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The map label for this gene is iaaH [H]

Identifier: 23100038

GI number: 23100038

Start: 2641920

End: 2643230

Strand: Reverse

Name: iaaH [H]

Synonym: OB2583

Alternate gene names: 23100038

Gene position: 2643230-2641920 (Counterclockwise)

Preceding gene: 23100039

Following gene: 23100037

Centisome position: 72.81

GC content: 36.92

Gene sequence:

>1311_bases
ATGTCTGAATTTATTCACAATCTAACTCAACAATTAGCTCAATGGCGTAGAATGTTTCATTCTTATCCAGAACTTGGATT
TACAGAATATTTAACTACTTACCGCATAGGAAAAATGTTAGAGGAATGGAATTATTCGTTGTTTGTTGGTAAAGAAGCTC
TTGATTCTGATGCTCGAGTAGGTATACCAAATACTACCTTACTAACTCATTTTGAACAAAAGGCAAAAGAGCATGGTGTA
GAAGATTCTTGGCTTTGTAAAATGACTGGTGGAAATACGGGACTAGTAGCTACCTTTGATACTGGTAGACCAGGCAAACA
TATTGCTCTTCGATTTGATATAGATGCTTTACCAATAAATGAATCCACACATCAATCTCATATACCAGCAAGCGAAGCGT
TTAATTCCATAAATTCAAGAGTGATGCACGCATGTGGTCATGATGGCCATATAACGATTGGGTTAGGTGTTGCCAAGTAT
ATCTATGAACATCAGAGTCAGTTAGTTGGGAAATTTACCTTCTTATTCCAACCGGCCGAAGAGGGCGGTCGAGGAGCATT
AGCGATGACGAAAAAAGGCTGGTTAGCGGGTGTTGATCGTTTTTATGCTGGTCATATTGGAATAAAAGAAATGCCGGTTG
GAACTGTTGCTGCAACTGTTAAAGGATTTCTTGCTTCTACTAAACTGAATGTTCGTTATCATGGAAATGCATCACATGCT
GGAATAAATCCGGAAGCAGGAAAAAATGCACTACTTTCTGCGTCATCAGCGGCAATACATCTATATGGTATACCTAGACA
TAGTGCAGGAACAACTCGAGTAAATGTTGGTAAATTAGAAGCGGGAATTGGTAGAAATATAATTCCTGATGAAGCTTTTA
TGGAGATCGAGGTGCGCGGAGAGACAAAAGAATTAAATAATTATATGGCAGATCATGCCAAAAGAATCATAAATGCTGCT
GCTGAAATGCATGATACGAAATGTGAAATAACTACTGTTGGATCAACAGAGAAAATGAGTTGTAGTTCATCCATAATAAA
TGATATTAAAAATGCTTGTGAAAAAAGTAAGTATGTCAAAAATGTCATATCAGAAACATCGGTTTCTGGATCTGAAGATG
CTAGTTTTATGATGAATGAAGTACAAAAACATGGTGGACAAGCAACATATATGCTGTTTGGTACCAAATTAGATTATCCG
CATCATCATCCGTCTTTTGATTTTCAGGAAGAGGTATTACCAGTTGCTGTTGATACGTTTACTAGAATAATAGAACAAGA
AAATACGTATGTGCTGAATAGAGGTAGATAA

Upstream 100 bases:

>100_bases
AGTAAATTCCAAATTGATGAGCGAGCATTACCAAAAGGGACCAAATTGTTAGTTCAATTAGCACTCGATGCTTTAAAGGA
GTTGAAGCAGGAGGAAGATT

Downstream 100 bases:

>100_bases
TGTGAAAATAAAAATTGGAGTTATTGGACCTTCCGATTCCGTGAATCAAATAATGGCTGTGGCAAAAGAATTTCCAAATA
CTGAAATTACCCCTTTTACG

Product: indole-3-acetyl-L-aspartic acid hydrolase

Products: NA

Alternate protein names: IAA-Asp hydrolase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSEFIHNLTQQLAQWRRMFHSYPELGFTEYLTTYRIGKMLEEWNYSLFVGKEALDSDARVGIPNTTLLTHFEQKAKEHGV
EDSWLCKMTGGNTGLVATFDTGRPGKHIALRFDIDALPINESTHQSHIPASEAFNSINSRVMHACGHDGHITIGLGVAKY
IYEHQSQLVGKFTFLFQPAEEGGRGALAMTKKGWLAGVDRFYAGHIGIKEMPVGTVAATVKGFLASTKLNVRYHGNASHA
GINPEAGKNALLSASSAAIHLYGIPRHSAGTTRVNVGKLEAGIGRNIIPDEAFMEIEVRGETKELNNYMADHAKRIINAA
AEMHDTKCEITTVGSTEKMSCSSSIINDIKNACEKSKYVKNVISETSVSGSEDASFMMNEVQKHGGQATYMLFGTKLDYP
HHHPSFDFQEEVLPVAVDTFTRIIEQENTYVLNRGR

Sequences:

>Translated_436_residues
MSEFIHNLTQQLAQWRRMFHSYPELGFTEYLTTYRIGKMLEEWNYSLFVGKEALDSDARVGIPNTTLLTHFEQKAKEHGV
EDSWLCKMTGGNTGLVATFDTGRPGKHIALRFDIDALPINESTHQSHIPASEAFNSINSRVMHACGHDGHITIGLGVAKY
IYEHQSQLVGKFTFLFQPAEEGGRGALAMTKKGWLAGVDRFYAGHIGIKEMPVGTVAATVKGFLASTKLNVRYHGNASHA
GINPEAGKNALLSASSAAIHLYGIPRHSAGTTRVNVGKLEAGIGRNIIPDEAFMEIEVRGETKELNNYMADHAKRIINAA
AEMHDTKCEITTVGSTEKMSCSSSIINDIKNACEKSKYVKNVISETSVSGSEDASFMMNEVQKHGGQATYMLFGTKLDYP
HHHPSFDFQEEVLPVAVDTFTRIIEQENTYVLNRGR
>Mature_435_residues
SEFIHNLTQQLAQWRRMFHSYPELGFTEYLTTYRIGKMLEEWNYSLFVGKEALDSDARVGIPNTTLLTHFEQKAKEHGVE
DSWLCKMTGGNTGLVATFDTGRPGKHIALRFDIDALPINESTHQSHIPASEAFNSINSRVMHACGHDGHITIGLGVAKYI
YEHQSQLVGKFTFLFQPAEEGGRGALAMTKKGWLAGVDRFYAGHIGIKEMPVGTVAATVKGFLASTKLNVRYHGNASHAG
INPEAGKNALLSASSAAIHLYGIPRHSAGTTRVNVGKLEAGIGRNIIPDEAFMEIEVRGETKELNNYMADHAKRIINAAA
EMHDTKCEITTVGSTEKMSCSSSIINDIKNACEKSKYVKNVISETSVSGSEDASFMMNEVQKHGGQATYMLFGTKLDYPH
HHPSFDFQEEVLPVAVDTFTRIIEQENTYVLNRGR

Specific function: Hydrolyzes indole-3-acetyl-N-aspartic acid to indole-3- acetic acid [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=41.371158392435, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 48170; Mature: 48039

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFIHNLTQQLAQWRRMFHSYPELGFTEYLTTYRIGKMLEEWNYSLFVGKEALDSDARV
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCC
GIPNTTLLTHFEQKAKEHGVEDSWLCKMTGGNTGLVATFDTGRPGKHIALRFDIDALPIN
CCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEEECEEECCC
ESTHQSHIPASEAFNSINSRVMHACGHDGHITIGLGVAKYIYEHQSQLVGKFTFLFQPAE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHEEEEEEECCC
EGGRGALAMTKKGWLAGVDRFYAGHIGIKEMPVGTVAATVKGFLASTKLNVRYHGNASHA
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCC
GINPEAGKNALLSASSAAIHLYGIPRHSAGTTRVNVGKLEAGIGRNIIPDEAFMEIEVRG
CCCCCCCCCCEEECCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECC
ETKELNNYMADHAKRIINAAAEMHDTKCEITTVGSTEKMSCSSSIINDIKNACEKSKYVK
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
NVISETSVSGSEDASFMMNEVQKHGGQATYMLFGTKLDYPHHHPSFDFQEEVLPVAVDTF
HHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
TRIIEQENTYVLNRGR
HHHHHCCCCEEEECCC
>Mature Secondary Structure 
SEFIHNLTQQLAQWRRMFHSYPELGFTEYLTTYRIGKMLEEWNYSLFVGKEALDSDARV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCC
GIPNTTLLTHFEQKAKEHGVEDSWLCKMTGGNTGLVATFDTGRPGKHIALRFDIDALPIN
CCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEEECEEECCC
ESTHQSHIPASEAFNSINSRVMHACGHDGHITIGLGVAKYIYEHQSQLVGKFTFLFQPAE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHEEEEEEECCC
EGGRGALAMTKKGWLAGVDRFYAGHIGIKEMPVGTVAATVKGFLASTKLNVRYHGNASHA
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCC
GINPEAGKNALLSASSAAIHLYGIPRHSAGTTRVNVGKLEAGIGRNIIPDEAFMEIEVRG
CCCCCCCCCCEEECCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECC
ETKELNNYMADHAKRIINAAAEMHDTKCEITTVGSTEKMSCSSSIINDIKNACEKSKYVK
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
NVISETSVSGSEDASFMMNEVQKHGGQATYMLFGTKLDYPHHHPSFDFQEEVLPVAVDTF
HHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
TRIIEQENTYVLNRGR
HHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9747708 [H]