Click here to switch to the map view.

The map label for this gene is murC

Identifier: 23099686

GI number: 23099686

Start: 2270425

End: 2271741

Strand: Reverse

Name: murC

Synonym: OB2231

Alternate gene names: 23099686

Gene position: 2271741-2270425 (Counterclockwise)

Preceding gene: 23099687

Following gene: 23099685

Centisome position: 62.57

GC content: 33.49

Gene sequence:

>1317_bases
ATGACAACTTACCATTTTATAGGTATAAAAGGAACTGGAATGAGTGCACTTGCACAAATTCTTCATGACTCTGGAGAAAA
AGTGCAAGGATCTGATTTTGAAAAGCGTTTCTTTACTCAAGAAGCGTTAGAGCAAAAAAATATTACTATTTTACCGTTTT
CTAAAGAAAATATTAAAGAAGATTATACAATAATTGCAGGTAATGCGTTTTCTGATGATCATATTGAAATTCAGAAGGCT
AAAGAGCTTGGATGTAAATTTTACCGTTATCATGAGTTTCTAGGTGAATGGCTAAAACAGTACACAAGTATTGCAGTCAC
TGGAGCGCATGGGAAAACTTCAACGACTGGTTTGTTATCACATGTTTTAAAATCTGCTTATCCAATCTCCTATTTAATTG
GGGATGGAACTGGCAATGGTCATGTAGATAGCGAATATTTTGTTTTTGAAGCATGTGAGTATCGTAGACACTTTTTAAAA
TACGAGCCTGATTATGCGATTATGACGAATATTGATTTCGATCATCCAGATTACTTTACAAGTTTAGATGATGTCGTTGA
TGCATTTCAGTCTATGGCAGATCGAGTTAAAAAGTGTATTGTGGCATGCGGTGATGATGAACAATTACAAGGAATTCATA
CAAAGGTACCTGTCATCTACTATGGTTTTAATGATTCTAATGATTTTCAGGCACAAAACGTAGTAGAAACAGCAAATGGC
ACTGAATTTGACGTATTTGTACGGAACACCTACTATGATCACTTTATAATTCCTACGTATGGAAATCATACGGTATTGAA
TGCACTGGCTGTTATTGCCATTTGTCATTATGAAGGGATTTCCGTTGAGCATATGAAACAATTGGATACCTTTGAAGGAG
TTAAACGTCGATTCACAGAAAAGGAAATAGGATCACAAATTGTTATTGATGATTATGCACATCACCCTAAAGAAATATCT
GTTACAATAGAGTCTGCAAGGAAAAAATATCCAAATAAAAATGTGGTAGCTATTTTCCAACCACATACATTTACTCGTAC
AAAAACATTCTTAAAAGAGTTTGCAGATAGTCTAAATGAAGCGGATAATGTATATCTGTGTGATATATTTAAATCGGCAC
GTGAAGATTCAGGACAGTTAACCATTAATGATTTGCAGAAATTAATTCCTAACAGTCAATTATTATCTTTAAAGGAAACA
GAAGTTTTACAAGAATTTAAAGATAGTGTTCTAATATTTATGGGAGCGGGAGATATTCAGAAATTCCAGGCTGCTTATGA
AGAAACAACTACTAAATCAACAAATTTTCAAGATTAA

Upstream 100 bases:

>100_bases
AGTTTGAAAGAGATCGATTTTTTGCTATAATGTACCGGTATGAAAAAGTACTAAACATCATAAGAATGAATGATTGTAAG
AACATGTTGGAGGTTCGTTT

Downstream 100 bases:

>100_bases
TTATGAACCGAGCTTTAGAAAATAAGGCTCGGTTTTTTGTATTTTTTAACATGAAAATGTTAGTATTTGTACAAATTATG
GGAAATAATATACAATGGAT

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTTYHFIGIKGTGMSALAQILHDSGEKVQGSDFEKRFFTQEALEQKNITILPFSKENIKEDYTIIAGNAFSDDHIEIQKA
KELGCKFYRYHEFLGEWLKQYTSIAVTGAHGKTSTTGLLSHVLKSAYPISYLIGDGTGNGHVDSEYFVFEACEYRRHFLK
YEPDYAIMTNIDFDHPDYFTSLDDVVDAFQSMADRVKKCIVACGDDEQLQGIHTKVPVIYYGFNDSNDFQAQNVVETANG
TEFDVFVRNTYYDHFIIPTYGNHTVLNALAVIAICHYEGISVEHMKQLDTFEGVKRRFTEKEIGSQIVIDDYAHHPKEIS
VTIESARKKYPNKNVVAIFQPHTFTRTKTFLKEFADSLNEADNVYLCDIFKSAREDSGQLTINDLQKLIPNSQLLSLKET
EVLQEFKDSVLIFMGAGDIQKFQAAYEETTTKSTNFQD

Sequences:

>Translated_438_residues
MTTYHFIGIKGTGMSALAQILHDSGEKVQGSDFEKRFFTQEALEQKNITILPFSKENIKEDYTIIAGNAFSDDHIEIQKA
KELGCKFYRYHEFLGEWLKQYTSIAVTGAHGKTSTTGLLSHVLKSAYPISYLIGDGTGNGHVDSEYFVFEACEYRRHFLK
YEPDYAIMTNIDFDHPDYFTSLDDVVDAFQSMADRVKKCIVACGDDEQLQGIHTKVPVIYYGFNDSNDFQAQNVVETANG
TEFDVFVRNTYYDHFIIPTYGNHTVLNALAVIAICHYEGISVEHMKQLDTFEGVKRRFTEKEIGSQIVIDDYAHHPKEIS
VTIESARKKYPNKNVVAIFQPHTFTRTKTFLKEFADSLNEADNVYLCDIFKSAREDSGQLTINDLQKLIPNSQLLSLKET
EVLQEFKDSVLIFMGAGDIQKFQAAYEETTTKSTNFQD
>Mature_437_residues
TTYHFIGIKGTGMSALAQILHDSGEKVQGSDFEKRFFTQEALEQKNITILPFSKENIKEDYTIIAGNAFSDDHIEIQKAK
ELGCKFYRYHEFLGEWLKQYTSIAVTGAHGKTSTTGLLSHVLKSAYPISYLIGDGTGNGHVDSEYFVFEACEYRRHFLKY
EPDYAIMTNIDFDHPDYFTSLDDVVDAFQSMADRVKKCIVACGDDEQLQGIHTKVPVIYYGFNDSNDFQAQNVVETANGT
EFDVFVRNTYYDHFIIPTYGNHTVLNALAVIAICHYEGISVEHMKQLDTFEGVKRRFTEKEIGSQIVIDDYAHHPKEISV
TIESARKKYPNKNVVAIFQPHTFTRTKTFLKEFADSLNEADNVYLCDIFKSAREDSGQLTINDLQKLIPNSQLLSLKETE
VLQEFKDSVLIFMGAGDIQKFQAAYEETTTKSTNFQD

Specific function: Cell wall formation

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786279, Length=464, Percent_Identity=28.2327586206897, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790680, Length=399, Percent_Identity=26.3157894736842, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURC_OCEIH (Q8EP84)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693152.1
- ProteinModelPortal:   Q8EP84
- SMR:   Q8EP84
- GeneID:   1015531
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2231
- NMPDR:   fig|221109.1.peg.2229
- HOGENOM:   HBG476594
- OMA:   YQPHRYS
- ProtClustDB:   PRK00421
- BioCyc:   OIHE221109:OB2231-MONOMER
- BRENDA:   6.3.2.8
- GO:   GO:0005737
- HAMAP:   MF_00046
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR005758
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01082

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.8

Molecular weight: Translated: 49863; Mature: 49732

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYHFIGIKGTGMSALAQILHDSGEKVQGSDFEKRFFTQEALEQKNITILPFSKENIKE
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHCCCC
DYTIIAGNAFSDDHIEIQKAKELGCKFYRYHEFLGEWLKQYTSIAVTGAHGKTSTTGLLS
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHH
HVLKSAYPISYLIGDGTGNGHVDSEYFVFEACEYRRHFLKYEPDYAIMTNIDFDHPDYFT
HHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
SLDDVVDAFQSMADRVKKCIVACGDDEQLQGIHTKVPVIYYGFNDSNDFQAQNVVETANG
HHHHHHHHHHHHHHHHHHHEEECCCCHHHCCCEEECCEEEEECCCCCCCCHHHHHHCCCC
TEFDVFVRNTYYDHFIIPTYGNHTVLNALAVIAICHYEGISVEHMKQLDTFEGVKRRFTE
CEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KEIGSQIVIDDYAHHPKEISVTIESARKKYPNKNVVAIFQPHTFTRTKTFLKEFADSLNE
HHHCCEEEEECCCCCCCEEEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
ADNVYLCDIFKSAREDSGQLTINDLQKLIPNSQLLSLKETEVLQEFKDSVLIFMGAGDIQ
CCCEEEEHHHHHCCCCCCCEEHHHHHHHCCCCCEECHHHHHHHHHHCCCEEEEEECCCHH
KFQAAYEETTTKSTNFQD
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTYHFIGIKGTGMSALAQILHDSGEKVQGSDFEKRFFTQEALEQKNITILPFSKENIKE
CEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHCCCC
DYTIIAGNAFSDDHIEIQKAKELGCKFYRYHEFLGEWLKQYTSIAVTGAHGKTSTTGLLS
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHH
HVLKSAYPISYLIGDGTGNGHVDSEYFVFEACEYRRHFLKYEPDYAIMTNIDFDHPDYFT
HHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
SLDDVVDAFQSMADRVKKCIVACGDDEQLQGIHTKVPVIYYGFNDSNDFQAQNVVETANG
HHHHHHHHHHHHHHHHHHHEEECCCCHHHCCCEEECCEEEEECCCCCCCCHHHHHHCCCC
TEFDVFVRNTYYDHFIIPTYGNHTVLNALAVIAICHYEGISVEHMKQLDTFEGVKRRFTE
CEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KEIGSQIVIDDYAHHPKEISVTIESARKKYPNKNVVAIFQPHTFTRTKTFLKEFADSLNE
HHHCCEEEEECCCCCCCEEEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
ADNVYLCDIFKSAREDSGQLTINDLQKLIPNSQLLSLKETEVLQEFKDSVLIFMGAGDIQ
CCCEEEEHHHHHCCCCCCCEEHHHHHHHCCCCCEECHHHHHHHHHHCCCEEEEEECCCHH
KFQAAYEETTTKSTNFQD
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376