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The map label for this gene is ilvD

Identifier: 23100079

GI number: 23100079

Start: 2689070

End: 2690755

Strand: Reverse

Name: ilvD

Synonym: OB2624

Alternate gene names: 23100079

Gene position: 2690755-2689070 (Counterclockwise)

Preceding gene: 23100082

Following gene: 23100078

Centisome position: 74.11

GC content: 40.63

Gene sequence:

>1686_bases
ATGGGAAAAGATTTGCGGATAAAAAGTAAAGCATTTGACGGTACTATGCGAGCTCCAAATAGGGCAATGCTTCGTGCGGT
CGGTCTGACAGATGAAGATTTTAAGAAGCCTATGATTGGTGTAGCAAGTACATGGGCAGAAGTAACACCATGTAATATAC
ATTTAAATGATTTAGCTCTTCTTGCCAAAAAAGGGGTCCGGCACTCGGATGCTGTTCCTTTGGTCTTTAATACGATAACC
GTATCTGATGGAATTTCCATGGGAACACAAGGTATGAACTACTCATTGCCGAGTCGAGATCTTATTGCTGATTCAATCGA
AACCGTTGTTGGAGCAGAAAATTTAGATGGATTAGTAGCAATTGGAGCCTGTGACAAGAATATTCCAGGGTGCTTGATTG
CGATTGCTAATGCAGGAGTTCCCTCGGTATTTGTATATGGCGGAACAATTGCCCCAGGAAATGTGGATGGGAAAGATATA
GATATTGTTTCAGTTTTCGAAGGAGTAGGACAACATAATGCAGGAGCTATTGACGATAATCAATTAAGAAAAGTAGAATG
TAATGCTTGTCCAGGTGCAGGAGCATGTGGTGGAATGTATACCGCCAATACAATGGCCTCTGCAGCGGAAGCTTTAGGAA
TCAGTTTACCGGGAAGCTCTTCTAACCCAGCTGAATCAGAAGAAAAGCAGGGTGATGTGGAGGCAGCTGGTGAAGCGATT
AAAGATTTATTAGAGAAAGGTATTTATCCAAAAGATATTCTAACGAAAAAAGCTTTTGAAAATGCAATTACAGTAGTTAT
GGCATTGGGAGGATCTACAAATGCGATCTTGCATTTACTAGCAGTTTCTCATGCCGCAGAAGTAGACTTAACGATTGATG
ATTTTAATCGAATCCAGAAAAAAGTACCGCATTTAGCCGATTTAAAACCAAGTGGACGTTTTGTAATGCAAGACCTTCAT
CGAGTTGGTGGGGTACAAGCAGTAATGAAATTATTGCATGAAAATGGATACTTACATGGCGACTGTCTTACTGTAACAGG
AAAGACTATAGCAGAAAATCTAGCTGAAGCTCCTGCTTTGAAAGAAAATCAACAAGTCATTATGCCGTTTGATAATCCAA
AAAGGGAAGATGGTCCTCTGATTGTCTTAAAGGGGAATTTATCACCAACTGGTGCTGTGGCAAAAGTATCAGGGGTGAAA
GTGAAACGACACACAGGTCCTGCACGTGTATTCAACACAGAAAAAGAGGCAACTCAAGCAATTCTGGATAACAAAATTAA
AGAGGGAGATGTATTAGTTATTCGTTATGTTGGACCAAAAGGTGGTCCCGGAATGCCGGAGATGTTGTCTGTTTCTAGTA
TTCTTGTAGGTAAAGGAATGGGAGAATCGGTAGCTTTATTGACGGATGGTAGATTCTCTGGTGGTACACATGGTCTTGTG
GTAGGTCATATAAGTCCAGAGGCACAAGATGGCGGACCAATTGCATTTCTGCAAGAAGGAGATATGGTGACCATAGATTC
TAATAAAAGAGAAATTTCCATGGACGTTTCGGAAGATGAAATAAAACTACGTCAAGGAAAATGGGAAGCCCCTGAACTAC
ATAAGAAAGGGGTTCTTGGAAAATATGCACATAATGTTACTTGCTCATCCAAAGGTGCAGTAACAGATTATTTGAACCGA
GAATAA

Upstream 100 bases:

>100_bases
TAAGGTGCTTGAGTATCAGAAAATAAACTAAAAAGACGAAATTCATTGAACTATAAAAATATTAGTCATATAATAGTCCG
AAAAGAAGGGTGGAATATTT

Downstream 100 bases:

>100_bases
ACCAAAAGCTGTCAGTTAGGTCAAGTCAGACTTAATTGATAGCTTTTTTCTTGTGTTCAAGAATAAACTGATTGTTAAGT
GTTATACAATAATCAATTTA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MGKDLRIKSKAFDGTMRAPNRAMLRAVGLTDEDFKKPMIGVASTWAEVTPCNIHLNDLALLAKKGVRHSDAVPLVFNTIT
VSDGISMGTQGMNYSLPSRDLIADSIETVVGAENLDGLVAIGACDKNIPGCLIAIANAGVPSVFVYGGTIAPGNVDGKDI
DIVSVFEGVGQHNAGAIDDNQLRKVECNACPGAGACGGMYTANTMASAAEALGISLPGSSSNPAESEEKQGDVEAAGEAI
KDLLEKGIYPKDILTKKAFENAITVVMALGGSTNAILHLLAVSHAAEVDLTIDDFNRIQKKVPHLADLKPSGRFVMQDLH
RVGGVQAVMKLLHENGYLHGDCLTVTGKTIAENLAEAPALKENQQVIMPFDNPKREDGPLIVLKGNLSPTGAVAKVSGVK
VKRHTGPARVFNTEKEATQAILDNKIKEGDVLVIRYVGPKGGPGMPEMLSVSSILVGKGMGESVALLTDGRFSGGTHGLV
VGHISPEAQDGGPIAFLQEGDMVTIDSNKREISMDVSEDEIKLRQGKWEAPELHKKGVLGKYAHNVTCSSKGAVTDYLNR
E

Sequences:

>Translated_561_residues
MGKDLRIKSKAFDGTMRAPNRAMLRAVGLTDEDFKKPMIGVASTWAEVTPCNIHLNDLALLAKKGVRHSDAVPLVFNTIT
VSDGISMGTQGMNYSLPSRDLIADSIETVVGAENLDGLVAIGACDKNIPGCLIAIANAGVPSVFVYGGTIAPGNVDGKDI
DIVSVFEGVGQHNAGAIDDNQLRKVECNACPGAGACGGMYTANTMASAAEALGISLPGSSSNPAESEEKQGDVEAAGEAI
KDLLEKGIYPKDILTKKAFENAITVVMALGGSTNAILHLLAVSHAAEVDLTIDDFNRIQKKVPHLADLKPSGRFVMQDLH
RVGGVQAVMKLLHENGYLHGDCLTVTGKTIAENLAEAPALKENQQVIMPFDNPKREDGPLIVLKGNLSPTGAVAKVSGVK
VKRHTGPARVFNTEKEATQAILDNKIKEGDVLVIRYVGPKGGPGMPEMLSVSSILVGKGMGESVALLTDGRFSGGTHGLV
VGHISPEAQDGGPIAFLQEGDMVTIDSNKREISMDVSEDEIKLRQGKWEAPELHKKGVLGKYAHNVTCSSKGAVTDYLNR
E
>Mature_560_residues
GKDLRIKSKAFDGTMRAPNRAMLRAVGLTDEDFKKPMIGVASTWAEVTPCNIHLNDLALLAKKGVRHSDAVPLVFNTITV
SDGISMGTQGMNYSLPSRDLIADSIETVVGAENLDGLVAIGACDKNIPGCLIAIANAGVPSVFVYGGTIAPGNVDGKDID
IVSVFEGVGQHNAGAIDDNQLRKVECNACPGAGACGGMYTANTMASAAEALGISLPGSSSNPAESEEKQGDVEAAGEAIK
DLLEKGIYPKDILTKKAFENAITVVMALGGSTNAILHLLAVSHAAEVDLTIDDFNRIQKKVPHLADLKPSGRFVMQDLHR
VGGVQAVMKLLHENGYLHGDCLTVTGKTIAENLAEAPALKENQQVIMPFDNPKREDGPLIVLKGNLSPTGAVAKVSGVKV
KRHTGPARVFNTEKEATQAILDNKIKEGDVLVIRYVGPKGGPGMPEMLSVSSILVGKGMGESVALLTDGRFSGGTHGLVV
GHISPEAQDGGPIAFLQEGDMVTIDSNKREISMDVSEDEIKLRQGKWEAPELHKKGVLGKYAHNVTCSSKGAVTDYLNRE

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=592, Percent_Identity=38.8513513513513, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI2367371, Length=488, Percent_Identity=34.0163934426229, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786464, Length=492, Percent_Identity=32.520325203252, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1788157, Length=487, Percent_Identity=30.1848049281314, Blast_Score=185, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=546, Percent_Identity=50.5494505494505, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_OCEIH (Q8EN63)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693545.1
- ProteinModelPortal:   Q8EN63
- SMR:   Q8EN63
- GeneID:   1017083
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2624
- NMPDR:   fig|221109.1.peg.2623
- HOGENOM:   HBG671001
- OMA:   FENAITY
- ProtClustDB:   PRK00911
- BioCyc:   OIHE221109:OB2624-MONOMER
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59136; Mature: 59005

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKDLRIKSKAFDGTMRAPNRAMLRAVGLTDEDFKKPMIGVASTWAEVTPCNIHLNDLAL
CCCCCEEEHHCCCCCCCCCCHHHEEEECCCCHHHHCCCHHHHHHHHHCCCCEEEHHHHHH
LAKKGVRHSDAVPLVFNTITVSDGISMGTQGMNYSLPSRDLIADSIETVVGAENLDGLVA
HHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
IGACDKNIPGCLIAIANAGVPSVFVYGGTIAPGNVDGKDIDIVSVFEGVGQHNAGAIDDN
EECCCCCCCEEEEEEECCCCCEEEEECCEECCCCCCCCCEEHHHHHHHCCCCCCCCCCCC
QLRKVECNACPGAGACGGMYTANTMASAAEALGISLPGSSSNPAESEEKQGDVEAAGEAI
CEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCHHHCCCHHHHHHHH
KDLLEKGIYPKDILTKKAFENAITVVMALGGSTNAILHLLAVSHAAEVDLTIDDFNRIQK
HHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHH
KVPHLADLKPSGRFVMQDLHRVGGVQAVMKLLHENGYLHGDCLTVTGKTIAENLAEAPAL
HCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEECEEEEECCHHHHHHHHHCCCC
KENQQVIMPFDNPKREDGPLIVLKGNLSPTGAVAKVSGVKVKRHTGPARVFNTEKEATQA
CCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCEEEEECCCCCCEECCHHHHHHH
ILDNKIKEGDVLVIRYVGPKGGPGMPEMLSVSSILVGKGMGESVALLTDGRFSGGTHGLV
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEE
VGHISPEAQDGGPIAFLQEGDMVTIDSNKREISMDVSEDEIKLRQGKWEAPELHKKGVLG
EEECCCCCCCCCCEEEEECCCEEEECCCCEEEEECCCCCHHHEECCCCCCCHHHHCCCCH
KYAHNVTCSSKGAVTDYLNRE
HHHCCEEECCCCCCHHHCCCC
>Mature Secondary Structure 
GKDLRIKSKAFDGTMRAPNRAMLRAVGLTDEDFKKPMIGVASTWAEVTPCNIHLNDLAL
CCCCEEEHHCCCCCCCCCCHHHEEEECCCCHHHHCCCHHHHHHHHHCCCCEEEHHHHHH
LAKKGVRHSDAVPLVFNTITVSDGISMGTQGMNYSLPSRDLIADSIETVVGAENLDGLVA
HHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
IGACDKNIPGCLIAIANAGVPSVFVYGGTIAPGNVDGKDIDIVSVFEGVGQHNAGAIDDN
EECCCCCCCEEEEEEECCCCCEEEEECCEECCCCCCCCCEEHHHHHHHCCCCCCCCCCCC
QLRKVECNACPGAGACGGMYTANTMASAAEALGISLPGSSSNPAESEEKQGDVEAAGEAI
CEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCHHHCCCHHHHHHHH
KDLLEKGIYPKDILTKKAFENAITVVMALGGSTNAILHLLAVSHAAEVDLTIDDFNRIQK
HHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHH
KVPHLADLKPSGRFVMQDLHRVGGVQAVMKLLHENGYLHGDCLTVTGKTIAENLAEAPAL
HCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEECEEEEECCHHHHHHHHHCCCC
KENQQVIMPFDNPKREDGPLIVLKGNLSPTGAVAKVSGVKVKRHTGPARVFNTEKEATQA
CCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCEEEEECCCCCCEECCHHHHHHH
ILDNKIKEGDVLVIRYVGPKGGPGMPEMLSVSSILVGKGMGESVALLTDGRFSGGTHGLV
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEE
VGHISPEAQDGGPIAFLQEGDMVTIDSNKREISMDVSEDEIKLRQGKWEAPELHKKGVLG
EEECCCCCCCCCCEEEEECCCEEEECCCCEEEEECCCCCHHHEECCCCCCCHHHHCCCCH
KYAHNVTCSSKGAVTDYLNRE
HHHCCEEECCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376