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The map label for this gene is pbpE [H]

Identifier: 23100815

GI number: 23100815

Start: 3495151

End: 3496512

Strand: Reverse

Name: pbpE [H]

Synonym: OB3360

Alternate gene names: 23100815

Gene position: 3496512-3495151 (Counterclockwise)

Preceding gene: 23100816

Following gene: 23100814

Centisome position: 96.31

GC content: 42.29

Gene sequence:

>1362_bases
TTGGATAAAATAAAGTACGAAGAGTTTGCGGAATCGCTTATAAAAGCTCATCAAACCCCAGGAGCAATAGTCGCATTAAA
CCATCAACGGTATGAAAAAGGGTTCGGTTACCGAGATCGAGAAAATGAATTACCTGTTACAGCGGACACTATTTTTGGAA
TTGGAAGTATTACGAAGTCTATGACCTGCATCGCCATACTTCAACTGCAAGAAGCCGGCAAACTATCTGTCCACGACACC
ATTAAAACATATCTTCCTGAGATTACGTTTAGAGGGAGTGAAGATATAACACTTCACCATCTAATGACACATACAGCCGG
CATTCCCCCGTTAAACACACTTTTTTACGCGAATAAGCAATCAATGGAGGAAGATGGTTCCTTCGATTTTCAAATTAAGC
TCGGTGTCCCGCTTGATCCTGGACAAGATAGCATTGATACGTACGATGATCTTATAAACTATCTAAATACGATTCAAATT
GACCCGATTGACCGTCCTGGGCGTTCATTCAGCTATTCAAATGATGGCTACGCTCTTCTTGGCATTATTATTGAACGTGT
TAGCGGGCAGTCGTATGCACACTATATAGAGGCTAATATCTTCCAGCCACTTGGTATGAAAAGTAGTTTTTTTGATCCAG
AGCGATTGAATAATCGAGCAACGAGTCTGTATAGCGTTAACCCGCTTGCTGAGACACCAGCAGTGGAACACTCGCCTGGT
TGGTGGGATGCGCCTGCGATGTGGGCAGCCGGTTATGTAAAGTCAACTGCTGCTGATATGCTTCGGTTTGGAGATGTCTT
TTTGAATGAGGGCAATGGTATTTTAACTCCGGAAAGCCTTGATGACTTAACTTCTCAGCATATTGAAGTAGAGCCAGGCC
TATATTATGGTTACGGTGTCATCATTAACCCTGATTTTTTTGGCACAACACTCATTGAACATGGTGGAGGAATTAAAGGT
GTGTCAGCGCAGTTTTCCGTATTGCCTGAGAAGGATATTGTTGGTGTTTGTTTGACGAACATTGCTGGTGCTCCTGCTGA
AGCGCTGTTAAAAGGGGCAATACAAAGCGAGATGGATATTGAGCCTGATAAAAAAGCAATCGAGTATGACGATTATATGC
TCTCTGAAGCAGATCGCCAAGCAATTATCGGTACGTATCATTCTAATGAGGGACAAACGATCAAAGTCTATGAAAAGGAT
GAAGCTATTTTTATAGATTATTTAGGAACGGAAATTCCAACCCGCCCTGTGGGGAAAAGTGATGTTACCATAGCGATGCA
TGGGACTGAGCACTTATTGCAGTTTAAAGACGACCGTCTTCATTTTGGGTTTAGACAACTGTTACGAATGGATAGTGAAT
AG

Upstream 100 bases:

>100_bases
TTAGAGCCTAACAACTATAGGGTTTATCTTCATAATTTTCTTGAAACTATTCGACTATTAAAACGTATAATTAGTTAACT
TAATAGGGAGGGATCTAACC

Downstream 100 bases:

>100_bases
CGTTGAAGGATCTCTTTTATTAATTTACTTATCTAAATATCTTTATATAGTACAATTTAAAACATGTAAATTTGATTAAA
GAGGTGAAGCATCATGTCAA

Product: hypothetical protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MDKIKYEEFAESLIKAHQTPGAIVALNHQRYEKGFGYRDRENELPVTADTIFGIGSITKSMTCIAILQLQEAGKLSVHDT
IKTYLPEITFRGSEDITLHHLMTHTAGIPPLNTLFYANKQSMEEDGSFDFQIKLGVPLDPGQDSIDTYDDLINYLNTIQI
DPIDRPGRSFSYSNDGYALLGIIIERVSGQSYAHYIEANIFQPLGMKSSFFDPERLNNRATSLYSVNPLAETPAVEHSPG
WWDAPAMWAAGYVKSTAADMLRFGDVFLNEGNGILTPESLDDLTSQHIEVEPGLYYGYGVIINPDFFGTTLIEHGGGIKG
VSAQFSVLPEKDIVGVCLTNIAGAPAEALLKGAIQSEMDIEPDKKAIEYDDYMLSEADRQAIIGTYHSNEGQTIKVYEKD
EAIFIDYLGTEIPTRPVGKSDVTIAMHGTEHLLQFKDDRLHFGFRQLLRMDSE

Sequences:

>Translated_453_residues
MDKIKYEEFAESLIKAHQTPGAIVALNHQRYEKGFGYRDRENELPVTADTIFGIGSITKSMTCIAILQLQEAGKLSVHDT
IKTYLPEITFRGSEDITLHHLMTHTAGIPPLNTLFYANKQSMEEDGSFDFQIKLGVPLDPGQDSIDTYDDLINYLNTIQI
DPIDRPGRSFSYSNDGYALLGIIIERVSGQSYAHYIEANIFQPLGMKSSFFDPERLNNRATSLYSVNPLAETPAVEHSPG
WWDAPAMWAAGYVKSTAADMLRFGDVFLNEGNGILTPESLDDLTSQHIEVEPGLYYGYGVIINPDFFGTTLIEHGGGIKG
VSAQFSVLPEKDIVGVCLTNIAGAPAEALLKGAIQSEMDIEPDKKAIEYDDYMLSEADRQAIIGTYHSNEGQTIKVYEKD
EAIFIDYLGTEIPTRPVGKSDVTIAMHGTEHLLQFKDDRLHFGFRQLLRMDSE
>Mature_453_residues
MDKIKYEEFAESLIKAHQTPGAIVALNHQRYEKGFGYRDRENELPVTADTIFGIGSITKSMTCIAILQLQEAGKLSVHDT
IKTYLPEITFRGSEDITLHHLMTHTAGIPPLNTLFYANKQSMEEDGSFDFQIKLGVPLDPGQDSIDTYDDLINYLNTIQI
DPIDRPGRSFSYSNDGYALLGIIIERVSGQSYAHYIEANIFQPLGMKSSFFDPERLNNRATSLYSVNPLAETPAVEHSPG
WWDAPAMWAAGYVKSTAADMLRFGDVFLNEGNGILTPESLDDLTSQHIEVEPGLYYGYGVIINPDFFGTTLIEHGGGIKG
VSAQFSVLPEKDIVGVCLTNIAGAPAEALLKGAIQSEMDIEPDKKAIEYDDYMLSEADRQAIIGTYHSNEGQTIKVYEKD
EAIFIDYLGTEIPTRPVGKSDVTIAMHGTEHLLQFKDDRLHFGFRQLLRMDSE

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=255, Percent_Identity=29.8039215686275, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533026, Length=381, Percent_Identity=25.7217847769029, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 50383; Mature: 50383

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIKYEEFAESLIKAHQTPGAIVALNHQRYEKGFGYRDRENELPVTADTIFGIGSITKS
CCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHH
MTCIAILQLQEAGKLSVHDTIKTYLPEITFRGSEDITLHHLMTHTAGIPPLNTLFYANKQ
HHHEEEEEHHCCCCCCHHHHHHHHCCCEEECCCCCEEHHHHHHHHCCCCCCHHEEECCCC
SMEEDGSFDFQIKLGVPLDPGQDSIDTYDDLINYLNTIQIDPIDRPGRSFSYSNDGYALL
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHH
GIIIERVSGQSYAHYIEANIFQPLGMKSSFFDPERLNNRATSLYSVNPLAETPAVEHSPG
HHHHHHHCCCCEEEEEEHHCCCCCCCCCCCCCHHHHCCHHCEEEEECCCCCCCCCCCCCC
WWDAPAMWAAGYVKSTAADMLRFGDVFLNEGNGILTPESLDDLTSQHIEVEPGLYYGYGV
CCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEECCCHHHHHHHCCEEECCCEEEEEEE
IINPDFFGTTLIEHGGGIKGVSAQFSVLPEKDIVGVCLTNIAGAPAEALLKGAIQSEMDI
EECCCCCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
EPDKKAIEYDDYMLSEADRQAIIGTYHSNEGQTIKVYEKDEAIFIDYLGTEIPTRPVGKS
CCCCCCEECCHHHHCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCC
DVTIAMHGTEHLLQFKDDRLHFGFRQLLRMDSE
CEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKIKYEEFAESLIKAHQTPGAIVALNHQRYEKGFGYRDRENELPVTADTIFGIGSITKS
CCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHH
MTCIAILQLQEAGKLSVHDTIKTYLPEITFRGSEDITLHHLMTHTAGIPPLNTLFYANKQ
HHHEEEEEHHCCCCCCHHHHHHHHCCCEEECCCCCEEHHHHHHHHCCCCCCHHEEECCCC
SMEEDGSFDFQIKLGVPLDPGQDSIDTYDDLINYLNTIQIDPIDRPGRSFSYSNDGYALL
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHH
GIIIERVSGQSYAHYIEANIFQPLGMKSSFFDPERLNNRATSLYSVNPLAETPAVEHSPG
HHHHHHHCCCCEEEEEEHHCCCCCCCCCCCCCHHHHCCHHCEEEEECCCCCCCCCCCCCC
WWDAPAMWAAGYVKSTAADMLRFGDVFLNEGNGILTPESLDDLTSQHIEVEPGLYYGYGV
CCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEECCCHHHHHHHCCEEECCCEEEEEEE
IINPDFFGTTLIEHGGGIKGVSAQFSVLPEKDIVGVCLTNIAGAPAEALLKGAIQSEMDI
EECCCCCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
EPDKKAIEYDDYMLSEADRQAIIGTYHSNEGQTIKVYEKDEAIFIDYLGTEIPTRPVGKS
CCCCCCEECCHHHHCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCC
DVTIAMHGTEHLLQFKDDRLHFGFRQLLRMDSE
CEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]