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The map label for this gene is lepB [C]

Identifier: 62185246

GI number: 62185246

Start: 724920

End: 726812

Strand: Direct

Name: lepB [C]

Synonym: CAB633

Alternate gene names: 62185246

Gene position: 724920-726812 (Clockwise)

Preceding gene: 62185245

Following gene: 62185248

Centisome position: 63.35

GC content: 39.36

Gene sequence:

>1893_bases
ATGAAAGATCGGTTTTCTTTAAATAAAAGTCGTCAAGTACTTCACTCAACATATAAATTACTCAAAAGTAAAAAACTTGC
TCAAGATCCCGAATCTAAACAAGAGCTACAAAAGTTATTAGAACAACTTGAAGAAGCCATTTTTCAACAAGATCAGGAAG
CAGCTAGTCAACTCGCACAACAAGCTCAGCAATTCAGCAAGCGGTACCCCGCTTCTTTTGCAAAAAAATCATGGGAACTG
ACTAAAGCCATTCTTTTTGCTGCCCTAGCAGCTTTTCTTATTCGCCAATTTTGGTTTGAACTTTACGAAGTTCCTACAGG
ATCCATGCGTCCAACTATTTTAGAACAAGACCGCATGATTGTTTCTAAAACTACTTTCGGCTTACATTTCCCGTTTAAGA
AAAAGCCTTGGGGATTTCGCCCGGAAGCAGTGACTCGTGGTGGTCTTGTAGTCTTTACCGTTGGTGATCTTCCAATTCCC
AACTCTGATACTAAGTATTTTGGATTCATTCCAGGGAAAAAACGTTATATTAAGCGCTGTATGGGGAAGCCAGGCGACAC
CCTCTATTTTTATGGTGGTAAAATTTATGGCTTAGATAAAGAAGGTGCTGTTATTCACTTTCCCAATGATTTTGGTCTCG
AGCATCTCTATCACGTTCCTTACATATCTTTTGATGGGTCAGTCGAGATCGTAAATAGCAATAAAACTACCGCGTATTTT
AAACAAATGTACCAACCGTGTGGGAAACTTTCCTTACCTCAAGAAGGTCCCTACGGCCAATTTTTCCATCAAGGTGTTTG
GCACAATGACACCCCAAGTGCATTAAAAAAACCCCACTCTTCTCCTGTGAGTTATGCTGATCTTTTTGGTATAGGCAACT
ATGCCATGGTGCGTATTCTGACGCATAAACAAGCGAGCTTGTATCACACATTACCTAATGCAACAACTCAGGCATACTTA
GAGATATCTCATACTCCGAACGTTTCCTATCCCACACCGAATCTTCAGCATTATAACAATCAGATTGTTCCTACAATACA
ACCGATGAAAACACTTCTTCCCTTACGTCAGGAACATATTCACCTGATTAGGAATAATCTAACCACATCGCGCTTTGTGA
TTTCGGATGGTGTTGCTTGTAAATACCAACCTTTTTCCACAGATCCCGAGGGAGCCGCCAAACTCTACGGTCTTCCTTTC
CCCGGGGTAGATGATGGTTGTTACGAGTATTCAAAGGGAGAAGCTTACAAAATCGGTTTTGGGGGTATGCGTTATAAACT
TAAACCTACGCATCCTTTAATGCAGTTAAATGATAATCAAGTAATTGATTTATTTAACTGTGGCATGAATTTTAGTTCTT
TCTTTATCCCAAAAAATCCCAAATACAATCCTTTGCCTAACCGTTATGCATTTTATAATCAGGGGAATCTTTATGTTATG
GACTCTCCTATTTTCATTAAAAATGATCCCGCTTTGCAAAAGTTTGTAGAATCTGAAAAAACGAAACAGGAAGAATCTAC
AGAAGATCGCCCTTATATAAGCTTTATTGACAGAGGCCCTCCTCCTCAAGATCCAGAACAATTCGCAGAGTTTATTCGCA
ATTTTGGAATACAAATTCCTGAGAACCACGTTTTGGTTTTAGGGGATAACTACCCTATGAGTGCTGATAGCAGAGAGTTC
GGCTTTGTACCTTTAGAAAACCTCTTAGGATCACCTTTATGGATTTTCTGGCCTTTGGGACATTTTGGACGTTTAAAAAA
TGTTCCTGCGCCAACTACACTGCCGGGATATTTAGTCAATGGTCTGGCTTTAGGATTCTTTGTTTATATATTTGGGTATA
TACGTTATCAACGACGCCATCGTTTGTTTCCCAAGAATCATAAGAAAAAATAA

Upstream 100 bases:

>100_bases
CCACAATTCCGATTAGGGAAATTCCCTTTTCGAACCTCCATATAGGATTATGGAGAAATTCAAACTCAGAAAGCAATTAA
GGGTTTCACTAGGCATCTTC

Downstream 100 bases:

>100_bases
AAAAGAAGACCCTCCTAACAGAGGATCTTCTTTTCTCTATGCAGCGAGAAAGCTAGAGCTTAATGTGGTCTAGCAGCTAC
TTCGATAGCGATAACTGTGG

Product: signal peptidase I

Products: NA

Alternate protein names: Signal Peptidase

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MKDRFSLNKSRQVLHSTYKLLKSKKLAQDPESKQELQKLLEQLEEAIFQQDQEAASQLAQQAQQFSKRYPASFAKKSWEL
TKAILFAALAAFLIRQFWFELYEVPTGSMRPTILEQDRMIVSKTTFGLHFPFKKKPWGFRPEAVTRGGLVVFTVGDLPIP
NSDTKYFGFIPGKKRYIKRCMGKPGDTLYFYGGKIYGLDKEGAVIHFPNDFGLEHLYHVPYISFDGSVEIVNSNKTTAYF
KQMYQPCGKLSLPQEGPYGQFFHQGVWHNDTPSALKKPHSSPVSYADLFGIGNYAMVRILTHKQASLYHTLPNATTQAYL
EISHTPNVSYPTPNLQHYNNQIVPTIQPMKTLLPLRQEHIHLIRNNLTTSRFVISDGVACKYQPFSTDPEGAAKLYGLPF
PGVDDGCYEYSKGEAYKIGFGGMRYKLKPTHPLMQLNDNQVIDLFNCGMNFSSFFIPKNPKYNPLPNRYAFYNQGNLYVM
DSPIFIKNDPALQKFVESEKTKQEESTEDRPYISFIDRGPPPQDPEQFAEFIRNFGIQIPENHVLVLGDNYPMSADSREF
GFVPLENLLGSPLWIFWPLGHFGRLKNVPAPTTLPGYLVNGLALGFFVYIFGYIRYQRRHRLFPKNHKKK

Sequences:

>Translated_630_residues
MKDRFSLNKSRQVLHSTYKLLKSKKLAQDPESKQELQKLLEQLEEAIFQQDQEAASQLAQQAQQFSKRYPASFAKKSWEL
TKAILFAALAAFLIRQFWFELYEVPTGSMRPTILEQDRMIVSKTTFGLHFPFKKKPWGFRPEAVTRGGLVVFTVGDLPIP
NSDTKYFGFIPGKKRYIKRCMGKPGDTLYFYGGKIYGLDKEGAVIHFPNDFGLEHLYHVPYISFDGSVEIVNSNKTTAYF
KQMYQPCGKLSLPQEGPYGQFFHQGVWHNDTPSALKKPHSSPVSYADLFGIGNYAMVRILTHKQASLYHTLPNATTQAYL
EISHTPNVSYPTPNLQHYNNQIVPTIQPMKTLLPLRQEHIHLIRNNLTTSRFVISDGVACKYQPFSTDPEGAAKLYGLPF
PGVDDGCYEYSKGEAYKIGFGGMRYKLKPTHPLMQLNDNQVIDLFNCGMNFSSFFIPKNPKYNPLPNRYAFYNQGNLYVM
DSPIFIKNDPALQKFVESEKTKQEESTEDRPYISFIDRGPPPQDPEQFAEFIRNFGIQIPENHVLVLGDNYPMSADSREF
GFVPLENLLGSPLWIFWPLGHFGRLKNVPAPTTLPGYLVNGLALGFFVYIFGYIRYQRRHRLFPKNHKKK
>Mature_630_residues
MKDRFSLNKSRQVLHSTYKLLKSKKLAQDPESKQELQKLLEQLEEAIFQQDQEAASQLAQQAQQFSKRYPASFAKKSWEL
TKAILFAALAAFLIRQFWFELYEVPTGSMRPTILEQDRMIVSKTTFGLHFPFKKKPWGFRPEAVTRGGLVVFTVGDLPIP
NSDTKYFGFIPGKKRYIKRCMGKPGDTLYFYGGKIYGLDKEGAVIHFPNDFGLEHLYHVPYISFDGSVEIVNSNKTTAYF
KQMYQPCGKLSLPQEGPYGQFFHQGVWHNDTPSALKKPHSSPVSYADLFGIGNYAMVRILTHKQASLYHTLPNATTQAYL
EISHTPNVSYPTPNLQHYNNQIVPTIQPMKTLLPLRQEHIHLIRNNLTTSRFVISDGVACKYQPFSTDPEGAAKLYGLPF
PGVDDGCYEYSKGEAYKIGFGGMRYKLKPTHPLMQLNDNQVIDLFNCGMNFSSFFIPKNPKYNPLPNRYAFYNQGNLYVM
DSPIFIKNDPALQKFVESEKTKQEESTEDRPYISFIDRGPPPQDPEQFAEFIRNFGIQIPENHVLVLGDNYPMSADSREF
GFVPLENLLGSPLWIFWPLGHFGRLKNVPAPTTLPGYLVNGLALGFFVYIFGYIRYQRRHRLFPKNHKKK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 72231; Mature: 72231

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRFSLNKSRQVLHSTYKLLKSKKLAQDPESKQELQKLLEQLEEAIFQQDQEAASQLAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
QAQQFSKRYPASFAKKSWELTKAILFAALAAFLIRQFWFELYEVPTGSMRPTILEQDRMI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEE
VSKTTFGLHFPFKKKPWGFRPEAVTRGGLVVFTVGDLPIPNSDTKYFGFIPGKKRYIKRC
EEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHH
MGKPGDTLYFYGGKIYGLDKEGAVIHFPNDFGLEHLYHVPYISFDGSVEIVNSNKTTAYF
CCCCCCEEEEECCEEEEECCCCCEEECCCCCCCHHHEECCEEEECCEEEEEECCCHHHHH
KQMYQPCGKLSLPQEGPYGQFFHQGVWHNDTPSALKKPHSSPVSYADLFGIGNYAMVRIL
HHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCCCCCHHHHHCCCCEEEEEEE
THKQASLYHTLPNATTQAYLEISHTPNVSYPTPNLQHYNNQIVPTIQPMKTLLPLRQEHI
HHCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHH
HLIRNNLTTSRFVISDGVACKYQPFSTDPEGAAKLYGLPFPGVDDGCYEYSKGEAYKIGF
HHHHHCCCCCEEEEECCCEEEECCCCCCCCCCCEEECCCCCCCCCCHHCCCCCCEEEEEC
GGMRYKLKPTHPLMQLNDNQVIDLFNCGMNFSSFFIPKNPKYNPLPNRYAFYNQGNLYVM
CCEEEEECCCCCCEEECCCCEEEEEECCCCCCEEECCCCCCCCCCCCCEEEEECCCEEEE
DSPIFIKNDPALQKFVESEKTKQEESTEDRPYISFIDRGPPPQDPEQFAEFIRNFGIQIP
CCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCC
ENHVLVLGDNYPMSADSREFGFVPLENLLGSPLWIFWPLGHFGRLKNVPAPTTLPGYLVN
CCCEEEEECCCCCCCCCCCCCCEEHHHHHCCCEEEEECCCHHCCCCCCCCCCCCHHHHHH
GLALGFFVYIFGYIRYQRRHRLFPKNHKKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKDRFSLNKSRQVLHSTYKLLKSKKLAQDPESKQELQKLLEQLEEAIFQQDQEAASQLAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
QAQQFSKRYPASFAKKSWELTKAILFAALAAFLIRQFWFELYEVPTGSMRPTILEQDRMI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEE
VSKTTFGLHFPFKKKPWGFRPEAVTRGGLVVFTVGDLPIPNSDTKYFGFIPGKKRYIKRC
EEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHH
MGKPGDTLYFYGGKIYGLDKEGAVIHFPNDFGLEHLYHVPYISFDGSVEIVNSNKTTAYF
CCCCCCEEEEECCEEEEECCCCCEEECCCCCCCHHHEECCEEEECCEEEEEECCCHHHHH
KQMYQPCGKLSLPQEGPYGQFFHQGVWHNDTPSALKKPHSSPVSYADLFGIGNYAMVRIL
HHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCCCCCHHHHHCCCCEEEEEEE
THKQASLYHTLPNATTQAYLEISHTPNVSYPTPNLQHYNNQIVPTIQPMKTLLPLRQEHI
HHCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHH
HLIRNNLTTSRFVISDGVACKYQPFSTDPEGAAKLYGLPFPGVDDGCYEYSKGEAYKIGF
HHHHHCCCCCEEEEECCCEEEECCCCCCCCCCCEEECCCCCCCCCCHHCCCCCCEEEEEC
GGMRYKLKPTHPLMQLNDNQVIDLFNCGMNFSSFFIPKNPKYNPLPNRYAFYNQGNLYVM
CCEEEEECCCCCCEEECCCCEEEEEECCCCCCEEECCCCCCCCCCCCCEEEEECCCEEEE
DSPIFIKNDPALQKFVESEKTKQEESTEDRPYISFIDRGPPPQDPEQFAEFIRNFGIQIP
CCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCC
ENHVLVLGDNYPMSADSREFGFVPLENLLGSPLWIFWPLGHFGRLKNVPAPTTLPGYLVN
CCCEEEEECCCCCCCCCCCCCCEEHHHHHCCCEEEEECCCHHCCCCCCCCCCCCHHHHHH
GLALGFFVYIFGYIRYQRRHRLFPKNHKKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA