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The map label for this gene is parE [H]

Identifier: 23100693

GI number: 23100693

Start: 3375430

End: 3376410

Strand: Reverse

Name: parE [H]

Synonym: OB3238

Alternate gene names: 23100693

Gene position: 3376410-3375430 (Counterclockwise)

Preceding gene: 23100694

Following gene: 23100692

Centisome position: 93.0

GC content: 37.31

Gene sequence:

>981_bases
GTGAATGGGCTTTATTTATCAAGTCCTGGAGTTATTGAATTTAGAGAGTTAGCTTCCAGGTCAAAATTAAGCGATGATGA
AGTTAGAATTAGGCTAATATACGGAGGGATATGTGGATCTGATATTAGTGTATTTAAAGGAAAGCTTCCACATGCGAACT
ATCCAGTTGTACCTGGTCATGAATTAATAGGAACCGTCATAGAAACAGGTAAATCTGCAGCCGATTTTGCTGGAAAACGC
GTCGCAATTATGCCAAACTCCTTCTGTGGTAAGTGCGAGTATTGTAAGGTAGGAAAAACAAATATTTGTACGGAGAAACA
ATCATTAGGTATTAATAGAGATGGTGGTTTTGCTGAAGAGTTTGTTATCTCGGCAAAATACGTATTGTCATTACCCGATC
AATTGACCAATGAAAAGGCAGTTCTTATTGAACCATTGGCAGTTATTGTTCACGCAATGAAAAAAGTAGTGATTACAGAG
GAAACAAAAGTTGCCATCATCGGATGTGGTAACGAGGGGATGTTAGCAATTGCGGTGGCCGAATACTTAGGCGCTCAAGT
TACCGCCATCGATGTTAAACAAAATAAGTTAAACAAAGTATCTCATAGCTATCACAACGTCTCTACATGTCAATCAGCCG
ATGCTGAGGAATTGTTTGATGTAGTTATAGAAGCAGCAGGAACAAGTGAATCCTTTGAACAGGGAATACAATTATTAAAA
CCAGGTGGAGCCTTTGTAGCTATAGGAATGCCGCCACATGCAAATTTACCAGTTGTAGAAATCGTTCGCAAGGAAGTAAC
AATTTATGGATCAATAATATATAACTTCCCAGAAGACTTTAATACGAGTATAGAATTCTTGTTACAGGAAGATTTTCAAG
TAGAACCAGTTATTTCAAAAATTCTTTCTGTAAACGAATATGAAAAGGCATACACGTATGCGGTATCAGGAGATTATGGG
AAAATTATTTTGAAATTCTAG

Upstream 100 bases:

>100_bases
AGAAGGCCCTCGATATGCTCTTGAAGAAATGACAGAATCAAAAATGGTAGTGCTGCGCGTGTAATAGAAAAGGTAATTTG
AGGAATAGAAGGTGAATAAT

Downstream 100 bases:

>100_bases
GAGGTAATGTACATGAAAAGACTAGTTTCTAATTTGTTAGTTGAATATTTGGAAGATAGAGGTGTGGAACATATATTTGG
ATTGTGTGGTCACACCAACA

Product: sorbitol dehydrogenase

Products: L-sorbose; NADH; H+

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNGLYLSSPGVIEFRELASRSKLSDDEVRIRLIYGGICGSDISVFKGKLPHANYPVVPGHELIGTVIETGKSAADFAGKR
VAIMPNSFCGKCEYCKVGKTNICTEKQSLGINRDGGFAEEFVISAKYVLSLPDQLTNEKAVLIEPLAVIVHAMKKVVITE
ETKVAIIGCGNEGMLAIAVAEYLGAQVTAIDVKQNKLNKVSHSYHNVSTCQSADAEELFDVVIEAAGTSESFEQGIQLLK
PGGAFVAIGMPPHANLPVVEIVRKEVTIYGSIIYNFPEDFNTSIEFLLQEDFQVEPVISKILSVNEYEKAYTYAVSGDYG
KIILKF

Sequences:

>Translated_326_residues
MNGLYLSSPGVIEFRELASRSKLSDDEVRIRLIYGGICGSDISVFKGKLPHANYPVVPGHELIGTVIETGKSAADFAGKR
VAIMPNSFCGKCEYCKVGKTNICTEKQSLGINRDGGFAEEFVISAKYVLSLPDQLTNEKAVLIEPLAVIVHAMKKVVITE
ETKVAIIGCGNEGMLAIAVAEYLGAQVTAIDVKQNKLNKVSHSYHNVSTCQSADAEELFDVVIEAAGTSESFEQGIQLLK
PGGAFVAIGMPPHANLPVVEIVRKEVTIYGSIIYNFPEDFNTSIEFLLQEDFQVEPVISKILSVNEYEKAYTYAVSGDYG
KIILKF
>Mature_326_residues
MNGLYLSSPGVIEFRELASRSKLSDDEVRIRLIYGGICGSDISVFKGKLPHANYPVVPGHELIGTVIETGKSAADFAGKR
VAIMPNSFCGKCEYCKVGKTNICTEKQSLGINRDGGFAEEFVISAKYVLSLPDQLTNEKAVLIEPLAVIVHAMKKVVITE
ETKVAIIGCGNEGMLAIAVAEYLGAQVTAIDVKQNKLNKVSHSYHNVSTCQSADAEELFDVVIEAAGTSESFEQGIQLLK
PGGAFVAIGMPPHANLPVVEIVRKEVTIYGSIIYNFPEDFNTSIEFLLQEDFQVEPVISKILSVNEYEKAYTYAVSGDYG
KIILKF

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=324, Percent_Identity=28.0864197530864, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788407, Length=322, Percent_Identity=31.6770186335404, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790045, Length=326, Percent_Identity=27.9141104294479, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI226510992, Length=268, Percent_Identity=32.8358208955224, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788073, Length=310, Percent_Identity=27.0967741935484, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787863, Length=260, Percent_Identity=30.3846153846154, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790718, Length=311, Percent_Identity=27.9742765273312, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788075, Length=335, Percent_Identity=23.8805970149254, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082125, Length=352, Percent_Identity=25, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786825, Length=254, Percent_Identity=25.1968503937008, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081918, Length=290, Percent_Identity=27.2413793103448, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082401, Length=270, Percent_Identity=28.1481481481481, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786518, Length=327, Percent_Identity=25.0764525993884, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=344, Percent_Identity=27.906976744186, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17562878, Length=340, Percent_Identity=25.2941176470588, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562584, Length=301, Percent_Identity=24.5847176079734, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=316, Percent_Identity=23.7341772151899, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562582, Length=307, Percent_Identity=26.7100977198697, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322619, Length=313, Percent_Identity=28.7539936102236, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319955, Length=313, Percent_Identity=28.7539936102236, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323099, Length=332, Percent_Identity=30.421686746988, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319621, Length=307, Percent_Identity=25.4071661237785, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319258, Length=355, Percent_Identity=23.6619718309859, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=332, Percent_Identity=25.3012048192771, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=326, Percent_Identity=24.8466257668712, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=310, Percent_Identity=24.5161290322581, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=342, Percent_Identity=24.5614035087719, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=202, Percent_Identity=27.7227722772277, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737897, Length=321, Percent_Identity=27.4143302180685, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137530, Length=333, Percent_Identity=28.2282282282282, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.14

Molecular weight: Translated: 35440; Mature: 35440

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLYLSSPGVIEFRELASRSKLSDDEVRIRLIYGGICGSDISVFKGKLPHANYPVVPGH
CCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCH
ELIGTVIETGKSAADFAGKRVAIMPNSFCGKCEYCKVGKTNICTEKQSLGINRDGGFAEE
HHHHHHHHCCCCHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCCHHHCCCCCCCCCHHH
FVISAKYVLSLPDQLTNEKAVLIEPLAVIVHAMKKVVITEETKVAIIGCGNEGMLAIAVA
HHHHHHHEEECCHHHCCCCEEEEHHHHHHHHHHHHHEEECCCEEEEEEECCCCEEEEEEH
EYLGAQVTAIDVKQNKLNKVSHSYHNVSTCQSADAEELFDVVIEAAGTSESFEQGIQLLK
HHHCCEEEEEECCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHEEC
PGGAFVAIGMPPHANLPVVEIVRKEVTIYGSIIYNFPEDFNTSIEFLLQEDFQVEPVISK
CCCCEEEEECCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHH
ILSVNEYEKAYTYAVSGDYGKIILKF
HHCCCCCHHEEEEEEECCCCEEEEEC
>Mature Secondary Structure
MNGLYLSSPGVIEFRELASRSKLSDDEVRIRLIYGGICGSDISVFKGKLPHANYPVVPGH
CCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCH
ELIGTVIETGKSAADFAGKRVAIMPNSFCGKCEYCKVGKTNICTEKQSLGINRDGGFAEE
HHHHHHHHCCCCHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCCHHHCCCCCCCCCHHH
FVISAKYVLSLPDQLTNEKAVLIEPLAVIVHAMKKVVITEETKVAIIGCGNEGMLAIAVA
HHHHHHHEEECCHHHCCCCEEEEHHHHHHHHHHHHHEEECCCEEEEEEECCCCEEEEEEH
EYLGAQVTAIDVKQNKLNKVSHSYHNVSTCQSADAEELFDVVIEAAGTSESFEQGIQLLK
HHHCCEEEEEECCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHEEC
PGGAFVAIGMPPHANLPVVEIVRKEVTIYGSIIYNFPEDFNTSIEFLLQEDFQVEPVISK
CCCCEEEEECCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHH
ILSVNEYEKAYTYAVSGDYGKIILKF
HHCCCCCHHEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-iditol; NAD+

Specific reaction: L-iditol + NAD+ = L-sorbose + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA