYTEVIGEDSQYASALAIACSLAEG
CHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHEECCC
PFSFTILNPKERPWFALTLWWLDFLGIPYAQSEDTYSFEGKTRPQAFSYTVGGDFSSAAF
CEEEEEECCCCCCEEEEHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCCHHHHH
LAAAALLSQSPHPTYLENLNIDDIQGDKELFFLLKKLGANITFENNTIIIFPSTFSGGNI
HHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCC
DMDPFIDALPILAVLCCFATSSSYLYNARSAKNKESDRIIAITQELQKMGACIQPCHDGL
CHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCE
LINPSPLYGASMHSHKDHRIAMALSIAAMHASGDSIIYDTDCVKKTFPNFIQILNSLHTN
EECCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
IQEHYEHISLRPADSRQDVVWQGSR
HHHHHHHCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MLAYKISPSSISGRVSVPPSKSHTLRAIFWASVAQGTSIIHHALASPDSEAMIQACKQLG
CEEEEECCCCCCCEEECCCCCCCEEEHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHH
AKILKKSTHLEITGTPHLILPKNTTINAGSSGIVFRFFTALAAIFSEKITITESSQLQRR
HHHHCCCCEEEEECCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHCC
PIAPLIQALENFGATFSYEGEPYSLPFSVLGPMSSGYTEVIGEDSQYASALAIACSLAEG
CHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHEECCC
PFSFTILNPKERPWFALTLWWLDFLGIPYAQSEDTYSFEGKTRPQAFSYTVGGDFSSAAF
CEEEEEECCCCCCEEEEHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCCHHHHH
LAAAALLSQSPHPTYLENLNIDDIQGDKELFFLLKKLGANITFENNTIIIFPSTFSGGNI
HHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCC
DMDPFIDALPILAVLCCFATSSSYLYNARSAKNKESDRIIAITQELQKMGACIQPCHDGL
CHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCE
LINPSPLYGASMHSHKDHRIAMALSIAAMHASGDSIIYDTDCVKKTFPNFIQILNSLHTN
EECCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
IQEHYEHISLRPADSRQDVVWQGSR
HHHHHHHCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA