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The map label for this gene is ytoP [H]

Identifier: 23099693

GI number: 23099693

Start: 2277837

End: 2278907

Strand: Reverse

Name: ytoP [H]

Synonym: OB2238

Alternate gene names: 23099693

Gene position: 2278907-2277837 (Counterclockwise)

Preceding gene: 23099695

Following gene: 23099692

Centisome position: 62.77

GC content: 38.94

Gene sequence:

>1071_bases
ATGAGAGTGGAGAGAGAAACGCTATCGCTTTTTAAAACATTAACGGAACTACAAGGTGCACCTGGTAATGAACACCCGGT
ACGTAATTTTATGAAGGGTGAATTAGAAAAATATTCGGATGAGGTTATACAAGATAATCTAGGCGGAACTTTTGGTGTGA
AGCATGGGAATGGACCTAGAGTTATGGTGGCAGGTCACATGGATGAAGTTGGATTTATGGTCACACAAATTACAGATAAC
GGCATGTTACGCTTTCAACCACTTGGTGGTTGGTGGAATCAAGTTATGCTTGCACAAAGAGTTCAGGTTATGACTGCAAA
CGGACCGGTTATTGGGGTTATTGGTTCGATACCGCCCCATTTACTTACGGAGGCGCAACGCAGTAAACCAATGGAAATTA
AAAATATGCTTATTGATATTGGTGCAGACGATAAAGAAGATGTAAAGAAAATAGGTGTAAAGCCAGGCGACAGTATCGTT
CCAATCACACCATTCGAGCCTATGGCCAATGAAAAAAAGATTTTAGCAAAAGCTTGGGATAATCGTTATGGATGCGGATT
AGCTATTGAATTGTTAAAAGAGTTACAAAATGAACAGCTACCAAATCAGCTATATTCTGGAGCAACGGTACAGGAAGAAG
TTGGACTTAGAGGAGCACAAGTAGCGGCAAATATGATTCAACCTGATGTGTTCTATGCATTAGATGCATCTCCAGCCAAT
GATGCTTCTGGAGATAAACAAGCATTTGGTCAATTAGGAAAAGGAGCATTACTTCGTATATTTGATCGAACAATGGTAAC
ACATAATGGTATGCGAGAATATGTACTTGATACTGCAGAAAGCAATAATATTCCATATCAGTATTTTATCTCACAAGGAG
GAACAGATGCAGGTCGTGTACATCTTGCTAATGATGGAGTTCCCTCTGCAGTAGTCGGAATTTGTTCACGCTATATTCAC
ACTTCAGCGTCTATTATTCATGTAGATGATTATGCAGCTGCAAAAGAATTGCTTATAAAATTAGTTAAAGGTACAGATAG
TTCCTTCGTATCCCAATTAAAAGAAAAATAA

Upstream 100 bases:

>100_bases
AAAATGACAACGATTTGATATGATTACTGGAAAGATTTTGACTTTTTCAGTTATACTACTAATTAGATGTTACATAGCAT
TAAAAAATAGAAAGGGGAGT

Downstream 100 bases:

>100_bases
AGAGATGAGCAGTTGCCTTTAGGTAGCTGCTCTTTCATAGGAGATTATTAATATGCAAATACAAGTAGGATCACAAAATC
CAACGAAAATTCAAGCAGTA

Product: endo-1,4-beta-glucanase

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRVERETLSLFKTLTELQGAPGNEHPVRNFMKGELEKYSDEVIQDNLGGTFGVKHGNGPRVMVAGHMDEVGFMVTQITDN
GMLRFQPLGGWWNQVMLAQRVQVMTANGPVIGVIGSIPPHLLTEAQRSKPMEIKNMLIDIGADDKEDVKKIGVKPGDSIV
PITPFEPMANEKKILAKAWDNRYGCGLAIELLKELQNEQLPNQLYSGATVQEEVGLRGAQVAANMIQPDVFYALDASPAN
DASGDKQAFGQLGKGALLRIFDRTMVTHNGMREYVLDTAESNNIPYQYFISQGGTDAGRVHLANDGVPSAVVGICSRYIH
TSASIIHVDDYAAAKELLIKLVKGTDSSFVSQLKEK

Sequences:

>Translated_356_residues
MRVERETLSLFKTLTELQGAPGNEHPVRNFMKGELEKYSDEVIQDNLGGTFGVKHGNGPRVMVAGHMDEVGFMVTQITDN
GMLRFQPLGGWWNQVMLAQRVQVMTANGPVIGVIGSIPPHLLTEAQRSKPMEIKNMLIDIGADDKEDVKKIGVKPGDSIV
PITPFEPMANEKKILAKAWDNRYGCGLAIELLKELQNEQLPNQLYSGATVQEEVGLRGAQVAANMIQPDVFYALDASPAN
DASGDKQAFGQLGKGALLRIFDRTMVTHNGMREYVLDTAESNNIPYQYFISQGGTDAGRVHLANDGVPSAVVGICSRYIH
TSASIIHVDDYAAAKELLIKLVKGTDSSFVSQLKEK
>Mature_356_residues
MRVERETLSLFKTLTELQGAPGNEHPVRNFMKGELEKYSDEVIQDNLGGTFGVKHGNGPRVMVAGHMDEVGFMVTQITDN
GMLRFQPLGGWWNQVMLAQRVQVMTANGPVIGVIGSIPPHLLTEAQRSKPMEIKNMLIDIGADDKEDVKKIGVKPGDSIV
PITPFEPMANEKKILAKAWDNRYGCGLAIELLKELQNEQLPNQLYSGATVQEEVGLRGAQVAANMIQPDVFYALDASPAN
DASGDKQAFGQLGKGALLRIFDRTMVTHNGMREYVLDTAESNNIPYQYFISQGGTDAGRVHLANDGVPSAVVGICSRYIH
TSASIIHVDDYAAAKELLIKLVKGTDSSFVSQLKEK

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=349, Percent_Identity=39.2550143266476, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788727, Length=351, Percent_Identity=33.048433048433, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI87082417, Length=359, Percent_Identity=28.6908077994429, Blast_Score=151, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 38924; Mature: 38924

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERETLSLFKTLTELQGAPGNEHPVRNFMKGELEKYSDEVIQDNLGGTFGVKHGNGPR
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCE
VMVAGHMDEVGFMVTQITDNGMLRFQPLGGWWNQVMLAQRVQVMTANGPVIGVIGSIPPH
EEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHEEECCCCEEEEECCCCHH
LLTEAQRSKPMEIKNMLIDIGADDKEDVKKIGVKPGDSIVPITPFEPMANEKKILAKAWD
HHHHHHHCCCHHHHHHEEECCCCCHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHC
NRYGCGLAIELLKELQNEQLPNQLYSGATVQEEVGLRGAQVAANMIQPDVFYALDASPAN
CCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCCHHHHHHHHCCCCEEEEECCCCCC
DASGDKQAFGQLGKGALLRIFDRTMVTHNGMREYVLDTAESNNIPYQYFISQGGTDAGRV
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCEE
HLANDGVPSAVVGICSRYIHTSASIIHVDDYAAAKELLIKLVKGTDSSFVSQLKEK
EEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MRVERETLSLFKTLTELQGAPGNEHPVRNFMKGELEKYSDEVIQDNLGGTFGVKHGNGPR
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCE
VMVAGHMDEVGFMVTQITDNGMLRFQPLGGWWNQVMLAQRVQVMTANGPVIGVIGSIPPH
EEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHEEECCCCEEEEECCCCHH
LLTEAQRSKPMEIKNMLIDIGADDKEDVKKIGVKPGDSIVPITPFEPMANEKKILAKAWD
HHHHHHHCCCHHHHHHEEECCCCCHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHC
NRYGCGLAIELLKELQNEQLPNQLYSGATVQEEVGLRGAQVAANMIQPDVFYALDASPAN
CCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCCHHHHHHHHCCCCEEEEECCCCCC
DASGDKQAFGQLGKGALLRIFDRTMVTHNGMREYVLDTAESNNIPYQYFISQGGTDAGRV
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCEE
HLANDGVPSAVVGICSRYIHTSASIIHVDDYAAAKELLIKLVKGTDSSFVSQLKEK
EEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]