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The map label for this gene is ytpR [H]

Identifier: 23099688

GI number: 23099688

Start: 2274762

End: 2275370

Strand: Reverse

Name: ytpR [H]

Synonym: OB2233

Alternate gene names: 23099688

Gene position: 2275370-2274762 (Counterclockwise)

Preceding gene: 23099689

Following gene: 23099687

Centisome position: 62.67

GC content: 36.29

Gene sequence:

>609_bases
ATGGATGTATTTTATAATCCAAATGGAATTGGTGATGTATTACTCATCCCATTAAAAGACGGTAATCGATATGAAATGGA
ATCAACTAATTATGACAATGTAACACGAATAACTTCTGCTGACGGAGAATTGCTTGGATATAATATTTTCCAAGCTTCCC
AAGTATTGGACTTACCAAATGGAAATGGACCTATTCGTTTAAGTGAAGATTTATTAGAAAAAATACGAAAACTCTTTAGG
GAAAAAGGCATTAAAGACCCCCTTGATTTTGATTTGACTCCGAAGTTTGTAGTAGGGTATGTGACCACGAAAGAACCCCA
TGAAAATGCTGACAAACTAAGTGTATGTCAAGTTGATGTGGGAACAGAGAAGTTGCAAATTGTTTGTGGTGCACCAAATG
TTGATGCAAACCAAAAAGTGGTAGTTGCTAAAGTCGGTGCAACGATGCCAAGCGGAATGAAAATTAAAGACTCCGAACTC
CGAGGAGTACCTTCAAACGGAATGATTTGCTCCCAAAAAGAATTAGGAATCCCTAATGCACCAAAAGAAAAAGGAATTTA
TGTATTGGATGATTCTTATAACGTGGGAGAAGAATTCAAATTGTCTTAA

Upstream 100 bases:

>100_bases
CAATAACATCACTTTCCTTTGTGTATGAAGATAGAAAGTTGGAACCTATCTTTATTTTAGCGAAAAACAAACCAGAAAAA
TAAATAGGAGCTGAATAACA

Downstream 100 bases:

>100_bases
CACATGCCCCCTAAGCTATTATTGCAGGGGGATTTCTTGTGTCATTAGACCACCATTCTCCCACATCGAATGGATGATTC
CAAATAGGTTGAAAAACTGA

Product: phenylalanyl-tRNA synthetase beta subunit

Products: AMP; diphosphate; L-phenylalanyl-tRNA(Phe)

Alternate protein names: Partial phenylalanyl-tRNA synthetase; Partial PheRS; pFRS [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MDVFYNPNGIGDVLLIPLKDGNRYEMESTNYDNVTRITSADGELLGYNIFQASQVLDLPNGNGPIRLSEDLLEKIRKLFR
EKGIKDPLDFDLTPKFVVGYVTTKEPHENADKLSVCQVDVGTEKLQIVCGAPNVDANQKVVVAKVGATMPSGMKIKDSEL
RGVPSNGMICSQKELGIPNAPKEKGIYVLDDSYNVGEEFKLS

Sequences:

>Translated_202_residues
MDVFYNPNGIGDVLLIPLKDGNRYEMESTNYDNVTRITSADGELLGYNIFQASQVLDLPNGNGPIRLSEDLLEKIRKLFR
EKGIKDPLDFDLTPKFVVGYVTTKEPHENADKLSVCQVDVGTEKLQIVCGAPNVDANQKVVVAKVGATMPSGMKIKDSEL
RGVPSNGMICSQKELGIPNAPKEKGIYVLDDSYNVGEEFKLS
>Mature_202_residues
MDVFYNPNGIGDVLLIPLKDGNRYEMESTNYDNVTRITSADGELLGYNIFQASQVLDLPNGNGPIRLSEDLLEKIRKLFR
EKGIKDPLDFDLTPKFVVGYVTTKEPHENADKLSVCQVDVGTEKLQIVCGAPNVDANQKVVVAKVGATMPSGMKIKDSEL
RGVPSNGMICSQKELGIPNAPKEKGIYVLDDSYNVGEEFKLS

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=74, Percent_Identity=54.0540540540541, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.20

Molecular weight: Translated: 22197; Mature: 22197

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFYNPNGIGDVLLIPLKDGNRYEMESTNYDNVTRITSADGELLGYNIFQASQVLDLPN
CCEEECCCCCCCEEEEECCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCCEEEECCC
GNGPIRLSEDLLEKIRKLFREKGIKDPLDFDLTPKFVVGYVTTKEPHENADKLSVCQVDV
CCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEC
GTEKLQIVCGAPNVDANQKVVVAKVGATMPSGMKIKDSELRGVPSNGMICSQKELGIPNA
CCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCEEECHHCCCCCCC
PKEKGIYVLDDSYNVGEEFKLS
CCCCCEEEEECCCCCCCEEECC
>Mature Secondary Structure
MDVFYNPNGIGDVLLIPLKDGNRYEMESTNYDNVTRITSADGELLGYNIFQASQVLDLPN
CCEEECCCCCCCEEEEECCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCCEEEECCC
GNGPIRLSEDLLEKIRKLFREKGIKDPLDFDLTPKFVVGYVTTKEPHENADKLSVCQVDV
CCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEC
GTEKLQIVCGAPNVDANQKVVVAKVGATMPSGMKIKDSELRGVPSNGMICSQKELGIPNA
CCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCEEECHHCCCCCCC
PKEKGIYVLDDSYNVGEEFKLS
CCCCCEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; L-phenylalanine; tRNA(Phe)

Specific reaction: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]