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The map label for this gene is topB [H]

Identifier: 23100045

GI number: 23100045

Start: 2647991

End: 2650153

Strand: Reverse

Name: topB [H]

Synonym: OB2590

Alternate gene names: 23100045

Gene position: 2650153-2647991 (Counterclockwise)

Preceding gene: 23100047

Following gene: 23100044

Centisome position: 73.0

GC content: 34.67

Gene sequence:

>2163_bases
ATGAGTAAAACAGTAGTCTTAGCAGAAAAGCCATCCGTAGGTCGAGATATTGCAAAAGTGCTCGGATGCCATAAAAAAGG
AAATGGATTTTTAGAAGGATCAAAGTATATTGTAACCTGGGCTTTAGGGCATTTAGTTACATTGGCAGATCCAGATATGT
ATGATAGTAAATATAAAACATGGCGAATGGAAGATTTACCAATGCTTCCAGACCATTTGAAGCTTGTGGTTATGAAAAAG
ACTGGGAAGCAATTTCAGTCGGTAAAAACTCAACTAAACCGTCAAGATGTTGTAGAAATTGTTATTGCTACAGATGCTGG
TAGAGAAGGGGAATTAGTAGCTCGGTGGATTATTGAAAAAGCAAGAGTGAAAAAGCCAATTAAGCGTCTTTGGATTAGTT
CGGTGACAGAGAAAGCAATAAAAGATGGATTTAAACAATTAAAGCCTGGAAAGAATTATTGGAATTTATATCAATCCGCT
GTAGCACGCTCGGAAGCCGATTGGTATGTCGGACTTAATGCTACACGAGCATTAACAACGAAACATAATGCTCAACTTTC
TAGTGGTCGTGTGCAAACACCTACACTGGAAATGGTATCGTTAAGAGAACAAGAAATCAAAAATTTCCAACCGAAAAAAT
TCTATCAATTAGAAGCAAAAACAACCAAAGGTCATCGTTTTCTATGGAAGAATGATGAAAACCAAAACCGCATCTTTTCT
AAAAATGAGGCAATTAAGTTAGAAGAAAAATTAAGTAATAAACAAGCTACGGTAGTCCATGTAGATAAAAAACTAAAAAA
GAAACATGCTCAAGGATTATATGATTTAACGGATTTACAACGGGATGCGAACCGTATATTCCAATTTTCGGGTAAGCAGA
CATTGTCCGTAATGCAGAAGCTATATGAACAGCATAAACTAGTAACGTATCCTCGCACAGATTCAAGGCATTTATCTACT
GATATTGTATCTACTTTAAAGGATCGTGTGAAAGCATGTGGTGTAGATGAATATGCAAAAATAGCAAATACCATTTTAAA
TAAAGAATTACGATTGAATAAATCAGTTGTAGACAATGCAAAAGTAACAGATCATCATGCGATTATTCCAACGGAAGTTC
CAGTTGTTTGGACTGCATTAGATGATAAAGAAAAAAAGATCTATCATTTAATTGTACAAAGATTTCTGGCAGCTTTGTCA
GATCCTATGGAATATGAGCAGACAACAATTACGATTGATATTGCAGGCGAACAATTTATAGCGAACGGCAAAGTAACGAA
AAGACTCGGATGGAAAGAAATTTATAAACATAACGATGAAGAAGATGAAATACAACCATTAAACGTTACTAAAGGAAGTA
AACTAACATTAAAAGTTGAATTAAAAGAGGGAGAAACAAAGCCACCGGAACGTTTTACAGAAGGTACCATTTTGCAAGCG
ATGGAAAATCCAACTCGCTATATGGGTACGAATAAAAAAGAATTGGTAGATACGATTAAAGAAACGGGTGGTATAGGTAC
CGTAGCAACTCGTGCAGATATTATTGAGAAACTATTTATGAATAGCTATATAGAATTAAAAGGAAGAAATATTTATTTAA
CACAAACGGGTCGCCAACTTCTAGATCTCGCACCAGAAGAATTGCGTTCTCCTTTGTTAACTGCAGAATGGGAACAAAAA
TTATCTCTGATTGCGGAAGGTAAATTGAAGAAAGATCAATTTATAAAAGAAATTAAATCATATACAAAAGAAGCAGTTAC
AGAAATTAAGCAAAGTAGTGAAACGTTCAAACATGATAATATGACTGGAACAAAATGTCCAAAGTGCGGAAAACTTATGT
TAGAAATTAAAAATAAACACGGAAAGAAATTGGTATGCCAGGACCGCTCATGTGGAGGTAAGAAAAATATTGCCAAGTTT
ACGAATGCACGCTGTCCAAACTGTCATAAACGATTAGAACTTCGTGGAGAAGGTGAAGGACAAATATTCACTTGTAAGTG
TGGACATCGTGAAAAATTATCTACATTCCAAGCTAGAAAAGAGAAAGAGAAAAAACAACGTGTGTCAAAGAAAGATGTGA
AGAAATATTTGAATAAACAAGATGAAGGATTTAAAAATAATGCCTTAGCTGACGCTTTAGCTAAACTAAAAAATGATGAA
TAA

Upstream 100 bases:

>100_bases
TGGTGTGATTGCATCATGCCGTACAGTTTGAGGAAAATATGTTACAGTAAACAATGAAATAATATAGATAATAATTATCT
TCGAAATGAGGAGTACATAA

Downstream 100 bases:

>100_bases
AGTATGAAAAACTGTCTCGTATAGAGGCAGTTTTTTTTATGGGAAAATAACGGATTTATCCTATTTTTAGAAATATTTTA
AAGATGATACTAATTACTTA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSKTVVLAEKPSVGRDIAKVLGCHKKGNGFLEGSKYIVTWALGHLVTLADPDMYDSKYKTWRMEDLPMLPDHLKLVVMKK
TGKQFQSVKTQLNRQDVVEIVIATDAGREGELVARWIIEKARVKKPIKRLWISSVTEKAIKDGFKQLKPGKNYWNLYQSA
VARSEADWYVGLNATRALTTKHNAQLSSGRVQTPTLEMVSLREQEIKNFQPKKFYQLEAKTTKGHRFLWKNDENQNRIFS
KNEAIKLEEKLSNKQATVVHVDKKLKKKHAQGLYDLTDLQRDANRIFQFSGKQTLSVMQKLYEQHKLVTYPRTDSRHLST
DIVSTLKDRVKACGVDEYAKIANTILNKELRLNKSVVDNAKVTDHHAIIPTEVPVVWTALDDKEKKIYHLIVQRFLAALS
DPMEYEQTTITIDIAGEQFIANGKVTKRLGWKEIYKHNDEEDEIQPLNVTKGSKLTLKVELKEGETKPPERFTEGTILQA
MENPTRYMGTNKKELVDTIKETGGIGTVATRADIIEKLFMNSYIELKGRNIYLTQTGRQLLDLAPEELRSPLLTAEWEQK
LSLIAEGKLKKDQFIKEIKSYTKEAVTEIKQSSETFKHDNMTGTKCPKCGKLMLEIKNKHGKKLVCQDRSCGGKKNIAKF
TNARCPNCHKRLELRGEGEGQIFTCKCGHREKLSTFQARKEKEKKQRVSKKDVKKYLNKQDEGFKNNALADALAKLKNDE

Sequences:

>Translated_720_residues
MSKTVVLAEKPSVGRDIAKVLGCHKKGNGFLEGSKYIVTWALGHLVTLADPDMYDSKYKTWRMEDLPMLPDHLKLVVMKK
TGKQFQSVKTQLNRQDVVEIVIATDAGREGELVARWIIEKARVKKPIKRLWISSVTEKAIKDGFKQLKPGKNYWNLYQSA
VARSEADWYVGLNATRALTTKHNAQLSSGRVQTPTLEMVSLREQEIKNFQPKKFYQLEAKTTKGHRFLWKNDENQNRIFS
KNEAIKLEEKLSNKQATVVHVDKKLKKKHAQGLYDLTDLQRDANRIFQFSGKQTLSVMQKLYEQHKLVTYPRTDSRHLST
DIVSTLKDRVKACGVDEYAKIANTILNKELRLNKSVVDNAKVTDHHAIIPTEVPVVWTALDDKEKKIYHLIVQRFLAALS
DPMEYEQTTITIDIAGEQFIANGKVTKRLGWKEIYKHNDEEDEIQPLNVTKGSKLTLKVELKEGETKPPERFTEGTILQA
MENPTRYMGTNKKELVDTIKETGGIGTVATRADIIEKLFMNSYIELKGRNIYLTQTGRQLLDLAPEELRSPLLTAEWEQK
LSLIAEGKLKKDQFIKEIKSYTKEAVTEIKQSSETFKHDNMTGTKCPKCGKLMLEIKNKHGKKLVCQDRSCGGKKNIAKF
TNARCPNCHKRLELRGEGEGQIFTCKCGHREKLSTFQARKEKEKKQRVSKKDVKKYLNKQDEGFKNNALADALAKLKNDE
>Mature_719_residues
SKTVVLAEKPSVGRDIAKVLGCHKKGNGFLEGSKYIVTWALGHLVTLADPDMYDSKYKTWRMEDLPMLPDHLKLVVMKKT
GKQFQSVKTQLNRQDVVEIVIATDAGREGELVARWIIEKARVKKPIKRLWISSVTEKAIKDGFKQLKPGKNYWNLYQSAV
ARSEADWYVGLNATRALTTKHNAQLSSGRVQTPTLEMVSLREQEIKNFQPKKFYQLEAKTTKGHRFLWKNDENQNRIFSK
NEAIKLEEKLSNKQATVVHVDKKLKKKHAQGLYDLTDLQRDANRIFQFSGKQTLSVMQKLYEQHKLVTYPRTDSRHLSTD
IVSTLKDRVKACGVDEYAKIANTILNKELRLNKSVVDNAKVTDHHAIIPTEVPVVWTALDDKEKKIYHLIVQRFLAALSD
PMEYEQTTITIDIAGEQFIANGKVTKRLGWKEIYKHNDEEDEIQPLNVTKGSKLTLKVELKEGETKPPERFTEGTILQAM
ENPTRYMGTNKKELVDTIKETGGIGTVATRADIIEKLFMNSYIELKGRNIYLTQTGRQLLDLAPEELRSPLLTAEWEQKL
SLIAEGKLKKDQFIKEIKSYTKEAVTEIKQSSETFKHDNMTGTKCPKCGKLMLEIKNKHGKKLVCQDRSCGGKKNIAKFT
NARCPNCHKRLELRGEGEGQIFTCKCGHREKLSTFQARKEKEKKQRVSKKDVKKYLNKQDEGFKNNALADALAKLKNDE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=642, Percent_Identity=26.9470404984424, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI4507635, Length=674, Percent_Identity=24.4807121661721, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1788061, Length=614, Percent_Identity=33.2247557003257, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787529, Length=660, Percent_Identity=27.5757575757576, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32563869, Length=684, Percent_Identity=25.1461988304094, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=627, Percent_Identity=25.6778309409888, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=660, Percent_Identity=23.9393939393939, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585251, Length=582, Percent_Identity=27.319587628866, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640096, Length=716, Percent_Identity=24.5810055865922, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 82363; Mature: 82232

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVVLAEKPSVGRDIAKVLGCHKKGNGFLEGSKYIVTWALGHLVTLADPDMYDSKYKT
CCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHEEEECCCCCCCCCCCE
WRMEDLPMLPDHLKLVVMKKTGKQFQSVKTQLNRQDVVEIVIATDAGREGELVARWIIEK
EECCCCCCCCCHHEEEEEHHHHHHHHHHHHHHCHHCEEEEEEEECCCCCCHHHHHHHHHH
ARVKKPIKRLWISSVTEKAIKDGFKQLKPGKNYWNLYQSAVARSEADWYVGLNATRALTT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEC
KHNAQLSSGRVQTPTLEMVSLREQEIKNFQPKKFYQLEAKTTKGHRFLWKNDENQNRIFS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEC
KNEAIKLEEKLSNKQATVVHVDKKLKKKHAQGLYDLTDLQRDANRIFQFSGKQTLSVMQK
CCCCEEEHHHHCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LYEQHKLVTYPRTDSRHLSTDIVSTLKDRVKACGVDEYAKIANTILNKELRLNKSVVDNA
HHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHCCC
KVTDHHAIIPTEVPVVWTALDDKEKKIYHLIVQRFLAALSDPMEYEQTTITIDIAGEQFI
CCCCCCEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEE
ANGKVTKRLGWKEIYKHNDEEDEIQPLNVTKGSKLTLKVELKEGETKPPERFTEGTILQA
ECCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCHHHHH
MENPTRYMGTNKKELVDTIKETGGIGTVATRADIIEKLFMNSYIELKGRNIYLTQTGRQL
HCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEECCCHHH
LDLAPEELRSPLLTAEWEQKLSLIAEGKLKKDQFIKEIKSYTKEAVTEIKQSSETFKHDN
HHCCCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MTGTKCPKCGKLMLEIKNKHGKKLVCQDRSCGGKKNIAKFTNARCPNCHKRLELRGEGEG
CCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHCEECCCCCC
QIFTCKCGHREKLSTFQARKEKEKKQRVSKKDVKKYLNKQDEGFKNNALADALAKLKNDE
CEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKTVVLAEKPSVGRDIAKVLGCHKKGNGFLEGSKYIVTWALGHLVTLADPDMYDSKYKT
CCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHEEEECCCCCCCCCCCE
WRMEDLPMLPDHLKLVVMKKTGKQFQSVKTQLNRQDVVEIVIATDAGREGELVARWIIEK
EECCCCCCCCCHHEEEEEHHHHHHHHHHHHHHCHHCEEEEEEEECCCCCCHHHHHHHHHH
ARVKKPIKRLWISSVTEKAIKDGFKQLKPGKNYWNLYQSAVARSEADWYVGLNATRALTT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEC
KHNAQLSSGRVQTPTLEMVSLREQEIKNFQPKKFYQLEAKTTKGHRFLWKNDENQNRIFS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEC
KNEAIKLEEKLSNKQATVVHVDKKLKKKHAQGLYDLTDLQRDANRIFQFSGKQTLSVMQK
CCCCEEEHHHHCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LYEQHKLVTYPRTDSRHLSTDIVSTLKDRVKACGVDEYAKIANTILNKELRLNKSVVDNA
HHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHCCC
KVTDHHAIIPTEVPVVWTALDDKEKKIYHLIVQRFLAALSDPMEYEQTTITIDIAGEQFI
CCCCCCEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEE
ANGKVTKRLGWKEIYKHNDEEDEIQPLNVTKGSKLTLKVELKEGETKPPERFTEGTILQA
ECCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCHHHHH
MENPTRYMGTNKKELVDTIKETGGIGTVATRADIIEKLFMNSYIELKGRNIYLTQTGRQL
HCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEECCCHHH
LDLAPEELRSPLLTAEWEQKLSLIAEGKLKKDQFIKEIKSYTKEAVTEIKQSSETFKHDN
HHCCCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MTGTKCPKCGKLMLEIKNKHGKKLVCQDRSCGGKKNIAKFTNARCPNCHKRLELRGEGEG
CCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHCEECCCCCC
QIFTCKCGHREKLSTFQARKEKEKKQRVSKKDVKKYLNKQDEGFKNNALADALAKLKNDE
CEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA