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The map label for this gene is purN [H]

Identifier: 161486698

GI number: 161486698

Start: 695970

End: 696587

Strand: Reverse

Name: purN [H]

Synonym: BR0709

Alternate gene names: 161486698

Gene position: 696587-695970 (Counterclockwise)

Preceding gene: 23501597

Following gene: 23501595

Centisome position: 33.05

GC content: 59.71

Gene sequence:

>618_bases
ATGAAACGCAACCGGGTCGTCATCTTTATTTCAGGCGACGGCTCCAACATGGAAGCGCTGATCCGCGCGGCACAAGCGCC
CGGTTTTCCGGCGGAAATCGTAGCGGTCTTTTCCGACAAGGCAGAAGCGGGCGGCCTTGCCAAGGCAGAAGCGGCAGGCA
TTGCCACGCAGGTTTTCAAGCGCAAGGACTTTGCTTCCAAAGAAGCGCATGAGGATGCGATCCTTGCCGCTCTCGACGTT
CTCAAGCCGGATATTATCTGCCTTGCAGGCTATATGCGCCTTCTGTCAGGCCGCTTTATTGCCCCTTACGAGGGGCGCAT
TCTTAATATCCACCCTTCCCTGCTACCGCTTTTTCCGGGCCTGCACACGCACCAGCGCGCGCTCGATGCGGGCATGAAGC
TTGCGGGCTGCACCGTCCATCTGGTGACGGAAGGCATGGACGAAGGGCCAATCCTCGCACAAGCCGCAGTGCCGGTGCTG
GACGGCGATACAGCCGAAACGCTGGCCGCGCGCGTGCTGAAGGCCGAACACCGGCTTTATCCGCTTGCGCTTCAAAAGTT
TGCGGCTGGAGAGAAGGCTTCCAACCAGTTTTCGGACGGCATGGTGCTCAGCGCCTGA

Upstream 100 bases:

>100_bases
TGATCGCAGCACTTGCAGCCGAAGGCGAAAAGGTCGTTACACTCGGCCGCATGACCCGGCGTGAGAAAGACGGCGTCATC
TACAAGGGCCAGCTCGCACT

Downstream 100 bases:

>100_bases
AAGGCTTTCCGGCCCGACGCGACAAATTGATCACACATACAATTTTTCGTTAACAAAACTTTAATATCCCGGAAAAAGCT
TTATTTCCGCTGCTTAAGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKRNRVVIFISGDGSNMEALIRAAQAPGFPAEIVAVFSDKAEAGGLAKAEAAGIATQVFKRKDFASKEAHEDAILAALDV
LKPDIICLAGYMRLLSGRFIAPYEGRILNIHPSLLPLFPGLHTHQRALDAGMKLAGCTVHLVTEGMDEGPILAQAAVPVL
DGDTAETLAARVLKAEHRLYPLALQKFAAGEKASNQFSDGMVLSA

Sequences:

>Translated_205_residues
MKRNRVVIFISGDGSNMEALIRAAQAPGFPAEIVAVFSDKAEAGGLAKAEAAGIATQVFKRKDFASKEAHEDAILAALDV
LKPDIICLAGYMRLLSGRFIAPYEGRILNIHPSLLPLFPGLHTHQRALDAGMKLAGCTVHLVTEGMDEGPILAQAAVPVL
DGDTAETLAARVLKAEHRLYPLALQKFAAGEKASNQFSDGMVLSA
>Mature_205_residues
MKRNRVVIFISGDGSNMEALIRAAQAPGFPAEIVAVFSDKAEAGGLAKAEAAGIATQVFKRKDFASKEAHEDAILAALDV
LKPDIICLAGYMRLLSGRFIAPYEGRILNIHPSLLPLFPGLHTHQRALDAGMKLAGCTVHLVTEGMDEGPILAQAAVPVL
DGDTAETLAARVLKAEHRLYPLALQKFAAGEKASNQFSDGMVLSA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=48.6772486772487, Blast_Score=184, Evalue=4e-47,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=48.6772486772487, Blast_Score=184, Evalue=4e-47,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=48.6772486772487, Blast_Score=184, Evalue=4e-47,
Organism=Escherichia coli, GI1788846, Length=186, Percent_Identity=46.7741935483871, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787483, Length=187, Percent_Identity=31.5508021390374, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=42.5531914893617, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=35.8585858585859, Blast_Score=82, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=48.1675392670157, Blast_Score=183, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21743; Mature: 21743

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNRVVIFISGDGSNMEALIRAAQAPGFPAEIVAVFSDKAEAGGLAKAEAAGIATQVFK
CCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHH
RKDFASKEAHEDAILAALDVLKPDIICLAGYMRLLSGRFIAPYEGRILNIHPSLLPLFPG
HHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCEEEEECCHHHHHCCC
LHTHQRALDAGMKLAGCTVHLVTEGMDEGPILAQAAVPVLDGDTAETLAARVLKAEHRLY
CCHHHHHHHHCCEECCCEEEEEECCCCCCCEEHHHCCEEECCCHHHHHHHHHHHHHHHHH
PLALQKFAAGEKASNQFSDGMVLSA
HHHHHHHHCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MKRNRVVIFISGDGSNMEALIRAAQAPGFPAEIVAVFSDKAEAGGLAKAEAAGIATQVFK
CCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHH
RKDFASKEAHEDAILAALDVLKPDIICLAGYMRLLSGRFIAPYEGRILNIHPSLLPLFPG
HHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCEEEEECCHHHHHCCC
LHTHQRALDAGMKLAGCTVHLVTEGMDEGPILAQAAVPVLDGDTAETLAARVLKAEHRLY
CCHHHHHHHHCCEECCCEEEEEECCCCCCCEEHHHCCEEECCCHHHHHHHHHHHHHHHHH
PLALQKFAAGEKASNQFSDGMVLSA
HHHHHHHHCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]