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The map label for this gene is maiA [H]

Identifier: 23501726

GI number: 23501726

Start: 816589

End: 817236

Strand: Reverse

Name: maiA [H]

Synonym: BR0839

Alternate gene names: 23501726

Gene position: 817236-816589 (Counterclockwise)

Preceding gene: 23501727

Following gene: 23501725

Centisome position: 38.77

GC content: 57.25

Gene sequence:

>648_bases
ATGCAGCTTTATTCCCGCCCTCTTTCCCCTTACTCGGCTTTTGTCCGCGGGGTTCTTTACCTGAAAGACCTGCCGTTCAA
GCCGATGACCCTGCCCTATCCGTTGCCGGCCGATTTCGGCACGATCACGCCGCTGCGCCGCGTTCCCGTGCTGATTACCG
GTTCCGGTGAAACGCTCTATGAAGGAGCGGTGATCGCCGAATATCTGGAAGATCATTTTCCCGAGACACCCCTTCTGCCC
GGCACCCCGCGCGAACGCGCGCTTATCCGGCTTCTTGCGCGCGTGAGCGAGCTTGAAGTGCTTGGCCCTGCGATGAAACT
GTTCATCCTTCTCAGCAAGCCCGAACGCGACAATGCAAGTATCGAACGCCTGTTCGACAAGATGCATACCGGCCTGAAAG
TGGTTGAAAGCCGCCTCTCGGACAAGGCCTATGCAACAGGCGACGAACCCACCCTTGCCGATTGCTGGGTGCTGCCGGTG
CGTTTTCTCATGGAACCGCTGAAGAAACTGTCTGACAGAGCGGATTTGCTGGACGAGTTCCCGAAATTCGATGCATATGC
CGCCAAGGCAAAACAGCATCCGGTTTTTGAGCGCATCTGGAACGAGATGAGCGACGGGCTGAAAGCTTTCATGCCGCAAC
TCGCCTGA

Upstream 100 bases:

>100_bases
CCCATATTTGTTTCACGCATTCTCCCGGCATAAAGAGTTCACATTTTTGCTGGAAATGCCTCGCATATTTTGGCGACAAC
GAATCAGGGGAACATCATTC

Downstream 100 bases:

>100_bases
CCCTGTTTACCCCACCTCTCATGCGTGCCTTTGAAAGGCGCGCACTATCGGAGCCTTCCTTGAAGACCAAGACTTTCGCT
CTTCTGGCCCTCCCGGCCAT

Product: glutathione S-transferase family protein

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MQLYSRPLSPYSAFVRGVLYLKDLPFKPMTLPYPLPADFGTITPLRRVPVLITGSGETLYEGAVIAEYLEDHFPETPLLP
GTPRERALIRLLARVSELEVLGPAMKLFILLSKPERDNASIERLFDKMHTGLKVVESRLSDKAYATGDEPTLADCWVLPV
RFLMEPLKKLSDRADLLDEFPKFDAYAAKAKQHPVFERIWNEMSDGLKAFMPQLA

Sequences:

>Translated_215_residues
MQLYSRPLSPYSAFVRGVLYLKDLPFKPMTLPYPLPADFGTITPLRRVPVLITGSGETLYEGAVIAEYLEDHFPETPLLP
GTPRERALIRLLARVSELEVLGPAMKLFILLSKPERDNASIERLFDKMHTGLKVVESRLSDKAYATGDEPTLADCWVLPV
RFLMEPLKKLSDRADLLDEFPKFDAYAAKAKQHPVFERIWNEMSDGLKAFMPQLA
>Mature_215_residues
MQLYSRPLSPYSAFVRGVLYLKDLPFKPMTLPYPLPADFGTITPLRRVPVLITGSGETLYEGAVIAEYLEDHFPETPLLP
GTPRERALIRLLARVSELEVLGPAMKLFILLSKPERDNASIERLFDKMHTGLKVVESRLSDKAYATGDEPTLADCWVLPV
RFLMEPLKKLSDRADLLDEFPKFDAYAAKAKQHPVFERIWNEMSDGLKAFMPQLA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24350; Mature: 24350

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYSRPLSPYSAFVRGVLYLKDLPFKPMTLPYPLPADFGTITPLRRVPVLITGSGETLY
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCCCHHH
EGAVIAEYLEDHFPETPLLPGTPRERALIRLLARVSELEVLGPAMKLFILLSKPERDNAS
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
IERLFDKMHTGLKVVESRLSDKAYATGDEPTLADCWVLPVRFLMEPLKKLSDRADLLDEF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKFDAYAAKAKQHPVFERIWNEMSDGLKAFMPQLA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLYSRPLSPYSAFVRGVLYLKDLPFKPMTLPYPLPADFGTITPLRRVPVLITGSGETLY
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCCCHHH
EGAVIAEYLEDHFPETPLLPGTPRERALIRLLARVSELEVLGPAMKLFILLSKPERDNAS
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
IERLFDKMHTGLKVVESRLSDKAYATGDEPTLADCWVLPVRFLMEPLKKLSDRADLLDEF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKFDAYAAKAKQHPVFERIWNEMSDGLKAFMPQLA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]