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The map label for this gene is queF

Identifier: 23099665

GI number: 23099665

Start: 2245193

End: 2245690

Strand: Reverse

Name: queF

Synonym: OB2210

Alternate gene names: 23099665

Gene position: 2245690-2245193 (Counterclockwise)

Preceding gene: 23099666

Following gene: 23099664

Centisome position: 61.86

GC content: 33.13

Gene sequence:

>498_bases
ATGGTGGGAAGAGATGAAAATGAACTACAAGATGTTCAGTTGTTAGGAAGTCAAGGGACTACTTATGATTTTAATTACAC
ACCGGAAGTGTTAGAGGTTTTTGATAACAAACATGTATCACGTGATTATTTTGTTAAATTTAATTGTCCAGAATTCACTA
CGTTGTGTCCAAAGACAAATCAGCCTGATTTTGGGACTATTTATATTAGCTATATTCCAGATATTAAAATGGTCGAAAGT
AAATCATTAAAATTGTACTTGTTTAGTTTTAGGAACCATGGGGATTTTCATGAGGACTGCATTAATATTATTATGAATGA
CTTAATTGATTTAATGAATCCAAGATATATTGAGGTTCGTGGAAAATTTACTCCACGTGGCGGTATTTCCATTGATCCAT
ATTGTAATTATGGTCGACCAGGCACAAAATTTGAGCAAATGGCGGATCAACGACTTATCCAACATGATATGTATCCGGAA
AAAATAGATAATCGATAG

Upstream 100 bases:

>100_bases
AGCTTCAACCCTCTATAAAAAACTAAGGACAAACAATGATTTATTCAAGTCATGTTCCTTAGGGGACATGACTTTTTTTA
TGGAAAAGGAGGTAATAAGT

Downstream 100 bases:

>100_bases
AGGGAAAGTGTAAGATCCTCTAGCCTAATAAAGGGGAAGAGGACATTATGTATATTTATTTTAGTGCAATCTTTGTAGGA
TTGTTATTACTATCAAATAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MVGRDENELQDVQLLGSQGTTYDFNYTPEVLEVFDNKHVSRDYFVKFNCPEFTTLCPKTNQPDFGTIYISYIPDIKMVES
KSLKLYLFSFRNHGDFHEDCINIIMNDLIDLMNPRYIEVRGKFTPRGGISIDPYCNYGRPGTKFEQMADQRLIQHDMYPE
KIDNR

Sequences:

>Translated_165_residues
MVGRDENELQDVQLLGSQGTTYDFNYTPEVLEVFDNKHVSRDYFVKFNCPEFTTLCPKTNQPDFGTIYISYIPDIKMVES
KSLKLYLFSFRNHGDFHEDCINIIMNDLIDLMNPRYIEVRGKFTPRGGISIDPYCNYGRPGTKFEQMADQRLIQHDMYPE
KIDNR
>Mature_165_residues
MVGRDENELQDVQLLGSQGTTYDFNYTPEVLEVFDNKHVSRDYFVKFNCPEFTTLCPKTNQPDFGTIYISYIPDIKMVES
KSLKLYLFSFRNHGDFHEDCINIIMNDLIDLMNPRYIEVRGKFTPRGGISIDPYCNYGRPGTKFEQMADQRLIQHDMYPE
KIDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=113, Percent_Identity=32.7433628318584, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_OCEIH (Q8EPA3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693131.1
- GeneID:   1015549
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2210
- NMPDR:   fig|221109.1.peg.2208
- HOGENOM:   HBG294103
- OMA:   CINTILL
- ProtClustDB:   PRK13258
- BioCyc:   OIHE221109:OB2210-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 19387; Mature: 19387

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGRDENELQDVQLLGSQGTTYDFNYTPEVLEVFDNKHVSRDYFVKFNCPEFTTLCPKTN
CCCCCCCHHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCC
QPDFGTIYISYIPDIKMVESKSLKLYLFSFRNHGDFHEDCINIIMNDLIDLMNPRYIEVR
CCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
GKFTPRGGISIDPYCNYGRPGTKFEQMADQRLIQHDMYPEKIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MVGRDENELQDVQLLGSQGTTYDFNYTPEVLEVFDNKHVSRDYFVKFNCPEFTTLCPKTN
CCCCCCCHHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCC
QPDFGTIYISYIPDIKMVESKSLKLYLFSFRNHGDFHEDCINIIMNDLIDLMNPRYIEVR
CCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
GKFTPRGGISIDPYCNYGRPGTKFEQMADQRLIQHDMYPEKIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376