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The map label for this gene is bioB

Identifier: 62185298

GI number: 62185298

Start: 795683

End: 796678

Strand: Direct

Name: bioB

Synonym: CAB685

Alternate gene names: 62185298

Gene position: 795683-796678 (Clockwise)

Preceding gene: 62185297

Following gene: 62185299

Centisome position: 69.53

GC content: 42.47

Gene sequence:

>996_bases
ATGGAAACACACTCTGAATTATGGTCATTAGAAGCTATTAAAGAAGTATATAACACTCCTATTTTTGAGTTAATCCATAG
GGCGAACGCTATATTAAGAAGTAATTTTCCTCACTCCGAATTACAGACATGTTATCTTGTGTCGATAAAAACAGGTGGTT
GCACTGAAGATTGCGCTTATTGCGCACAGTCTTCACGTTACCAAACACATGTTAAACCAGAACCAATGATGAAAATCACA
GAAGTTTTGGATAGGGCAAAACAGGCTATACGTGCTGGAGCCACGCGCGTATGTCTTGGAGCTGCTTGGAGAGAAGTCAA
AGATAACCATCAATTTGATCGCACGTTAGAAATGATCAAAGGCATTACCGATATGGGTGCTGAGGTATGTTGTACTCTTG
GTATGCTCACTCCAAGTCAAGCAGAAAAGCTCTTTGAAGCCGGCCTCTATGCTTATAATCACAACTTAGATTCTTCTGAA
GGTTTTTACAAGACAATCATTACCACACGCAAATATGAAGATCGTTTACGCACTTTGGATATCGTTGAAAAATCTGGACT
TCATGTTTGTTGTGGTGGGATTATTGGCATGGGAGAGACTGTTGAAGATCGTGTCGAGCTGCTTCATAATCTCGCACGTC
GAGAACGTATGCCAGAATCGGTACCTGTCAATGTGCTTTGGCCGATAAAAGGAACTCCCCTATACGATCAAGCATCGATA
TCTTTTTGGGAGATCTTACGTACGATAGCTACAGCACGTATTGTGTTTCCCCAATCCATGGTACGCCTGGCCGCAGGACG
TGCTTTTCTTTCTGTGGAACAACAAACTCTATGTTTCATTGCCGGAGCGAATTCGATTTTCTATGGTGAGAAACTCCTCA
CTGTAGATAACAACGATATGGATGCAGACACTGCCATGTTGAATTTACTTGGCATGCGCCACCGTCCATCATTTTCAATG
GAAAGAGGGCAACCATGCCAATCTGTGACCTGTTAG

Upstream 100 bases:

>100_bases
GACTTCCAAAAACCCGTTGTTATTGACTTTCTAGGGATTCTATGTTAACAATTTTCCCCTTTGTCTACATTGTCAACCTT
TTTCTAGGAAAGCATTACTT

Downstream 100 bases:

>100_bases
AACAAAGATTAGAAAAACAAAAACAGAAAAAGATTCACCGCACATTAAAAACTGTCTCTGCTTCTATAGATTTCACGTCT
AATGATTATCTTGGCTTTGC

Product: putative biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
METHSELWSLEAIKEVYNTPIFELIHRANAILRSNFPHSELQTCYLVSIKTGGCTEDCAYCAQSSRYQTHVKPEPMMKIT
EVLDRAKQAIRAGATRVCLGAAWREVKDNHQFDRTLEMIKGITDMGAEVCCTLGMLTPSQAEKLFEAGLYAYNHNLDSSE
GFYKTIITTRKYEDRLRTLDIVEKSGLHVCCGGIIGMGETVEDRVELLHNLARRERMPESVPVNVLWPIKGTPLYDQASI
SFWEILRTIATARIVFPQSMVRLAAGRAFLSVEQQTLCFIAGANSIFYGEKLLTVDNNDMDADTAMLNLLGMRHRPSFSM
ERGQPCQSVTC

Sequences:

>Translated_331_residues
METHSELWSLEAIKEVYNTPIFELIHRANAILRSNFPHSELQTCYLVSIKTGGCTEDCAYCAQSSRYQTHVKPEPMMKIT
EVLDRAKQAIRAGATRVCLGAAWREVKDNHQFDRTLEMIKGITDMGAEVCCTLGMLTPSQAEKLFEAGLYAYNHNLDSSE
GFYKTIITTRKYEDRLRTLDIVEKSGLHVCCGGIIGMGETVEDRVELLHNLARRERMPESVPVNVLWPIKGTPLYDQASI
SFWEILRTIATARIVFPQSMVRLAAGRAFLSVEQQTLCFIAGANSIFYGEKLLTVDNNDMDADTAMLNLLGMRHRPSFSM
ERGQPCQSVTC
>Mature_331_residues
METHSELWSLEAIKEVYNTPIFELIHRANAILRSNFPHSELQTCYLVSIKTGGCTEDCAYCAQSSRYQTHVKPEPMMKIT
EVLDRAKQAIRAGATRVCLGAAWREVKDNHQFDRTLEMIKGITDMGAEVCCTLGMLTPSQAEKLFEAGLYAYNHNLDSSE
GFYKTIITTRKYEDRLRTLDIVEKSGLHVCCGGIIGMGETVEDRVELLHNLARRERMPESVPVNVLWPIKGTPLYDQASI
SFWEILRTIATARIVFPQSMVRLAAGRAFLSVEQQTLCFIAGANSIFYGEKLLTVDNNDMDADTAMLNLLGMRHRPSFSM
ERGQPCQSVTC

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=47.9099678456592, Blast_Score=307, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6321725, Length=325, Percent_Identity=46.4615384615385, Blast_Score=308, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CHLAB (Q5L5F9)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220083.1
- HSSP:   P12996
- ProteinModelPortal:   Q5L5F9
- SMR:   Q5L5F9
- GeneID:   3337273
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB685
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q5L5F9
- ProtClustDB:   CLSK2300100
- BioCyc:   CABO218497:CAB685-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37259; Mature: 37259

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHSELWSLEAIKEVYNTPIFELIHRANAILRSNFPHSELQTCYLVSIKTGGCTEDCAY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCEEEEEEEECCCCCHHHHH
CAQSSRYQTHVKPEPMMKITEVLDRAKQAIRAGATRVCLGAAWREVKDNHQFDRTLEMIK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHH
GITDMGAEVCCTLGMLTPSQAEKLFEAGLYAYNHNLDSSEGFYKTIITTRKYEDRLRTLD
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHH
IVEKSGLHVCCGGIIGMGETVEDRVELLHNLARRERMPESVPVNVLWPIKGTPLYDQASI
HHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCH
SFWEILRTIATARIVFPQSMVRLAAGRAFLSVEQQTLCFIAGANSIFYGEKLLTVDNNDM
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCEEEEEECCCCEEECCEEEEECCCCC
DADTAMLNLLGMRHRPSFSMERGQPCQSVTC
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METHSELWSLEAIKEVYNTPIFELIHRANAILRSNFPHSELQTCYLVSIKTGGCTEDCAY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCEEEEEEEECCCCCHHHHH
CAQSSRYQTHVKPEPMMKITEVLDRAKQAIRAGATRVCLGAAWREVKDNHQFDRTLEMIK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHH
GITDMGAEVCCTLGMLTPSQAEKLFEAGLYAYNHNLDSSEGFYKTIITTRKYEDRLRTLD
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHH
IVEKSGLHVCCGGIIGMGETVEDRVELLHNLARRERMPESVPVNVLWPIKGTPLYDQASI
HHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCH
SFWEILRTIATARIVFPQSMVRLAAGRAFLSVEQQTLCFIAGANSIFYGEKLLTVDNNDM
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCEEEEEECCCCEEECCEEEEECCCCC
DADTAMLNLLGMRHRPSFSMERGQPCQSVTC
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA