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The map label for this gene is yclK [H]

Identifier: 23099969

GI number: 23099969

Start: 2570315

End: 2571772

Strand: Reverse

Name: yclK [H]

Synonym: OB2514

Alternate gene names: 23099969

Gene position: 2571772-2570315 (Counterclockwise)

Preceding gene: 23099970

Following gene: 23099968

Centisome position: 70.84

GC content: 34.98

Gene sequence:

>1458_bases
GTGGATTGGGGTATCAATTCTGTGTTTATAAATAAGTCTAAACGAATTCCTATGCGACGTTACTGGACTAGTCGTTATCT
TATAACATTATGTGTTGGTTTATTGATTATAGCAATTTTATCTGTATGGTGGATTCGTCATAGTACACTAGAAAATCGTG
TACAAATGATGGAATTATTAACCGAAGATATGGCAGATCGTTACACAGAATCAAGGGGAGAGAGACCACAGCCAGGGGGA
ATTCCTGGTGGTTTCCCTGATCAACGTTGGATGGGATTGGAACAAGAGCCTCTAATTTATATAATTGATCGTTCAGGAGA
AATCATTTCTAGGAATCGGCCAATGAGTCCACTTGATCAAGAGATAGATATAGAAATGTTAGATTCTACAGAAACGGTAC
AAACACTGAAAATGGATGATCAAAACCGATTAACTTTCTATATGGTAAAATCCCCGATCGTTATGGATGATACAACGATT
GGCTGGGTGATGATGGCAGAATCGAAACAATTATTAACAAAGGTAAACCATGAATATAAATTATTAGCTACGATGATTAT
TAGTCTTGCTTTATTAGGCTGGGCGGCTATCTATATATTGACAAAACGCTTATCAGATCCAATTAAACAGGTCGCTAATG
CAGCTAGGAACATAGAAGAGGGAGACTACAAAGTTGAGTTATCAAATAATAGTAAAGAAGAAGAAATTCATGAATTAGTC
TCGTCTTTTAAAGGAATGGCCAATCGATTGGAAAGGCTAGAAGCTATTCGTACGGAATTATTAGCTGGAGTAACGCATGA
ATTAAAAACACCAGTTACTTCAATTAGTGGATTATTACAAGCAATCAATGATGATGTTGTGAATGATGAAGAGAGAAAGG
AATTCCTAGAGATTTCACTGAAAGAAACAGAAAAAATGCAAACAATGATTGAAGATTTACTCGCTTTTAACTCTTTTGCA
TCAAGTACATTACCTTTAAATCGAGAGAAAGTAGCTATTAATGAGACGATTGAAGAAATTGTACATGATTGGGAAATTAC
GAATCTGAATAATTCTATTCGTGTAGAAGTGGAAAGAAATAGTGAAGAGGCTATAGTAGAAGTTGATTGGATTCGATTAG
AACAGATTATAACGAACTTATTTAATAACGCTAGTCAAGCAATGTCTGAAGGGATCATAACCGTAAAAATTCTAATCGAA
CAACAAGATGTATGGATATATGTTGTTGATGGTGGAATTGGGATTCCAATGGAAGAGCAAGCTTTAATTTTTGAGAGGTT
CTATCGTGGTAATGAGAAGAAATATTCAACGAGAGGATTAGGGTTAGGATTAGCATTAAGTAAAATGATTGCACAAGCAA
TGAATGGAGACTTAACTTTACTCCATAGTGATAAAAGTGGAACAACGATGAAATTGCAATTACCCATAGTACAAGAAAAC
AGAAAACAATCAGATTGA

Upstream 100 bases:

>100_bases
GATTATGATGGTAGTGATCGAGCAGTAGATTTATCGATTAAACGGTTACGTAAATCATTAAAAGACTGGCCAAAATCTGA
AGGAGAAATAAAAACATTTC

Downstream 100 bases:

>100_bases
AGCCGAAGTATTATCCCTTTGCGGAAGATACTTCGGTATTTTTTGTTTAAAAATGTTGACGGAACCATTAATAATTAATC
TAGACATTTTCCTGACACAT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDWGINSVFINKSKRIPMRRYWTSRYLITLCVGLLIIAILSVWWIRHSTLENRVQMMELLTEDMADRYTESRGERPQPGG
IPGGFPDQRWMGLEQEPLIYIIDRSGEIISRNRPMSPLDQEIDIEMLDSTETVQTLKMDDQNRLTFYMVKSPIVMDDTTI
GWVMMAESKQLLTKVNHEYKLLATMIISLALLGWAAIYILTKRLSDPIKQVANAARNIEEGDYKVELSNNSKEEEIHELV
SSFKGMANRLERLEAIRTELLAGVTHELKTPVTSISGLLQAINDDVVNDEERKEFLEISLKETEKMQTMIEDLLAFNSFA
SSTLPLNREKVAINETIEEIVHDWEITNLNNSIRVEVERNSEEAIVEVDWIRLEQIITNLFNNASQAMSEGIITVKILIE
QQDVWIYVVDGGIGIPMEEQALIFERFYRGNEKKYSTRGLGLGLALSKMIAQAMNGDLTLLHSDKSGTTMKLQLPIVQEN
RKQSD

Sequences:

>Translated_485_residues
MDWGINSVFINKSKRIPMRRYWTSRYLITLCVGLLIIAILSVWWIRHSTLENRVQMMELLTEDMADRYTESRGERPQPGG
IPGGFPDQRWMGLEQEPLIYIIDRSGEIISRNRPMSPLDQEIDIEMLDSTETVQTLKMDDQNRLTFYMVKSPIVMDDTTI
GWVMMAESKQLLTKVNHEYKLLATMIISLALLGWAAIYILTKRLSDPIKQVANAARNIEEGDYKVELSNNSKEEEIHELV
SSFKGMANRLERLEAIRTELLAGVTHELKTPVTSISGLLQAINDDVVNDEERKEFLEISLKETEKMQTMIEDLLAFNSFA
SSTLPLNREKVAINETIEEIVHDWEITNLNNSIRVEVERNSEEAIVEVDWIRLEQIITNLFNNASQAMSEGIITVKILIE
QQDVWIYVVDGGIGIPMEEQALIFERFYRGNEKKYSTRGLGLGLALSKMIAQAMNGDLTLLHSDKSGTTMKLQLPIVQEN
RKQSD
>Mature_485_residues
MDWGINSVFINKSKRIPMRRYWTSRYLITLCVGLLIIAILSVWWIRHSTLENRVQMMELLTEDMADRYTESRGERPQPGG
IPGGFPDQRWMGLEQEPLIYIIDRSGEIISRNRPMSPLDQEIDIEMLDSTETVQTLKMDDQNRLTFYMVKSPIVMDDTTI
GWVMMAESKQLLTKVNHEYKLLATMIISLALLGWAAIYILTKRLSDPIKQVANAARNIEEGDYKVELSNNSKEEEIHELV
SSFKGMANRLERLEAIRTELLAGVTHELKTPVTSISGLLQAINDDVVNDEERKEFLEISLKETEKMQTMIEDLLAFNSFA
SSTLPLNREKVAINETIEEIVHDWEITNLNNSIRVEVERNSEEAIVEVDWIRLEQIITNLFNNASQAMSEGIITVKILIE
QQDVWIYVVDGGIGIPMEEQALIFERFYRGNEKKYSTRGLGLGLALSKMIAQAMNGDLTLLHSDKSGTTMKLQLPIVQEN
RKQSD

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=318, Percent_Identity=25.4716981132075, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788713, Length=266, Percent_Identity=23.6842105263158, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788393, Length=339, Percent_Identity=25.9587020648968, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786783, Length=300, Percent_Identity=24.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994928, Length=246, Percent_Identity=26.0162601626016, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=25.8064516129032, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081816, Length=249, Percent_Identity=25.3012048192771, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=25.0965250965251, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=238, Percent_Identity=27.7310924369748, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=27.2340425531915, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789808, Length=313, Percent_Identity=24.2811501597444, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790861, Length=248, Percent_Identity=29.0322580645161, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786600, Length=281, Percent_Identity=24.9110320284698, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=346, Percent_Identity=23.121387283237, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=280, Percent_Identity=25.3571428571429, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787374, Length=301, Percent_Identity=25.2491694352159, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55546; Mature: 55546

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWGINSVFINKSKRIPMRRYWTSRYLITLCVGLLIIAILSVWWIRHSTLENRVQMMELL
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEDMADRYTESRGERPQPGGIPGGFPDQRWMGLEQEPLIYIIDRSGEIISRNRPMSPLDQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCC
EIDIEMLDSTETVQTLKMDDQNRLTFYMVKSPIVMDDTTIGWVMMAESKQLLTKVNHEYK
CCCEEECCCCHHHHHEECCCCCCEEEEEECCCEEECCCCEEEEEEECHHHHHHHHCCHHH
LLATMIISLALLGWAAIYILTKRLSDPIKQVANAARNIEEGDYKVELSNNSKEEEIHELV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
SSFKGMANRLERLEAIRTELLAGVTHELKTPVTSISGLLQAINDDVVNDEERKEFLEISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KETEKMQTMIEDLLAFNSFASSTLPLNREKVAINETIEEIVHDWEITNLNNSIRVEVERN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCEEEEEEECC
SEEAIVEVDWIRLEQIITNLFNNASQAMSEGIITVKILIEQQDVWIYVVDGGIGIPMEEQ
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCCCCCCHH
ALIFERFYRGNEKKYSTRGLGLGLALSKMIAQAMNGDLTLLHSDKSGTTMKLQLPIVQEN
HHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECHHHCC
RKQSD
CCCCC
>Mature Secondary Structure
MDWGINSVFINKSKRIPMRRYWTSRYLITLCVGLLIIAILSVWWIRHSTLENRVQMMELL
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEDMADRYTESRGERPQPGGIPGGFPDQRWMGLEQEPLIYIIDRSGEIISRNRPMSPLDQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCC
EIDIEMLDSTETVQTLKMDDQNRLTFYMVKSPIVMDDTTIGWVMMAESKQLLTKVNHEYK
CCCEEECCCCHHHHHEECCCCCCEEEEEECCCEEECCCCEEEEEEECHHHHHHHHCCHHH
LLATMIISLALLGWAAIYILTKRLSDPIKQVANAARNIEEGDYKVELSNNSKEEEIHELV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
SSFKGMANRLERLEAIRTELLAGVTHELKTPVTSISGLLQAINDDVVNDEERKEFLEISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KETEKMQTMIEDLLAFNSFASSTLPLNREKVAINETIEEIVHDWEITNLNNSIRVEVERN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCEEEEEEECC
SEEAIVEVDWIRLEQIITNLFNNASQAMSEGIITVKILIEQQDVWIYVVDGGIGIPMEEQ
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCCCCCCHH
ALIFERFYRGNEKKYSTRGLGLGLALSKMIAQAMNGDLTLLHSDKSGTTMKLQLPIVQEN
HHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECHHHCC
RKQSD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]